[go: up one dir, main page]

RU2017132988A - Синтетические транзитные пептиды хлоропластов - Google Patents

Синтетические транзитные пептиды хлоропластов Download PDF

Info

Publication number
RU2017132988A
RU2017132988A RU2017132988A RU2017132988A RU2017132988A RU 2017132988 A RU2017132988 A RU 2017132988A RU 2017132988 A RU2017132988 A RU 2017132988A RU 2017132988 A RU2017132988 A RU 2017132988A RU 2017132988 A RU2017132988 A RU 2017132988A
Authority
RU
Russia
Prior art keywords
nucleic acid
acid molecule
plant material
plant
peptide
Prior art date
Application number
RU2017132988A
Other languages
English (en)
Other versions
RU2017132988A3 (ru
RU2720973C2 (ru
Inventor
Джастин М. ЛИРА
Роберт М. ЧИККИЛЛО
Карла Йеркс
Эндрю Э. РОБИНСОН
Original Assignee
ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи filed Critical ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи
Publication of RU2017132988A3 publication Critical patent/RU2017132988A3/ru
Publication of RU2017132988A publication Critical patent/RU2017132988A/ru
Application granted granted Critical
Publication of RU2720973C2 publication Critical patent/RU2720973C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Insects & Arthropods (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Claims (43)

1. Выделенная молекула нуклеиновой кислоты, содержащая:
нуклеотидную последовательность, кодирующую синтетический транзитный пептид хлоропласта (CTP), состоящий из аминокислотной последовательности, которая получена при выравнивании аминокислотных остатков ферментов 5-энолпирувилшикимат-3-фосфат-синтазы (EPSPS), где каждый из аминокислотных остатков CTP соответствует аминокислотному остатку в соответствующем положении при выравнивании полипептидов, выбранных из группы, состоящей из SEQ ID NO: 1-10, где синтетический CTP по меньшей мере на 80% идентичен транзитному пептиду хлоропласта SEQ ID NO: 11; и
гетерологичную нуклеотидную последовательность, кодирующую представляющую интерес пептид, функционально связанный с синтетической CTP-кодирующей нуклеотидной последовательностью,
где молекула нуклеиновой кислоты кодирует химерный полипептид, содержащий представляющий интерес пептид и синтетический CTP.
2. Выделенная молекула нуклеиновой кислоты по п.1, в которой синтетический CTP состоит из аминокислотной последовательности, которая получена при выравнивании ферментов 5-энолпирувилшикимат-3-фосфат-синтазы (EPSPS), выделенных из видов, выбранных из группы, состоящей из: Brassica sp., Glycine sp., Arabidopsis sp. и Duneila sp.
3. Выделенная молекула нуклеиновой кислоты по п.1, в которой синтетический CTP состоит из 61 C-концевой аминокислоты CTP SEQ ID NO: 11.
4. Выделенная молекула нуклеиновой кислоты по п.1, в которой синтетический CTP по меньшей мере на 90% идентичен CTP SEQ ID NO: 11.
5. Выделенная молекула нуклеиновой кислоты по п.4, в которой синтетический CTP по меньшей мере на 95% идентичен CTP SEQ ID NO: 11.
6. Выделенная молекула нуклеиновой кислоты по п.5, в которой синтетический CTP по меньшей мере на 98% идентичен CTP SEQ ID NO: 11.
7. Выделенная молекула нуклеиновой кислоты по п.1, в которой нуклеотидная последовательность, кодирующая синтетический CTP, остается связанной с нуклеиновой кислотой SEQ ID NO: 12 после гибридизации в 5-6 × SSC-буфере при 65-70 °C в течение 16-20 часов, двух промываний в 2 × SSC-буфере при комнатной температуре в течение 5-20 минут каждое, и двух промываний в 1 × SSC-буфере при 55-70 °C в течение 30 минут каждое.
8. Выделенная молекула нуклеиновой кислоты по п.1, дополнительно содержащая по меньшей мере одну дополнительную нуклеотидную последовательность (последовательности), каждая из которых кодирует CTP, где дополнительная нуклеотидная последовательность (последовательности) функционально связана с нуклеотидной последовательностью, кодирующей представляющий интерес пептид.
9. Выделенная молекула нуклеиновой кислоты по п.8, в которой дополнительная нуклеотидная последовательность происходит от организма, выбранного из группы, состоящей из прокариот, низших фотосинтезирующих эукариот и хлорофитов.
10. Выделенная молекула нуклеиновой кислоты по п.8, в которой синтетическая CTP-кодирующая последовательность и нуклеотидная последовательность, кодирующая представляющий интерес пептид, функционально связаны с одной или несколькими регуляторными последовательностями.
11. Молекула нуклеиновой кислоты по п.1, отличающаяся тем, что молекула представляет собой растительный вектор экспрессии.
12. Химерный полипептид, кодируемый молекулой нуклеиновой кислоты по п.1.
13. Химерный полипептид по п.12, в котором представляющий интерес пептид нацелен на пластид в пластид-содержащей клетке.
14. Химерный полипептид по п.13, в котором синтетический CTP удаляется, когда представляющий интерес пептид нацелен на пластид.
15. Химерный полипептид по п.12, в котором представляющим интерес пептидом является флуоресцентный пептид.
16. Химерный полипептид по п.12, в котором представляющий интерес пептид является маркером устойчивости к гербициду, выбранным из группы, состоящей из: ацетолактат-синтазы (АЛС), мутантной АЛС, предшественников АЛС, 5-энолпирувилшикимат-3-фосфат-синтазы (EPSPS), CP4 EPSPS и EPSPS класса III.
17. Химерный полипептид по п.12, в котором представляющий интерес пептид представляет собой DGT-28 или Cry2Aa.
18. Трансгенный растительный материал, содержащий молекулу нуклеиновой кислоты по п.1.
19. Трансгенный растительный материал по п.18, отличающийся тем, что растительный материал выбран из группы, состоящей из растительной клетки, растительной ткани, культуры растительных тканей, каллусной культуры и части растения.
20. Трансгенный растительный материал по п.18, отличающийся тем, что растительный материал представляет собой целое растение.
21. Трансгенный растительный материал по п.18, в котором молекула нуклеиновой кислоты стабильно интегрирована в геном клетки из растительного материала.
22. Способ получения трансгенного растительного материала, включающий трансформацию растительного материала молекулой нуклеиновой кислоты по п.1.
23. Способ получения трансгенного растения, включающий регенерацию трансгенного растения из трансгенного растительного материала по п.18.
24. Выделенная молекула нуклеиновой кислоты, содержащая полученный из EPSPS агент для нацеливания полипептида на хлоропласт и нуклеотидную последовательность, кодирующую представляющий интерес пептид, функционально связанный с полученным из EPSPS агентом для нацеливания полипептида на хлоропласт, где молекула нуклеиновой кислоты кодирует химерный полипептид, содержащий представляющий интерес пептид, и пептид, кодируемый полученным из EPSPS агентом для нацеливания полипептида на хлоропласт.
25. Выделенная молекула нуклеиновой кислоты по п.24, где полученный из EPSPS агент для нацеливания полипептида на хлоропласт, кодирует пептид, состоящий из аминокислотной последовательности, которая по меньшей мере на 90% идентична SEQ ID NO: 11.
26. Выделенная молекула нуклеиновой кислоты по п.24, где полученный из EPSPS агент для нацеливания полипептида на хлоропласт, кодирует пептид, состоящий из аминокислотной последовательности, которая по меньшей мере на 95% идентична SEQ ID NO: 11.
27. Молекула нуклеиновой кислоты по п.24, отличающаяся тем, что молекула представляет собой растительный вектор экспрессии.
28. Химерный полипептид, кодируемый молекулой нуклеиновой кислоты по п.24.
29. Химерный полипептид по п.28, в котором представляющий интерес пептид представляет собой DGT-28 или Cry2Aa.
30. Трансгенный растительный материал, содержащий молекулу нуклеиновой кислоты по п.24.
31. Трансгенный растительный материал по п.30, отличающийся тем, что растительный материал выбран из группы, состоящей из растительной клетки, растительной ткани, культуры растительных тканей, каллусной культуры и части растения.
32. Трансгенный растительный материал по п.30, отличающийся тем, что растительный материал представляет собой целое растение.
33. Трансгенный растительный материал по п.30, в котором молекула нуклеиновой кислоты стабильно интегрирована в геном клетки из растительного материала.
34. Способ получения трансгенного растительного материала, включающий трансформацию растительного материала молекулой нуклеиновой кислоты по п.24.
35. Способ получения трансгенного растения, включающий регенерацию трансгенного растения из трансгенного растительного материала по п.31.
36. Способ по п.35, в котором растительный материал выбран из группы, состоящей из растительной клетки, растительной ткани, культуры растительных тканей, каллусной культуры, части растения и целого растения.
37. Трансгенный растительный материал, полученный способом по п.35.
38. Трансгенное растение, регенерированное из трансгенного растительного материала по п.37.
39. Растительный материал по п.30, отличающийся тем, что растительный материал представляет собой растительную клетку, которая не может быть регенерирована для получения растения.
40. Способ по п.34, отличающийся тем, что растительный материал является растительной клеткой, которая не может быть регенерирована для получения растения.
RU2017132988A 2012-02-01 2013-02-01 Синтетические транзитные пептиды хлоропластов RU2720973C2 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261593555P 2012-02-01 2012-02-01
US61/593,555 2012-02-01
US201261625222P 2012-04-17 2012-04-17
US61/625,222 2012-04-17

