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AR057205A1 - Genes grg23 y grg51 que confieren resistencia a herbicidas - Google Patents

Genes grg23 y grg51 que confieren resistencia a herbicidas

Info

Publication number
AR057205A1
AR057205A1 ARP060105284A ARP060105284A AR057205A1 AR 057205 A1 AR057205 A1 AR 057205A1 AR P060105284 A ARP060105284 A AR P060105284A AR P060105284 A ARP060105284 A AR P060105284A AR 057205 A1 AR057205 A1 AR 057205A1
Authority
AR
Argentina
Prior art keywords
seq
nucleotide sequence
amino acid
nucleic acid
acid molecule
Prior art date
Application number
ARP060105284A
Other languages
English (en)
Original Assignee
Athenix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Athenix Corp filed Critical Athenix Corp
Publication of AR057205A1 publication Critical patent/AR057205A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Composiciones y métodos que confieren resistencia o tolerancia a herbicidas a bacterias, plantas, células vegetales, tejidos vegetales y semillas. Las composiciones incluyen polinucleotidos que codifican polipéptidos de resistencia o tolerancia a herbicidas, vectores que comprenden dichos polinucleotidos y células huésped que comprenden a dichos vectores. Las secuencias de nucleotidos de la presente se pueden usar en construcciones de ADN o casetes de expresion para la transformacion y expresion en organismos, incluyendo microorganismos y plantas. Las composiciones también comprenden bacterias, plantas, células vegetales, tejidos vegetales y semillas transformadas. En particular, se proveen polinucleotidos aislados que codifican polipéptidos de resistencia o tolerancia a glifosato. Además. se incluyen las secuencias de aminoácidos correspondientes a los polinucleotidos. En particular, la presente provee polinucleotidos aislados que comprenden secuencias de nucleotidos que codifican la secuencia de aminoácidos de la SEQ ID N°: 2, 4 o 6, o la secuencia de nucleotidos que se muestra en la SEQ ID N°: 1, 3 o 5. La presente provee además un método para medir la cinética de la actividad enzimática usando sustratos fluorogénicos. Reivindicacion 1: Una molécula aislada de ácido nucleico, caracterizada porque se selecciona del grupo formado por: a) una molécula de ácido nucleico que comprende la secuencia de nucleotidos de la SEQ ID N°: 1, 3 o 5, o un complemento de la misma; b) una molécula de ácido nucleico que comprende una secuencia de nucleotidos que presenta al menos un 80% de identidad de secuencia con la secuencia de nucleotidos de la SEQ ID N°: 1, 3 o 5, o un complemento de la misma; c) la secuencia de nucleotidos de resistencia a herbicidas del inserto de ADN del plásmido depositado con el N° de Acceso NRRL B-30888 o NRRL B-30949, o un complemento de la misma; d) una molécula de ácido nucleico que codifica un polipéptido que comprende la secuencia de aminoácidos de la SEQ ID N°: 2, 4 o 6; y e) una molécula de ácido nucleico que comprende una secuencia de nucleotidos que codifica un polipéptido que presenta al menos un 80% de identidad de secuencia de aminoácidos con la secuencia de aminoácidos de la SEQ ID N°: 2, 4 o 6.
ARP060105284A 2005-12-01 2006-11-29 Genes grg23 y grg51 que confieren resistencia a herbicidas AR057205A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74116605P 2005-12-01 2005-12-01
US81779906P 2006-06-30 2006-06-30

Publications (1)

Publication Number Publication Date
AR057205A1 true AR057205A1 (es) 2007-11-21

Family

ID=37814650

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP060105284A AR057205A1 (es) 2005-12-01 2006-11-29 Genes grg23 y grg51 que confieren resistencia a herbicidas

Country Status (18)

Country Link
US (2) US7674958B2 (es)
EP (2) EP1963510B1 (es)
AR (1) AR057205A1 (es)
AT (1) ATE440954T1 (es)
AU (1) AU2006320516C1 (es)
BR (1) BRPI0619092A2 (es)
CA (1) CA2631406C (es)
DE (1) DE602006008827D1 (es)
DK (1) DK1963510T3 (es)
ES (1) ES2332380T3 (es)
HR (1) HRP20090610T1 (es)
NZ (1) NZ569433A (es)
PL (1) PL1963510T3 (es)
PT (1) PT1963510E (es)
RS (1) RS51134B (es)
RU (1) RU2393225C2 (es)
SI (1) SI1963510T1 (es)
WO (1) WO2007064828A2 (es)

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US7989679B2 (en) * 2006-03-02 2011-08-02 Athenix Corp. Methods and compositions for improved enzyme activity in transgenic plants
US9045765B2 (en) * 2006-06-09 2015-06-02 Athenix Corporation EPSP synthase genes conferring herbicide resistance
HRP20110587T1 (hr) * 2006-11-29 2011-09-30 Athenix Corporation Poboljšane grg23 epsp sintaze: pripravci i postupci uporabe
CN101429499B (zh) * 2007-11-09 2010-08-11 中国农业科学院生物技术研究所 高耐受草甘膦的epsp合酶及其编码序列
US7960616B2 (en) 2008-02-01 2011-06-14 Athenix Corp. Directed evolution of GRG31 EPSP synthase enzyme
EA201270107A1 (ru) 2009-07-01 2012-07-30 Байер Байосайенс Н.В. Способы и средства для получения растений с повышенной устойчивостью к глифосату
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EP2580336B1 (en) 2010-06-09 2017-05-10 Bayer CropScience NV Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
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Also Published As

Publication number Publication date
DE602006008827D1 (de) 2009-10-08
EP1963510B1 (en) 2009-08-26
PT1963510E (pt) 2009-11-30
EP2078754B1 (en) 2014-04-09
US20070136840A1 (en) 2007-06-14
EP2078754A2 (en) 2009-07-15
EP2078754A3 (en) 2010-01-20
US20090227771A1 (en) 2009-09-10
RS51134B (sr) 2010-10-31
ES2332380T3 (es) 2010-02-03
EP1963510A2 (en) 2008-09-03
HRP20090610T1 (hr) 2010-03-31
WO2007064828A2 (en) 2007-06-07
PL1963510T3 (pl) 2010-02-26
ATE440954T1 (de) 2009-09-15
AU2006320516B2 (en) 2013-03-21
US8309332B2 (en) 2012-11-13
CA2631406A1 (en) 2007-06-07
CA2631406C (en) 2014-10-14
DK1963510T3 (da) 2009-12-21
NZ569433A (en) 2011-11-25
RU2393225C2 (ru) 2010-06-27
AU2006320516A1 (en) 2007-06-07
AU2006320516C1 (en) 2013-08-15
RU2008126711A (ru) 2010-01-10
SI1963510T1 (sl) 2010-01-29
BRPI0619092A2 (pt) 2011-09-13
WO2007064828A3 (en) 2007-08-02
US7674958B2 (en) 2010-03-09

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