[go: up one dir, main page]

RU2018101666A - Новые ферменты и системы crispr - Google Patents

Новые ферменты и системы crispr Download PDF

Info

Publication number
RU2018101666A
RU2018101666A RU2018101666A RU2018101666A RU2018101666A RU 2018101666 A RU2018101666 A RU 2018101666A RU 2018101666 A RU2018101666 A RU 2018101666A RU 2018101666 A RU2018101666 A RU 2018101666A RU 2018101666 A RU2018101666 A RU 2018101666A
Authority
RU
Russia
Prior art keywords
cell
interest
effector protein
cpf1
plant
Prior art date
Application number
RU2018101666A
Other languages
English (en)
Other versions
RU2018101666A3 (ru
RU2737537C2 (ru
Inventor
Фэн ЧЖАН
Бернд ЦЕТЧЕ
Йонатан С. ГУТЕНБЕРГ
Омар О. АБУДАЙЕ
Йан СЛЕЙМЕЙКЕР
Original Assignee
Те Брод Инститьют Инк.
Массачусетс Инститьют Оф Текнолоджи
Президент Энд Феллоуз Оф Харвард Коллидж
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55274964&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RU2018101666(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Те Брод Инститьют Инк., Массачусетс Инститьют Оф Текнолоджи, Президент Энд Феллоуз Оф Харвард Коллидж filed Critical Те Брод Инститьют Инк.
Publication of RU2018101666A publication Critical patent/RU2018101666A/ru
Publication of RU2018101666A3 publication Critical patent/RU2018101666A3/ru
Application granted granted Critical
Publication of RU2737537C2 publication Critical patent/RU2737537C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/06—Antiglaucoma agents or miotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Ophthalmology & Optometry (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Neurology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (62)

1. Сконструированная не встречающаяся в природе система на основе коротких палиндромных повторов, регулярно расположенных группами (CRISPR)-CRISPR-ассоциированного фермента (Cas) (CRISPR-Cas), содержащая
a) одну или несколько полинуклеотидных последовательностей CRISPR-Cas V типа, предусматривающих направляющую РНК, которая содержит направляющую последовательность, связанную с последовательностью прямого повтора, где направляющая последовательность способна гибридизироваться с целевой последовательностью, или одну или несколько нуклеотидных последовательностей, кодирующих одну или несколько полинуклеотидных последовательностей CRISPR-Cas V типа, и
b) эффекторный белок Cpf1 или одну или несколько нуклеотидных последовательностей, кодирующих эффекторный белок Cpf1;
где одна или несколько направляющих последовательностей гибридизируются с указанной целевой последовательностью, причем указанная целевая последовательность находится в направлении 3' от мотива, смежного с протоспейсером (PAM), и при этом указанная направляющая РНК образует комплекс с эффекторным белком Cpf1.
2. Сконструированная не встречающаяся в природе векторная система на основе коротких палиндромных повторов, регулярно расположенных группами (CRISPR)-CRISPR-ассоциированного фермента (Cas) (CRISPR-Cas), содержащая один или несколько векторов, содержащих
a) первый регуляторный элемент, функционально связанный с одной или несколькими нуклеотидными последовательностями, кодирующими одну или несколько полинуклеотидных последовательностей CRISPR-Cas V типа, предусматривающих направляющую РНК, которая содержит направляющую последовательность, связанную с последовательностью прямого повтора, где направляющая последовательность способна гибридизироваться с целевой последовательностью,
b) второй регуляторный элемент, функционально связанный с нуклеотидной последовательностью, кодирующей эффекторный белок Cpf1;
где компоненты (a) и (b) находятся в одном и том же или в разных векторах системы,
где, будучи транскрибированными, одна или несколько направляющих последовательностей гибридизируются с указанной целевой последовательностью, причем указанная целевая последовательность находится в направлении 3' от мотива, смежного с протоспейсером (PAM), и при этом указанная направляющая РНК образует комплекс с эффекторным белком Cpf1.
3. Система по п. 1 или 2, где целевые последовательности находятся в клетке.
4. Система по п. 3, где клетка предусматривает эукариотическую клетку.
5. Система по п. 1 или 2, где, будучи транскрибированными, одна или несколько направляющих последовательностей гибридизируются с целевой последовательностью, и при этом направляющая РНК образует комплекс c эффекторным белком Cpf1, который вызывает расщепление отдаленно от целевой последовательности.
6. Система по п. 5, где указанное расщепление приводит к образованию ступенчатого двухнитевого разрыва с "липким" 5'-концом длиной 4 или 5 нуклеотидов.
7. Система по п. 1 или 2, где PAM предусматривает 5'-мотив с высоким содержанием T.
8. Система по п. 1 или 2, где эффекторный белок представляет собой эффекторный белок Cpf1, происходящий от одного из видов бактерий, приведенных на фигуре 64.
9. Система по п. 8, где эффекторный белок Cpf1 происходит от вида бактерий, выбранного из группы, состоящей из Francisella tularensis 1, Francisella tularensis subsp. novicida, Prevotella albensis, Lachnospiraceae bacterium MC2017 1, Butyrivibrio proteoclasticus, Peregrinibacteria bacterium GW2011_GWA2_33_10, Parcubacteria bacterium GW2011_GWC2_44_17, Smithella sp. SCADC, Acidaminococcus sp. BV3L6, Lachnospiraceae bacterium MA2020, Candidatus Methanoplasma termitum, Eubacterium eligens, Moraxella bovoculi 237, Leptospira inadai, Lachnospiraceae bacterium ND2006, Porphyromonas crevioricanis 3, Prevotella disiens и Porphyromonas macacae.
10. Система по п. 9, где последовательность PAM представляет собой TTN, где N представляет собой A/C/G или T, а эффекторный белок представляет собой FnCpf1, или где последовательность PAM представляет собой TTTV, где V представляет собой A/C или G, а эффекторный белок представляет собой PaCpf1p, LbCpf1 или AsCpf1.
11. Система по п. 1 или 2, где эффекторный белок Cpf1 содержит один или несколько сигналов ядерной локализации.
12. Система по п. 1 или 2, где последовательности нуклеиновой кислоты, кодирующие эффекторный белок Cpf1, являются кодон-оптимизированными для экспрессии в эукариотической клетке.
13. Система по п. 1 или 2, где компоненты (a) и (b) или нуклеотидные последовательности находятся в одном векторе.
14. Способ модифицирования представляющего интерес целевого локуса, включающий доставку системы по п. 1 или 2 в указанный локус или клетку, содержащую локус.
15. Способ модифицирования представляющего интерес целевого локуса, причем способ включает доставку в указанный локус не встречающейся в природе или сконструированной композиции, содержащей эффекторный белок Cpf1 и один или несколько компонентов на основе нуклеиновой кислоты, где эффекторный белок Cpf1 образует комплекс с одним или несколькими компонентами на основе нуклеиновой кислоты, и после связывания указанного комплекса с представляющим интерес целевым локусом, который находится в направлении 3' от мотива, смежного с протоспейсером (PAM), эффекторный белок индуцирует модификацию представляющего интерес целевого локуса.
16. Способ по п. 15, где представляющий интерес целевой локус находится в клетке.
17. Способ по п. 16, где клетка является эукариотической клеткой.
18. Способ по п. 16, где клетка является клеткой животного или человека.
19. Способ по п. 16, где клетка является растительной клеткой.
20. Способ по п. 15, где представляющий интерес целевой локус содержится в молекуле ДНК in vitro.
21. Способ по п. 15, где указанную не встречающуюся в природе или сконструированную композицию, содержащую эффекторный белок Cpf1 и один или несколько компонентов на основе нуклеиновой кислоты, доставляют в клетку в виде одной или нескольких полинуклеотидных молекул.
22. Способ по п. 15, где представляющий интерес целевой локус предусматривает ДНК.
23. Способ по п. 22, где ДНК является релаксированной или суперспирализованной.
24. Способ по п. 15, где композиция содержит один компонент на основе нуклеиновой кислоты.
25. Способ по п. 24, где один компонент на основе нуклеиновой кислоты предусматривает направляющую последовательность, связанную с последовательностью прямого повтора.
26. Способ по п. 15, где модификация представляющего интерес целевого локуса представляет собой разрыв нити.
27. Способ по п. 26, где разрыв нити предусматривает ступенчатый двухнитевой разрыв ДНК с "липким" 5'-концом длиной 4 или 5 нуклеотидов.
28. Способ по п. 26, где представляющий интерес целевой локус является модифицированным посредством интеграции ДНК-вставки в ступенчатый двухнитевой разрыв ДНК.
29. Способ по п. 15, где эффекторный белок Cpf1 содержит один или несколько сигналов ядерной локализации (NLS).
30. Способ по п. 21, где одна или несколько полинуклеотидных молекул содержатся в одном или нескольких векторах.
31. Способ по п. 21, где одна или несколько полинуклеотидных молекул содержат один или несколько регуляторных элементов, функционально сконфигурированных для обеспечения экспрессии эффекторного белка Cpf1 и/или компонента(компонентов) на основе нуклеиновой кислоты, где один или несколько регуляторных элементов необязательно предусматривают индуцируемые промоторы.
32. Способ по п. 21, где одна или несколько полинуклеотидных молекул или один или несколько векторов содержатся в системе доставки.
33. Способ по п. 21, где систему или одну или несколько полинуклеотидных молекул доставляют посредством частиц, везикул или одного или нескольких вирусных векторов.
34. Способ по п. 33, где частицы предусматривают липид, сахар, металл или белок.
35. Способ по п. 33, где везикулы предусматривают экзосомы или липосомы.
36. Способ по п. 33, где один или несколько вирусных векторов предусматривают одно или несколько из аденовируса, одного или нескольких лентивирусов или одного или нескольких аденоассоциированных вирусов.
37. Способ по п. 15, который представляет собой способ модифицирования клетки, линии клеток или организма путем манипуляции с одной или несколькими целевыми последовательностями в представляющих интерес локусах генома.
38. Клетка, полученная в результате осуществления способа по п. 37, или ее потомство, где клетка содержит модификацию, не присутствующую в клетке, в отношении которой не осуществляли способ.
39. Клетка по п. 38 или ее потомство, где клетка, в отношении которой не осуществляли способ, содержит аномалию, а клетка, полученная в результате осуществления способа, характеризуется устраненной или скорректированной аномалией.
40. Продукт клетки, полученный из клетки или ее потомства по п. 38, где продукт является модифицированным по своей природе или количеству по сравнению с продуктом клетки, полученным из клетки, в отношении которой не осуществляли способ.
41. Продукт клетки по п. 40, где клетка, в отношении которой не осуществляли способ, содержит аномалию, и при этом продукт клетки отражает аномалию, которая устраняется или корректируется с помощью способа.
42. In vitro, ex vivo или in vivo клетка-хозяин или линия клеток или их потомство, содержащие систему по п. 1 или 2.
43. Клетка-хозяин или линия клеток или их потомство по п. 42, где клетка является эукариотической клеткой.
44. Клетка-хозяин или линия клеток или их потомство по п. 43, где клетка является клеткой животного.
45. Клетка-хозяин или линия клеток или их потомство по п. 33, где клетка является клеткой человека.
46. Клетка-хозяин, линия клеток или их потомство по п. 31, предусматривающие стволовую клетку или линию стволовых клеток.
47. Клетка-хозяин или линия клеток или их потомство по п. 30, где клетка является растительной клеткой.
48. Способ получения растения с модифицированным представляющим интерес признаком, кодируемым представляющим интерес геном, причем указанный способ включает приведение растительной клетки в контакт с системой по п. 1 или п. 2 или осуществление в отношении растительной клетки способа по п. 15, за счет чего обеспечивается либо модифицирование, либо введение указанного представляющего интерес гена, и регенерацию растения из указанной растительной клетки.
49. Способ идентификации представляющего интерес признака у растения, причем указанный представляющий интерес признак кодируется представляющим интерес геном, причем указанный способ включает приведение растительной клетки в контакт с системой по п. 1 или 2 или осуществление в отношении растительной клетки способа по п. 15, за счет чего обеспечивается идентификация указанного представляющего интерес гена.
50. Способ по п. 49, дополнительно включающий введение идентифицированного представляющего интерес гена в растительную клетку, или линию растительных клеток, или растительную зародышевую плазму и получение из них растения, в результате чего растение содержит представляющий интерес ген.
51. Способ по п. 50, где у растения проявляется представляющий интерес признак.
52. Частица, содержащая систему по п. 1 или 2.
53. Частица по п. 52, где частица содержит эффекторный белок Cpf1 в комплексе с направляющей РНК.
54. Система или способ по пп. 1, 2 или 15, где комплекс, направляющая РНК или белок конъюгированы по меньшей мере с одним сахарным фрагментом, необязательно N-ацетилгалактозамином (GalNAc), в частности, с трехразветвленным GalNAc.
55. Система или способ по пп. 1, 2 или 15, где концентрация Mg2+ составляет от приблизительно 1 мМ до приблизительно 15 мМ.
RU2018101666A 2015-06-18 2016-06-17 Новые ферменты и системы crispr RU2737537C2 (ru)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201562181739P 2015-06-18 2015-06-18
US62/181,739 2015-06-18
US201562193507P 2015-07-16 2015-07-16
US62/193,507 2015-07-16
US201562201542P 2015-08-05 2015-08-05
US62/201,542 2015-08-05
US201562205733P 2015-08-16 2015-08-16
US62/205,733 2015-08-16
US201562232067P 2015-09-24 2015-09-24
US62/232,067 2015-09-24
US14/975,085 2015-12-18
US14/975,085 US9790490B2 (en) 2015-06-18 2015-12-18 CRISPR enzymes and systems
EP16150428.7A EP3009511B2 (en) 2015-06-18 2016-01-07 Novel crispr enzymes and systems
EP16150428.7 2016-01-07
PCT/US2016/038181 WO2016205711A1 (en) 2015-06-18 2016-06-17 Novel crispr enzymes and systems

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2020136363A Division RU2792654C2 (ru) 2015-06-18 2016-06-17 Новые ферменты и системы crispr

Publications (3)

Publication Number Publication Date
RU2018101666A true RU2018101666A (ru) 2019-07-19
RU2018101666A3 RU2018101666A3 (ru) 2020-04-28
RU2737537C2 RU2737537C2 (ru) 2020-12-01

Family

ID=55274964

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2018101666A RU2737537C2 (ru) 2015-06-18 2016-06-17 Новые ферменты и системы crispr

Country Status (16)

Country Link
US (2) US9790490B2 (ru)
EP (6) EP3009511B2 (ru)
JP (4) JP6898865B2 (ru)
KR (2) KR102613296B1 (ru)
CN (6) CN108513582B (ru)
AU (3) AU2016278990B2 (ru)
CA (1) CA2989834A1 (ru)
DK (5) DK3009511T3 (ru)
ES (4) ES2646140T3 (ru)
FI (2) FI3310917T4 (ru)
IL (3) IL290678B2 (ru)
MX (2) MX377162B (ru)
RU (1) RU2737537C2 (ru)
SG (1) SG10201912330QA (ru)
TW (2) TWI758251B (ru)
WO (1) WO2016205711A1 (ru)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2720769C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/CAS и препарат для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
RU2720768C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
RU2720767C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ обнаружения провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях и специфические олигонуклеотиды для использования в способе
RU2743861C1 (ru) * 2020-04-15 2021-03-01 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для выявления гена антибиотикоустойчивости blaVIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
RU2745637C1 (ru) * 2020-04-15 2021-03-29 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/Cas и препарат для выявления гена антибиотикоустойчивости bla VIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях