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
RU2014135324A Division RU2632648C2 (ru) 2012-02-01 2013-02-01 Синтетические транзитные пептиды хлоропластов

Publications (3)

Publication Number Publication Date
RU2017132988A3 RU2017132988A3 (ru) 2019-02-07
RU2017132988A true RU2017132988A (ru) 2019-02-07
RU2720973C2 RU2720973C2 (ru) 2020-05-15

Family

ID=48904120

Family Applications (8)

Application Number Title Priority Date Filing Date
RU2014135335A RU2626535C2 (ru) 2012-02-01 2013-02-01 Синтетические происходящие из brassica транзитные пептиды хлоропластов
RU2017133853A RU2721122C2 (ru) 2012-02-01 2013-02-01 Новый класс генов устойчивости к глифосату
RU2014135442A RU2636036C2 (ru) 2012-02-01 2013-02-01 Транзитный пептид хлоропластов
RU2014135340A RU2636037C2 (ru) 2012-02-01 2013-02-01 Устойчивые к глифосату растения и способы, связанные с ними
RU2014135324A RU2632648C2 (ru) 2012-02-01 2013-02-01 Синтетические транзитные пептиды хлоропластов
RU2014135466A RU2634411C2 (ru) 2012-02-01 2013-02-01 Новый класс генов устойчивости к глифосату
RU2017132988A RU2720973C2 (ru) 2012-02-01 2013-02-01 Синтетические транзитные пептиды хлоропластов
RU2014135403A RU2627169C2 (ru) 2012-02-01 2013-02-01 Устойчивые к глифосату растения и способы, связанные с ними

Family Applications Before (6)

Application Number Title Priority Date Filing Date
RU2014135335A RU2626535C2 (ru) 2012-02-01 2013-02-01 Синтетические происходящие из brassica транзитные пептиды хлоропластов
RU2017133853A RU2721122C2 (ru) 2012-02-01 2013-02-01 Новый класс генов устойчивости к глифосату
RU2014135442A RU2636036C2 (ru) 2012-02-01 2013-02-01 Транзитный пептид хлоропластов
RU2014135340A RU2636037C2 (ru) 2012-02-01 2013-02-01 Устойчивые к глифосату растения и способы, связанные с ними
RU2014135324A RU2632648C2 (ru) 2012-02-01 2013-02-01 Синтетические транзитные пептиды хлоропластов
RU2014135466A RU2634411C2 (ru) 2012-02-01 2013-02-01 Новый класс генов устойчивости к глифосату

Family Applications After (1)

Application Number Title Priority Date Filing Date
RU2014135403A RU2627169C2 (ru) 2012-02-01 2013-02-01 Устойчивые к глифосату растения и способы, связанные с ними

Country Status (24)

Country Link
US (13) US9873883B2 (ru)
EP (9) EP3219200B1 (ru)
JP (8) JP6349261B2 (ru)
KR (6) KR20140132352A (ru)
CN (11) CN104219949B (ru)
AP (4) AP2014007886A0 (ru)
AR (7) AR090417A1 (ru)
AU (11) AU2013205521B2 (ru)
BR (8) BR102013002599B1 (ru)
CA (6) CA2863201A1 (ru)
CL (6) CL2014002018A1 (ru)
CO (6) CO7051012A2 (ru)
ES (1) ES2716481T3 (ru)
HU (1) HUE042672T2 (ru)
IL (5) IL233858A (ru)
MX (9) MX343876B (ru)
NZ (5) NZ628989A (ru)
PH (4) PH12014501728A1 (ru)
RU (8) RU2626535C2 (ru)
TW (3) TW201335365A (ru)
UA (5) UA116980C2 (ru)
UY (3) UY34606A (ru)
WO (6) WO2013116773A1 (ru)
ZA (6) ZA201405891B (ru)

