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PE20190844A1 - Modulacion de transcripcion con arn de direccion a adn generico - Google Patents

Modulacion de transcripcion con arn de direccion a adn generico

Info

Publication number
PE20190844A1
PE20190844A1 PE2019000945A PE2019000945A PE20190844A1 PE 20190844 A1 PE20190844 A1 PE 20190844A1 PE 2019000945 A PE2019000945 A PE 2019000945A PE 2019000945 A PE2019000945 A PE 2019000945A PE 20190844 A1 PE20190844 A1 PE 20190844A1
Authority
PE
Peru
Prior art keywords
transcription
rna
polypeptide
target dna
cell
Prior art date
Application number
PE2019000945A
Other languages
English (en)
Inventor
Martin Jinek
Emmanuelle Charpentier
Krysztof Chylinski
Cate James Harrison Doudna
Wendell Lim
Lei Qi
Original Assignee
Emmanuelle Charpentier
Univ Vienna
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49624232&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PE20190844(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Emmanuelle Charpentier, Univ Vienna, Univ California filed Critical Emmanuelle Charpentier
Publication of PE20190844A1 publication Critical patent/PE20190844A1/es

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    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
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    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
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Abstract

La presente invencion proporciona un metodo de modulacion de la transcripcion especifica del sitio a partir de un ADN diana, que comprende la puesta en contacto del ADN diana con un complejo que comprende: (a) un polipeptido Cas9 que esta fusionado con un polipeptido activador de la transcripcion o un polipeptido represor de la transcripcion, y (b) un ARN de direccion a ADN que comprende: (i) un segmento de direccion a ADN que comprende una secuencia de nucleotidos que es complementaria a una secuencia del ADN diana, y (ii) un segmento de union a proteinas que interactua con dicho polipeptido Cas9, en donde el segmento de union a proteinas comprende dos tramos complementarios de nucleotidos que se hibridan para formar un duplex de ARN bicatenario (ARNbc), en donde dicha puesta en contacto es en una celula ex vivo o en una celula que esta cultivada in vitro. Tambien comprende una composicion, un kit, acidos nucleicos y una celula eucariota modificada geneticamente
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