Families Citing this family (836)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
EP3597741A1 (en) 2012-04-27 2020-01-22 Duke University Genetic correction of mutated genes
CN113563476A (zh) 2013-03-15 2021-10-29 通用医疗公司 遗传和表观遗传调节蛋白至特定基因组基因座的rna引导的靶向
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
HRP20181648T1 (hr) 2013-04-16 2019-01-25 Regeneron Pharmaceuticals, Inc. Ciljana modifikacija genoma štakora
EP3011034B1 (en) * 2013-06-17 2019-08-07 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
DK3066201T3 (en) 2013-11-07 2018-06-06 Editas Medicine Inc CRISPR-RELATED PROCEDURES AND COMPOSITIONS WITH LEADING GRADES
MX388127B (es) 2013-12-11 2025-03-19 Regeneron Pharma Metodos y composiciones para la modificacion dirigida de un genoma.
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
EP3985114A1 (en) 2014-02-11 2022-04-20 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
JP6779866B2 (ja) 2014-06-13 2020-11-04 チルドレンズ メディカル センター コーポレイション ミトコンドリアを単離するための製品および方法
ES2694629T3 (es) 2014-06-26 2018-12-26 Regeneron Pharmaceuticals, Inc. Métodos y composiciones para modificaciones genéticas objetivo y métodos de uso
WO2021178933A2 (en) * 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Class ii, type v crispr systems
EP4079847A1 (en) 2014-07-30 2022-10-26 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
CN107002042A (zh) 2014-10-15 2017-08-01 瑞泽恩制药公司 用于产生或维持多能细胞的方法和组合物
US11680268B2 (en) 2014-11-07 2023-06-20 Editas Medicine, Inc. Methods for improving CRISPR/Cas-mediated genome-editing
RU2734770C2 (ru) 2014-11-21 2020-10-23 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленной генетической модификации с использованием парных гидовых рнк
CN107249318A (zh) 2014-12-10 2017-10-13 明尼苏达大学董事会 用于治疗疾病的遗传修饰的细胞、组织和器官
MX388784B (es) 2014-12-19 2025-03-20 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa.
WO2016130600A2 (en) 2015-02-09 2016-08-18 Duke University Compositions and methods for epigenome editing
ES3030433T3 (en) * 2015-03-31 2025-06-30 Sohm Inc Cas 9 retroviral integrase systems for targeted incorporation of a dna sequence into a genome of a cell
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
AU2016261358B2 (en) 2015-05-11 2021-09-16 Editas Medicine, Inc. Optimized CRISPR/Cas9 systems and methods for gene editing in stem cells
EP3294879A4 (en) * 2015-05-14 2019-02-20 University of Southern California OPTIMIZED GENETIZATION WITH A RECOMBINANT ENDONUCLEASE SYSTEM
EP3307887A1 (en) 2015-06-09 2018-04-18 Editas Medicine, Inc. Crispr/cas-related methods and compositions for improving transplantation
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
EP3666895A1 (en) 2015-06-18 2020-06-17 The Broad Institute, Inc. Novel crispr enzymes and systems
CA3012607A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzymes and systems
AU2016285724A1 (en) 2015-06-29 2017-11-16 Ionis Pharmaceuticals, Inc. Modified CRISPR RNA and modified single CRISPR RNA and uses thereof
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
EP3328399B1 (en) 2015-07-31 2023-12-27 Regents of the University of Minnesota Modified cells and methods of therapy
EP3337908A4 (en) 2015-08-18 2019-01-23 The Broad Institute, Inc. METHOD AND COMPOSITIONS FOR CHANGING THE FUNCTION AND STRUCTURE OF CHROMATIN GRINDING AND / OR DOMAINS
US11530424B1 (en) * 2015-09-18 2022-12-20 University Of South Florida Efficient CRISPR/HDR-mediated knock-in system and method of use
US20190048340A1 (en) * 2015-09-24 2019-02-14 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
CA2999500A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
AU2016333886B2 (en) 2015-10-05 2020-10-08 Precision Biosciences, Inc. Engineered meganucleases with recognition sequences found in the human T cell receptor alpha constant region gene
KR20250013291A (ko) 2015-10-05 2025-01-31 프리시젼 바이오사이언시스 인코포레이티드 변형된 인간 t 세포 수용체 알파 불변 영역 유전자를 포함하는 유전자 변형된 세포
AU2016335698B2 (en) 2015-10-08 2022-12-01 President And Fellows Of Harvard College Multiplexed genome editing
AU2016334225B2 (en) * 2015-10-09 2022-11-10 Monsanto Technology Llc Novel RNA-guided nucleases and uses thereof
EP4089175A1 (en) 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells
CA3001130A1 (en) * 2015-10-16 2017-04-20 Temple University - Of The Commonwealth System Of Higher Education Methods and compositions utilizing cpf1 for rna-guided gene editing
JP7067793B2 (ja) 2015-10-23 2022-05-16 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基編集因子およびその使用
DK3350327T3 (en) 2015-10-23 2019-01-21 Caribou Biosciences Inc CONSTRUCTED CRISPR CLASS-2-NUCLEIC ACID TARGETING-NUCLEIC ACID
US20180230489A1 (en) 2015-10-28 2018-08-16 Voyager Therapeutics, Inc. Regulatable expression using adeno-associated virus (aav)
CA3004497A1 (en) * 2015-11-06 2017-05-11 The Jackson Laboratory Large genomic dna knock-in and uses thereof
US11905521B2 (en) * 2015-11-17 2024-02-20 The Chinese University Of Hong Kong Methods and systems for targeted gene manipulation
WO2017095940A1 (en) 2015-11-30 2017-06-08 Flagship Pioneering, Inc. Methods and compositions relating to chondrisomes from cultured cells
EP4644567A2 (en) 2015-11-30 2025-11-05 Duke University Therapeutic targets for the correction of the human dystrophin gene by gene editing and methods of use
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
BR112018011503A2 (pt) 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
WO2017099494A1 (ko) * 2015-12-08 2017-06-15 기초과학연구원 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
CA3009190A1 (en) 2015-12-29 2017-07-06 Monsanto Technology Llc Novel crispr-associated transposases and uses thereof
US11427837B2 (en) * 2016-01-12 2022-08-30 The Regents Of The University Of California Compositions and methods for enhanced genome editing
CN109152795A (zh) 2016-01-15 2019-01-04 儿童医学中心公司 线粒体和组合的线粒体试剂的治疗用途
WO2017127807A1 (en) * 2016-01-22 2017-07-27 The Broad Institute Inc. Crystal structure of crispr cpf1
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
WO2017151719A1 (en) * 2016-03-01 2017-09-08 University Of Florida Research Foundation, Incorporated Molecular cell diary system
WO2017152015A1 (en) * 2016-03-04 2017-09-08 Editas Medicine, Inc. Crispr-cpf1-related methods, compositions and components for cancer immunotherapy
EP3433363A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
WO2017180915A2 (en) 2016-04-13 2017-10-19 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
US11286478B2 (en) 2016-04-19 2022-03-29 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
KR20260004568A (ko) 2016-04-19 2026-01-08 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
CA3026110A1 (en) 2016-04-19 2017-11-02 The Broad Institute, Inc. Novel crispr enzymes and systems
EP3450570B1 (en) * 2016-04-28 2021-03-10 Industry-Academic Cooperation Foundation, Yonsei University Method for evaluating, in vivo, activity of rna-guided nuclease in high-throughput manner
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
EP3456825A4 (en) * 2016-05-13 2020-03-18 Kaneka Corporation Plant genome editing method
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
JP6967217B2 (ja) 2016-05-13 2021-11-17 株式会社カネカ 形質転換植物の作製方法
RU2745563C2 (ru) 2016-05-20 2021-03-29 Регенерон Фармасьютикалс, Инк. Способы преодоления иммунологической толерантности с использованием множества направляющих рнк
EA201892810A1 (ru) 2016-06-01 2019-06-28 Квс Заат Се Гибридные последовательности нуклеиновых кислот для геномной инженерии
WO2017210380A1 (en) * 2016-06-01 2017-12-07 Excision Biotherapeutics, Inc. Compositions and methods of treatment for lytic and lysogenic viruses
PL3604527T3 (pl) * 2016-06-02 2021-09-06 Sigma-Aldrich Co., Llc Zastosowanie programowalnych białek wiążących dna do wzmocnienia celowanej modyfikacji genomu
BR112018076027A2 (pt) 2016-06-14 2019-03-26 Pioneer Hi-Bred International, Inc. método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
CN109642231A (zh) * 2016-06-17 2019-04-16 博德研究所 Vi型crispr直向同源物和系统
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
EP3474669B1 (en) * 2016-06-24 2022-04-06 The Regents of The University of Colorado, A Body Corporate Methods for generating barcoded combinatorial libraries
KR102345899B1 (ko) 2016-06-30 2021-12-31 지머젠 인코포레이티드 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도
WO2018005793A1 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
CN106191057B (zh) * 2016-07-06 2018-12-25 中山大学 一种用于敲除人CYP2E1基因的sgRNA序列、CYP2E1基因缺失细胞株的构建方法及其应用
WO2018013720A1 (en) * 2016-07-12 2018-01-18 Washington University Incorporation of internal polya-encoded poly-lysine sequence tags and their variations for the tunable control of protein synthesis in bacterial and eukaryotic cells
EP4321623A3 (en) * 2016-07-15 2024-05-15 Salk Institute for Biological Studies Methods and compositions for genome editing in non-dividing cells
WO2018017754A1 (en) * 2016-07-19 2018-01-25 Duke University Therapeutic applications of cpf1-based genome editing
KR20230088522A (ko) 2016-07-21 2023-06-19 맥스시티 인코포레이티드 게놈 dna를 변경하기 위한 방법 및 조성물
EP3491133A4 (en) 2016-07-26 2020-05-06 The General Hospital Corporation VARIANTS OF CRISPR OF PREVOTELLA AND FRANCISELLA 1 (CPF1)
IL264565B2 (en) 2016-08-03 2024-07-01 Harvard College Adenosine nucleobase editors and uses thereof
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
EP3497214B1 (en) 2016-08-09 2023-06-28 President and Fellows of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
TWI874884B (zh) * 2016-08-10 2025-03-01 日商領先基因生技股份有限公司 檢測標的部位中的檢測對象核酸序列之存在或不存在之方法
US11352647B2 (en) 2016-08-17 2022-06-07 The Broad Institute, Inc. Crispr enzymes and systems
CN110312799A (zh) * 2016-08-17 2019-10-08 博德研究所 新型crispr酶和系统
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
IL247752A0 (en) * 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
US10650621B1 (en) 2016-09-13 2020-05-12 Iocurrents, Inc. Interfacing with a vehicular controller area network
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
KR20190071725A (ko) 2016-09-30 2019-06-24 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Rna-가이드된 핵산 변형 효소 및 이의 사용 방법
MX2019003678A (es) * 2016-09-30 2020-08-13 Univ California Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas.
AU2017338827B2 (en) 2016-10-03 2023-08-31 Juno Therapeutics, Inc. HPV-specific binding molecules
CA3039014A1 (en) 2016-10-04 2018-04-12 Precision Biosciences, Inc. Co-stimulatory domains for use in genetically-modified cells
US11242542B2 (en) 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same
US10717978B2 (en) 2016-10-07 2020-07-21 Integrated Dna Technologies, Inc. S. pyogenes CAS9 mutant genes and polypeptides encoded by same
WO2018071672A1 (en) * 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases
EP3525832A4 (en) * 2016-10-14 2020-04-29 The General Hospital Corp. Epigenetically regulated site-specific nucleases
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
AU2017346885B2 (en) 2016-10-18 2021-10-21 Intima Bioscience, Inc. Tumor infiltrating lymphocytes and methods of therapy
US20190264218A1 (en) 2016-11-04 2019-08-29 Flagship Pioneering Innovations V, Inc. Novel Plant Cells, Plants, and Seeds
CN106591335A (zh) * 2016-11-08 2017-04-26 安徽省农业科学院水稻研究所 一种密码子植物化改造的LbCpf1基因及其应用
EP3545085A4 (en) * 2016-11-22 2020-10-28 Integrated Dna Technologies, Inc. CRISPR / CPF1 SYSTEMS AND METHODS
CA3044531A1 (en) * 2016-11-28 2018-05-31 The Board Of Regents Of The University Of Texas System Prevention of muscular dystrophy by crispr/cpf1-mediated gene editing
CN108130342B (zh) * 2016-12-01 2022-01-11 中国科学院分子植物科学卓越创新中心 基于Cpf1的植物基因组定点编辑方法
US12404514B2 (en) * 2016-12-09 2025-09-02 The Broad Institute, Inc. CRISPR-systems for modifying a trait of interest in a plant
US11293022B2 (en) 2016-12-12 2022-04-05 Integrated Dna Technologies, Inc. Genome editing enhancement
IL267034B2 (en) 2016-12-14 2025-03-01 Ligandal Inc Methods and compositions for nucleic acid and protein payload delivery
US20190346460A1 (en) * 2016-12-19 2019-11-14 The Uab Research Foundation Mass spectrometric standards for hemoglobin beta and hemoglobin beta sickle and uses thereof
US12286727B2 (en) 2016-12-19 2025-04-29 Editas Medicine, Inc. Assessing nuclease cleavage
KR102677877B1 (ko) * 2016-12-22 2024-06-25 키진 엔.브이. 이중나선 dna의 표적화된 변형 방법
GB2572918B (en) * 2016-12-23 2023-02-15 Harvard College Gene editing of PCSK9
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
US20200385753A1 (en) * 2016-12-23 2020-12-10 Institute For Basic Science Composition for base editing for animal embryo and base editing method
CA3047330A1 (en) * 2016-12-28 2018-07-05 Ionis Pharmaceuticals, Inc. Modified crispr rna and uses thereof
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
EP4095263A1 (en) 2017-01-06 2022-11-30 Editas Medicine, Inc. Methods of assessing nuclease cleavage
ES2949801T3 (es) 2017-01-09 2023-10-03 Whitehead Inst Biomedical Res Métodos para alterar la expresión génica mediante la perturbación de multímeros de factores de transcripción que estructuran bucles reguladores
GB201700380D0 (en) * 2017-01-10 2017-02-22 Plant Bioscience Ltd Methods of increasing seed yield
JP2020513783A (ja) * 2017-01-18 2020-05-21 エクシジョン バイオセラピューティクス インコーポレイテッド Ｃｒｉｓｐｒ
EP3573448A4 (en) 2017-01-28 2020-09-02 Inari Agriculture, Inc. NEW PLANT CELLS, PLANTS AND SEEDS
AU2018212624A1 (en) 2017-01-30 2019-08-22 KWS SAAT SE & Co. KGaA Repair template linkage to endonucleases for genome engineering
JP2020507312A (ja) * 2017-02-10 2020-03-12 ザイマージェン インコーポレイテッド 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略
CA3053092A1 (en) * 2017-02-10 2018-08-16 National Agriculture And Food Research Organization Method for producing hsl protein having improved catalytic activity for 2-oxoglutaric acid-dependently oxidizing 4-hppd inhibitor
WO2018149915A1 (en) * 2017-02-15 2018-08-23 Keygene N.V. Methods of targeted genetic alteration in plant cells
CN106868031A (zh) * 2017-02-24 2017-06-20 北京大学 一种基于分级组装的多个sgRNA串联并行表达的克隆方法及应用
CN106947779B (zh) * 2017-02-28 2021-02-26 中国农业大学 利用乳腺生物反应器提高乳汁中褪黑素含量的方法
US10687520B2 (en) 2017-03-07 2020-06-23 The Board Of Regents Of The University Of Texas System Generation and correction of a humanized mouse model with a deletion of dystrophin exon 44
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
EP3592777A1 (en) 2017-03-10 2020-01-15 President and Fellows of Harvard College Cytosine to guanine base editor
CN110959039B (zh) * 2017-03-15 2025-03-14 博德研究所 新型cas13b直向同源物crispr酶和系统
CN106978428A (zh) * 2017-03-15 2017-07-25 上海吐露港生物科技有限公司 一种Cas蛋白特异结合靶标DNA、调控靶标基因转录的方法及试剂盒
SG11201908658TA (en) 2017-03-23 2019-10-30 Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018175712A1 (en) * 2017-03-23 2018-09-27 The Government Of The United States Of America As Represented By The Secretary Of The Army Recombinant adeno-associated virus-paraoxonase 1-ifii particles and the methods of making and using thereof
US12161694B2 (en) 2017-03-24 2024-12-10 The Broad Institute, Inc. Methods and compositions for regulating innate lymphoid cell inflammatory responses
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
CN108690845B (zh) 2017-04-10 2021-04-27 中国科学院动物研究所 基因组编辑系统和方法
EP3609314A4 (en) 2017-04-14 2021-04-14 Monsanto Technology LLC PROCEDURES AND COMPOSITIONS FOR HERBICIDE TOLERANCE IN PLANTS
WO2018191715A2 (en) * 2017-04-14 2018-10-18 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
CN116555353A (zh) 2017-04-20 2023-08-08 E开创生物技术股份有限公司 产生基因修改的动物的方法
EP3612204A4 (en) * 2017-04-21 2021-01-27 The General Hospital Corporation INDUCTIBLE, TUNING AND MULTIPLEX HUMAN GENE REGULATION USING CRISPR-CPF1
BR112019021719A2 (pt) * 2017-04-21 2020-06-16 The General Hospital Corporation Variantes de cpf1 (cas12a) com especificidade para pam alterada
US11499151B2 (en) 2017-04-28 2022-11-15 Editas Medicine, Inc. Methods and systems for analyzing guide RNA molecules
US12029760B2 (en) * 2017-05-04 2024-07-09 The Trustees Of The University Of Pennsylvania Compositions and methods for gene editing in T cells using CRISPR/Cpf1
AU2018266698A1 (en) 2017-05-08 2019-11-28 Precision Biosciences, Inc. Nucleic acid molecules encoding an engineered antigen receptor and an inhibitory nucleic acid molecule and methods of use thereof
EP3622092A4 (en) 2017-05-11 2021-06-23 The Broad Institute, Inc. METHODS AND COMPOSITIONS OF USE OF CD8 + TUMOR-INFILTRATING LYMPHOCYTE SUBTYPES AND GENE SIGNATURES THEREOF
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
KR102678809B1 (ko) * 2017-05-18 2024-06-26 더 브로드 인스티튜트, 인코퍼레이티드 표적화된 핵산 편집을 위한 시스템, 방법 및 조성물
WO2018213726A1 (en) 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
US11046969B2 (en) 2017-05-24 2021-06-29 Epiplanta Biotech Ltd. Transgenic plant and the method for producing the same
JP7191042B2 (ja) * 2017-05-25 2022-12-16 ２セブンティ バイオ インコーポレイテッド Ｃｂｌｂエンドヌクレアーゼバリアント、組成物、および使用方法
WO2018226762A1 (en) * 2017-06-05 2018-12-13 Fred Hutchinson Cancer Research Center Genomic safe harbors for genetic therapies in human stem cells and engineered nanoparticles to provide targeted genetic therapies
CA3064612A1 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving escherichia coli
CN110719956A (zh) 2017-06-06 2020-01-21 齐默尔根公司 用于改良真菌菌株的高通量基因组工程改造平台
JP7518620B2 (ja) 2017-06-09 2024-07-18 エディタス・メディシン，インコーポレイテッド 操作されたｃａｓ９ヌクレアーゼ