Families Citing this family (158)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102013002599B1 (pt) * 2012-02-01 2022-04-05 Dow Agrosciences Llc Molécula de ácido nucleico isolada ou recombinante, método para gerar uma célula vegetal tolerante a glifosato e usos da mesma
AU2013205557B2 (en) * 2012-04-17 2016-04-21 Corteva Agriscience Llc Synthetic brassica-derived chloroplast transit peptides
JP6775953B2 (ja) 2012-09-07 2020-10-28 ダウ アグロサイエンシィズ エルエルシー Ｆａｄ３性能座および標的化切断を誘導可能である対応する標的部位特異的結合タンパク質
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
UA121197C2 (uk) 2013-04-05 2020-04-27 Доу Агросайенсіс Ллс Нуклеаза "цинкові пальці" для модифікацїї гена ahas та спосіб її використання
CA3223359A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2014329590B2 (en) 2013-10-04 2017-10-19 Dow Agrosciences Llc Zea mays metallothionein-like regulatory elements and uses thereof
BR102014025499A2 (pt) 2013-10-15 2015-09-29 Dow Agrosciences Llc elementos regulatórios de zea mays e uso dos mesmos
JP2016535044A (ja) * 2013-10-29 2016-11-10 ハイポ−ストリーム・リミテッド 次亜塩素酸ナトリウムを含む抗炎症溶液
CN105828615B (zh) 2013-12-20 2020-05-22 美国陶氏益农公司 协同除草杂草防治
AR100785A1 (es) 2014-06-09 2016-11-02 Dow Agrosciences Llc Control herbicida de maleza a partir de combinaciones de fluroxipir e inhibidores de als
AP2016009601A0 (en) * 2014-06-20 2016-12-31 Dow Agrosciences Llc Vegetative insecticidal proteins useful for control of insect pests
KR101788673B1 (ko) * 2014-06-24 2017-11-15 싸이퍼롬, 인코퍼레이티드 핵산염기서열 보안 방법, 장치 및 이를 저장한 기록매체
AU2015286205A1 (en) * 2014-07-08 2016-12-15 MINISTER FOR AGRICULTURE, FOOD AND FISHERIES acting through THE SOUTH AUSTRALIAN RESEARCH AND DEVELOPMENT INSTITUTE (ABN 53 763 159 658) A BODY CORPORATE PURSUANT TO THE ADMINISTRATIVE ARRANGEMENTS ACT 1994 (SOUTH AUSTRALIA) Herbicide tolerant plants
IL315468A (en) 2014-09-17 2024-11-01 Spogen Biotech Inc Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
AU2015333935B2 (en) 2014-10-15 2019-12-05 Monsanto Technology Llc Herbicide tolerance genes and methods of use thereof
RU2017122359A (ru) * 2014-12-30 2019-01-31 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Модифицированные токсины cryca, применимые для борьбы с насекомыми-вредителями
UA126544C2 (uk) 2015-01-15 2022-11-02 Піонір Хай-Бред Інтернешнл, Інк. Рекомбінантний полінуклеотид, що кодує інсектицидний поліпептид, та спосіб його застосування
MX376048B (es) 2015-03-11 2025-03-07 Corteva Agriscience Llc Combinaciones insecticidas de pip-72 y métodos de uso.
BR112017021703B1 (pt) 2015-04-15 2023-11-28 Corteva Agriscience Llc Promotor de planta para expressão de transgene
KR20170136549A (ko) 2015-04-15 2017-12-11 다우 아그로사이언시즈 엘엘씨 트랜스젠 발현을 위한 식물 프로모터
KR20180038048A (ko) * 2015-08-20 2018-04-13 다우 아그로사이언시즈 엘엘씨 엽록체 전이 펩티드
WO2017034853A1 (en) * 2015-08-25 2017-03-02 Dow Agrosciences Llc Irdig17912 insecticidal cry toxins
CN105132546A (zh) * 2015-08-31 2015-12-09 四川省农业科学院作物研究所 一种养分高效利用转基因玉米材料快速鉴定的方法
WO2017044372A1 (en) * 2015-09-11 2017-03-16 AgBiome, Inc. Chloroplast transit peptides and methods of their use
TW201718861A (zh) 2015-09-22 2017-06-01 道禮責任有限公司 用於轉殖基因表現之植物啟動子及３’ｕｔｒ
TW201718862A (zh) 2015-09-22 2017-06-01 Dow Agrosciences Llc 用於轉殖基因表現之植物啟動子及３’ｕｔｒ
US10655141B2 (en) * 2015-09-30 2020-05-19 Pioneer Hi-Bred International, Inc. Plant EPSP synthases and methods of use
EP3365451B1 (en) 2015-10-22 2020-06-24 Dow Agrosciences LLC Plant promoter for transgene expression
US20170121726A1 (en) 2015-11-04 2017-05-04 Dow Agrosciences Llc Plant promoter for transgene expression
CN109640649A (zh) 2016-03-16 2019-04-16 斯波根生物技术公司 使用游离酶和过表达酶的微生物促进植物健康的方法
MX2018012613A (es) * 2016-04-19 2019-08-01 Dow Agrosciences Llc Combinacion de cuatro toxinas de proteinas vip y cry para el manejo de plagas de insectos en plantas.
EP3960863B1 (en) 2016-05-04 2024-11-13 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EA201892517A1 (ru) * 2016-05-20 2019-05-31 Басф Агро Б.В. Двойные транзитные пептиды для нацеливания полипептидов
WO2018017606A1 (en) * 2016-07-18 2018-01-25 Cell Idx, Inc. Antigen-coupled hybridization reagents
CA3035229A1 (en) * 2016-09-02 2018-03-08 North Carolina State University Methods and compositions for modification of plastid genomes
EP3519574B1 (en) 2016-10-03 2022-01-19 Corteva Agriscience LLC Plant promoter for transgene expression
CN109996436B (zh) 2016-10-03 2023-09-29 科迪华农业科技有限责任公司 用于转基因表达的植物启动子
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
CN106498063B (zh) * 2016-11-08 2019-12-10 云南省烟草农业科学研究院 一种分子标记辅助选择定向改良烟草黑胫病抗性的育种方法
CN106967746A (zh) * 2017-02-04 2017-07-21 深圳万智联合科技有限公司 一种快速获得抗除草剂的转基因高粱纯合植株的方法
WO2018183050A2 (en) * 2017-03-30 2018-10-04 Pioneer Hi-Bred International, Inc. Improved plant epsp synthases and methods of use
EP3654763A4 (en) 2017-06-28 2021-06-09 Dow Agrosciences LLC PLANT PROMOTER FOR TRANSGENIC EXPRESSION
CN107502621B (zh) * 2017-09-22 2021-04-13 福建农林大学 一种快速检测体内dna末端连接的方法
BR112020013929A2 (pt) * 2018-01-17 2020-12-01 Basf Se plantas ou partes da planta, semente, células vegetais, produto vegetal, progênie ou planta descendente, métodos para controlar ervas daninhas, para produzir uma planta e para produzir uma planta descendente, molécula de ácido nucleico, cassete de expressão, vetor, polipeptídeo, método para produzir um produto vegetal e produto vegetal
CN110386966A (zh) * 2018-04-23 2019-10-29 中国科学院大连化学物理研究所 一种含有叶绿体转运肽的基因序列的应用
CN110616203B (zh) 2018-06-04 2022-04-01 青岛清原化合物有限公司 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用
JP2020146307A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146311A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146317A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146306A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146301A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146318A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146302A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146314A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146297A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146303A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146305A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146310A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146315A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146316A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146312A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146304A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146313A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146299A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146308A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146300A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146298A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
CR20210478A (es) 2019-03-19 2021-11-05 Bayer Cropscience Lp Proteínas de fusión, bacterias recombinantes, y fragmentos de exosporio para el control de plagas y salud vegetal
CA3136356A1 (en) 2019-04-12 2020-10-15 Ecolab Usa Inc. Antimicrobial multi-purpose cleaner and methods of making and using the same
CN110095613A (zh) * 2019-05-27 2019-08-06 武汉上成生物科技有限公司 一种快速检测转基因植物中cp4-epsps的方法
EP4002993A4 (en) 2019-07-30 2024-01-10 Pairwise Plants Services, Inc. MORPHOGENIC REGULATORS AND METHODS OF USE
WO2021081367A1 (en) 2019-10-23 2021-04-29 Pairwise Plants Services, Inc. Compositions and methods for rna-templated editing in plants
KR20220110739A (ko) 2019-10-30 2022-08-09 페어와이즈 플랜츠 서비시즈, 인크. 유형 v crispr-cas 염기 편집제 및 그의 사용 방법
KR102088178B1 (ko) * 2019-11-01 2020-03-12 경희대학교 산학협력단 엽록체 내 스트로마를 표적하는 트랜지트 펩티드 및 이의 용도
KR20220110748A (ko) 2019-11-05 2022-08-09 페어와이즈 플랜츠 서비시즈, 인크. 대립유전자의 rna-코딩된 dna-대체를 위한 조성물 및 방법
WO2021113788A1 (en) 2019-12-06 2021-06-10 Pairwise Plants Services, Inc. Recruitment methods and compounds, compositions and systems for recruitment
CN111139228B (zh) * 2019-12-31 2021-04-06 华中农业大学 一种抗草甘膦植物epsps酶双突变体及其克隆、表达与应用
CN115380111A (zh) 2020-01-30 2022-11-22 成对植物服务股份有限公司 用于碱基多样化的组合物、系统和方法
AR121242A1 (es) 2020-01-31 2022-05-04 Pairwise Plants Services Inc Supresión de la respuesta de escape a la sombra en plantas
WO2021158798A1 (en) 2020-02-04 2021-08-12 Pairwise Plants Services, Inc. Thornless / prickleless rubus plants
WO2021168288A1 (en) 2020-02-21 2021-08-26 Pairwise Plants Services, Inc. Improved resistance to soybean cyst nematode through gene editing
WO2021188526A1 (en) 2020-03-16 2021-09-23 Pairwise Plants Services, Inc. Natural guide architectures and methods of making and using the same
US11999946B2 (en) 2020-03-26 2024-06-04 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US11882808B2 (en) 2020-03-27 2024-01-30 Pairwise Plants Services, Inc. Methods for improving resistance to soybean rust
WO2021207148A1 (en) 2020-04-06 2021-10-14 Pairwise Plants Services, Inc. Methods and compositions for increasing resistance to ear rot and stem rot disease in maize
CN119431532A (zh) 2020-04-16 2025-02-14 成对植物服务股份有限公司 控制分生组织大小以改良作物的方法
