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WO2008006621A1 - Procédé et dispositif de stockage intermédiaire de microbes - Google Patents

Procédé et dispositif de stockage intermédiaire de microbes Download PDF

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Publication number
WO2008006621A1
WO2008006621A1 PCT/EP2007/006291 EP2007006291W WO2008006621A1 WO 2008006621 A1 WO2008006621 A1 WO 2008006621A1 EP 2007006291 W EP2007006291 W EP 2007006291W WO 2008006621 A1 WO2008006621 A1 WO 2008006621A1
Authority
WO
WIPO (PCT)
Prior art keywords
microbes
carrier medium
container
cooled
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/006291
Other languages
German (de)
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2008006621A1 publication Critical patent/WO2008006621A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

  • the present invention relates to a method and a device for storing microbes.
  • the exhaust gas is passed through at least one reactor containing a porous material which is flooded by a nutrient solution mixed with microbes, whereby the carbon dioxide is converted by the microbes into methane.
  • Hiefur is particularly suitable glycerine or a different carrier medium.
  • the glycerol is filled before mixing with the microbes in an oxygen-free container, which can be achieved by a CO2 fumigation of the empty container in a simple manner.
  • the actual mixing of the carrier medium with the microbes is best done in a MischbehaJ ter, preferably in a ratio of glycerol / microbes of 50: 50, and it is expedient here, the empty Mischbehal ter before by a CO2 fumigation
  • the mixture of carrier medium and microbes is passed through a spiral cooling coil of about 20 to 30 turns, which is conveniently cooled by liquid nitrogen.
  • the cooled mixture of carrier medium and microbes is spent in handy containers, which are suitable for this corresponding plastic bottles. After this filling in the handy container they are placed only in a freezer, which should conveniently have a temperature between about - 18 to -20 0 C. In use then only the z. B. in plastic bottles cooled present only reheated, ie thawed to come to rapid deployment.
  • Particularly suitable microbes for the conversion of carbon dioxide into methane are a Methanosarcinestamm and / or a bacterium Thermoautotrophicum.
  • a Methanosarcinestamm and / or a bacterium Thermoautotrophicum are particularly suitable.
  • the microbial population can also be appropriately preserved and stored.
  • the device for carrying out the method for temporary storage of microbes is characterized in particular by the fact that it comprises separate containers for the carrier medium and the microbes, which are connected to a mixing container, which is followed by a cooling unit through which is the mixture of carrier medium and microbes a filling station and a cooled final storage is fed.
  • a container 1 for the carrier medium and a container 2 are provided for the microbes, which z. B. each have a content of 5 - 10 1.
  • the container 1 has a closable with a ball valve filler neck 3 and a likewise closable with a ball valve connection 4 for the CO2 fumigation, which takes place before the filling of the container 1 with the carrier medium, in this case glycerol.
  • the container 2 which serves to accommodate the microbes also has a closable with a ball valve filler neck 5 and a hose coupling ⁇ to the respective reactor, not shown, which is also closed by a ball valve.
  • the separately arranged containers 1 and 2 are connected via pipes 7 and 8 with interposed ball valves 9 and 10 with a mixing vessel 11, to which in turn a CO2 bottle 12 is connected.
  • a tube 13 with interposed ball valve 14 to a spiral Kuhlschlange 15, which consists of a stainless steel V2A and z. B. 20 turns.
  • the Kuhlschlange 15 is located in a Schutzbehalter 16, in which a cooling by liquid nitrogen takes place, indicated at 17 and can be referred to as a whole as Kuhlaggregat.
  • the method according to the invention for the temporary storage of microbes has the following method steps. First, the container 1 is emptied in the empty state via the terminal 4 with CO2 to get its interior oxygen-free. Then, the container 1 is filled via the filler neck 3 with glycerol. According to the amount of glycerol, the container 2 is then filled with microbes, either "fresh product” via the filler neck 5 or via the hose coupling 6 "used goods" from a reactor, in both cases, respectively, the ball valves provided in a known manner are pressing ..
  • the mixture then flows through the spiral cooling coil 15, which was pre-cooled by means of the nitrogen cooling 17, namely so far that a visible ice formation on the cooling coil 15 is present.
  • the ball valve 19 is opened to the filling station 18 ⁇ and spent the cooled mixture in suitable 2 1 plastic bottles 20. Finally, the frozen microbes are stored in the freezer 21 at - 18 to -20 0 C.
  • the method according to the invention offers a number of advantages for this, namely a largely 02-free process; a good and fast germ preservative preservation;

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Packages (AREA)