CN111108208A (zh) 2017-06-13 2020-05-05 旗舰先锋创新V股份有限公司 包含愈合子的组合物及其用途
US11897953B2 (en) 2017-06-14 2024-02-13 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
CN111511906A (zh) * 2017-06-23 2020-08-07 因思科瑞普特公司 核酸引导性核酸酶
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
CN111328290A (zh) 2017-06-26 2020-06-23 博德研究所 用于靶向核酸编辑的基于crispr/cas-腺嘌呤脱氨酶的组合物、系统和方法
CN107312790A (zh) * 2017-06-26 2017-11-03 中国科学技术大学 一种可编程的多位点特异性的转录抑制系统及其应用
JP2020529834A (ja) * 2017-06-30 2020-10-15 プレシジョン バイオサイエンシズ，インク． Ｔ細胞受容体アルファ遺伝子の改変されたイントロンを含む遺伝子改変ｔ細胞
KR20220104847A (ko) 2017-06-30 2022-07-26 인스크립타 인코포레이티드 자동 세포 처리 방법, 모듈, 기기 및 시스템
US11168322B2 (en) 2017-06-30 2021-11-09 Arbor Biotechnologies, Inc. CRISPR RNA targeting enzymes and systems and uses thereof
JP2020530307A (ja) 2017-06-30 2020-10-22 インティマ・バイオサイエンス，インコーポレーテッド 遺伝子治療のためのアデノ随伴ウイルスベクター
KR102007457B1 (ko) * 2017-06-30 2019-08-05 동국대학교 산학협력단 Cas9 단백질, 가이드 RNA 및 양친매성 펩타이드를 포함하는 나노복합체 및 이의 용도
US12415000B2 (en) 2017-07-07 2025-09-16 The Broad Institute, Inc. CRISPR system based antiviral therapy
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
US12049643B2 (en) 2017-07-14 2024-07-30 The Broad Institute, Inc. Methods and compositions for modulating cytotoxic lymphocyte activity
CN107488710B (zh) * 2017-07-14 2020-09-22 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
US20200231975A1 (en) * 2017-07-17 2020-07-23 The Broad Institute, Inc. Novel type vi crispr orthologs and systems
WO2019018440A1 (en) 2017-07-17 2019-01-24 The Broad Institute, Inc. HUMAN COLON CELL ATLAS IN GOOD HEALTH AND WITH HEMORRHAGIC RECTO-COLITIS
WO2019017988A1 (en) * 2017-07-21 2019-01-24 Arizona Board Of Regents On Behalf Of Arizona State University CRISPR FLUORESCENT GUIDED RNA (ARNFG) FOR UNDERSTANDING RNAs EXPRESSED FROM POL II PROMOTERS
EP3657980A4 (en) 2017-07-28 2021-04-28 Locus IP Company, LLC YEAST MASKS TO IMPROVE SKIN, HAIR AND SCALP HEALTH
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US20190032156A1 (en) 2017-07-31 2019-01-31 Regeneron Pharmaceuticals, Inc. Methods and compositions for assessing crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo
BR112020001364A2 (pt) 2017-07-31 2020-08-11 Regeneron Pharmaceuticals, Inc. métodos para testar e modificar a capacidade de uma crispr/cas nuclease.
CA3068072A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Methods and compositions for assessing crispr/cas-mediated disruption or excision and crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo
US12215319B2 (en) 2017-08-04 2025-02-04 Syngenta Crop Protection Ag Methods and compositions for targeted genomic insertion
EP3665279B1 (en) 2017-08-09 2023-07-19 Benson Hill, Inc. Compositions and methods for modifying genomes
US10476825B2 (en) 2017-08-22 2019-11-12 Salk Institue for Biological Studies RNA targeting methods and compositions
US11970720B2 (en) 2017-08-22 2024-04-30 Salk Institute For Biological Studies RNA targeting methods and compositions
CN118530967A (zh) 2017-08-23 2024-08-23 通用医疗公司 具有改变的PAM特异性的工程化的CRISPR-Cas9核酸酶
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019046540A1 (en) * 2017-08-31 2019-03-07 New York Genome Center, Inc. METHODS AND COMPOSITIONS COMPRISING CRISPR-CPF1 AND APPROVED GUIDING CRISPR RNAES FOR PROGRAMMABLE GENOMIC DELETIONS
CN111511388A (zh) * 2017-09-21 2020-08-07 博德研究所 用于靶向核酸编辑的系统、方法和组合物
US20190098879A1 (en) 2017-09-29 2019-04-04 Regeneron Pharmaceuticals, Inc. Non-Human Animals Comprising A Humanized TTR Locus And Methods Of Use
KR102662879B1 (ko) * 2017-09-29 2024-05-02 주식회사 툴젠 망막 기능장애 질환 치료를 위한 유전자 조작
CA3074927A1 (en) 2017-09-30 2019-04-04 Inscripta, Inc. Flow through electroporation instrumentation
CA3080546A1 (en) 2017-10-03 2019-04-11 Juno Therapeutics, Inc. Hpv-specific binding molecules
EP4269560A3 (en) 2017-10-03 2024-01-17 Precision Biosciences, Inc. Modified epidermal growth factor receptor peptides for use in genetically-modified cells
BR112020006757A2 (pt) * 2017-10-04 2020-10-06 The Broad Institute Inc. diagnóstico baseado no sistema de efetor crispr
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
CN111479916B (zh) 2017-10-20 2024-10-22 弗莱德哈钦森癌症中心 用以产生被遗传修饰来表达所选抗体的b细胞的系统和方法
WO2019089808A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Class 2 crispr/cas compositions and methods of use
EP3704239A4 (en) 2017-11-01 2021-08-18 The Regents of The University of California CASZ COMPOSITIONS AND METHODS OF USE
WO2019089913A1 (en) 2017-11-01 2019-05-09 Precision Biosciences, Inc. Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
WO2019090174A1 (en) 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
GB2568255A (en) * 2017-11-08 2019-05-15 Evox Therapeutics Ltd Exosomes comprising RNA therapeutics
WO2019094955A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas
US12221720B2 (en) 2017-11-13 2025-02-11 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
EP3710583A1 (en) * 2017-11-16 2020-09-23 Astrazeneca AB Compositions and methods for improving the efficacy of cas9-based knock-in strategies
SG11202004457XA (en) 2017-11-17 2020-06-29 Iovance Biotherapeutics Inc Til expansion from fine needle aspirates and small biopsies
CA3083118A1 (en) 2017-11-22 2019-05-31 Iovance Biotherapeutics, Inc. Expansion of peripheral blood lymphocytes (pbls) from peripheral blood
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
US12359196B2 (en) * 2017-11-28 2025-07-15 Emendobio Inc. Differential knockout of an allele of a heterozygous fibrinogen alpha chain (FGA) gene
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
MX2020005939A (es) 2017-12-08 2020-08-24 Synthetic Genomics Inc Mejoramiento de la productividad de lipidos de algas a traves de la modificacion genetica de una proteina que contiene un dominio tpr.
US11203752B2 (en) * 2017-12-11 2021-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods of modifying a plant genome to produce a MS9, MS22, MS26, or MS45 male-sterile plant
SG11202003907WA (en) 2017-12-14 2020-05-28 Flodesign Sonics Inc Acoustic transducer drive and controller
CN120118207A (zh) 2017-12-15 2025-06-10 丹娜-法伯癌症研究院有限公司 稳定肽-介导的靶向蛋白降解
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
JP7467339B2 (ja) * 2017-12-22 2024-04-15 フェイト セラピューティクス，インコーポレイテッド 強化された免疫エフェクター細胞およびその使用
EP3728575A4 (en) 2017-12-22 2021-11-24 The Broad Institute, Inc. CAS12B SYSTEMS, METHODS AND COMPOSITIONS FOR SPECIFIC EDITING OF DNA BASES
KR102098915B1 (ko) * 2017-12-22 2020-04-09 (주)지플러스 생명과학 키메라 게놈 조작 분자 및 방법
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
CA3086620A1 (en) 2018-01-12 2019-07-18 Basf Se Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a
EP3740580A4 (en) 2018-01-19 2021-10-20 Duke University GENOME ENGINEERING WITH CRISPR-CAS SYSTEMS IN EUKARYONTS
WO2019147014A1 (ko) * 2018-01-23 2019-08-01 기초과학연구원 연장된 단일 가이드 rna 및 그 용도
US11220694B1 (en) * 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US20210047653A1 (en) * 2018-01-30 2021-02-18 The University Of Memphis Research Foundation Compositions and methods for regulating a biological process
US12275951B2 (en) * 2018-02-06 2025-04-15 Ohio State Innovation Foundation Engineered guide RNA and uses thereof
WO2019173942A1 (en) * 2018-03-12 2019-09-19 Nanjing Bioheng Biotech Co., Ltd Engineered chimeric guide rna and uses thereof
JP7457653B2 (ja) 2018-03-14 2024-03-28 アーバー バイオテクノロジーズ， インコーポレイテッド 新規ｃｒｉｓｐｒ ｄｎａターゲティング酵素及びシステム
DK3765616T3 (da) 2018-03-14 2023-08-21 Arbor Biotechnologies Inc Nye enzymer og systemer målrettet crispr dna og rna
CA3093968A1 (en) * 2018-03-15 2019-09-19 KSQ Therapeutics, Inc. Gene-regulating compositions and methods for improved immunotherapy
CN108486146B (zh) * 2018-03-16 2021-02-19 中国农业科学院作物科学研究所 LbCpf1-RR突变体用于CRISPR/Cpf1系统在植物基因编辑中的应用
EP3592140A1 (en) * 2018-03-19 2020-01-15 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems
EP3546582A1 (en) 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
CN111954715A (zh) * 2018-03-29 2020-11-17 菲特治疗公司 工程改造的免疫效应细胞和其用途
AU2019241967A1 (en) 2018-03-29 2020-11-19 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
EP3775238A1 (en) 2018-04-05 2021-02-17 Juno Therapeutics, Inc. Methods of producing cells expressing a recombinant receptor and related compositions
KR20210020873A (ko) 2018-04-05 2021-02-24 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 τ 세포, 관련 폴리뉴클레오티드 및 방법
IL304025A (en) 2018-04-12 2023-08-01 Prec Biosciences Inc Optimized engineered nucleases with specificity for the human T cell receptor alpha constant region gene
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
AU2019261361A1 (en) * 2018-04-23 2020-11-19 Duke University Downregulation of SNCA expression by targeted editing of DNA-methylation
PL3560330T3 (pl) 2018-04-24 2022-09-19 KWS SAAT SE & Co. KGaA Rośliny o ulepszonej strawności i haplotypach markerowych
US10501738B2 (en) 2018-04-24 2019-12-10 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
CN109593763B (zh) * 2018-04-27 2021-10-29 四川大学华西医院 一种FnCpf1介导的体外DNA编辑试剂盒
TWI831776B (zh) 2018-04-27 2024-02-11 美商艾歐凡斯生物治療公司 腫瘤浸潤性淋巴細胞之基因編輯和彼於免疫治療之用途
AU2019265019B2 (en) 2018-05-11 2025-11-06 Beam Therapeutics Inc. Methods of substituting pathogenic amino acids using programmable base editor systems
EP3790964A4 (en) * 2018-05-11 2022-06-08 Beam Therapeutics, Inc. METHODS FOR REMOVAL OF PATHOGENIC MUTATIONS USING PROGRAMMABLE BASE EDITOR SYSTEMS
US12522812B2 (en) 2018-05-16 2026-01-13 Arbor Biotechnologies, Inc. CRISPR-associated systems and components
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3802826A1 (en) * 2018-05-24 2021-04-14 CRISPR Therapeutics AG Methods and compositions for efficient gene deletion
WO2019232251A1 (en) * 2018-05-30 2019-12-05 The Regents Of The University Of California Gene editing of monogenic disorders in human hematopoietic stem cells -- correction of x-linked hyper-igm syndrome (xhim)
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
AU2019282094A1 (en) * 2018-06-04 2021-01-07 University Of Copenhagen Mutant Cpf1 endonucleases
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
EP3802807B1 (en) 2018-06-05 2024-11-20 LifeEDIT Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
AU2019283621B2 (en) * 2018-06-07 2023-03-30 Korea Research Institute Of Bioscience And Biotechnology Transgenic mouse for aglycosylated antibody production and use of aglycosylated antibody produced therefrom
US20210123075A1 (en) 2018-06-08 2021-04-29 Novartis Ag Compositions and methods for immunooncology
US10227576B1 (en) 2018-06-13 2019-03-12 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
US12227776B2 (en) 2018-06-13 2025-02-18 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
WO2019238911A1 (en) 2018-06-15 2019-12-19 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant ii
US12043837B2 (en) 2018-06-15 2024-07-23 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant
WO2019237395A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种应用CRISPR/Cas9系统靶向敲除人CD357基因的方法
WO2019237380A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种基于慢病毒的 CRISPR/Cpf1 基因编辑载体及其应用
EP3814520A1 (en) 2018-06-26 2021-05-05 The Broad Institute, Inc. Crispr double nickase based amplification compositions, systems, and methods
KR20210040943A (ko) 2018-06-26 2021-04-14 매사추세츠 인스티튜트 오브 테크놀로지 Crispr 이펙터 시스템 기반 증폭 방법, 시스템, 및 진단
EP3521436A1 (en) 2018-06-27 2019-08-07 VIB vzw Complex breeding in plants
CA3104856A1 (en) 2018-06-29 2020-01-02 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
US20210301288A1 (en) * 2018-07-16 2021-09-30 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
US12018297B2 (en) 2018-07-16 2024-06-25 The Regents Of The University Of Michigan Nuclease-mediated nucleic acid modification
EP4442836A3 (en) 2018-08-01 2024-12-18 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
CN113227367B (zh) * 2018-08-09 2023-05-12 G+Flas生命科学公司 用cas12a蛋白进行基因组工程的组合物和方法
AU2019319377A1 (en) * 2018-08-09 2021-03-11 G+Flas Life Sciences Novel CRISPR-associated protein and use thereof
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
WO2020037142A1 (en) 2018-08-17 2020-02-20 Yale University Compositions and methods for high-throughput activation screening to boost t cell effector function
WO2020041380A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
AR116016A1 (es) 2018-08-24 2021-03-25 Flagship Pioneering Innovations Vi Llc Métodos para fabricar paquetes mensajeros vegetales
MA53445A (fr) * 2018-08-24 2021-12-01 Flagship Pioneering Innovations Vi Llc Procédés et compositions pour la modification de plantes
US11965154B2 (en) 2018-08-30 2024-04-23 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US12534743B2 (en) 2018-08-31 2026-01-27 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US11479762B1 (en) 2018-08-31 2022-10-25 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2020051507A1 (en) 2018-09-06 2020-03-12 The Broad Institute, Inc. Nucleic acid assemblies for use in targeted delivery
CN109402115B (zh) * 2018-09-06 2024-02-02 广州普世利华科技有限公司 靶向Rett突变基因RNA的gRNA及Rett突变基因的检测方法、检测试剂盒
US12454694B2 (en) 2018-09-07 2025-10-28 Beam Therapeutics Inc. Compositions and methods for improving base editing
US12516304B2 (en) 2018-09-07 2026-01-06 Astrazeneca Ab Compositions and methods for improved nucleases
AU2019336245A1 (en) 2018-09-07 2021-04-01 Beam Therapeutics Inc. Compositions and methods for delivering a nucleobase editing system
EP3849565A4 (en) 2018-09-12 2022-12-28 Fred Hutchinson Cancer Research Center REDUCTION OF CD33 EXPRESSION TO SELECTIVELY PROTECT THERAPEUTIC CELLS
US11459552B2 (en) 2018-09-13 2022-10-04 The Board Of Regents Of The University Of Oklahoma Variant CAS12 proteins with improved dna cleavage selectivity and methods of use
WO2020055941A1 (en) * 2018-09-13 2020-03-19 Excision Biotherapeutics, Inc. Compositions and methods for excision with single grna
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
MX2021004277A (es) 2018-10-18 2021-09-08 Intellia Therapeutics Inc Composiciones y metodos para expresar el factor ix.
US10604746B1 (en) 2018-10-22 2020-03-31 Inscripta, Inc. Engineered enzymes
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
CN111100876A (zh) * 2018-10-25 2020-05-05 立沃生物科技（深圳）有限公司 CRISPR-Cas9特异性敲除FAH基因的方法及特异性sgRNA
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US20210388389A1 (en) 2018-10-30 2021-12-16 Yale University Compositions and methods for rapid and modular generation of chimeric antigen receptor t cells
SG11202104409YA (en) 2018-10-31 2021-05-28 Zymergen Inc Multiplexed deterministic assembly of dna libraries
JP7487939B2 (ja) * 2018-11-01 2024-05-21 国立大学法人 東京大学 ２分割されたＣｐｆ１タンパク質
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
CN112969469A (zh) 2018-11-05 2021-06-15 艾欧凡斯生物治疗公司 抗pd-1抗体难治性nsclc患者的治疗
CA3118493A1 (en) 2018-11-05 2020-05-14 Iovance Biotherapeutics, Inc. Expansion of tils utilizing akt pathway inhibitors
US20230039976A1 (en) 2018-11-05 2023-02-09 Iovance Biotherapeutics, Inc. Selection of improved tumor reactive t-cells
AU2019374761A1 (en) 2018-11-05 2021-06-10 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
CN109385451B (zh) * 2018-11-07 2020-07-07 中国海洋大学 一种牡蛎CRISPR/Cas9基因编辑方法
CN118497276A (zh) * 2018-11-07 2024-08-16 阿库斯股份有限公司 腺相关病毒载体用于在内耳中的毛细胞和支持细胞中校正基因缺陷/表达蛋白质的用途
AU2019375914A1 (en) * 2018-11-08 2021-06-03 Arizona Board of Regents on Behalf Arizona State University Synthetic immunomodulation with a CRISPR super-repressor in vivo
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
US12165743B2 (en) 2018-11-09 2024-12-10 The Broad Institute, Inc. Compressed sensing for screening and tissue imaging
AU2019374883A1 (en) 2018-11-09 2021-05-20 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
EP3880817A1 (en) * 2018-11-14 2021-09-22 The Broad Institute, Inc. Crispr system based droplet diagnostic systems and methods
IL283169B2 (en) * 2018-11-15 2025-10-01 Univ China Agricultural Enzyme and crispr-cas12j system
WO2020098793A1 (zh) * 2018-11-15 2020-05-22 中国农业大学 CRISPR-Cas12a酶和系统
CN109536506B (zh) * 2018-12-10 2022-03-22 潍坊科技学院 一种调控日本沼虾生长发育的类胰岛素受体基因及其应用
CN109666662A (zh) * 2018-12-12 2019-04-23 广州普世利华科技有限公司 新型ScCas12a在核酸检测方面的应用
CN109593743A (zh) * 2018-12-12 2019-04-09 广州普世利华科技有限公司 新型CRISPR/ScCas12a蛋白及其制备方法
CN109680053A (zh) * 2018-12-12 2019-04-26 广州普世利华科技有限公司 新型SsCas12a蛋白在核酸检测方面的应用
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use
US20250043366A1 (en) 2018-12-13 2025-02-06 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
US11384344B2 (en) 2018-12-17 2022-07-12 The Broad Institute, Inc. CRISPR-associated transposase systems and methods of use thereof
US12264323B2 (en) 2018-12-17 2025-04-01 The Broad Institute, Inc. CRISPR CPF1 direct repeat variants
WO2020131547A1 (en) 2018-12-19 2020-06-25 Iovance Biotherapeutics, Inc. Methods of expanding tumor infiltrating lymphocytes using engineered cytokine receptor pairs and uses thereof
AU2019403015B2 (en) 2018-12-20 2024-01-18 Regeneron Pharmaceuticals, Inc. Nuclease-mediated repeat expansion
EP3898661A1 (en) 2018-12-21 2021-10-27 Precision BioSciences, Inc. Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria
CN113631704A (zh) 2018-12-27 2021-11-09 生命编辑制药股份有限公司 可用于基因编辑的多肽及使用方法
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN109799295A (zh) * 2019-01-28 2019-05-24 江南大学 一种用于秀丽隐杆线虫代谢组学分析的样品前处理方法