WO2021247477A1 (en) 2020-06-02 2021-12-09 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
CA3186972A1 (en) 2020-06-17 2021-12-23 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
CA3187135A1 (en) 2020-06-30 2022-01-06 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
WO2022026379A1 (en) * 2020-07-31 2022-02-03 Inari Agriculture Technology, Inc. Expedited breeding of transgenic crop plants by genome editing
BR112023001774A2 (pt) * 2020-07-31 2023-03-07 Inari Agriculture Tech Inc Genoma de planta transgênica editado, célula de planta transgênica, planta transgênica, parte de planta transgênica, método para obter uma população avolumada de sementes endogâmicas para produção de sementes comerciais, método de obtenção de semente de cultura híbrida, molécula de dna, produto de planta transgênica processado, amostra biológica, marcador de ácido nucleico adaptado para detecção de dna genômico ou seus fragmentos, método de detecção do genoma de planta transgênica editado, método de obtenção de um genoma de planta transgênica editado compreendendo um lócus transgênico modificado, método de excisão de um lócus transgênico modificado ou sua porção a partir de um genoma de planta transgênica editado, método de obtenção de uma linha de reprodução de plantas, método para obter germoplasma de planta transgênica endogâmico contendo diferentes traços transgênicos
BR112023001759A2 (pt) * 2020-07-31 2023-03-07 Inari Agriculture Tech Inc Método de produção de uma planta, planta, genoma de planta, método para obter a planta, método de obtenção de semente, dna, marcador de ácido nucleico, amostra biológica, método de identificação da planta, método de intensificação da funcionalidade de um lócus, método para obter uma população avolumada de sementes
CA3188282A1 (en) * 2020-07-31 2022-02-03 Inari Agriculture Technology, Inc. Expedited breeding of transgenic crop plants by genome editing
CN111996205A (zh) * 2020-08-10 2020-11-27 深圳润康生态环境股份有限公司 几丁质酶基因、几丁质酶及其制备方法和应用
IL300823A (en) 2020-08-28 2023-04-01 Pairwise Plants Services Inc Transgenic CRISPR-CAS proteins and methods of using them
EP4204427A4 (en) 2020-09-05 2025-03-05 The Board of Trustees of the University of Illinois PHOSPHONATE PRODUCTS AND PROCESSES
CN112266419B (zh) * 2020-10-29 2023-02-03 中国科学院微生物研究所 一种人工合成的抗除草剂蛋白
US20220145334A1 (en) 2020-11-06 2022-05-12 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2022109448A1 (en) 2020-11-23 2022-05-27 Pairwise Plants Services, Inc. Agrobacterium rhizogenes and methods of transforming cells
MX2023009468A (es) 2021-02-11 2024-01-31 Pairwise Plants Services Inc Metodos y composiciones para modificar niveles de citoquinina oxidasa en plantas.
CN117203227A (zh) 2021-02-25 2023-12-08 成对植物服务股份有限公司 用于修饰植物中根结构的方法和组合物
US20220403475A1 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
AR126172A1 (es) 2021-06-17 2023-09-27 Pairwise Plants Services Inc Modificación de factores de transcripción de la familia de factores reguladores del crecimiento en soja
UY39827A (es) 2021-06-24 2023-01-31 Pairwise Plants Services Inc Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento
WO2023278651A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
CN118076742A (zh) 2021-08-12 2024-05-24 成对植物服务股份有限公司 修饰油菜素内酯受体基因以改善产量性状
UY39902A (es) 2021-08-17 2023-03-31 Pairwise Plants Services Inc Métodos y composiciones para modificar genes de histidina quinasa receptores de citoquinina en plant
MX2024002276A (es) 2021-08-30 2024-03-07 Pairwise Plants Services Inc Modificacion de genes de peptidasa de union a ubiquitinaen plantas para mejorar rasgos de rendimiento.
AR126938A1 (es) 2021-09-02 2023-11-29 Pairwise Plants Services Inc Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento
WO2023049720A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Methods and compositions for reducing pod shatter in canola
US20230266293A1 (en) 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
WO2023060028A1 (en) 2021-10-04 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
UY39970A (es) 2021-10-07 2023-04-28 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de la flor y el rendimiento de semillas
MX2024005844A (es) * 2021-11-16 2024-05-27 Pioneer Hi Bred Int Evento de maiz das-01131-3 y metodos para la deteccion de este.
AR127904A1 (es) 2021-12-09 2024-03-06 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
WO2023114750A1 (en) 2021-12-13 2023-06-22 Pairwise Plants Services, Inc. Model editing systems and methods relating to the same
CN114196660B (zh) * 2021-12-15 2022-11-08 中国农业科学院油料作物研究所 油菜fc2亚铁螯合酶基因在提高油菜产量中的应用
US20250146008A1 (en) 2022-01-06 2025-05-08 Pairwise Plants Services, Inc. Methods and compositions for trichome removal
CN114085277B (zh) * 2022-01-19 2022-04-19 北京市农林科学院 一种将目的蛋白定位于叶绿体的方法及其应用
CN114085278B (zh) * 2022-01-20 2022-04-26 北京市农林科学院 Lsa25711转运肽在叶绿体遗传转化中的应用
UY40132A (es) 2022-01-31 2023-08-31 Pairwise Plants Services Inc Supresión de la respuesta de evitación de la sombra en las plantas
EP4486880A1 (en) 2022-02-28 2025-01-08 Pairwise Plants Services, Inc. Engineered crispr-cas effector proteins and methods of use thereof
US20240327858A1 (en) 2022-03-02 2024-10-03 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
US20260043040A1 (en) 2022-03-31 2026-02-12 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
CA3247405A1 (en) 2022-04-07 2023-10-12 Pairwise Plants Services, Inc. METHODS AND COMPOSITIONS FOR IMPROVING RESISTANCE TO FUSARIA BROAD OF THE SPINE
CA3249359A1 (en) 2022-04-21 2023-10-26 Pairwise Plants Services, Inc. PROCESSES AND COMPOSITIONS FOR IMPROVING PERFORMANCE CHARACTERISTICS
UY40250A (es) 2022-05-02 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para mejorar el rendimiento y la resistencia a enfermedades
UY40255A (es) 2022-05-05 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectur radicular y/o mejorar los rangos de rendimient
UY40326A (es) 2022-06-27 2023-12-29 Pairwise Plants Services Inc Métodos y composiciones para modificar el escape a la sombra en plantas
WO2024006791A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
US20240002873A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN119768514A (zh) 2022-08-04 2025-04-04 成对植物服务股份有限公司 用于改善产量性状的方法和组合物
AR130164A1 (es) 2022-08-11 2024-11-13 Pairwise Plants Services Inc Métodos y composiciones para controlar el tamaño del meristema para la mejora de los cultivos
EP4584282A1 (en) 2022-09-08 2025-07-16 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
CN115623986B (zh) * 2022-10-27 2023-12-15 天津农学院 一种鉴定芹菜愈伤组织完全胚性化的方法
US20240200102A1 (en) 2022-12-16 2024-06-20 Pairwise Plants Services, Inc. Fusion proteins comprising an intein polypeptide and methods of use thereof
AU2023413056A1 (en) 2022-12-21 2025-06-12 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
CN120603841A (zh) 2023-02-16 2025-09-05 成对植物服务股份有限公司 用于改变植物避荫性的方法和组合物
WO2024182746A1 (en) 2023-03-01 2024-09-06 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
AR132019A1 (es) 2023-03-02 2025-05-21 Pairwise Plants Services Inc Métodos y composiciones para modificar la evitación de la sombra en plantas
UY40664A (es) 2023-03-09 2024-10-15 Pairwise Plants Services Inc Modificación de genes de la vía de señalización de brasinoesteroide para mejorar rasgos de rendimien
AU2024248777A1 (en) 2023-03-30 2025-10-02 Pairwise Plants Services, Inc. Methods and compositions for reducing thorns or prickles in plants
UY40746A (es) 2023-05-18 2024-12-13 Pairwise Plants Services Inc Métodos y composiciones para mejorar las características de rendimiento de las plantas
CN116789780B (zh) * 2023-05-26 2023-12-01 中国农业科学院生物技术研究所 用于抗草甘膦除草剂基因的叶绿体转运肽及其应用
AR133164A1 (es) 2023-07-18 2025-09-03 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectura radicular en plantas
AR133310A1 (es) 2023-07-27 2025-09-17 Pairwise Plants Services Inc Métodos y composiciones para modificar rasgos de rendimiento de las plantas
AR133901A1 (es) 2023-09-21 2025-11-12 Pairwise Plants Services Inc Plantas de frambuesa negra de floración temprana con características mejoradas
US20250109389A1 (en) 2023-09-26 2025-04-03 Pairwise Plants Services, Inc. Fusion proteins, compositions comprising the same, and methods of use thereof
WO2025080537A1 (en) 2023-10-09 2025-04-17 Pairwise Plants Services, Inc. Circular permutants of cas12a
WO2025080600A1 (en) 2023-10-11 2025-04-17 Pairwise Plants Services, Inc. Methods and compositions for improving crop yield traits
WO2025137408A1 (en) 2023-12-21 2025-06-26 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2025178902A1 (en) 2024-02-22 2025-08-28 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A
WO2026015427A1 (en) 2024-07-08 2026-01-15 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of cas12a
WO2026019870A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered crispr/cas12a proteins and methods of use thereof
WO2026019871A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered cytidine deaminases
CN118547001A (zh) * 2024-07-24 2024-08-27 西北农林科技大学深圳研究院 除草剂抗性基因及其应用

