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AR006928A1 - Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas. - Google Patents

Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.

Info

Publication number
AR006928A1
AR006928A1 ARP970101802A ARP970101802A AR006928A1 AR 006928 A1 AR006928 A1 AR 006928A1 AR P970101802 A ARP970101802 A AR P970101802A AR P970101802 A ARP970101802 A AR P970101802A AR 006928 A1 AR006928 A1 AR 006928A1
Authority
AR
Argentina
Prior art keywords
plants
cells
fluorescent protein
expression
protein
Prior art date
Application number
ARP970101802A
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English (en)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR006928A1 publication Critical patent/AR006928A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Las moléculas de ADN se proveen para transformación de células en las cuales la expresión de un gen que codifica la proteina fluorescente verdeestá situada bajo el control de un promotor inducible. Además, se proveen moléculas de ADN enl as cuales una secuencia nucleotídica que codifica laproteina fluorescente verde, está ligada operativamente a una secuencia de senal que dirigela porteina expresada a uncompartimiento subcelular dondela proteína no es tóxica para la célula.Las tensiones oxidativas para las células vegetales transformadas con GFP pueden mejorar también mediantetransformación de las células con un vector de expresión que comprende genes que codifican GFP y una enzima depuradora de oxígenotalcomo sup eróxidodismutasa. La toxicidad de GFP en las plantas transformadaspuede eliminarse extirpando el gen marcador rastreable a continuación de las células o sectorestransformados. El sistema FLP/FRT se usa conjuntamente con GFP comomarcador visib le para la transformación y excisión de FRT. Se provee también unamolécula nucleotídica optimizada para la expresión de una proteína fluorescente verde en plantas. Un método para la producción de palntastransgénicas enel que un vector p ortador de un gen que codifica la proteína fluorescente verde se introduce en las células, las células son rastreadas paradeterminar la proteína y las células transformadas son seleccionadas y regeneradas. La toxicidadcelular de la prote ína fluorescente estácircunscripta por la expresión reguladora del gen que codifica la proteína o a la dirección de la proteína a un compartimiento subcelular donde no estóxica para la célula. También comprende un vector deexpresión que compren de dicha molécula de adn, un método para usar dicho vector de expresión una
ARP970101802A 1996-05-01 1997-04-30 Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas. AR006928A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1634596P 1996-05-01 1996-05-01

Publications (1)

Publication Number Publication Date
AR006928A1 true AR006928A1 (es) 1999-09-29

Family

ID=21776667

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP970101802A AR006928A1 (es) 1996-05-01 1997-04-30 Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.

Country Status (8)