US12460230B2 (en) 2019-02-10 2025-11-04 The J. David Gladstone Institutes Modified mitochondrion and methods of use thereof
MX2021009750A (es) 2019-02-12 2021-09-08 Univ Degli Studi Di Trento Moleculas del acido ribonucleico guia de cas12a y usos de las mismas.
EP3924483A4 (en) * 2019-02-13 2023-04-19 Beam Therapeutics, Inc. Splice acceptor site disruption of a disease-associated gene using adenosine deaminase base editors, including for the treatment of genetic disease
WO2020164702A1 (en) * 2019-02-13 2020-08-20 Probiogen Ag Transposase with enhanced insertion site selection properties
US20220235347A1 (en) 2019-02-13 2022-07-28 Beam Therapeutics Inc. Compositions and methods for treating hemoglobinopathies
JP7608348B2 (ja) * 2019-02-15 2025-01-06 アレン インスティテュート 選択されたニューロン細胞集団における遺伝子発現を選択的に調節するための人工発現コンストラクト
CN113811608B (zh) * 2019-02-22 2024-10-29 合成Dna技术公司 毛螺菌科细菌nd2006 cas12a突变型基因和由其编码的多肽
CN113748202B (zh) 2019-03-01 2024-07-16 艾欧凡斯生物治疗公司 由液体肿瘤扩增肿瘤浸润淋巴细胞及其治疗用途
CN109975543B (zh) * 2019-03-01 2022-02-11 中国疾病预防控制中心传染病预防控制所 分枝杆菌Ku蛋白的应用
WO2020181102A1 (en) * 2019-03-07 2020-09-10 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof
CN113728106A (zh) 2019-03-08 2021-11-30 齐默尔根公司 微生物中的迭代基因组编辑
US11053515B2 (en) 2019-03-08 2021-07-06 Zymergen Inc. Pooled genome editing in microbes
EP3938510A1 (en) 2019-03-11 2022-01-19 Sorrento Therapeutics, Inc. Improved process for integration of dna constructs using rna-guided endonucleases
WO2020186231A2 (en) 2019-03-14 2020-09-17 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
US12534714B2 (en) 2019-03-18 2026-01-27 The Broad Institute, Inc. Type VII CRISPR proteins and systems
WO2020190927A1 (en) 2019-03-18 2020-09-24 Regeneron Pharmaceuticals, Inc. Crispr/cas dropout screening platform to reveal genetic vulnerabilities associated with tau aggregation
ES2970541T3 (es) 2019-03-18 2024-05-29 Regeneron Pharma Plataforma de cribado CRISPR/CAS para identificar modificadores genéticos de la siembra o agregación de tau
WO2020191246A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
US10815467B2 (en) 2019-03-25 2020-10-27 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
CN113727603B (zh) 2019-04-03 2024-03-19 瑞泽恩制药公司 用于将抗体编码序列插入到安全港基因座中的方法和组合物
CN116200342A (zh) 2019-04-03 2023-06-02 精密生物科学公司 包含microRNA适应的shRNA（shRNAmiR）的遗传修饰的免疫细胞
IL286905B2 (en) 2019-04-04 2024-06-01 Regeneron Pharma Non-human animals containing the human coagulation factor 12 locus
RU2771374C1 (ru) 2019-04-04 2022-05-04 Редженерон Фармасьютикалс, Инк. Способы для бесшовного внесения целевых модификаций в направленные векторы
CA3136265A1 (en) 2019-04-05 2020-10-08 Precision Biosciences, Inc. Methods of preparing populations of genetically-modified immune cells
JP7426120B2 (ja) 2019-04-05 2024-02-01 国立大学法人大阪大学 ノックイン細胞の作製方法
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
EP3959223A4 (en) * 2019-04-25 2023-07-19 Flagship Pioneering Innovations VI, LLC COMPOSITIONS AND METHODS FOR PLANT KNIFE PACKS
CN110396543A (zh) * 2019-04-30 2019-11-01 广州普世利华科技有限公司 一种肿瘤相关基因突变位点筛查方法
CN110195102A (zh) * 2019-04-30 2019-09-03 广州普世利华科技有限公司 一种β地中海贫血基因分型方法
CA3138329A1 (en) 2019-05-10 2020-11-19 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
US20220249559A1 (en) 2019-05-13 2022-08-11 Iovance Biotherapeutics, Inc. Methods and compositions for selecting tumor infiltrating lymphocytes and uses of the same in immunotherapy
UY38693A (es) 2019-05-13 2020-12-31 Kws Saat Se & Co Kgaa Tolerancia a la sequía en maíz
CN114703142B (zh) * 2019-05-16 2024-05-10 上海苹谱医疗科技有限公司 一种人诱导多潜能干细胞、构建方法及其用途
WO2020236972A2 (en) * 2019-05-20 2020-11-26 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
CN114207137A (zh) 2019-05-21 2022-03-18 桑格摩生物治疗股份有限公司 调节性t细胞中受控的转基因表达
AU2020278906A1 (en) 2019-05-23 2021-11-25 Nomad Bioscience Gmbh RNA viral RNA molecule for gene editing
AR118995A1 (es) 2019-05-25 2021-11-17 Kws Saat Se & Co Kgaa Mejorador de la inducción de haploides
WO2020243164A1 (en) * 2019-05-28 2020-12-03 Octant, Inc. Transcriptional relay system
GB201907733D0 (en) * 2019-05-31 2019-07-17 Governing Council Of The Univ Of Toronto Methods and compositions for multiplex gene editing
KR20220016869A (ko) 2019-06-04 2022-02-10 리제너론 파마슈티칼스 인코포레이티드 베타-슬립 돌연변이를 갖는 인간화 ttr 좌위를 포함하는 비-인간 동물 및 사용 방법
AU2020288623A1 (en) 2019-06-06 2022-01-06 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
US11622547B2 (en) * 2019-06-07 2023-04-11 Regeneran Pharmaceuticals, Inc. Genetically modified mouse that expresses human albumin
PH12021553076A1 (en) 2019-06-07 2023-08-14 Scribe Therapeutics Inc Engineered casx systems
CA3134097A1 (en) 2019-06-13 2020-12-17 Wim VRIEZEN Tomato plant producing fruit having improved ripening characteristics
KR20220024053A (ko) 2019-06-14 2022-03-03 리제너론 파마슈티칼스 인코포레이티드 타우병증의 모델
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
CN114630910A (zh) 2019-06-25 2022-06-14 伊纳瑞农业技术有限公司 改善的同源依赖修复基因组编辑
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
EP3994270A1 (en) 2019-07-02 2022-05-11 Fred Hutchinson Cancer Research Center Recombinant ad35 vectors and related gene therapy improvements
CN112168808B (zh) * 2019-07-02 2023-01-24 深圳市第二人民医院 一种基于crispr的细胞核靶向药物递送系统
US12254959B2 (en) 2019-07-03 2025-03-18 Integrated Dna Technologies, Inc. Identification, characterization, and quantitation of CRISPR-introduced double-stranded DNA break repairs
AU2020309724A1 (en) 2019-07-05 2022-02-24 Centre National De La Recherche Scientifique Method for increasing yield in plants
MX2022000553A (es) * 2019-07-17 2022-04-25 Fate Therapeutics Inc Modificación de células efectoras inmunitarias y uso de las mismas.
US11746354B2 (en) 2019-07-19 2023-09-05 Inari Agriculture Technology, Inc. Homology dependent repair genome editing
EP4004216A1 (en) 2019-07-25 2022-06-01 Precision BioSciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
WO2021021532A1 (en) * 2019-07-26 2021-02-04 Mammoth Biosciences, Inc. Compositions for detection of dna and methods of use thereof
AU2020322021A1 (en) 2019-07-30 2022-02-10 Pairwise Plants Services, Inc. Morphogenic regulators and methods of using the same
CN110358767B (zh) * 2019-07-30 2021-06-08 湖北大学 一种基于CRISPR-Cas12a系统的运动发酵单胞菌基因组编辑方法及其应用
AU2020324386A1 (en) 2019-08-02 2022-02-24 Monsanto Technology Llc Methods and compositions to promote targeted genome modifications using HUH endonucleases
EP3772542A1 (en) 2019-08-07 2021-02-10 KWS SAAT SE & Co. KGaA Modifying genetic variation in crops by modulating the pachytene checkpoint protein 2
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
EP4013864A1 (en) 2019-08-12 2022-06-22 Lifeedit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
CN110592014A (zh) * 2019-08-14 2019-12-20 广东美赛尔细胞生物科技有限公司 一种在nk细胞治疗中免辐照体外体内持续去除饲养细胞的方法
US20230056843A1 (en) * 2019-08-19 2023-02-23 Southern Medical University Construction of high-fidelity crispr/ascpf1 mutant and uses thereof
WO2021035054A1 (en) 2019-08-20 2021-02-25 Precision Biosciences, Inc. Lymphodepletion dosing regimens for cellular immunotherapies
WO2021035170A1 (en) 2019-08-21 2021-02-25 Precision Biosciences, Inc. Compositions and methods for tcr reprogramming using fusion proteins
CN112442515B (zh) * 2019-09-02 2023-01-10 南京启真基因工程有限公司 gRNA靶点组合在构建血友病模型猪细胞系中的应用
WO2021046257A1 (en) 2019-09-03 2021-03-11 The Broad Institute, Inc. Crispr effector system based multiplex cancer diagnostics
AU2020341711A1 (en) * 2019-09-05 2022-03-03 Arbor Biotechnologies, Inc. Novel CRISPR DNA targeting enzymes and systems
CN114729381A (zh) * 2019-09-05 2022-07-08 本森希尔股份有限公司 修饰基因组的组合物和方法
EP4028047A4 (en) 2019-09-09 2023-07-26 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
JP2022547564A (ja) 2019-09-10 2022-11-14 サイエンス ソリューションズ エル・エル・シー 新規なクラス２のｉｉ型及びｖ型ｃｒｉｓｐｒ－ｃａｓ ｒｎａ誘導型エンドヌクレアーゼ
CN110628816B (zh) * 2019-09-12 2022-10-18 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种用于血友病a的双切供体及其药物组合
AU2020344905A1 (en) 2019-09-12 2022-04-28 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
KR20220062079A (ko) 2019-09-13 2022-05-13 리제너론 파마슈티칼스 인코포레이티드 지질 나노입자에 의해 전달되는 CRISPR/Cas 시스템을 사용한 동물에서의 전사 조절
CN110600083B (zh) * 2019-09-20 2020-05-19 中国人民解放军军事科学院军事医学研究院 基于无拼接组装wgs数据的醋酸钙—鲍曼不动杆菌复合群鉴定方法
CA3147641A1 (en) 2019-09-23 2021-04-01 Omega Therapeutics, Inc. Compositions and methods for modulating apolipoprotein b (apob) gene expression
AU2020352552A1 (en) 2019-09-23 2022-03-17 Omega Therapeutics, Inc. Compositions and methods for modulating hepatocyte nuclear factor 4-alpha (HNF4α) gene expression
CA3153197A1 (en) * 2019-10-03 2021-04-08 Ryan T. Gill Crispr systems with engineered dual guide nucleic acids
WO2021069387A1 (en) 2019-10-07 2021-04-15 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
WO2021072328A1 (en) 2019-10-10 2021-04-15 The Broad Institute, Inc. Methods and compositions for prime editing rna
AU2020366566A1 (en) 2019-10-17 2022-04-21 KWS SAAT SE & Co. KGaA Enhanced disease resistance of crops by downregulation of repressor genes
WO2021076682A1 (en) * 2019-10-17 2021-04-22 Pairwise Plants Services, Inc. Variants of cas12a nucleases and methods of making and use thereof
US12385053B2 (en) 2019-10-22 2025-08-12 Inari Agriculture Technology, Inc. Genomic alteration of plant germline
AU2020369599A1 (en) 2019-10-23 2022-05-12 Monsanto Technology Llc Compositions and methods for RNA-templated editing in plants
WO2021081384A1 (en) * 2019-10-25 2021-04-29 Greenvenus, Llc Synthetic nucleases
EP4048295A1 (en) 2019-10-25 2022-08-31 Iovance Biotherapeutics, Inc. Gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
WO2021087182A1 (en) 2019-10-30 2021-05-06 Pairwise Plants Services, Inc. Type v crispr-cas base editors and methods of use thereof
US20220411479A1 (en) 2019-10-30 2022-12-29 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
US20230002746A1 (en) * 2019-10-31 2023-01-05 Inari Agriculture Technology, Inc. Base-editing systems
MX2022005328A (es) 2019-11-05 2022-07-21 Pairwise Plants Services Inc Composiciones y metodos para el reemplazo de alelos con adn codificado por arn.
CN114746125B (zh) 2019-11-08 2025-08-08 瑞泽恩制药公司 用于x连锁青少年型视网膜劈裂症疗法的crispr和aav策略
WO2021098709A1 (zh) * 2019-11-18 2021-05-27 中国科学院遗传与发育生物学研究所 衍生自黄杆菌的基因编辑系统
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
CN110760597B (zh) * 2019-11-22 2022-08-23 西北农林科技大学 一种检测黄牛ncstn基因拷贝数变异的方法及其应用
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
WO2021108501A1 (en) * 2019-11-27 2021-06-03 The General Hospital Corporation System and method for activating gene expression
EP4065701A4 (en) 2019-11-27 2023-11-29 Danmarks Tekniske Universitet CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY
WO2021105191A1 (en) 2019-11-29 2021-06-03 Basf Se Increasing resistance against fungal infections in plants
CN114787365A (zh) 2019-12-03 2022-07-22 巴斯夫欧洲公司 用于在植物中增强基因表达的调节性核酸分子
JP2023505234A (ja) * 2019-12-04 2023-02-08 アーバー バイオテクノロジーズ， インコーポレイテッド ヌクレアーゼを含む組成物及びその使用
WO2021113788A1 (en) * 2019-12-06 2021-06-10 Pairwise Plants Services, Inc. Recruitment methods and compounds, compositions and systems for recruitment
US20230036065A1 (en) 2019-12-06 2023-02-02 Precision Biosciences, Inc. Methods for cancer immunotherapy
AU2020402526B2 (en) 2019-12-10 2025-01-23 Inscripta, Inc. Novel MAD nucleases
JP7749561B2 (ja) 2019-12-11 2025-10-06 アイオバンス バイオセラピューティクス，インコーポレイテッド 腫瘍浸潤リンパ球（ｔｉｌ）の産生のためのプロセス及びそれを使用する方法
CN110791523B (zh) * 2019-12-13 2022-05-10 南京农业大学 一种棉花抗旱相关基因GhRCHY1及其应用
KR102493904B1 (ko) * 2019-12-13 2023-01-31 한국생명공학연구원 EeCpf1에 의해 IL2Rg 유전자가 돌연변이된 면역부전 동물모델 및 그 제조방법
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
WO2021122081A1 (en) 2019-12-16 2021-06-24 Basf Se Precise introduction of dna or mutations into the genome of wheat
CN114829612A (zh) 2019-12-16 2022-07-29 巴斯夫农业种子解决方案美国有限责任公司 使用配对切口酶的改进的基因组编辑
CN114829607A (zh) 2019-12-18 2022-07-29 因思科瑞普特公司 用于体内检测核酸引导的核酸酶编辑的细胞的Cascade/dCas3互补测定
KR20220116504A (ko) 2019-12-19 2022-08-23 바스프 에스이 정밀 화학물의 제조에서 공시 수율, 탄소-전환-효율 및 탄소 기질 적응성의 증가
JP2023508871A (ja) 2019-12-20 2023-03-06 ビーエーエスエフ ソシエタス・ヨーロピア テルペンの毒性の低減および微生物中での生成可能性の増大
EP4085133A1 (en) 2019-12-30 2022-11-09 Lifeedit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
CN111073902B (zh) * 2019-12-30 2022-04-26 广东省微生物研究所（广东省微生物分析检测中心） 提升胶霉毒素生物合成基因表达水平的CRISPR/dCas9载体及其构建方法和应用
CN113073118B (zh) * 2020-01-03 2023-09-15 珠海联邦制药股份有限公司 Tet-off表达调控系统在级联放大启动子活性中的用途
MX2022008359A (es) 2020-01-06 2022-10-07 Pairwise Plants Services Inc Reclutamiento de adn polimerasa para edicion con molde.
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
WO2021146641A1 (en) 2020-01-17 2021-07-22 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
CA3168604A1 (en) 2020-01-21 2021-07-29 Limagrain Europe Wheat haploid inducer plant and uses
US12264341B2 (en) 2020-01-24 2025-04-01 The General Hospital Corporation CRISPR-Cas enzymes with enhanced on-target activity
US12312613B2 (en) 2020-01-24 2025-05-27 The General Hospital Corporation Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
EP4093907A4 (en) * 2020-01-24 2024-01-17 The General Hospital Corporation METHODS FOR CHARACTERIZING ENZYMES FOR GENOMICS ENGINEERING
EP4096770A1 (en) 2020-01-27 2022-12-07 Inscripta, Inc. Electroporation modules and instrumentation
AU2021212189A1 (en) 2020-01-30 2022-08-04 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
AR121242A1 (es) 2020-01-31 2022-05-04 Pairwise Plants Services Inc Supresión de la respuesta de escape a la sombra en plantas
US20230063560A1 (en) 2020-02-04 2023-03-02 Pairwise Plans Services, Inc. Thornless and/or prickleless rubus plants
CN111337666B (zh) * 2020-02-12 2021-04-02 山东大学 I-motif重组介导的FRET探针及其原位成像癌细胞表面蛋白质同源二聚化的应用
CN113261532A (zh) * 2020-02-17 2021-08-17 中山大学 一种肺癌动物模型的构建方法
EP4107273A1 (en) 2020-02-21 2022-12-28 Limagrain Europe Prime editing technology for plant genome engineering
WO2021168288A1 (en) 2020-02-21 2021-08-26 Pairwise Plants Services, Inc. Improved resistance to soybean cyst nematode through gene editing
JP2023515573A (ja) 2020-02-26 2023-04-13 ソレント・セラピューティクス・インコーポレイテッド 普遍的な遮蔽性部分を有する活性化可能な抗原結合性タンパク質
US12012433B1 (en) * 2020-02-27 2024-06-18 Integrated Dna Technologies Inc. Expression and purification of Cas enzymes
EP4110929A1 (en) 2020-02-28 2023-01-04 KWS SAAT SE & Co. KGaA Immature inflorescence meristem editing
CN115768898A (zh) 2020-02-28 2023-03-07 科沃施种子欧洲股份两合公司 用于顽拗型植物的快速基因组修饰的方法
BR112022017070A2 (pt) * 2020-02-28 2022-11-16 Huigene Therapeutics Co Ltd Sistema crispr-cas tipo vi-e e tipo vi-f e usos do mesmo
CN115485385A (zh) 2020-03-04 2022-12-16 瑞泽恩制药公司 用于使肿瘤细胞对免疫疗法敏感的方法和组合物
EP3878958A1 (en) 2020-03-11 2021-09-15 B.R.A.I.N. Biotechnology Research And Information Network AG Crispr-cas nucleases from cpr-enriched metagenome
AU2021234302A1 (en) 2020-03-11 2022-11-10 Omega Therapeutics, Inc. Compositions and methods for modulating forkhead box p3 (foxp3) gene expression
US20220228150A1 (en) 2020-03-17 2022-07-21 Gowtham THAKKU Crispr system high throughput diagnostic systems and methods
EP4125348A1 (en) 2020-03-23 2023-02-08 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
US11999946B2 (en) 2020-03-26 2024-06-04 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US11882808B2 (en) 2020-03-27 2024-01-30 Pairwise Plants Services, Inc. Methods for improving resistance to soybean rust
WO2021202513A1 (en) 2020-03-31 2021-10-07 Elo Life Systems Modulation of endogenous mogroside pathway genes in watermelon and other cucurbits
WO2021207148A1 (en) 2020-04-06 2021-10-14 Pairwise Plants Services, Inc. Methods and compositions for increasing resistance to ear rot and stem rot disease in maize
US20230340538A1 (en) 2020-04-08 2023-10-26 Astrazeneca Ab Compositions and methods for improved site-specific modification
CA3180157A1 (en) 2020-04-16 2021-10-21 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
EP4139345A1 (en) 2020-04-24 2023-03-01 Sorrento Therapeutics, Inc. Memory dimeric antigen receptors
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
TW202208626A (zh) 2020-04-24 2022-03-01 美商生命編輯公司 Rna引導核酸酶及其活性片段與變體，以及使用方法
JP2023524066A (ja) 2020-05-01 2023-06-08 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド 非カノニカルｔｔｔｔプロトスペーサー隣接モチーフにて増強された切断活性を備えるラクノスピラセｓｐ．のｃａｓ１２ａ変異体
EP4146794A1 (en) 2020-05-04 2023-03-15 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
GB2614813B (en) 2020-05-08 2025-05-07 Harvard College Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
WO2021224506A1 (en) * 2020-05-08 2021-11-11 Universitat Pompeu Fabra Crispr-cas homology directed repair enhancer
US20230183664A1 (en) 2020-05-11 2023-06-15 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
CA3178193A1 (en) 2020-05-13 2021-11-18 Wim VRIEZEN Method for obtaining mutant plants by targeted mutagenesis
MX2022014185A (es) 2020-05-13 2022-12-07 Nunhems Bv Plantas de tomate con recombinacion meiotica suprimida.
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
US20230175019A1 (en) * 2020-05-28 2023-06-08 University Of Southern California Scalable trio guide rna approach for integration of large donor dna
UY39237A (es) 2020-05-29 2021-12-31 Kws Saat Se & Co Kgaa Inducción de haploides en plantas
MX2022015107A (es) 2020-06-02 2023-03-01 Pairwise Plants Services Inc Metodos para controlar el tama?o del meristema para mejorar los cultivos.