Family Cites Families (200)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4693977A (en) 1982-08-23 1987-09-15 Queen's University At Kingston Enzyme immobilization for producing cephalosporin antibiotics
US4536475A (en) 1982-10-05 1985-08-20 Phytogen Plant vector
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NZ207765A (en) 1983-04-15 1987-03-06 Lubrizol Genetics Inc Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US6114138A (en) 1984-06-04 2000-09-05 Mycogen Plant Science, Inc. Insecticidal protein fragments
DE3587548T2 (de) 1984-12-28 1993-12-23 Bayer Ag Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann.
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US5420034A (en) 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US4886937A (en) 1985-05-20 1989-12-12 North Carolina State University Method for transforming pine
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
NZ217113A (en) 1985-08-07 1988-06-30 Monsanto Co Production of eucaryotic plants which are glyphosate resistant, vectors (transformation and expression), chimeric gene and plant cells
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
EP0242236B2 (en) 1986-03-11 1996-08-21 Plant Genetic Systems N.V. Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
US5013659A (en) 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
ATE87032T1 (de) 1986-12-05 1993-04-15 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5322938A (en) 1987-01-13 1994-06-21 Monsanto Company DNA sequence for enhancing the efficiency of transcription
US5359142A (en) 1987-01-13 1994-10-25 Monsanto Company Method for enhanced expression of a protein
TR27832A (tr) 1987-04-29 1995-08-31 Monsanto Co Zararli ucucu hasarata mukavim bitkiler.
US4971908A (en) 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
EP0342926B1 (en) 1988-05-17 1994-09-28 Mycogen Plant Science, Inc. Plant ubiquitin promoter system
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
DE3834738A1 (de) 1988-10-12 1990-04-19 Basf Lacke & Farben Verfahren zur herstellung eines mehrschichtigen ueberzuges, waessrige beschichtungszusammensetzungen, wasserverduennbare polyacrylatharze und verfahren zur herstellung von wasserverduennbaren polyacrylatharzen
US20020007053A1 (en) * 1989-02-06 2002-01-17 Barry Gerard Francis Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
EP0463056A1 (en) 1989-03-17 1992-01-02 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5126133A (en) 1989-06-27 1992-06-30 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5188960A (en) 1989-06-27 1993-02-23 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5501967A (en) 1989-07-26 1996-03-26 Mogen International, N.V./Rijksuniversiteit Te Leiden Process for the site-directed integration of DNA into the genome of plants
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6051753A (en) 1989-09-07 2000-04-18 Calgene, Inc. Figwort mosaic virus promoter and uses
GB9017539D0 (en) 1990-08-10 1990-09-26 Rhone Poulenc Agriculture New compositions of matter
GB8920519D0 (en) 1989-09-11 1989-10-25 Rhone Poulenc Ltd New compositions of matter
DE69033628T2 (de) 1989-10-31 2001-04-19 Monsanto Co., St. Louis Promotor für transgene Pflanzen
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
CA2074355C (en) 1990-01-22 2008-10-28 Ronald C. Lundquist Method of producing fertile transgenic corn plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
EP0546090B2 (en) * 1990-08-31 2006-07-19 Monsanto Company Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
JP2859427B2 (ja) 1990-11-21 1999-02-17 株式会社東芝 超電導コイル装置
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
GB9101659D0 (en) 1991-01-25 1991-03-06 Rhone Poulenc Agriculture Compositions of matter
GB9310203D0 (en) 1993-05-18 1993-06-30 Rhone Poulenc Agriculture Compositions of new matter
GB9101660D0 (en) 1991-01-25 1991-03-06 Rhone Poulenc Agriculture New compositions of matter
FR2673643B1 (fr) * 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
USRE36449E (en) 1991-03-05 1999-12-14 Rhone-Poulenc Agro Chimeric gene for the transformation of plants
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
GB9115377D0 (en) 1991-07-17 1991-09-04 Rhone Poulenc Agriculture New compositions of matter
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
GB9116834D0 (en) 1991-08-05 1991-09-18 Rhone Poulenc Agriculture Compositions of new matter
GB9304200D0 (en) 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
US5304730A (en) 1991-09-03 1994-04-19 Monsanto Company Virus resistant plants and method therefore
US5334753A (en) 1992-03-12 1994-08-02 Rhone-Poulenc Agriculture Ltd Processes for preparing ortho-substituted benzoic acids
US5510417A (en) 1992-04-21 1996-04-23 Toyo Boseki Kabushiki Kaisha Aqueous polyester dispersion suitable for use as a coating composition
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5332808A (en) 1992-09-08 1994-07-26 North Carolina State University DNA encoding a ribosome inactivating protein
US5607914A (en) 1993-01-13 1997-03-04 Pioneer Hi-Bred International, Inc. Synthetic antimicrobial peptides
GB9302071D0 (en) 1993-02-03 1993-03-24 Rhone Poulenc Agriculture Compositions of matter
DE69422837T2 (de) 1993-05-18 2000-08-17 Rhone-Poulenc Agriculture Ltd., Ongar 2-Cyan-1-sulfonamidophenyl-1,3-dion-Derivate und deren Verwendung als Herbizide
DE4317845A1 (de) 1993-05-28 1994-12-01 Bayer Ag Desoxyribonukleinsäuren
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5767373A (en) 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US5506195A (en) 1994-11-01 1996-04-09 Zeneca Limited Selective 1,3-cyclohexanedione corn herbicide
CN1134542C (zh) 1994-12-30 2004-01-14 赛迷尼思蔬菜种子公司 表达含有多个基因的dna构建体具有抗病毒抗性的转基因植物
GB9515941D0 (en) * 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
FR2734842B1 (fr) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides
ES2275278T3 (es) 1995-09-22 2007-06-01 Syngenta Limited Promotores de glutacion-s-transferasa de plantas.
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
IE80657B1 (en) 1996-03-29 1998-11-04 Merial Sas Insecticidal combination to control mammal fleas in particular fleas on cats and dogs
WO1997049816A1 (en) 1996-06-27 1997-12-31 E.I. Du Pont De Nemours And Company Plant gene for p-hydroxyphenylpyruvate dioxygenase
FR2751347B1 (fr) * 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides
US5850019A (en) 1996-08-06 1998-12-15 University Of Kentucky Research Foundation Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
US5994123A (en) 1996-08-09 1999-11-30 Regents Of University Of Minnesota Sugarcane bacilliform virus promoter
US6489462B1 (en) 1996-08-09 2002-12-03 Regents Of The University Of Minnesota Sugarcane bacilliform virus promoter
US6140078A (en) 1996-09-05 2000-10-31 Unilever Patent Holdings Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
US6096947A (en) 1997-01-14 2000-08-01 Pioneer Hi-Bred International, Inc. Methods for improving transformation efficiency
US6252138B1 (en) 1997-01-20 2001-06-26 Plant Genetic Systems, N.V. Pathogen-induced plant promoters
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US5922564A (en) 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
US5886775A (en) 1997-03-12 1999-03-23 M+Ind Noncontact digitizing imaging system
US7105724B2 (en) 1997-04-04 2006-09-12 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
GB9711015D0 (en) 1997-05-28 1997-07-23 Zeneca Ltd Improvements in or relating to organic compounds
WO1998056921A1 (en) 1997-06-12 1998-12-17 Dow Agrosciences Llc Regulatory sequences for transgenic plants
US6245968B1 (en) 1997-11-07 2001-06-12 Aventis Cropscience S.A. Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides
US6720475B1 (en) 1997-11-18 2004-04-13 Pioneer Hi-Bred International, Inc. Modified nucleic acid sequence encoding FLP recombinase
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
BR9907997A (pt) 1998-02-20 2000-10-24 Zeneca Ltd Promotor especìfico de pólen
DE69932868T2 (de) 1998-02-26 2007-03-15 Pioneer Hi-Bred International, Inc. Nukleinsäuremolekül mit einer Nukleotidsequenz für einen Promotor
EP1056862A1 (en) 1998-02-26 2000-12-06 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
US6037522A (en) * 1998-06-23 2000-03-14 Rhone-Poulenc Agro Agrobacterium-mediated transformation of monocots
JP2000083680A (ja) 1998-07-16 2000-03-28 Nippon Paper Industries Co Ltd 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ―
HUP0103993A3 (en) 1998-09-10 2003-10-28 Pioneer Hi Bred Internat Inc D Ecdysone receptors and methods for their use
WO2000024904A1 (en) 1998-10-23 2000-05-04 Mycogen Corporation PLANT-OPTIMIZED POLYNUCLEOTIDES ENCODING APPROXIMATELY 15 kDa AND APPROXIMATELY 45 kDa PESTICIDAL PROTEINS
US6489542B1 (en) * 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
US6515206B1 (en) 1998-12-23 2003-02-04 Calgene Llc Plastid transformation of Brassica