Country Link
US (1) US6486382B1 (es)
EP (1) EP0904371B1 (es)
AR (1) AR006928A1 (es)
AT (1) ATE277177T1 (es)
AU (1) AU730927B2 (es)
CA (1) CA2252412C (es)
DE (1) DE69730830T2 (es)
WO (1) WO1997041228A2 (es)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1772519A1 (en) * 1997-01-17 2007-04-11 The Scripps Research Institute RNA binding protein and binding site useful for expression of recombinant molecules
JP2002513283A (ja) 1997-01-17 2002-05-08 ザ スクリップス リサーチ インスティテュート 組換え分子の発現に有用なｒｎａ結合タンパク質及び結合部位
US7022896B1 (en) 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
RU2226215C2 (ru) 1997-04-04 2004-03-27 Борд Оф Риджентс Оф Юниверсити Оф Нибраска Молекула днк, кодирующая дикамба-разрушающую оксигеназу, и ее применение
US7105724B2 (en) 1997-04-04 2006-09-12 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
AU7022998A (en) * 1997-04-28 1998-11-24 Mcgill University Reporter fusion proteins, expression vectors and transfected cell lines thereof for the analysis of nuclear transport
US6639050B1 (en) 1997-07-21 2003-10-28 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US6570062B1 (en) * 1997-07-21 2003-05-27 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US7378506B2 (en) * 1997-07-21 2008-05-27 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
IL122270A0 (en) * 1997-11-20 1998-04-05 Yeda Res & Dev DNA molecules conferring to plants resistance to a herbicide and plants transformed thereby
US6541684B1 (en) 1998-02-13 2003-04-01 Trustees Of Columbia University In The City Of New York Nucleotide sequences encoding maize RAD51
EP1925320A3 (en) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
US6307123B1 (en) * 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
US6388169B1 (en) * 1998-06-08 2002-05-14 Pioneer Hi-Bred International, Inc. Maize orthologues of bacterial RecA proteins
US6720478B1 (en) 1999-05-05 2004-04-13 Pioneer Hi-Bred International, Inc. RAD51-like polynucleotide and uses thereof
AU771177B2 (en) * 1999-05-05 2004-03-18 Pioneer Hi-Bred International, Inc. A novel maize RAD51-like gene and uses thereof
WO2000068390A2 (en) * 1999-05-11 2000-11-16 E.I. Du Pont De Nemours And Company Plant recombination proteins
US6852910B1 (en) 1999-05-11 2005-02-08 Omolayo O. Famodu Plant recombination proteins
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
CA2387876A1 (en) * 1999-10-15 2001-04-26 Calgene Llc Methods and vectors for site-specific recombination in plant cell plastids
US6849778B1 (en) 1999-10-15 2005-02-01 Calgene Llc Methods and vectors for site-specific recombination in plant cell plastids
US7126041B1 (en) 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
US7109315B2 (en) 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
KR100414641B1 (ko) * 2000-04-07 2004-01-13 동부한농화학 주식회사 형질전환식물체의 생체분석방법 및 그를 이용한 시스템
US6617494B2 (en) 2000-04-12 2003-09-09 Paradigm Genetics, Inc. Methods for identifying transgenic plants using morphological markers
DE10038573A1 (de) * 2000-08-03 2002-02-21 Mpb Cologne Gmbh Molecular Pla Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen
US6696621B2 (en) 2001-02-23 2004-02-24 Paradigm Genetics, Inc. Selectable marker in plants
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
US20020188965A1 (en) 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
SG182847A1 (en) 2001-05-30 2012-08-30 Agrisoma Inc Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
FR2825579B1 (fr) * 2001-06-11 2004-07-23 Rhobio Methode d'obtention de plante monocotyledone contenant un gene d'interet sans sequence auxiliaire etrangere
EP1440146A4 (en) * 2001-06-26 2005-06-22 Genome Biosciences Llc PROCESS AND VECTORS FOR THE TARGETING OF GENES
US8022272B2 (en) 2001-07-13 2011-09-20 Sungene Gmbh & Co. Kgaa Expression cassettes for transgenic expression of nucleic acids
EP1279737A1 (en) * 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformation method for obtaining marker-free plants
US7456335B2 (en) 2001-09-03 2008-11-25 Basf Plant Science Gmbh Nucleic acid sequences and their use in methods for achieving pathogen resistance in plants
GB0122828D0 (en) * 2001-09-21 2001-11-14 Univ Cambridge Tech Gene expression construct
KR100459107B1 (ko) * 2002-03-21 2004-12-03 한국해양연구원 녹색형광단백질 유전자를 이용한 삭시톡신 검출용바이오센서