CA3178670A1 (en) * 2020-06-03 2021-12-09 Lucas Benjamin HARRINGTON Programmable nucleases and methods of use
CN111808832B (zh) * 2020-06-05 2022-02-11 华南农业大学 一种水稻纹枯病菌阳离子转运ATP酶基因及其片段Rscta和应用
JP7419168B2 (ja) * 2020-06-10 2024-01-22 株式会社東芝 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法
TW202219272A (zh) 2020-06-12 2022-05-16 美商夏洛克生物科學公司 SARS-CoV-2之偵測
UY39278A (es) 2020-06-17 2022-01-31 Pairwise Plants Services Inc Métodos para el control del tamaño del meristemo para la mejora de cultivos
US20240216508A1 (en) 2020-06-26 2024-07-04 Juno Therapeutics Gmbh Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
WO2022002989A1 (en) 2020-06-29 2022-01-06 KWS SAAT SE & Co. KGaA Boosting homology directed repair in plants
WO2022006226A1 (en) 2020-06-30 2022-01-06 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
IL299812A (en) 2020-07-15 2023-03-01 Lifeedit Therapeutics Inc Uracil stabilizing proteins and active fragments and their variants and methods of use
CN111778252B (zh) * 2020-07-17 2022-01-07 湖北省农业科学院畜牧兽医研究所 一种靶向敲除SST基因的sgRNA及其CRISPR/Cas9系统和应用
EP3943600A1 (en) 2020-07-21 2022-01-26 B.R.A.I.N. Biotechnology Research And Information Network AG Novel, non-naturally occurring crispr-cas nucleases for genome editing
AU2021313163B2 (en) 2020-07-21 2025-11-27 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
WO2022032195A2 (en) * 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Spatial sequencing
WO2022035793A1 (en) 2020-08-10 2022-02-17 Precision Biosciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
CA3188988A1 (en) * 2020-08-13 2022-02-17 Sidi CHEN Compositions and methods for engineering and selection of car t cells with desired phenotypes
CN111979273B (zh) * 2020-08-24 2022-05-27 苏州启辰生物科技有限公司 一种制备人源化ace2小鼠模型的方法
EP4199956A2 (en) * 2020-08-24 2023-06-28 Crisp-HR Therapeutics, Inc. A nuclease and application thereof
JP2023539668A (ja) 2020-08-28 2023-09-15 ペアワイズ・プランツ・サーヴィシズ，インコーポレイテッド 操作されたＣＲＩＳＰＲ－Ｃａｓタンパク質およびその使用方法
EP4208547A1 (en) 2020-09-01 2023-07-12 64-X, Inc. Mammalian cells and methods for engineering the same
BR112023003784A2 (pt) 2020-09-01 2023-03-28 Univ Leland Stanford Junior Sistema crispr-cas (casmini) em miniatura sintético para engenharia de genoma eucariótico
AU2021336681A1 (en) 2020-09-02 2023-03-30 Massachusetts Institute Of Technology Systems, methods, and compositions for RNA-guided RNA-targeting CRISPR effectors
JP7719172B2 (ja) 2020-09-11 2025-08-05 ライフ エディット セラピューティクス，インコーポレイティド Ｄｎａ修飾酵素並びにその活性断片及びバリアント並びにその使用方法
US11299731B1 (en) 2020-09-15 2022-04-12 Inscripta, Inc. CRISPR editing to embed nucleic acid landing pads into genomes of live cells
AU2021343526A1 (en) 2020-09-18 2023-05-25 Celyntra Therapeutics Sa Constructs and uses thereof for efficient and specific genome editing
WO2022068912A1 (en) 2020-09-30 2022-04-07 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022188039A1 (en) 2021-03-09 2022-09-15 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022076606A1 (en) 2020-10-06 2022-04-14 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
US20230372397A1 (en) 2020-10-06 2023-11-23 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
WO2022076547A1 (en) 2020-10-07 2022-04-14 Precision Biosciences, Inc. Lipid nanoparticle compositions
CR20230175A (es) 2020-10-23 2023-07-26 Elo Life Systems Inc Métodos para producir plantas de vainilla con mejor sabor y producción agronómica
US20240026369A1 (en) 2020-10-27 2024-01-25 KWS SAAT SE & Co. KGaA Use of enhanced pol theta activity for eukaryotic genome engineering
CN112300999A (zh) * 2020-11-02 2021-02-02 江苏集萃药康生物科技股份有限公司 一种balb/c小鼠模型及其用途
EP4240853A2 (en) * 2020-11-03 2023-09-13 Science Solutions LLC Novel class 2 crispr-cas rna-guided endonucleases
MX2023005150A (es) 2020-11-06 2023-05-26 Pairwise Plants Services Inc Composiciones y metodos para el reemplazo de alelos con adn codificado por arn.
WO2022097663A1 (ja) 2020-11-06 2022-05-12 エディットフォース株式会社 ＦｏｋＩヌクレアーゼドメインの変異体
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
EP4243608A1 (en) 2020-11-11 2023-09-20 Leibniz-Institut für Pflanzenbiochemie (IPB) Fusion protein for editing endogenous dna of a eukaryotic cell
AU2021377686A1 (en) * 2020-11-11 2023-06-22 The Trustees Of Columbia University In The City Of New York Multiplex epigenome editing
CN112410355B (zh) * 2020-11-23 2022-03-25 昆明理工大学 一种酰基辅酶a氧化酶2基因rkacox2及其应用
US20230416765A1 (en) 2020-11-23 2023-12-28 Pairwise Plants Services, Inc Agrobacterium rhizogenes and methods of transforming cells
CN112461807B (zh) * 2020-11-26 2021-11-19 山西大学 碳量子点在靶向细胞核仁免洗成像中的应用
US20240002839A1 (en) 2020-12-02 2024-01-04 Decibel Therapeutics, Inc. Crispr sam biosensor cell lines and methods of use thereof
TW202237836A (zh) 2020-12-03 2022-10-01 美商斯奎柏治療公司 工程化之第2類第v型crispr系統
CN112195164B (zh) * 2020-12-07 2021-04-23 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN114634928B (zh) * 2020-12-15 2023-08-04 四川大学华西医院 一种降低stat3转录功能的核酸片段及其制药用途
JP2023554395A (ja) 2020-12-17 2023-12-27 アイオバンス バイオセラピューティクス，インコーポレイテッド Ｃｔｌａ－４及びｐｄ－１阻害剤と併用した腫瘍浸潤リンパ球療法による治療
WO2022133149A1 (en) 2020-12-17 2022-06-23 Iovance Biotherapeutics, Inc. Treatment of cancers with tumor infiltrating lymphocytes
CA3205601A1 (en) * 2020-12-17 2022-06-23 Monsanto Technology Llc Engineered ssdnase-free crispr endonucleases
EP4019639A1 (en) 2020-12-22 2022-06-29 KWS SAAT SE & Co. KGaA Promoting regeneration and transformation in beta vulgaris
EP4267157A4 (en) 2020-12-23 2025-05-07 Flagship Pioneering Innovations V, Inc. IN VITRO ASSEMBLY OF ANELLOVIRUS CAPSIDS WITH RNA
CN112553215A (zh) * 2020-12-24 2021-03-26 山东农业大学 一个玉米纹枯病抗病相关基因ZmFAH49及其应用
CN112662670B (zh) * 2020-12-25 2024-04-02 河南省农业科学院 花生脂酰-酰基载体蛋白硫酯酶AhFATB2基因启动子及其制备方法和应用
CN112640847B (zh) * 2020-12-30 2022-12-13 重庆医科大学附属第一医院 一种内源性癫痫发作动物模型及其构建方法
WO2022146497A1 (en) 2021-01-04 2022-07-07 Inscripta, Inc. Mad nucleases
US11332742B1 (en) 2021-01-07 2022-05-17 Inscripta, Inc. Mad nucleases
CA3207102A1 (en) 2021-01-12 2022-07-21 March Therapeutics, Inc. Context-dependent, double-stranded dna-specific deaminases and uses thereof
CN112680479A (zh) * 2021-01-13 2021-04-20 汪利平 Cyp1b1基因缺失斑马鱼的制备
CN114790456A (zh) * 2021-01-24 2022-07-26 东北林业大学 野菊u6基因及其应用
US20240084332A1 (en) * 2021-01-25 2024-03-14 The Broad Institute, Inc. Reprogrammable tnpb polypeptides and use thereof
US20250127811A1 (en) 2021-01-28 2025-04-24 Precision Biosciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
CA3206549A1 (en) 2021-01-29 2022-08-04 Frederick G. Vogt Methods of making modified tumor infiltrating lymphocytes and their use in adoptive cell therapy
CN112877275B (zh) * 2021-02-04 2023-03-31 中国农业科学院兰州兽医研究所 Hdac2基因敲除的bhk-21细胞系及其构建方法和应用
CN112852745B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac3基因敲除的bhk-21细胞系及其构建方法和应用
CN114517190B (zh) * 2021-02-05 2022-12-23 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
KR20230142776A (ko) 2021-02-07 2023-10-11 후이다진 테라퓨틱스 (싱가포르) 피티이. 엘티디. Rna 아데노 관련 바이러스(raav) 벡터 및 그 용도
EP4291678A1 (en) 2021-02-09 2023-12-20 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
EP4291641A1 (en) 2021-02-11 2023-12-20 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin oxidase levels in plants
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
WO2022175878A1 (en) * 2021-02-20 2022-08-25 Kcat Enzymatic Private Limited Insilico guided crispr-cas driven enzyme engineering framework
WO2022182834A1 (en) 2021-02-25 2022-09-01 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
AU2022227650A1 (en) 2021-02-25 2023-10-12 Celyntra Therapeutics Sa Compositions and methods for targeting, editing, or modifying genes
EP4301852A1 (en) 2021-03-02 2024-01-10 BRAIN Biotech AG Novel crispr-cas nucleases from metagenomes
CN117693508A (zh) 2021-03-03 2024-03-12 朱诺治疗学股份有限公司 T细胞疗法和dgk抑制剂的组合
EP4305157A1 (en) 2021-03-09 2024-01-17 Huidagene Therapeutics (Singapore) Pte. Ltd. Engineered crispr/cas13 system and uses thereof
EP4305159A4 (en) * 2021-03-09 2025-04-16 Arbor Biotechnologies, Inc. COMPOSITIONS COMPRISING A VARIANT POLYPEPTIDE AND USES THEREOF
CN113005147A (zh) * 2021-03-11 2021-06-22 中山大学附属第一医院 一种usp8突变的小鼠动物模型的构建方法及其应用
EP4308691A1 (en) 2021-03-19 2024-01-24 Iovance Biotherapeutics, Inc. Methods for tumor infiltrating lymphocyte (til) expansion related to cd39/cd69 selection and gene knockout in tils
WO2022204093A1 (en) 2021-03-22 2022-09-29 LifeEDIT Therapeutics, Inc. Dna modifyng enzymes and active fragments and variants thereof and methods of use
EP4320234A2 (en) 2021-04-07 2024-02-14 Astrazeneca AB Compositions and methods for site-specific modification
US20240200059A1 (en) 2021-04-09 2024-06-20 Vor Biopharma Inc. Photocleavable guide rnas and methods of use thereof
JP2024514649A (ja) 2021-04-16 2024-04-02 ビーム セラピューティクス インク． 肝細胞の遺伝子修飾
EP4326287A2 (en) 2021-04-19 2024-02-28 Iovance Biotherapeutics, Inc. Chimeric costimulatory receptors, chemokine receptors, and the use of same in cellular immunotherapies
KR102428773B1 (ko) * 2021-04-21 2022-08-05 (주)지플러스생명과학 분리형 crRNA 및 이를 이용한 CRISPR-Cas 시스템
WO2022226316A1 (en) 2021-04-22 2022-10-27 Precision Biosciences, Inc. Compositions and methods for generating male sterile plants
JP2024518413A (ja) 2021-05-06 2024-05-01 アーティザン ディヴェロップメント ラブス インコーポレイテッド 修飾ヌクレアーゼ
US20240269180A1 (en) 2021-05-17 2024-08-15 Iovance Biotherapeutics, Inc. Pd-1 gene-edited tumor infiltrating lymphocytes and uses of same in immunotherapy
WO2022243437A1 (en) 2021-05-19 2022-11-24 KWS SAAT SE & Co. KGaA Sample preparation with oppositely oriented guide polynucleotides
CN113287569B (zh) * 2021-05-27 2023-04-07 四川康城生物科技有限公司 一种免疫低下动物模型的构建方法
EP4419672A2 (en) 2021-06-01 2024-08-28 Artisan Development Labs, Inc. Compositions and methods for targeting, editing, or modifying genes
US20220389418A1 (en) * 2021-06-01 2022-12-08 Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) Rna-guided cas nucleases and uses thereof in diagnostics and therapy
BR112023024985A2 (pt) 2021-06-01 2024-02-20 Arbor Biotechnologies Inc Sistemas de edição de genes compreendendo uma crispr nuclease e usos dos mesmos
CN113064220A (zh) * 2021-06-03 2021-07-02 四川九通智路科技有限公司 基于非线性自回归神经网络的能见度测量系统及测量方法
AU2022290278A1 (en) 2021-06-11 2024-01-04 LifeEDIT Therapeutics, Inc. Rna polymerase iii promoters and methods of use
WO2022265905A2 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
WO2022266439A2 (en) * 2021-06-17 2022-12-22 The Board Of Trustees Of The University Of Illinois Crispr cascade
CA3223995A1 (en) 2021-06-17 2022-12-22 Pairwise Plants Services, Inc. Modification of growth regulating factor family transcription factors in soybean
CA3223311A1 (en) 2021-06-18 2022-12-22 Andrea BARGHETTI Compositions and methods for targeting, editing or modifying human genes
UY39827A (es) 2021-06-24 2023-01-31 Pairwise Plants Services Inc Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento
CA3224982A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
AU2022307018A1 (en) * 2021-07-05 2024-02-15 Genkore Inc. Cleavage-inactive cas12f1, cleavage-inactive cas12f1-based fusion protein, crispr gene-editing system comprising same, and preparation method and use thereof
CN117987554A (zh) * 2021-07-06 2024-05-07 苏州淦江生物技术有限公司 一种基于Cas蛋白的检测方法
WO2023283585A2 (en) 2021-07-06 2023-01-12 Vor Biopharma Inc. Inhibitor oligonucleotides and methods of use thereof
WO2023283359A2 (en) 2021-07-07 2023-01-12 Omega Therapeutics, Inc. Compositions and methods for modulating secreted frizzled receptor protein 1 (sfrp1) gene expression
CN113383747B (zh) * 2021-07-09 2022-12-27 广西贺州农贝贝农牧科技有限公司 一种产蛋后期老龄鸡强制换羽的工艺方法
WO2023283636A1 (en) * 2021-07-09 2023-01-12 Acrigen Biosciences Compositions and methods for nucleic acid modifications
WO2023287669A2 (en) 2021-07-12 2023-01-19 Labsimply, Inc. Nuclease cascade assay
WO2023004074A2 (en) 2021-07-22 2023-01-26 Iovance Biotherapeutics, Inc. Method for cryopreservation of solid tumor fragments
CA3226942A1 (en) 2021-07-28 2023-02-02 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with kras inhibitors
AR126622A1 (es) 2021-07-30 2023-10-25 Kws Saat Se & Co Kgaa Plantas con digestibilidad mejorada y haplotipos marcadores
CA3228272A1 (en) 2021-08-02 2023-02-09 Vor Biopharma Inc. Compositions and methods for gene modification
MX2024001883A (es) 2021-08-12 2024-02-29 Pairwise Plants Services Inc Modificacion de los genes receptores de brasinoesteroides para mejorar los rasgos de rendimiento.
WO2023020938A1 (en) 2021-08-16 2023-02-23 Nunhems B.V. Lettuce plant having delayed bolting
UY39902A (es) 2021-08-17 2023-03-31 Pairwise Plants Services Inc Métodos y composiciones para modificar genes de histidina quinasa receptores de citoquinina en plant
WO2023028444A1 (en) * 2021-08-27 2023-03-02 Mammoth Biosciences, Inc. Effector proteins and methods of use
EP4395531A1 (en) 2021-08-30 2024-07-10 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
CN113584077B (zh) * 2021-08-31 2023-09-15 西南大学 沉默桑实杯盘菌关键致病基因CsGPA1的沉默载体及其应用和方法
AR126938A1 (es) 2021-09-02 2023-11-29 Pairwise Plants Services Inc Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento
CN118382634A (zh) 2021-09-21 2024-07-23 成对植物服务股份有限公司 用于减少油菜荚果开裂的方法和组合物
WO2023049728A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
EP4409007A4 (en) 2021-09-27 2025-10-08 Monsanto Technology Llc COMPOSITIONS AND METHODS FOR TRANSFORMING EXPLANTS OF EXCISED EMBRYO FROM MONOCOTYLEDONOUS SEEDS
US20240417755A1 (en) 2021-09-27 2024-12-19 Vor Biopharma Inc. Fusion polypeptides for genetic editing and methods of use thereof
JP2024536135A (ja) 2021-09-30 2024-10-04 アストラゼネカ・アクチエボラーグ ＣＲＩＳＰＲ／Ｃａｓ挿入の効率を増大させるための阻害剤の使用
CA3237641A1 (en) 2021-10-04 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023059115A1 (ko) * 2021-10-06 2023-04-13 주식회사 진코어 유전자 편집을 위한 target 시스템 및 이의 용도
CA3234455A1 (en) 2021-10-07 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023064872A1 (en) 2021-10-14 2023-04-20 Precision Biosciences, Inc. Combinations of anti-bcma car t cells and gamma secretase inhibitors
EP4166670A1 (en) 2021-10-18 2023-04-19 KWS SAAT SE & Co. KGaA Plant-tag-based weeding control
US20240408235A1 (en) 2021-10-19 2024-12-12 Precision Biosciences, Inc. Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency
CN116024254A (zh) * 2021-10-26 2023-04-28 成都极谷基因科技有限公司 编辑水稻基因组中OsGBSSⅠ启动子及内含子1以提高稻米品质的方法
WO2023077015A2 (en) 2021-10-27 2023-05-04 Iovance Biotherapeutics, Inc. Systems and methods for coordinating manufacturing of cells for patient-specific immunotherapy
JP2024540086A (ja) 2021-10-28 2024-10-31 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｃ５をノックアウトするためのｃｒｉｓｐｒ／ｃａｓ関連方法及び組成物
KR20240099393A (ko) 2021-11-01 2024-06-28 톰 바이오사이언시스, 인코포레이티드 유전자 편집 기구와 핵산 카고의 동시 전달을 위한 단일 작제물 플랫폼
WO2023081900A1 (en) 2021-11-08 2023-05-11 Juno Therapeutics, Inc. Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods
EP4430167A1 (en) 2021-11-10 2024-09-18 Iovance Biotherapeutics, Inc. Methods of expansion treatment utilizing cd8 tumor infiltrating lymphocytes
US20250009800A1 (en) 2021-11-16 2025-01-09 Precision Biosciences, Inc. Methods for cancer immunotherapy
WO2023097316A1 (en) * 2021-11-29 2023-06-01 Editas Medicine, Inc. Engineered crispr/cas12a effector proteins, and uses thereof
CN114404429B (zh) * 2021-11-30 2023-06-30 重庆医科大学附属第二医院 一种纳米银修饰的单宁酸-铁网络的载药纳米复合物及其制备方法和逆转肿瘤耐药应用
CN118632622A (zh) 2021-12-08 2024-09-10 瑞泽恩制药公司 突变型肌纤蛋白疾病模型及其用途
UY40060A (es) 2021-12-09 2023-07-14 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
WO2023114090A2 (en) 2021-12-13 2023-06-22 Labsimply, Inc. Signal boost cascade assay
US20230295646A1 (en) 2021-12-13 2023-09-21 Pairwise Plants Services, Inc. Model editing systems and methods relating to the same
WO2023114052A1 (en) 2021-12-13 2023-06-22 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
US20250304982A1 (en) 2021-12-21 2025-10-02 Benson Hill, Inc. Compositions and methods for modifying genomes
WO2023122764A1 (en) 2021-12-22 2023-06-29 Tome Biosciences, Inc. Co-delivery of a gene editor construct and a donor template
EP4453212A1 (en) 2021-12-23 2024-10-30 University of Massachusetts Therapeutic treatment for fragile x-associated disorder
CA3246441A1 (en) 2022-01-06 2023-07-13 Pairwise Plants Services, Inc. METHODS AND COMPOSITIONS FOR THE ELIMINATION OF TRICHOMES
JP2025505063A (ja) * 2022-01-12 2025-02-20 ジーンシーカー カンパニー， リミテッド 増強された特異性を有するｃａｓ９タンパク質及びその使用
WO2023140695A1 (ko) * 2022-01-21 2023-07-27 한국과학기술연구원 우유 유래 엑소좀을 유효성분으로 포함하는 장 건강기능 개선용 조성물 및 그 제조방법
IL314488A (en) 2022-01-24 2024-09-01 Lifeedit Therapeutics Inc RNA-directed nucleases and active fragments and their variants and methods of use
WO2023147488A1 (en) 2022-01-28 2023-08-03 Iovance Biotherapeutics, Inc. Cytokine associated tumor infiltrating lymphocytes compositions and methods
AR128372A1 (es) 2022-01-31 2024-04-24 Pairwise Plants Services Inc Supresión de la respuesta de evitación de la sombra en las plantas
IL314482A (en) 2022-02-02 2024-09-01 Regeneron Pharma Anti-tfr:gaa and anti-cd63:gaa insertions for treatment of pompe disease
CN118843637A (zh) 2022-02-11 2024-10-25 东北农业大学 用于增加植物中蛋白质和/或油含量和改变油特性的方法和组合物
JP2025508723A (ja) * 2022-02-18 2025-04-10 プルモバイオティクス，エス．エル． 細菌を操作するためのシステム、ツール、及び方法
WO2023164722A1 (en) 2022-02-28 2023-08-31 Pairwise Plants Services, Inc. Engineered crispr-cas effector proteins and methods of use thereof