Abstract

L'invention concerne un procédé et un dispositif correspondant de stockage intermédiaire de microbes. Selon l'invention, les microbes sont mélangés à un fluide porteur et le mélange est ensuite stocké de façon réfrigérée.
PCT/EP2007/006291 2006-07-14 2007-07-16 Procédé et dispositif de stockage intermédiaire de microbes Ceased WO2008006621A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006033098.6 2006-07-14
DE102006033098A DE102006033098A1 (de) 2006-07-14 2006-07-14 Verfahren und Einrichtung zum Zwischenlagern von Mikroben

Publications (1)

Publication Number Publication Date
WO2008006621A1 true WO2008006621A1 (fr) 2008-01-17

Family

ID=38521156

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/006291 Ceased WO2008006621A1 (fr) 2006-07-14 2007-07-16 Procédé et dispositif de stockage intermédiaire de microbes

Country Status (2)

Country Link
DE (1) DE102006033098A1 (fr)
WO (1) WO2008006621A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012003113A1 (de) * 2012-02-16 2013-08-22 Forschungszentrum Jülich GmbH Probeentnahmevorrichtung und Verfahren zur Entnahme einer Probe aus einem Bioreaktor

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2208451A1 (de) * 1971-02-26 1972-09-14 Leo Kline Verfahren zum Zuchten von Sauerteig bakterien
EP0006671A2 (fr) * 1978-07-03 1980-01-09 Firma Dr.Ing. Hans Müller Procédé de conservation de microorganismes vivants
JPH0265776A (ja) * 1988-08-29 1990-03-06 Agency Of Ind Science & Technol 酵母細胞の新規凍結保存法
WO1993011220A1 (fr) * 1991-12-03 1993-06-10 Mastavac Limited Preparations medicales
US5759764A (en) * 1995-03-08 1998-06-02 Celox Laboratories, Inc. Cryopreservation solution
WO2003020874A2 (fr) * 2001-09-06 2003-03-13 I.M.T Interface Multigrad Technology Ltd. Procede perfectionne permettant la congelation de cellules viables
WO2005072523A2 (fr) * 2004-02-02 2005-08-11 I.M.T. Interface Multigrad Technology Ltd. Materiau biologique et procedes et solutions de conservation de celui-ci
EP1647186A1 (fr) * 2004-10-12 2006-04-19 Nipro Corporation Liquide de conservation de cellules

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3228838A (en) * 1959-04-23 1966-01-11 Union Carbide Corp Preservation of biological substances
US3753357A (en) * 1970-12-14 1973-08-21 Ovitron Res Corp Method and apparatus for the preservation of cells and tissues
DE2557870A1 (de) * 1975-12-22 1977-06-23 Linde Ag Verfahren und vorrichtung zum tiefgefrieren von biologischen substanzen
EP0302968A1 (fr) * 1987-08-10 1989-02-15 Michigan Biotechnology Institute Inoculats et procédé de préparation de biométhane
JP3015133B2 (ja) * 1991-04-23 2000-03-06 キヤノン株式会社 印刷制御装置及び方法
US5342524A (en) * 1991-05-24 1994-08-30 Gaddy James L Performance of anaerobic digesters
DE10117205A1 (de) * 2001-04-06 2002-10-17 Martin Winkemann Vorrichtung zur Vorbereitung von Zellen für die Kryokonservierung

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2208451A1 (de) * 1971-02-26 1972-09-14 Leo Kline Verfahren zum Zuchten von Sauerteig bakterien
EP0006671A2 (fr) * 1978-07-03 1980-01-09 Firma Dr.Ing. Hans Müller Procédé de conservation de microorganismes vivants
JPH0265776A (ja) * 1988-08-29 1990-03-06 Agency Of Ind Science & Technol 酵母細胞の新規凍結保存法
WO1993011220A1 (fr) * 1991-12-03 1993-06-10 Mastavac Limited Preparations medicales
US5759764A (en) * 1995-03-08 1998-06-02 Celox Laboratories, Inc. Cryopreservation solution
WO2003020874A2 (fr) * 2001-09-06 2003-03-13 I.M.T Interface Multigrad Technology Ltd. Procede perfectionne permettant la congelation de cellules viables
WO2005072523A2 (fr) * 2004-02-02 2005-08-11 I.M.T. Interface Multigrad Technology Ltd. Materiau biologique et procedes et solutions de conservation de celui-ci
EP1647186A1 (fr) * 2004-10-12 2006-04-19 Nipro Corporation Liquide de conservation de cellules

Also Published As

Publication number Publication date
DE102006033098A1 (de) 2008-01-24

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