CA2883729C (en) 2002-05-22 2017-03-07 Virginia M. Ursin Fatty acid desaturases from fungi
US7786344B2 (en) 2002-07-26 2010-08-31 Basf Plant Science Gmbh Selection method
CA2505974A1 (en) * 2002-11-12 2004-05-27 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same
DK1576157T3 (da) * 2002-12-26 2010-11-01 Zakrytoe Aktsionernoe Obschest Fluorescerende proteiner fra Copepode-arter og fremgangsmåder til anvendelse af samme
US20040210961A1 (en) 2003-03-07 2004-10-21 Palys Joseph Michael Markerless transformation
US20060252120A1 (en) * 2003-05-09 2006-11-09 Kieliszewski Marcia J Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
EP2330206A1 (en) 2003-08-11 2011-06-08 Kweek-en Researchbedrijf Agrico B.V. A gene from Solanum bulbocastanum conferring resistance to Phytophthora infestans
EP2361984A1 (en) 2003-10-09 2011-08-31 E. I. du Pont de Nemours and Company Gene silencing by using modified micro-RNA molecules
US20060026719A1 (en) * 2004-01-14 2006-02-02 Kieliszewski Marcia J Methods of producing peptides/proteins in plants and peptides/proteins produced thereby
CA2573918A1 (en) * 2004-04-19 2005-11-24 Ohio University Cross-linkable glycoproteins and methods of making the same
CA2834275A1 (en) 2004-08-02 2006-02-09 Basf Plant Science Gmbh Method for isolation of transcription termination sequences
CA2573775C (en) * 2004-08-11 2017-08-15 Orf Liftaekni Hf. Traceability of transgenic plant seeds in upstream and downstream processing
AU2006222012B2 (en) 2005-03-08 2011-03-31 Basf Plant Science Gmbh Expression enhancing intron sequences
BRPI0608829A2 (pt) 2005-04-19 2011-03-15 Basf Plant Science Gmbh método para a expressão transgênica com especificidade intensificada em uma planta, uso de um construto de ácido nucleico quimérico, seqüência de ribonucleotìdeo quimérica, construto de expressão, vetor de expressão, organismo não-humano ou célula transformada, semente transformada, e, preparação farmacêutica
CA2606220A1 (en) 2005-04-19 2006-12-21 Basf Plant Science Gmbh Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants
AR053279A1 (es) * 2005-05-19 2007-04-25 Monsanto Technology Llc Regulacion post-transcripcional de la expresion genica
US7994399B2 (en) 2005-06-23 2011-08-09 Basf Plant Science Gmbh Methods for the production of stably transformed, fertile Zea mays plants
WO2007008708A2 (en) * 2005-07-08 2007-01-18 Ohio University Methods of predicting hyp-glycosylation sites for proteins expressed and secreted in plant cells, and related methods and products
WO2007039454A1 (en) 2005-09-20 2007-04-12 Basf Plant Science Gmbh Methods for controlling gene expression using ta-siran
US8859846B2 (en) 2005-09-21 2014-10-14 E. I. Du Pont De Nemours And Company Doubling of chromosomes in haploid embryos
AU2006311089C1 (en) 2005-11-08 2012-11-08 Basf Plant Science Gmbh Use of armadillo repeat (ARM1) polynucleotides for obtaining pathogen resistance in plants
US8178751B2 (en) 2006-01-12 2012-05-15 Basf Plant Science Gmbh Use of stomatin (STM1) polynucleotides for achieving a pathogen resistance in plants
US20080313769A9 (en) 2006-01-12 2008-12-18 Athenix Corporation EPSP synthase domains conferring glyphosate resistance
US7989679B2 (en) 2006-03-02 2011-08-02 Athenix Corp. Methods and compositions for improved enzyme activity in transgenic plants
CA2917552C (en) 2006-05-12 2018-09-25 Monsanto Technology Llc Nucleic acid molecules encoding a phytoene synthase
US7855326B2 (en) 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
US7884262B2 (en) 2006-06-06 2011-02-08 Monsanto Technology Llc Modified DMO enzyme and methods of its use
AU2007257925B2 (en) 2006-06-06 2012-07-05 Monsanto Technology Llc Method for selection of transformed cells
DK2059600T3 (da) 2006-08-30 2014-07-07 Basf Plant Science Gmbh Fremgangsmåde til forøgelse af patogenresistensen i transgene planter
BRPI0716373B1 (pt) * 2006-08-31 2017-10-10 Monsanto Technology Llc "processes for production of a transgenic corn plant".
US8680235B2 (en) 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
US20080115240A1 (en) 2006-10-05 2008-05-15 Milo Aukerman Maize microrna sequences
EP2078087A1 (de) 2006-10-12 2009-07-15 BASF Plant Science GmbH Verfahren zur erhöhung der pathogenresistenz in transgenen pflanzen