CN119137268A (zh) 2022-03-01 2024-12-13 埃皮克里斯皮尔生物技术公司 工程化核酸酶、组合物及其使用方法
EP4486881A1 (en) 2022-03-01 2025-01-08 Celyntra Therapeutics SA Composition and methods for transgene insertion
MX2024010647A (es) 2022-03-02 2024-09-06 Pairwise Plants Services Inc Modificacion de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento.
CN114271237B (zh) * 2022-03-07 2022-05-27 南京农业大学 一种长短绒自别雌雄鸽的培育方法
CN120944860A (zh) 2022-03-08 2025-11-14 中国科学院遗传与发育生物学研究所 胞嘧啶脱氨酶及其在碱基编辑中的用途
EP4499842A1 (en) 2022-03-31 2025-02-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
CN114592023B (zh) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 一种细胞裂解自组装多肽复合物、自组装方法、自组装多肽制剂及应用
CA3247638A1 (en) 2022-04-06 2023-10-12 Maria Fardis TREATMENT OF PATIENTS WITH NON-SMALL CELL LUNG CARCINOMA (NSCLC) WITH TUMOR-INFILTRATING LYMPHOCYTE THERAPIES
US20230357789A1 (en) 2022-04-07 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for improving resistance to fusarium head blight
EP4507704A1 (en) 2022-04-15 2025-02-19 Iovance Biotherapeutics, Inc. Til expansion processes using specific cytokine combinations and/or akti treatment
WO2023205744A1 (en) 2022-04-20 2023-10-26 Tome Biosciences, Inc. Programmable gene insertion compositions
UY40232A (es) 2022-04-21 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para mejorar rasgos de rendimiento
WO2023212677A2 (en) 2022-04-29 2023-11-02 Regeneron Pharmaceuticals, Inc. Identification of tissue-specific extragenic safe harbors for gene therapy approaches
UY40250A (es) 2022-05-02 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para mejorar el rendimiento y la resistencia a enfermedades
WO2023215831A1 (en) 2022-05-04 2023-11-09 Tome Biosciences, Inc. Guide rna compositions for programmable gene insertion
UY40255A (es) 2022-05-05 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectur radicular y/o mejorar los rangos de rendimient
CA3256953A1 (en) 2022-05-09 2023-11-16 Regeneron Pharmaceuticals, Inc. VECTORS AND METHODS FOR IN VIVO ANTIBODY PRODUCTION
EP4522202A1 (en) 2022-05-10 2025-03-19 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with an il-15r agonist
US20250308637A1 (en) 2022-05-20 2025-10-02 Celyntra Therapeutics Sa Systems and methods for assessing risk of genome editing events
WO2023225670A2 (en) 2022-05-20 2023-11-23 Tome Biosciences, Inc. Ex vivo programmable gene insertion
JP2025519096A (ja) 2022-05-25 2025-06-24 フラッグシップ パイオニアリング イノベーションズ セブン，エルエルシー 遺伝的ドライバーを調節するための組成物及び方法
JP2025517968A (ja) 2022-05-25 2025-06-12 フラッグシップ パイオニアリング イノベーションズ セブン，エルエルシー 免疫応答を調節するための組成物及び方法
KR20250019772A (ko) 2022-05-25 2025-02-11 플래그쉽 파이어니어링 이노베이션스 Vii, 엘엘씨 종양 억제인자 및 발암유전자를 조절하기 위한 조성물 및 방법
WO2023230566A2 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulating cytokines
CN114934031B (zh) * 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
KR20250019668A (ko) 2022-05-25 2025-02-10 플래그쉽 파이어니어링 이노베이션스 Vii, 엘엘씨 순환 인자를 조절하기 위한 조성물 및 방법
WO2023230579A2 (en) 2022-05-25 2023-11-30 Metagenomi, Inc. Supplementation of liver enzyme expression
EP4532525A1 (en) 2022-05-25 2025-04-09 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors with cas7-11 variants
WO2023235725A2 (en) 2022-05-31 2023-12-07 Regeneron Pharmaceuticals, Inc. Crispr-based therapeutics for c9orf72 repeat expansion disease
JP2025521154A (ja) 2022-05-31 2025-07-08 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｃ９ｏｒｆ７２反復伸長疾患のためのｃｒｉｓｐｒ干渉療法
WO2023232109A1 (zh) 2022-06-01 2023-12-07 中国科学院遗传与发育生物学研究所 新的crispr基因编辑系统
IL317874A (en) 2022-06-24 2025-02-01 Tune Therapeutics Inc Compounds, systems and methods for reducing low density lipoproteins through targeted gene suppression
UY40326A (es) 2022-06-27 2023-12-29 Pairwise Plants Services Inc Métodos y composiciones para modificar el escape a la sombra en plantas
US20240000031A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN119365481A (zh) 2022-06-29 2025-01-24 成对植物服务股份有限公司 用于控制分生组织大小以进行作物改良的方法和组合物
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion
IL318625A (en) 2022-07-29 2025-03-01 Regeneron Pharma Compositions and methods for transferrin receptor (TFR)-mediated delivery to brain and muscle
US20240043857A1 (en) 2022-08-04 2024-02-08 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
US20240060081A1 (en) 2022-08-11 2024-02-22 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN120112633A (zh) 2022-08-12 2025-06-06 生命编辑治疗股份有限公司 Rna引导的核酸酶及其活性片段和变体以及使用方法
WO2024038168A1 (en) 2022-08-19 2024-02-22 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such
US20240100192A1 (en) 2022-08-25 2024-03-28 Massachusetts Institute Of Technology Programmable rna writing using crispr effectors and trans-splicing templates
CN120322549A (zh) 2022-08-25 2025-07-15 生命编辑治疗股份有限公司 利用锁核酸对向导rna化学修饰以用于rna引导的核酸酶介导的基因编辑
WO2024042165A2 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
US20250386788A1 (en) 2022-08-26 2025-12-25 KWS SAAT SE & Co. KGaA Use of paired genes in hybrid breeding
WO2024042168A1 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024050548A2 (en) * 2022-09-02 2024-03-07 Hunterian Medicine Llc Compact promoters for targeting hypoxia induced genes
JP2025529211A (ja) 2022-09-02 2025-09-04 ヤンセン バイオテツク，インコーポレーテツド 細胞低免疫原性を生物工学で行うための材料及びプロセス
CN120152717A (zh) 2022-09-08 2025-06-13 朱诺治疗学股份有限公司 T细胞疗法和连续或间歇dgk抑制剂给药的组合
US20240090466A1 (en) 2022-09-08 2024-03-21 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
WO2024062138A1 (en) 2022-09-23 2024-03-28 Mnemo Therapeutics Immune cells comprising a modified suv39h1 gene
JP2025532839A (ja) 2022-09-28 2025-10-03 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 細胞に基づく治療を増強させるための抗体耐性改変受容体
WO2024073751A1 (en) 2022-09-29 2024-04-04 Vor Biopharma Inc. Methods and compositions for gene modification and enrichment
WO2024073114A1 (en) 2022-09-30 2024-04-04 Vedabio, Inc. Delivery of therapeutics in vivo via a crispr-based cascade system
US11982677B2 (en) 2022-10-02 2024-05-14 Vedabio, Inc. Dimerization screening assays
WO2024081156A1 (en) 2022-10-14 2024-04-18 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
WO2024083579A1 (en) 2022-10-20 2024-04-25 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
CN120693347A (zh) 2022-11-04 2025-09-23 瑞泽恩制药公司 钙电压门控通道辅助亚基γ1(CACNG1)结合蛋白和CACNG1介导的向骨骼肌的递送
EP4612284A2 (en) 2022-11-04 2025-09-10 Life Edit Therapeutics, Inc. Evolved adenine deaminases and rna-guided nuclease fusion proteins with internal insertion sites and methods of use
EP4612277A1 (en) 2022-11-04 2025-09-10 Iovance Biotherapeutics, Inc. Methods for tumor infiltrating lymphocyte (til) expansion related to cd39/cd103 selection
WO2024098024A1 (en) 2022-11-04 2024-05-10 Iovance Biotherapeutics, Inc. Expansion of tumor infiltrating lymphocytes from liquid tumors and therapeutic uses thereof
CN120239706A (zh) 2022-11-07 2025-07-01 先正达农作物保护股份公司 改变大豆植物开花时间和/或成熟的基因及其用途
EP4615960A1 (en) 2022-11-09 2025-09-17 C3S2 GmbH Methods for manufacturing engineered immune cells
WO2024102434A1 (en) 2022-11-10 2024-05-16 Senda Biosciences, Inc. Rna compositions comprising lipid nanoparticles or lipid reconstructed natural messenger packs
EP4619438A2 (en) 2022-11-14 2025-09-24 Regeneron Pharmaceuticals, Inc. Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes
CN120584182A (zh) 2022-11-21 2025-09-02 艾欧凡斯生物治疗公司 肿瘤浸润淋巴细胞扩增的二维过程及其疗法
WO2024118836A1 (en) 2022-11-30 2024-06-06 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes with shortened rep step
WO2024130102A2 (en) 2022-12-16 2024-06-20 Pairwise Plants Services, Inc. Fusion proteins comprising an intein polypeptide and methods of use thereof
WO2024127369A1 (en) 2022-12-16 2024-06-20 LifeEDIT Therapeutics, Inc. Guide rnas that target foxp3 gene and methods of use
WO2024127370A1 (en) 2022-12-16 2024-06-20 LifeEDIT Therapeutics, Inc. Guide rnas that target trac gene and methods of use
AU2023413056A1 (en) 2022-12-21 2025-06-12 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
WO2024138194A1 (en) 2022-12-22 2024-06-27 Tome Biosciences, Inc. Platforms, compositions, and methods for in vivo programmable gene insertion
CN118275664A (zh) * 2022-12-30 2024-07-02 上海吐露港生物科技有限公司 基于拆分Cas蛋白的非核酸靶标检测方法及应用
WO2024253707A2 (en) 2023-01-07 2024-12-12 Vedabio, Inc. Engineered nucleic acid-guided nucleases
WO2024151493A1 (en) 2023-01-10 2024-07-18 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification
WO2024161021A1 (en) 2023-02-03 2024-08-08 Juno Therapeutics Gmbh Methods for non-viral manufacturing of engineered immune cells
WO2024167317A1 (ko) * 2023-02-10 2024-08-15 주식회사 엔세이지 신규 CRISPR/Cas12a 조성물 및 이의 표적 핵산 검출 용도
TW202440938A (zh) 2023-02-15 2024-10-16 美商喬木生物技術公司 用於抑制微管解聚蛋白2(stmn2)轉錄物中異常剪接的基因編輯方法
US20240279673A1 (en) 2023-02-16 2024-08-22 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
EP4669743A1 (en) 2023-02-22 2025-12-31 KWS SAAT SE & Co. KGaA HIGH-PERFORMANCE PLANTS
CN116355878B (zh) * 2023-02-28 2024-04-26 华中农业大学 新型TnpB编程性核酸酶及其应用
WO2024182746A1 (en) 2023-03-01 2024-09-06 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
AR132019A1 (es) 2023-03-02 2025-05-21 Pairwise Plants Services Inc Métodos y composiciones para modificar la evitación de la sombra en plantas
AR132071A1 (es) 2023-03-09 2025-05-21 Pairwise Plants Services Inc Modificación de genes de la vía de señalización de brasinoesteroide para mejorar rasgos de rendimiento en plantas
EP4680287A1 (en) 2023-03-15 2026-01-21 Renagade Therapeutics Management Inc. Delivery of gene editing systems and methods of use thereof
CN121127605A (zh) 2023-03-29 2025-12-12 阿斯利康(瑞典)有限公司 使用抑制剂来提高CRISPR/Cas插入的效率
PE20252680A1 (es) 2023-03-30 2025-11-24 Pairwise Plants Services Inc Metodos y composiciones para reducir las espinas o espinillas en plantas
WO2024211306A1 (en) 2023-04-03 2024-10-10 Sutro Biopharma, Inc. Rf1 ko e. coli strains
WO2024211287A1 (en) 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
WO2024216116A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
WO2024216118A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
WO2024218295A1 (en) 2023-04-21 2024-10-24 Vib Vzw Allelic combinations in crops for yield increase
CN116751763B (zh) * 2023-05-08 2024-02-13 珠海舒桐医疗科技有限公司 一种Cpf1蛋白、V型基因编辑系统及应用
WO2024234006A1 (en) 2023-05-11 2024-11-14 Tome Biosciences, Inc. Systems, compositions, and methods for targeting liver sinusodial endothelial cells (lsecs)
WO2024235991A1 (en) 2023-05-15 2024-11-21 UCB Biopharma SRL Rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024236547A1 (en) 2023-05-18 2024-11-21 Inceptor Bio, Llc Modified phagocytic cells expressing chimeric antigen receptors comprising a herpes virus entry mediator (hvem) co-stimulatory domain and uses thereof
UY40746A (es) 2023-05-18 2024-12-13 Pairwise Plants Services Inc Métodos y composiciones para mejorar las características de rendimiento de las plantas
WO2024243438A2 (en) 2023-05-23 2024-11-28 Omega Therapeutics, Inc. Compositions and methods for reducing cxcl9, cxcl10, and cxcl11 gene expression
WO2025003358A2 (en) 2023-06-29 2025-01-02 UCB Biopharma SRL Novel nucleic acid targeting systems comprising rna-guided nucleases
WO2025006963A1 (en) 2023-06-30 2025-01-02 Regeneron Pharmaceuticals, Inc. Methods and compositions for increasing homology-directed repair
CN116671491B (zh) * 2023-07-04 2024-01-23 四川省畜牧科学研究院 一种南江黄羊的种羊选育方法
WO2025010912A1 (zh) 2023-07-07 2025-01-16 山东舜丰生物科技有限公司 一种工程化的Cas蛋白及其应用
CN116790559B (zh) * 2023-07-07 2023-11-28 山东舜丰生物科技有限公司 一种融合HNH结构域的V型Cas酶及其应用
US12291729B2 (en) 2023-07-07 2025-05-06 Shandong Shunfeng Biotechnology Co.Ltd. Engineered Cas protein and use thereof
AR133164A1 (es) 2023-07-18 2025-09-03 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectura radicular en plantas
WO2025022367A2 (en) 2023-07-27 2025-01-30 Life Edit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
AR133310A1 (es) 2023-07-27 2025-09-17 Pairwise Plants Services Inc Métodos y composiciones para modificar rasgos de rendimiento de las plantas
US20250041455A1 (en) 2023-07-28 2025-02-06 Regeneron Pharmaceuticals, Inc. Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease
AU2024315073A1 (en) 2023-07-28 2026-01-22 Regeneron Pharmaceuticals, Inc. Use of bgh-sv40l tandem polya to enhance transgene expression during unidirectional gene insertion
AR133384A1 (es) 2023-07-28 2025-09-24 Regeneron Pharma Anti-tfr:esfingomielinasa ácida para el tratamiento de la deficiencia de esfingomielinasa ácida
WO2025027166A1 (en) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Increased resistance by expression of msbp1 protein
WO2025027165A1 (en) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Increased resistance by expression of an ics protein
WO2025027134A1 (en) 2023-08-01 2025-02-06 KWS SAAT SE & Co. KGaA Method of reducing off-flavours in alternative proteins
AU2024319046A1 (en) 2023-08-01 2026-01-29 KWS SAAT SE & Co. KGaA Method of improving gelation properties of alternative proteins
WO2025049524A1 (en) 2023-08-28 2025-03-06 Regeneron Pharmaceuticals, Inc. Cxcr4 antibody-resistant modified receptors
WO2025046513A1 (en) 2023-08-29 2025-03-06 Inceptor Bio, Llc Methods of manufacturing myeloid-derived cells from hematopoietic stem cells and compositions and uses thereof
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025050069A1 (en) 2023-09-01 2025-03-06 Tome Biosciences, Inc. Programmable gene insertion using engineered integration enzymes
WO2025054540A1 (en) 2023-09-08 2025-03-13 Iovance Biotherapeutics, Inc. Methods of gene-editing using programmable nucleases
KR102789465B1 (ko) * 2023-09-20 2025-04-04 주식회사 엔세이지 신규 CRISPR/Cas12a 시스템 및 그 유전자 편집 용도
WO2025072453A1 (en) 2023-09-26 2025-04-03 Pairwise Plants Services, Inc. Engineered deaminases, compositions comprising the same, and methods of use thereof
EP4554371A1 (en) 2023-10-03 2025-05-21 Inari Agriculture Technology, Inc. Viral delivery of grna to the scion
WO2025080537A1 (en) 2023-10-09 2025-04-17 Pairwise Plants Services, Inc. Circular permutants of cas12a
WO2025080600A1 (en) 2023-10-11 2025-04-17 Pairwise Plants Services, Inc. Methods and compositions for improving crop yield traits
WO2025101484A1 (en) 2023-11-06 2025-05-15 Iovance Biotherapeutics, Inc. Treatment of endometrial cancers with tumor infiltrating lymphocyte therapies
EP4560016A1 (en) * 2023-11-22 2025-05-28 Plantik Biosciences Method of plant genome editing
CN117286039B (zh) * 2023-11-27 2024-03-12 中国科学院天津工业生物技术研究所 一种菌丝形态优化的里氏木霉菌株及其应用
WO2025137408A1 (en) 2023-12-21 2025-06-26 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2025132866A1 (en) 2023-12-21 2025-06-26 KWS SAAT SE & Co. KGaA Maize plants with improved disease resistance
WO2025153582A1 (en) 2024-01-17 2025-07-24 KWS SAAT SE & Co. KGaA Maize plants with improved disease resistance
WO2025162985A1 (en) 2024-01-30 2025-08-07 Basf Plant Science Company Gmbh Increased plant disease resistance by expression of a glycine-rich protein
WO2025168705A1 (en) 2024-02-08 2025-08-14 Vib Vzw Means and methods for the production of saponins with endosomal escape-enhancing properties
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining
WO2025174908A1 (en) 2024-02-12 2025-08-21 Life Edit Therapeutics, Inc. Novel rna-guided nucleases and proteins for polymerase editing
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents
WO2025172110A1 (en) 2024-02-16 2025-08-21 Nunhems Netherlands B.V. Modified plant architecture in cucumber
US20250270578A1 (en) 2024-02-22 2025-08-28 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
US12480141B2 (en) 2024-04-04 2025-11-25 Alia Therapeutics Srl Type V Cas proteins and applications thereof
WO2025224107A1 (en) 2024-04-22 2025-10-30 Basecamp Research Ltd Method and compositions for detecting off-target editing
WO2025224182A2 (en) 2024-04-23 2025-10-30 Basecamp Research Ltd Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2025235388A1 (en) 2024-05-06 2025-11-13 Regeneron Pharmaceuticals, Inc. Transgene genomic identification by nuclease-mediated long read sequencing
WO2025237806A1 (en) 2024-05-13 2025-11-20 Nunhems Netherlands B.V. Lettuce plant having delayed bolting and shade tolerance
WO2025240947A1 (en) 2024-05-17 2025-11-20 University Of Massachusetts Therapeutic treatment for fragile x-associated disorder
WO2025265017A1 (en) 2024-06-20 2025-12-26 Regeneron Pharmaceuticals, Inc. Ass1 gene insertion for the treatment of citrullinemia type i
WO2026003754A1 (en) 2024-06-25 2026-01-02 Life Edit Therapeutics, Inc. Novel reverse transcriptases and uses thereof
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A
US20260009010A1 (en) 2024-07-08 2026-01-08 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of cas12a
WO2026015829A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Small reverse transcriptases and gene editing systems comprising such
WO2026015832A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Reverse transcriptases and gene editing systems comprising such
US20260022365A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered cytidine deaminases
WO2026025092A1 (en) 2024-07-26 2026-01-29 Juno Therapeutics, Inc. Synthetic promoters for t cell expression
CN119979720B (zh) * 2025-02-26 2025-09-23 中国农业科学院北京畜牧兽医研究所 猪精液品质相关的分子标记鉴定及其应用
CN121343008A (zh) * 2025-03-21 2026-01-16 华东理工大学 一种dhodh多肽降解剂及其在制备预防和/或治疗dhodh介导的疾病的药物中的应用