BRPI0007815B1 (pt) 1999-01-14 2016-04-19 Monsanto Technology Llc processo de transformação da soja
AU3210100A (en) 1999-01-19 2000-08-01 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
AU4133100A (en) 1999-04-29 2000-11-17 Syngenta Limited Herbicide resistant plants
PL357523A1 (en) 1999-04-29 2004-07-26 Syngenta Ltd. Herbicide resistant plants
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6677503B1 (en) 1999-06-23 2004-01-13 Pioneer Hi-Bred International, Inc. Sunflower anti-pathogene proteins and genes and their uses
AR025996A1 (es) 1999-10-07 2002-12-26 Valigen Us Inc Plantas no transgenicas resistentes a los herbicidas.
MXPA02007130A (es) 2000-01-21 2002-12-13 Pioneer Hi Bred Int Elementos promotores novedosos preferidos de raiz y metodos de uso..
US6388170B1 (en) 2000-04-07 2002-05-14 University Of Kentucky Research Foundation Bidirectional promoters and methods related thereto
US7169970B2 (en) * 2000-09-29 2007-01-30 Syngenta Limited Herbicide resistant plants
US7074985B2 (en) 2002-02-15 2006-07-11 Pioneer Hi-Bred International, Inc. Development of a stress-responsive promoter from maize
EP1504092B2 (en) 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
DE10224889A1 (de) 2002-06-04 2003-12-18 Metanomics Gmbh & Co Kgaa Verfahren zur stabilen Expression von Nukleinsäuren in transgenen Pflanzen
WO2004003177A2 (en) 2002-06-27 2004-01-08 Dow Agrosciences Llc Use of regulatory sequences in transgenic plants
AU2003247962B2 (en) * 2002-07-18 2008-06-12 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
ES2254043T3 (es) 2002-11-12 2011-10-24 ZAKRYTOE AKTSIONERNOE OBSCHESTVO " EVROGEN" Proteinas fluorescentes y cromoproteínas de especies de hidrozoos que no son aequorea y métodos para su uso.
DE60333486D1 (de) 2002-12-26 2010-09-02 Zakrytoe Aktsionernoe Obschest Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
EP1594961B1 (en) 2003-02-18 2013-12-25 Monsanto Technology LLC Glyphosate resistant class i 5-enolpyruvylshikimate-3-phosphate synthase (epsps)
WO2005014791A2 (en) 2003-08-08 2005-02-17 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
US20050102713A1 (en) 2003-11-07 2005-05-12 Merlo Donald J. Use of untranslated region of osmotin gene to enhance transgene expression in plants
US7317146B2 (en) 2003-12-31 2008-01-08 Pioneer Hi-Bred International, Inc. Production of cereal grain with reduced starch granule size and uses thereof
PL2308977T5 (pl) * 2004-04-30 2017-10-31 Dow Agrosciences Llc Nowy gen odporności na herbicydy
EP1753866B1 (en) * 2004-06-09 2010-09-22 Pioneer-Hi-Bred International, Inc. Plastid transit peptides
CN1973035A (zh) 2004-06-24 2007-05-30 孟山都技术有限公司 微生物草甘膦抗性5－烯醇丙酮基莽草酸－3－磷酸合酶
US8071305B2 (en) * 2004-08-13 2011-12-06 Athlomics Pty Ltd. Microarray-mediated diagnosis of herpes virus infection by monitoring host's differential gene expression upon infection
US7323556B2 (en) 2004-09-29 2008-01-29 Pioneer Hi-Bred International, Inc. Corn event DAS-59122-7 and methods for detection thereof
CN1789412B (zh) * 2004-12-16 2011-04-20 北京大学 利用片段互补技术重建5-烯醇丙酮酰莽草酸-3-磷酸合成酶活性
US20100029485A1 (en) * 2005-03-02 2010-02-04 Instituto Nacional De Tecnologia Agropecuaria Herbicide-resistant rice plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use
US20060207721A1 (en) 2005-03-17 2006-09-21 Greg Slominski Polymer adhesive splicing of water-soluble, orally ingestible thin film webs
NZ563000A (en) 2005-04-08 2009-10-30 Athenix Corp Identification of a new class of EPSP synthases
JP4772451B2 (ja) 2005-10-18 2011-09-14 株式会社リコー 画像形成装置
US8283522B2 (en) * 2005-10-28 2012-10-09 Dow Agrosciences, Llc. Herbicide resistance genes
UA92622C2 (ru) * 2005-12-01 2010-11-25 Атеникс Корпорейшн Гены grg23 та grg51, которые придают стойкость к гербицидам
AR057205A1 (es) * 2005-12-01 2007-11-21 Athenix Corp Genes grg23 y grg51 que confieren resistencia a herbicidas
US20080313769A9 (en) 2006-01-12 2008-12-18 Athenix Corporation EPSP synthase domains conferring glyphosate resistance
SI2465341T1 (sl) * 2006-01-12 2015-04-30 Cibus Europe B.V. Mutantni epsps
EP1984390A2 (en) 2006-01-23 2008-10-29 Board of Trustees of Michigan State University Methods for breeding glyphosate resistant plants and compositions thereof
US9045765B2 (en) 2006-06-09 2015-06-02 Athenix Corporation EPSP synthase genes conferring herbicide resistance
ATE491786T1 (de) 2006-06-09 2011-01-15 Athenix Corp Neuartige epsp-synthase-gene mit herbizidresistenz
USPP19043P3 (en) 2006-06-12 2008-07-22 Regents Of The University Of Minnesota Chrysanthemum plant named ‘95-169-8’
AU2007257704A1 (en) 2006-06-13 2007-12-21 Athenix Corporation Improved EPSP synthases: compositions and methods of use
CN101501197A (zh) * 2006-06-13 2009-08-05 阿则耐克斯公司 改善的epsp合酶：组合物及使用方法
AR061662A1 (es) * 2006-06-27 2008-09-10 Athenix Corp Grg33, grg35, grg36, grg37, grg38, grg39 y grg50: genes de epsp sintetasa que confieren resistencia a herbicidas
WO2008002962A2 (en) 2006-06-27 2008-01-03 Athenix Corporation Grg32: a novel epsp synthase gene conferring herbicide resistance
CN105296527B (zh) 2006-08-11 2020-11-27 陶氏益农公司 锌指核酸酶介导的同源重组
US8138393B2 (en) * 2006-08-11 2012-03-20 Monsanto Technology Llc Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase
CN101144087A (zh) 2006-09-15 2008-03-19 四川大学 盐生杜氏藻epsp合成酶及其制备方法与应用
UA111816C2 (uk) * 2006-10-25 2016-06-24 Монсанто Текнолоджи Ллс Спосіб обробки сільськогосподарських культур для боротьби з бур'янами
CA2671203C (en) 2006-11-29 2015-02-10 Athenix Corp. Improved grg23 epsp synthases: compositions and methods of use
BRPI0720226B8 (pt) 2006-12-07 2022-06-28 Dow Agrosciences Llc "vetor e métodos de seleção para uma célula de planta compreendendo um polinucleotídeo que codifica uma proteína que tem atividade de fosfinotricina acetil transferase, de uso de um gene de resistência a fosfinotricina como um marcador de resistência em células de planta, para geração de células de planta, plantas, e seus propagados resistentes à fosfinotricina, e de controle de pelo menos uma erva daninha em um campo".
ES2586210T3 (es) 2006-12-14 2016-10-13 Sangamo Biosciences, Inc. Proteínas de dedo de zinc no canónicas optimizadas
US7838729B2 (en) * 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
US8609936B2 (en) * 2007-04-27 2013-12-17 Monsanto Technology Llc Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis
EP2145007B1 (en) * 2007-05-09 2017-01-25 Dow AgroSciences LLC Novel herbicide resistance genes
NZ584406A (en) 2007-10-05 2013-07-26 Dow Agrosciences Llc Methods for transferring molecular substances into plant cells using nanoparticles
AU2008321220A1 (en) * 2007-11-15 2009-05-22 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event MON87701 and methods for detection thereof
CN102046796B (zh) * 2008-02-10 2014-12-10 孟山都技术公司 用于植物害虫控制的方法和组合物
US9629366B2 (en) * 2008-05-21 2017-04-25 Basf Se Herbicidal composition comprising glyphosate, glufosinate or their salts
EP2299804A4 (en) * 2008-06-11 2011-05-18 Dow Agrosciences Llc RECOMBINANT PRODUCTS FOR EXPRESSION OF HERBICIDE TOLERANT GENES, ASSOCIATED PLANTS, AND COMBINATIONS OF ASSOCIATED CHARACTERS
WO2010012796A1 (en) 2008-08-01 2010-02-04 Bayer Bioscience N.V. A method for increasing photosynthetic carbon fixation in rice
EP2334795B1 (en) 2008-09-08 2014-04-30 Athenix Corporation Compositions and methods for expression of a heterologous nucleotide sequence in plants
US20100107446A1 (en) 2008-11-05 2010-05-06 Doris Peet Shoe accessory
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
BRPI0922641B1 (pt) * 2008-12-17 2021-10-26 Dow Agrosciences Llc Método de integração de uma ou mais sequências de ácido nucleico exógenas no genoma de uma célula vegetal
AU2009333021B2 (en) * 2008-12-31 2015-09-10 Sapphire Energy, Inc. Genetically engineered herbicide resistant algae
CN101886088B (zh) * 2009-05-15 2013-04-17 北京大学 转基因构建体和转基因植物
CN102471778A (zh) * 2009-07-21 2012-05-23 先正达参股股份有限公司 源自低等光合真核生物的质体转运肽及其方法
US8598413B2 (en) 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
US8578771B2 (en) 2010-03-05 2013-11-12 Lockheed Martin Corporation Compact gradiometer with accelerometer based rotational control
CN101831443A (zh) 2010-03-25 2010-09-15 中国农业科学院生物技术研究所 编码5-烯醇式丙酮酰莽草酸-3-磷酸合酶的基因及其应用
AU2011264879B2 (en) 2010-06-11 2015-11-12 Syngenta Crop Protection Ag Compositions and methods for protein production
US9139842B2 (en) 2010-08-13 2015-09-22 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
US9150625B2 (en) * 2011-05-23 2015-10-06 E I Du Pont De Nemours And Company Chloroplast transit peptides and methods of their use
BR102013002599B1 (pt) * 2012-02-01 2022-04-05 Dow Agrosciences Llc Molécula de ácido nucleico isolada ou recombinante, método para gerar uma célula vegetal tolerante a glifosato e usos da mesma
AU2013205557B2 (en) * 2012-04-17 2016-04-21 Corteva Agriscience Llc Synthetic brassica-derived chloroplast transit peptides
KR20180038048A (ko) * 2015-08-20 2018-04-13 다우 아그로사이언시즈 엘엘씨 엽록체 전이 펩티드