EP2081954B1 (de) 2006-10-24 2012-06-13 BASF Plant Science GmbH Verfahren zur erhöhung der resistenz gegen biotrophe pilze in pflanzen
US7939721B2 (en) 2006-10-25 2011-05-10 Monsanto Technology Llc Cropping systems for managing weeds
US20100162433A1 (en) 2006-10-27 2010-06-24 Mclaren James Plants with improved nitrogen utilization and stress tolerance
CA2673361A1 (en) 2007-01-15 2008-07-24 Basf Plant Science Gmbh Use of subtilisin (rnr9) polynucleotides for achieving a pathogen resistance in plants
US20110035840A1 (en) 2007-02-16 2011-02-10 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledoneous plants
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
RU2351039C1 (ru) * 2007-08-23 2009-03-27 Институт автоматики и электрометрии Сибирского отделения Российской академии наук Термофотоэлектрический преобразователь
UA108597C2 (uk) 2008-03-04 2015-05-25 Вашингтон Стейт Юніверсіті Спосіб регуляції ненасиченості жирних кислот олії насіння
US20110076296A1 (en) 2008-04-25 2011-03-31 Innate Pharma S.A. TLR3 Agonist Compositions
CN101768213B (zh) 2008-12-30 2012-05-30 中国科学院遗传与发育生物学研究所 一种与植物分蘖数目相关的蛋白及其编码基因与应用
CN101817879A (zh) 2009-02-26 2010-09-01 中国科学院遗传与发育生物学研究所 金属硫蛋白及其编码基因与应用
ES2988190T3 (es) 2009-04-16 2024-11-19 Benson Hill Seeds Inc Soja con altas tasas de germinación y contenido ultrabajo de rafinosa y estaquiosa
EP2421976B1 (en) 2009-04-22 2015-09-16 BASF Plant Science Company GmbH Whole seed specific promoter
EP2272862B1 (en) 2009-07-10 2012-03-21 Freie Universität Berlin Rock2 and rock3, two new gain-of-function variants of the cytokinin receptors AHK2 and AHK3
WO2011004028A2 (en) 2009-07-10 2011-01-13 Innate Pharma Tlr3 binding agents
EP2275564A1 (en) 2009-07-17 2011-01-19 Freie Universität Berlin Means and method for the production of transgenic plants that are resistant to clubroot
US8937214B2 (en) 2009-10-23 2015-01-20 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
AU2010325564A1 (en) 2009-11-27 2012-07-12 Basf Plant Science Company Gmbh Chimeric endonucleases and uses thereof
WO2011064750A1 (en) 2009-11-27 2011-06-03 Basf Plant Science Company Gmbh Chimeric endonucleases and uses thereof
BR112012012588B1 (pt) 2009-11-27 2019-03-26 Basf Plant Science Company Gmbh Endonuclease, método para recombinação homóloga de polinucleotídeos e método para mutação direcionada de polinucleotídeos
WO2011146754A1 (en) 2010-05-19 2011-11-24 The Samuel Roberts Noble Foundation, Inc. Altered leaf morphology and enhanced agronomic properties in plants
US9045549B2 (en) 2010-11-03 2015-06-02 The Samuel Roberts Noble Foundation, Inc. Transcription factors for modification of lignin content in plants
WO2012095432A2 (en) 2011-01-12 2012-07-19 Innate Pharma Tlr3 binding agents
EP2713736B1 (en) 2011-06-01 2023-08-02 Janus Biotherapeutics, Inc. Novel immune system modulators
EP2713737B1 (en) 2011-06-01 2016-04-20 Janus Biotherapeutics, Inc. Novel immune system modulators
WO2013052550A2 (en) 2011-10-04 2013-04-11 Janus Biotherapeutics, Inc. Novel imidazole quinoline-based immune system modulators
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
MX361773B (es) 2012-05-31 2018-12-17 Innate Pharma Agentes de ligación a tlr3.
US20150240253A1 (en) 2012-08-30 2015-08-27 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
CN104736699B (zh) 2012-09-14 2020-04-14 拜尔作物科学有限合伙公司 Hppd变体及使用方法
DK3622810T5 (da) 2012-09-24 2024-09-02 Seminis Vegetable Seeds Inc Fremgangsmåder og sammensætninger til forlængelse af holdbarheden af planteprodukter
CA2942171C (en) 2014-03-11 2023-05-09 Bayer Cropscience Lp Hppd variants and methods of use
JP2017525711A (ja) 2014-08-22 2017-09-07 ジャナス バイオセラピューティクス，インク． 新規なｎ２，ｎ４，ｎ７，６−四置換プテリジン−２，４，７−トリアミンおよび２，４，６，７−四置換プテリジン化合物ならびにその合成方法および使用
PT3837970T (pt) 2014-10-15 2023-09-12 Monsanto Technology Llc Genes de tolerância a herbicidas e métodos de uso dos mesmos
CA2974716A1 (en) 2014-11-12 2016-05-19 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
AU2016279062A1 (en) 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
KR102895027B1 (ko) 2015-08-03 2025-12-04 몬산토 테크놀로지 엘엘씨 식물에서 제초제 내성을 위한 방법 및 조성물
US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
BR112018004779A8 (pt) 2015-09-11 2022-08-09 Bayer Cropscience Lp Variantes de hppd e métodos de uso