Family Cites Families (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1506509A (en) 1975-12-19 1978-04-05 Nissan Motor Apparatus for mounting instruments on the instrument panel of a motor vehicle
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
JP2874751B2 (ja) 1986-04-09 1999-03-24 ジェンザイム・コーポレーション 希望する蛋白質をミルク中へ分泌する遺伝子移植動物
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
WO1991017424A1 (en) 1990-05-03 1991-11-14 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5266317A (en) 1990-10-04 1993-11-30 University Of Georgia Research Foundation, Inc. Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
GB9104617D0 (en) 1991-03-05 1991-04-17 Nickerson Int Seed Pest control
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
DE4124514A1 (de) 1991-07-24 1993-01-28 Bosch Gmbh Robert Beschleunigungssensor, insbesondere zum selbsttaetigen ausloesen von insassenschutzvorrichtungen in kraftfahrzeugen
DK39692D0 (da) 1992-03-25 1992-03-25 Danisco Biologisk materiale
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5593972A (en) 1993-01-26 1997-01-14 The Wistar Institute Genetic immunization
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
US6007845A (en) 1994-07-22 1999-12-28 Massachusetts Institute Of Technology Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers
US5994627A (en) 1995-03-31 1999-11-30 Common Wealth Scientific And Industrial Research Organisation Genetic sequences conferring nematode resistance in plants and uses therefor
CA2223103A1 (en) 1995-06-06 1996-12-12 Isis Pharmaceuticals Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
AR006928A1 (es) 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
US5855913A (en) 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
US5985309A (en) 1996-05-24 1999-11-16 Massachusetts Institute Of Technology Preparation of particles for inhalation
US5846946A (en) 1996-06-14 1998-12-08 Pasteur Merieux Serums Et Vaccins Compositions and methods for administering Borrelia DNA
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US7022896B1 (en) 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
GB9720465D0 (en) 1997-09-25 1997-11-26 Oxford Biomedica Ltd Dual-virus vectors
IL135776A0 (en) 1997-10-24 2001-05-20 Life Technologies Inc Recombinational cloning using nucleic acids having recombination sites
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7868149B2 (en) 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
GB0024550D0 (ru) 2000-10-06 2000-11-22 Oxford Biomedica Ltd
WO2003045304A2 (en) 2001-09-26 2003-06-05 Mayo Foundation For Medical Education And Research Mutable vaccines
GB0125216D0 (en) 2001-10-19 2001-12-12 Univ Strathclyde Dendrimers for use in targeted delivery
US20090100536A1 (en) 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
JP2005512598A (ja) 2001-12-21 2005-05-12 オックスフォード バイオメディカ （ユーケー） リミテッド Ｅｉａｖなどのレンチウイルス発現ベクターを使用するトランスジェニック生物の作製方法
AU2003245160B2 (en) 2002-06-28 2009-09-24 Arbutus Biopharma Corporation Method and apparatus for producing liposomes
GB0220467D0 (en) 2002-09-03 2002-10-09 Oxford Biomedica Ltd Composition
AU2003284388A1 (en) 2002-11-01 2004-06-07 New England Biolabs, Inc. Organellar targeting of rna and its use in the interruption of environmental gene flow
KR101168440B1 (ko) 2003-07-16 2012-07-27 프로티바 바이오쎄라퓨틱스, 인코포레이티드 지질 캡슐화된 간섭 ｒｎａ
AU2004272646B2 (en) 2003-09-15 2011-11-24 Arbutus Biopharma Corporation Polyethyleneglycol-modified lipid compounds and uses thereof
GB0325379D0 (en) 2003-10-30 2003-12-03 Oxford Biomedica Ltd Vectors
US7919277B2 (en) * 2004-04-28 2011-04-05 Danisco A/S Detection and typing of bacterial strains
US7745651B2 (en) 2004-06-07 2010-06-29 Protiva Biotherapeutics, Inc. Cationic lipids and methods of use
US7799565B2 (en) 2004-06-07 2010-09-21 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering RNA
US20090214588A1 (en) 2004-07-16 2009-08-27 Nabel Gary J Vaccines against aids comprising cmv/r-nucleic acid constructs
GB0422877D0 (en) 2004-10-14 2004-11-17 Univ Glasgow Bioactive polymers
US7838658B2 (en) 2005-10-20 2010-11-23 Ian Maclachlan siRNA silencing of filovirus gene expression
US8044019B2 (en) 2005-10-28 2011-10-25 Mitsubishi Tanabe Pharma Corporation Cell penetrating peptide
CN101346393B (zh) 2005-11-02 2015-07-22 普洛体维生物治疗公司 修饰的siRNA分子及其应用
GB0526211D0 (en) 2005-12-22 2006-02-01 Oxford Biomedica Ltd Viral vectors
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
JP2008078613A (ja) 2006-08-24 2008-04-03 Rohm Co Ltd 窒化物半導体の製造方法及び窒化物半導体素子
US20100099858A1 (en) 2006-09-28 2010-04-22 Mirkin Chad A Maximizing Oligonucleotide Loading on Gold Nanoparticle
JP5690068B2 (ja) 2007-01-11 2015-03-25 エラスムス ユニバーシティ メディカル センター 環状染色体コンホメーション捕捉（４ｃ）
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
EP2178585B1 (en) 2007-08-14 2021-04-07 Fred Hutchinson Cancer Research Center Needle array assembly and method for delivering therapeutic agents
AU2008333811B2 (en) 2007-12-04 2014-05-01 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
WO2009088786A1 (en) 2007-12-31 2009-07-16 Nanocor Therapeutics, Inc. Rna interference for the treatment of heart failure
NZ588583A (en) 2008-04-15 2012-08-31 Protiva Biotherapeutics Inc Novel lipid formulations for nucleic acid delivery
FR2930264B1 (fr) * 2008-04-18 2013-02-22 Gervais Danone Sa Nouvelle souche de lactobacillus paracasei subsp. paracasei dotee de proprietes antimicrobiennes et immunomodulatrices.
EP2283029A1 (en) 2008-06-04 2011-02-16 Medical Research Council Peptides
WO2010001325A2 (en) 2008-06-30 2010-01-07 Silenseed Ltd Methods, compositions and systems for local delivery of drugs
EP2309980A1 (en) 2008-07-08 2011-04-20 S.I.F.I. Societa' Industria Farmaceutica Italiana Ophthalmic compositions for treating pathologies of the posterior segment of the eye
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
AU2009311667B2 (en) 2008-11-07 2016-04-14 Massachusetts Institute Of Technology Aminoalcohol lipidoids and uses thereof
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
US20120164118A1 (en) 2009-05-04 2012-06-28 Fred Hutchinson Cancer Research Center Cocal vesiculovirus envelope pseudotyped retroviral vectors
EP2449114B9 (en) 2009-07-01 2017-04-19 Protiva Biotherapeutics Inc. Novel lipid formulations for delivery of therapeutic agents to solid tumors
WO2011000108A1 (en) 2009-07-01 2011-01-06 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein b
WO2011028929A2 (en) 2009-09-03 2011-03-10 The Regents Of The University Of California Nitrate-responsive promoter
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
BR112012015755B1 (pt) 2009-12-23 2021-06-22 Novartis Ag Lipídeo furtivo, e composição
KR101956623B1 (ko) 2010-02-24 2019-03-12 애로우헤드 파마슈티컬스 인코포레이티드 ｓｉＲＮＡ의 표적 전달용 조성물
CN102939377B (zh) 2010-04-26 2016-06-08 桑格摩生物科学股份有限公司 使用锌指核酸酶对Rosa位点进行基因组编辑
US8372951B2 (en) 2010-05-14 2013-02-12 National Tsing Hua University Cell penetrating peptides for intracellular delivery
JP5965392B2 (ja) 2010-05-28 2016-08-03 オックスフォード バイオメディカ （ユーケー） リミテッド 脳へのレンチウイルスベクターの送達
EP2609135A4 (en) 2010-08-26 2015-05-20 Massachusetts Inst Technology POLY (BETA AMINO ALCOHOLS), THEIR PREPARATION AND USES THEREOF
EA201390586A1 (ru) * 2010-10-20 2014-11-28 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Последовательности crispr-cas lactococcus
US9405700B2 (en) 2010-11-04 2016-08-02 Sonics, Inc. Methods and apparatus for virtualization in an integrated circuit
US9238716B2 (en) 2011-03-28 2016-01-19 Massachusetts Institute Of Technology Conjugated lipomers and uses thereof
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US20120295960A1 (en) 2011-05-20 2012-11-22 Oxford Biomedica (Uk) Ltd. Treatment regimen for parkinson's disease
CN103826455B (zh) 2011-09-26 2015-10-21 住友化学株式会社 植物病害防治组合物及其用途
HRP20220717T1 (hr) 2011-12-16 2022-07-22 Modernatx, Inc. Modificirani pripravci mrna
US20130185823A1 (en) 2012-01-16 2013-07-18 Academia Sinica Mesoporous silica nanoparticle-mediated delivery of dna into arabidopsis root
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
KR20150001710A (ko) 2012-04-18 2015-01-06 애로우헤드 리서치 코오포레이션 Ｉｎ Ｖｉｖｏ 핵산 전달용 폴리(아크릴레이트) 고분자
US20160046961A1 (en) 2012-05-25 2016-02-18 Emmanuelle Charpentier Methods and Compositions for RNA-Directed Target DNA Modification and For RNA-Directed Modulation of Transcription
US8614194B1 (en) 2012-07-25 2013-12-24 Kaohsiung Medical University Anionic cell penetrating peptide and its use for intracellular delivery
DK3494997T3 (da) 2012-07-25 2019-12-02 Broad Inst Inc Inducerbare dna-bindende proteiner og værktøjer til genomperturbation samt anvendelser deraf
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
DK2898075T3 (en) 2012-12-12 2016-06-27 Broad Inst Inc CONSTRUCTION AND OPTIMIZATION OF IMPROVED SYSTEMS, PROCEDURES AND ENZYME COMPOSITIONS FOR SEQUENCE MANIPULATION
US20140189896A1 (en) 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
WO2014093694A1 (en) * 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
KR20150105956A (ko) 2012-12-12 2015-09-18 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작 및 치료적 적용을 위한 시스템, 방법 및 조성물의 전달, 유전자 조작 및 최적화
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
ES2536353T3 (es) 2012-12-12 2015-05-22 The Broad Institute, Inc. Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias
SG10201912991WA (en) 2012-12-17 2020-03-30 Harvard College Rna-guided human genome engineering
WO2014118272A1 (en) 2013-01-30 2014-08-07 Santaris Pharma A/S Antimir-122 oligonucleotide carbohydrate conjugates
GB2527450A (en) 2013-03-14 2015-12-23 Caribou Biosciences Inc Compositions and methods of nucleic acid-targeting nucleic acids
CA2913869C (en) 2013-05-29 2023-01-24 Cellectis New compact scaffold of cas9 in the type ii crispr system
US9267135B2 (en) 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
RU2756865C2 (ru) * 2013-06-04 2021-10-06 Президент Энд Фэллоуз Оф Харвард Коллидж Направляемая рнк регуляция транскрипции
EP3004370B1 (en) * 2013-06-05 2024-08-21 Duke University Rna-guided gene editing and gene regulation
EP3011034B1 (en) * 2013-06-17 2019-08-07 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
CN106062197A (zh) 2013-06-17 2016-10-26 布罗德研究所有限公司 用于序列操纵的串联指导系统、方法和组合物的递送、工程化和优化
US10563225B2 (en) 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
ES3013744T3 (en) 2013-09-18 2025-04-15 Kymab Ltd Methods, cells and organisms
DK3066201T3 (en) 2013-11-07 2018-06-06 Editas Medicine Inc CRISPR-RELATED PROCEDURES AND COMPOSITIONS WITH LEADING GRADES
WO2015071474A2 (en) 2013-11-18 2015-05-21 Crispr Therapeutics Ag Crispr-cas system materials and methods
EP3080271B1 (en) 2013-12-12 2020-02-12 The Broad Institute, Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
BR112016013213A2 (pt) * 2013-12-12 2017-12-05 Massachusetts Inst Technology administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para visar distúrbios e doenças usando componentes de administração de partículas
MX2016007326A (es) 2013-12-12 2017-02-13 Broad Inst Inc Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos.
EP4219699A1 (en) 2013-12-12 2023-08-02 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
CA2933134A1 (en) 2013-12-13 2015-06-18 Cellectis Cas9 nuclease platform for microalgae genome engineering
AU2014378946B2 (en) 2014-01-21 2018-04-19 Suzhou Qi Biodesign Biotechnology Company Limited Modified plants
JP5937635B2 (ja) 2014-03-28 2016-06-22 ファナック株式会社 電磁接触器の溶着検出機能を有するモータ駆動装置
CN104017821B (zh) * 2014-05-16 2016-07-06 安徽省农业科学院水稻研究所 定向编辑颖壳颜色决定基因OsCHI创制褐壳水稻材料的方法
WO2016049258A2 (en) 2014-09-25 2016-03-31 The Broad Institute Inc. Functional screening with optimized functional crispr-cas systems
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
SG10201906070XA (en) * 2014-12-31 2019-08-27 Synthetic Genomics Inc Compositions and methods for high efficiency in vivo genome editing
CA3060508C (en) * 2015-01-28 2023-08-29 Pioneer Hi-Bred International, Inc. Crispr hybrid dna/rna polynucleotides and methods of use
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US20160362667A1 (en) * 2015-06-10 2016-12-15 Caribou Biosciences, Inc. CRISPR-Cas Compositions and Methods
CA3012607A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzymes and systems
EP3666895A1 (en) * 2015-06-18 2020-06-17 The Broad Institute, Inc. Novel crispr enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US20190048340A1 (en) 2015-09-24 2019-02-14 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
CN105602993A (zh) * 2016-01-19 2016-05-25 上海赛墨生物技术有限公司 线粒体靶向的基因编辑系统及方法
CA3026110A1 (en) 2016-04-19 2017-11-02 The Broad Institute, Inc. Novel crispr enzymes and systems
US20230037794A1 (en) 2019-12-23 2023-02-09 The Broad Institute, Inc. Programmable dna nuclease-associated ligase and methods of use thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2720769C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/CAS и препарат для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
RU2720768C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
RU2720767C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ обнаружения провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях и специфические олигонуклеотиды для использования в способе
RU2743861C1 (ru) * 2020-04-15 2021-03-01 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для выявления гена антибиотикоустойчивости blaVIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
RU2745637C1 (ru) * 2020-04-15 2021-03-29 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/Cas и препарат для выявления гена антибиотикоустойчивости bla VIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
WO2021211010A1 (ru) * 2020-04-15 2021-10-21 Федеральное Бюджетное Учреждение Науки "Центральный Научно-Исследовательский Институт Эпидемиологии" Федеральной Службы По Надзору В Сфере Защиты Прав Потребителей И Благополучия Человека Crispr/cas для выявления гена антибиотикоустойчивости bla vim-2