Also Published As

Publication number Publication date
AU2015210439A1 (en) 2015-09-03
RU2626535C2 (ru) 2017-07-28
AU2015210439B2 (en) 2017-05-25
AR089873A1 (es) 2014-09-24
UA116198C2 (uk) 2018-02-26
KR20140119186A (ko) 2014-10-08
RU2017132988A3 (ru) 2019-02-07
MX348268B (es) 2017-06-05
BR112014018916B1 (pt) 2022-08-09
WO2013116758A1 (en) 2013-08-08
BR102013002598A2 (pt) 2019-03-06
AU2013205441A1 (en) 2013-08-15
KR20140119187A (ko) 2014-10-08
RU2014135340A (ru) 2016-03-20
UA115546C2 (uk) 2017-11-27
EP2809143A1 (en) 2014-12-10
US9382549B2 (en) 2016-07-05
AU2016202277B2 (en) 2017-10-05
RU2014135335A (ru) 2016-03-20
US10428339B2 (en) 2019-10-01
AU2016202277A1 (en) 2016-05-05
MX343876B (es) 2016-11-25
CA2863194A1 (en) 2013-08-08
PH12014501728A1 (en) 2014-11-17
BR102013002592B1 (pt) 2022-06-07
BR112014018832A2 (pt) 2017-07-04
US9359614B2 (en) 2016-06-07
US20130217577A1 (en) 2013-08-22
MX349666B (es) 2017-08-08
JP2015506701A (ja) 2015-03-05
RU2014135403A (ru) 2016-03-27
AU2013205606A1 (en) 2013-08-15
MX351310B (es) 2017-10-09
MX2014009342A (es) 2015-02-20
CL2014002021A1 (es) 2014-11-21
BR112014018903A2 (pt) 2020-10-27
CN107119060A (zh) 2017-09-01
CN104202967A (zh) 2014-12-10
US20170114332A1 (en) 2017-04-27
JP2015506703A (ja) 2015-03-05
JP2015505480A (ja) 2015-02-23
UY34606A (es) 2013-09-02
ZA201406143B (en) 2016-06-29
US20130219552A1 (en) 2013-08-22
WO2013116768A1 (en) 2013-08-08
EP2809148A1 (en) 2014-12-10
EP3219200A1 (en) 2017-09-20
JP6466172B2 (ja) 2019-02-06
EP2809142A1 (en) 2014-12-10
CN104219949A (zh) 2014-12-17
PH12014501726B1 (en) 2019-11-29
AU2013205604B2 (en) 2015-05-07
US9493780B2 (en) 2016-11-15
CA2863194C (en) 2021-07-27
WO2013116700A1 (en) 2013-08-08
RU2014135442A (ru) 2016-03-27
NZ628549A (en) 2016-10-28
TWI637054B (zh) 2018-10-01
AR090416A1 (es) 2014-11-12
CA2863402A1 (en) 2013-08-08
NZ712168A (en) 2016-05-27
US9506077B2 (en) 2016-11-29
US9540654B2 (en) 2017-01-10
RU2017133853A (ru) 2019-02-07
KR102036395B1 (ko) 2019-10-24
CN104219950B (zh) 2018-02-06
AU2013205441B2 (en) 2016-10-27
CO7061038A2 (es) 2014-09-19
TW201336991A (zh) 2013-09-16
AU2015215946A1 (en) 2015-09-17
BR112014019043B1 (pt) 2022-08-09
KR20140132354A (ko) 2014-11-17
AU2017200562A1 (en) 2017-02-23
BR102013002599A2 (pt) 2015-08-11
RU2017133853A3 (ru) 2019-02-07
CA2863196C (en) 2021-08-10
MX378692B (es) 2025-03-10
AP2014007885A0 (en) 2014-08-31
EP3219200B1 (en) 2020-05-06
EP2809143A4 (en) 2015-12-16
MX354522B (es) 2018-03-08
JP6242345B2 (ja) 2017-12-06
EP2809145A1 (en) 2014-12-10
KR102078764B1 (ko) 2020-02-19
EP2809148B1 (en) 2019-05-15
MX344968B (es) 2017-01-12
CN104219950A (zh) 2014-12-17
US20170073696A1 (en) 2017-03-16
CA2863400A1 (en) 2013-08-08
BR112014018916A2 (pt) 2017-07-04
CN107119061A (zh) 2017-09-01
AR090418A1 (es) 2014-11-12
BR112014018916B8 (pt) 2023-05-16
US20130295638A1 (en) 2013-11-07
RU2721122C2 (ru) 2020-05-18
RU2014135466A (ru) 2016-03-20
RU2636037C2 (ru) 2017-11-17
KR20140132352A (ko) 2014-11-17
NZ629394A (en) 2015-09-25
US20130205440A1 (en) 2013-08-08
AR090417A1 (es) 2014-11-12
AP2014007895A0 (en) 2014-08-31
WO2013116764A1 (en) 2013-08-08
CN108192901A (zh) 2018-06-22
CL2014002017A1 (es) 2014-11-21
MX2014009349A (es) 2016-06-21
JP2015506702A (ja) 2015-03-05
US20160304897A1 (en) 2016-10-20
AR089874A1 (es) 2014-09-24
US20130212737A1 (en) 2013-08-15
MX345618B (es) 2017-02-07
US10577619B2 (en) 2020-03-03
CN104219949B (zh) 2018-03-30
EP3235373B1 (en) 2020-05-13
US20130205441A1 (en) 2013-08-08
PH12014501729A1 (en) 2014-11-10
BR102013002592A2 (pt) 2015-08-04
US9873883B2 (en) 2018-01-23
BR102013002599B1 (pt) 2022-04-05
EP2809147A4 (en) 2015-08-05
AU2013205525A1 (en) 2013-08-15
AR091306A1 (es) 2015-01-28
MX2014009345A (es) 2015-10-05
AU2013205521B2 (en) 2016-10-13
ZA201405891B (en) 2016-08-31
CA2863196A1 (en) 2013-08-08
IL233861B (en) 2018-04-30
CL2014002018A1 (es) 2014-11-21
US20170022517A1 (en) 2017-01-26
CA2863405C (en) 2023-09-19
TW201335365A (zh) 2013-09-01
EP3470522A2 (en) 2019-04-17
EP2809145A4 (en) 2015-09-16
RU2014135324A (ru) 2016-03-20
MX2014009348A (es) 2015-02-20
ZA201406003B (en) 2015-11-25
UA115547C2 (uk) 2017-11-27
AU2015215946B2 (en) 2017-06-22
AU2013205600A1 (en) 2013-08-15
AU2013205521A1 (en) 2013-08-15
BR112014019043B8 (pt) 2023-05-16
PH12014501726A1 (en) 2014-11-10
CN110577933A (zh) 2019-12-17