US11286478B2 (en) 2016-04-19 2022-03-29 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
US11624076B2 (en) 2016-04-21 2023-04-11 BASF Agricultural Solutions Seed US LLC TAL-effector mediated herbicide tolerance
US11732268B2 (en) 2016-06-28 2023-08-22 Monsanto Technology Llc Methods and compositions for use in genome modification in plants
EP3797583A3 (en) 2016-07-29 2021-06-23 Monsanto Technology LLC Methods and compositions for gene expression in plants
WO2018098214A1 (en) 2016-11-23 2018-05-31 Bayer Cropscience Lp Axmi669 and axmi991 toxin genes and methods for their use
MX2019007599A (es) 2016-12-22 2019-12-16 BASF Agricultural Solutions Seed US LLC Uso de cry14 para el control de plagas de nematodos.
UY37570A (es) 2017-01-18 2018-08-31 Bayer Cropscience Lp Uso de bp005 para el control de patógenos de planta
EP3571303A1 (en) 2017-01-18 2019-11-27 Basf Agricultural Solutions Seed Us Llc Bp005 toxin gene and methods for its use
WO2018138385A1 (en) 2017-01-30 2018-08-02 Kws Saat Se Repair template linkage to endonucleases for genome engineering
BR112019018056A2 (pt) 2017-03-07 2020-08-11 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos
BR112020008096A2 (pt) 2017-10-24 2020-11-03 Basf Se método para conferir tolerância a um herbicida e planta de soja transgênica
US11279944B2 (en) 2017-10-24 2022-03-22 BASF Agricultural Solutions Seed US LLC Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean
US11634725B2 (en) 2017-11-03 2023-04-25 University Of Florida Research Foundation, Inc. Methods and compositions for plant pathogen resistance in plants
CN110157727A (zh) 2017-12-21 2019-08-23 中国科学院遗传与发育生物学研究所 植物碱基编辑方法
US12275964B2 (en) 2018-08-22 2025-04-15 The Regents Of The University Of California Variant type V CRISPR/Cas effector polypeptides and methods of use thereof
US11414669B2 (en) 2018-09-06 2022-08-16 Monsanto Technology Llc Compositions and methods for genome editing in planta
WO2020097445A1 (en) 2018-11-09 2020-05-14 Inari Agriculture, Inc. Rna-guided nucleases and dna binding proteins
GB2595606B (en) 2019-03-07 2022-09-21 Univ California CRISPR-Cas effector polypeptides and methods of use thereof
EP4002993A4 (en) 2019-07-30 2024-01-10 Pairwise Plants Services, Inc. MORPHOGENIC REGULATORS AND METHODS OF USE
AU2020367153A1 (en) 2019-10-14 2022-04-28 BASF Agricultural Solutions Seed US LLC Novel insect resistant genes and methods of use
CA3157234A1 (en) 2019-10-14 2021-04-22 BASF Agricultural Solutions Seed US LLC Novel insect resistant genes and methods of use
WO2021113290A1 (en) 2019-12-03 2021-06-10 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
CN113628157A (zh) * 2020-05-06 2021-11-09 德克萨斯大学体系董事会 利用病理图像来表征肿瘤微环境的系统和方法
EP4638759A2 (en) 2022-12-19 2025-10-29 BASF Agricultural Solutions US LLC Insect toxin genes and methods for their use
UY40814A (es) 2023-07-07 2024-12-31 BASF Agricultural Solutions Seed US LLC Uso de genes novedosos para el control de plagas de nematodos
WO2025090606A1 (en) 2023-10-27 2025-05-01 Basf Agricultural Solutions Us Llc Use of novel genes for the control of nematode pests
WO2026010930A1 (en) 2024-07-05 2026-01-08 BASF Agricultural Solutions Seed US LLC Use of axmi277 for the control of rotylenchulus reniformis nematode pests

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422266A (en) 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
NZ214563A (en) 1984-12-31 1991-05-28 Univ Georgia Production of apoaeqorin using recombinant dna
US5225341A (en) 1990-07-19 1993-07-06 The Regents Of The University Of California Biologically safe plant transformation system using a ds transposon
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
GB9216151D0 (en) 1992-07-29 1992-09-09 Ici Plc Containment of plant germplasm
US5360728A (en) 1992-12-01 1994-11-01 Woods Hole Oceanographic Institution (W.H.O.I.) Modified apoaequorin having increased bioluminescent activity
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5491084A (en) 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995007463A1 (en) 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
WO1995021191A1 (en) 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods

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