Also Published As

Publication number Publication date
JP7731398B2 (ja) 2025-08-29
US20160208243A1 (en) 2016-07-21
ES2791398T3 (es) 2020-11-04
EP4403638A9 (en) 2025-10-29
WO2016205711A1 (en) 2016-12-22
MX2020011939A (es) 2021-01-29
IL305044B1 (en) 2025-01-01
DK3604532T3 (da) 2024-07-22
KR102613296B1 (ko) 2023-12-13
DK3502253T3 (da) 2020-08-17
JP7280312B2 (ja) 2023-05-23
AU2021200010A1 (en) 2021-02-11
US20180155716A1 (en) 2018-06-07
EP3009511B1 (en) 2017-05-31
AU2024202007A1 (en) 2024-04-18
EP3470519B1 (en) 2020-02-12
CN108513582A (zh) 2018-09-07
CA2989834A1 (en) 2016-12-22
KR20180034402A (ko) 2018-04-04
CN109207477B (zh) 2021-07-16
JP2025179085A (ja) 2025-12-09
CN115572727A (zh) 2023-01-06
DK3470519T3 (da) 2020-05-04
FI3310917T4 (fi) 2025-12-10
CN115572718A (zh) 2023-01-06
EP3502253A1 (en) 2019-06-26
AU2021200010B2 (en) 2024-01-18
EP3604532B1 (en) 2024-05-01
IL290678B1 (en) 2023-09-01
HK1253001A1 (en) 2019-06-06
RU2018101666A3 (ru) 2020-04-28
AU2016278990B2 (en) 2020-10-22
EP4403638A3 (en) 2025-03-26
SG10201912330QA (en) 2020-02-27
DK3009511T3 (da) 2017-09-11
JP2023100906A (ja) 2023-07-19
IL305044A (en) 2023-10-01
EP3310917B2 (en) 2025-09-03
FI3604532T3 (fi) 2024-08-05
RU2020136363A (ru) 2020-12-24
EP3009511A3 (en) 2016-06-29
JP2021151247A (ja) 2021-09-30
JP2018518183A (ja) 2018-07-12
AU2016278990A1 (en) 2018-01-18
EP4403638A2 (en) 2024-07-24
EP3502253B1 (en) 2020-05-27
ES2994064T3 (en) 2025-01-16
EP3470519A1 (en) 2019-04-17
DK3310917T3 (da) 2020-05-04
HK1220726A1 (en) 2017-05-12
CN109207477A (zh) 2019-01-15
TWI758251B (zh) 2022-03-21
ES2791195T3 (es) 2020-11-03
MX377162B (es) 2025-03-07
IL290678A (en) 2022-04-01
DK3310917T4 (da) 2025-12-15
EP3310917B1 (en) 2020-02-12
EP3009511B2 (en) 2021-01-27
US9790490B2 (en) 2017-10-17
TW202426636A (zh) 2024-07-01
IL290678B2 (en) 2024-01-01
CN115572737A (zh) 2023-01-06
IL256369B (en) 2022-03-01
EP3604532A1 (en) 2020-02-05
ES2646140T3 (es) 2017-12-12
EP3502253B2 (en) 2025-11-19
IL305044B2 (en) 2025-05-01
RU2737537C2 (ru) 2020-12-01
EP3009511A2 (en) 2016-04-20
JP6898865B2 (ja) 2021-07-07
CN114032250A (zh) 2022-02-11
TWI837592B (zh) 2024-04-01
KR20230170151A (ko) 2023-12-18
US10669540B2 (en) 2020-06-02
MX2017016688A (es) 2018-12-11
TW202223088A (zh) 2022-06-16
TW201716572A (zh) 2017-05-16
CN108513582B (zh) 2022-08-05
EP3310917A1 (en) 2018-04-25
IL256369A (en) 2018-02-28

Similar Documents

Publication Publication Date Title
RU2018101666A (ru) Новые ферменты и системы crispr
ES2985812T3 (es) Edición genómica de precisión de alto rendimiento
ES2653212T3 (es) Modificación y regulación del genoma en base a CRISPR
US10767174B2 (en) Method for RGEN RNP delivery using 5′-phosphate removed RNA
JP2021164463A (ja) エピゲノム編集のための組成物および方法
FI3445388T3 (fi) Materiaaleja ja menetelmiä hemoglobinopatioiden hoitamiseksi
EP3080256B1 (en) Cas9 nuclease platform for microalgae genome engineering
JP6835726B2 (ja) Ｃｒｉｓｐｒハイブリッドｄｎａ／ｒｎａポリヌクレオチドおよび使用方法
ES2884838T3 (es) ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS
JP2025121906A5 (ru)
AU2020346880B2 (en) Modified bacterial retroelement with enhanced DNA production
RU2018101710A (ru) Мутации фермента crispr, уменьшающие нецелевые эффекты
CN109312308A (zh) 通过使用核酸酶来进行人神经干细胞的基因组编辑
JP2018534114A5 (ru)
KR20200036025A (ko) 메신저 리보핵산(mRNA)을 암호화하는 안정화된 핵산
KR20180037297A (ko) 상동 재조합에 의한 crispr/cas-기반 게놈 편집을 위한 화합물 및 방법
KR20190089175A (ko) 표적 핵산 변형을 위한 조성물 및 방법
AR107621A2 (es) Plantas de algodón con tolerancia a herbicidas y métodos para identificarlas
CN107354173A (zh) 基于crispr技术和水动力尾静脉注射建立肝脏特异性敲除小鼠模型的方法
CN109415729A (zh) 具有降低毒性的基因编辑试剂
JP2025078704A (ja) トリプタミン発現を調整する真菌の遺伝子操作
CN113366106A (zh) 用于递送转基因的组合物和方法
WO2018117746A1 (ko) 동물 배아의 염기 교정용 조성물 및 염기 교정 방법
WO2025038982A2 (en) Regulators of t cell exhaustion
KR102515727B1 (ko) 중첩된 가이드핵산을 이용한 표적 핵산에 특정 핵산 서열을 삽입하기 위한 조성물 및 방법

Legal Events

Date Code Title Description
HC9A Changing information about inventors