JP2019047803A (ja) 2019-03-28
CN108285901A (zh) 2018-07-17
EP3235373A1 (en) 2017-10-25
IL233911B (en) 2018-08-30
TW201341394A (zh) 2013-10-16
BR112014019043A2 (pt) 2017-07-04
BR102013002598B1 (pt) 2022-05-31
KR102047336B1 (ko) 2019-11-22
CN104202968A (zh) 2014-12-10
US20130210114A1 (en) 2013-08-15
US10577618B2 (en) 2020-03-03
CL2014002016A1 (es) 2014-11-21
US9493781B2 (en) 2016-11-15
HUE042672T2 (hu) 2019-07-29
JP6251193B2 (ja) 2017-12-20
KR20140120934A (ko) 2014-10-14
MX2014009351A (es) 2015-02-20
JP2015511815A (ja) 2015-04-23
CN104519734A (zh) 2015-04-15
CA2863405A1 (en) 2013-08-08
CO7061037A2 (es) 2014-09-19
AU2013205604A1 (en) 2013-08-15
US20170058286A1 (en) 2017-03-02
RU2636036C2 (ru) 2017-11-17
KR102045097B1 (ko) 2019-11-14
ZA201406142B (en) 2016-06-29
AU2013205600B2 (en) 2016-05-19
UA116980C2 (uk) 2018-06-11
EP3470522A3 (en) 2019-06-19
AU2018253607A1 (en) 2018-11-22
CA2863201A1 (en) 2013-08-08
EP2809142A4 (en) 2015-09-16
UY34608A (es) 2013-09-02
NZ628989A (en) 2016-07-29
RU2720973C2 (ru) 2020-05-15
AU2017200562B2 (en) 2018-07-26
UA115545C2 (uk) 2017-11-27
EP2809144B1 (en) 2019-11-13
EP2809147A1 (en) 2014-12-10
IL233860B (en) 2019-01-31
US10421972B2 (en) 2019-09-24
AP2014007887A0 (en) 2014-08-31
KR20140132353A (ko) 2014-11-17
WO2013116773A1 (en) 2013-08-08
ZA201406338B (en) 2016-05-25
EP2809144A4 (en) 2015-10-21
CL2014002015A1 (es) 2014-11-21
CO7061041A2 (es) 2014-09-19
WO2013116782A1 (en) 2013-08-08
IL233858A (en) 2017-10-31
PH12014501727A1 (en) 2014-11-10
CO7051012A2 (es) 2014-09-10
ZA201406002B (en) 2016-08-31
CO7061036A2 (es) 2014-09-19
NZ629051A (en) 2016-10-28
CO7061035A2 (es) 2014-09-19
RU2627169C2 (ru) 2017-08-03
JP2015511126A (ja) 2015-04-16
ES2716481T3 (es) 2019-06-12
EP2809144A1 (en) 2014-12-10
JP6349261B2 (ja) 2018-06-27
RU2634411C2 (ru) 2017-10-26
JP2018068304A (ja) 2018-05-10
JP6537825B2 (ja) 2019-07-10
AU2018253607B2 (en) 2021-05-06
MX2014009343A (es) 2015-06-05
AU2013205606B2 (en) 2015-05-21
IL243904A0 (en) 2016-04-21
CA2863400C (en) 2022-06-14
EP2809148A4 (en) 2015-12-09
US20200157555A1 (en) 2020-05-21
RU2632648C2 (ru) 2017-10-06
EP2809147B1 (en) 2018-09-19
CL2014002020A1 (es) 2014-11-21
EP2809142B1 (en) 2019-05-15
MX352662B (es) 2017-12-04
BR122022004161B1 (pt) 2022-11-08
UY34607A (es) 2013-09-02
AR089872A1 (es) 2014-09-24
CN104202969A (zh) 2014-12-10
AU2013205525B2 (en) 2016-12-22
AP2014007886A0 (en) 2014-08-31

Similar Documents

Publication Publication Date Title
RU2017132988A (ru) Синтетические транзитные пептиды хлоропластов
MX2014000993A (es) Gen de la toxina axmi270 y sus metodos de uso.
MX357562B (es) Axmi-115, axmi-113, axmi-005, axmi-163 y axmi-184: proteinas insecticidas vip3a de bacillus thuringiensis y metodos para su uso.
WO2011103247A3 (en) Axmi218, axmi219, axmi220, axmi226, axmi227, axmi228, axmi229, axmi230, and axmi231 delta-endotoxin genes and methods for their use
MX2011008955A (es) Proteinas pesticidas y metodos de uso de las mismas.
MX2014001070A (es) Gen plaguicida axmi279 y sus metodos de uso.
MX351526B (es) Proteinas variantes axmi205 y sus metodos de uso.
MX362999B (es) Gen de la delta-endotoxina axmi345 y sus metodos de uso.
HRP20221089T1 (hr) Optimizirani geni faktora viii
HRP20182063T4 (hr) Gen otpornosti na rizomaniju
MX2012000202A (es) Gen plaguicida axmi-205 y metodos para su uso.
MX347768B (es) Genes delta-endotoxinicos axmi221z, axmi222z, axmi223z, axmi224z, y axmi225z y metodos para su uso.
MX354219B (es) Familia de genes plaguicidas axmi-192 y metodos para su uso.
WO2013186563A3 (en) Factor viii sequences
RU2011147121A (ru) Промотор целого семени
WO2009099906A3 (en) Directed evolution of grg31 and grg36 epsp synthase enzymes
GB201201178D0 (en) Novel enzymes
MX2019013438A (es) Genes de toxina y metodos para su uso.
EA201270107A1 (ru) Способы и средства для получения растений с повышенной устойчивостью к глифосату
WO2012135501A3 (en) Axmi232, axmi233, and axmi249 toxin genes and methods for their use
CA2735646A1 (en) Transgenic plants with enhanced growth characteristics
JP2010207199A5 (ru)
CN102387701A (zh) 植物谷氨酰胺苯丙酮酸转氨酶基因以及携带该基因的转基因植物
MY156447A (en) Methods and polynucleotides for improving plants
WO2007146765A3 (en) Novel epsp synthase genes conferring herbicide resistance

Legal Events

Date Code Title Description
PD4A Correction of name of patent owner