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AR127904A1 - Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas - Google Patents

Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas

Info

Publication number
AR127904A1
AR127904A1 ARP220103369A ARP220103369A AR127904A1 AR 127904 A1 AR127904 A1 AR 127904A1 AR P220103369 A ARP220103369 A AR P220103369A AR P220103369 A ARP220103369 A AR P220103369A AR 127904 A1 AR127904 A1 AR 127904A1
Authority
AR
Argentina
Prior art keywords
zip
plant
methods
transcription factor
fertility
Prior art date
Application number
ARP220103369A
Other languages
English (en)
Inventor
Marisa Miller
Devin Oconnor
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of AR127904A1 publication Critical patent/AR127904A1/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Esta invención se refiere a composiciones y métodos para modificar factores de transcripción de la cremallera de homeodominio-leucina (HD-Zip) que regulan la fertilidad de los floretes, el número de semillas y/o el peso de la semilla en las plantas. La invención se refiere además a plantas producidas usando los métodos y las composiciones de la invención. Reivindicación 1: Una planta o parte de ella que comprende al menos una mutación en un gen endógeno del factor de transcripción de cremallera de homeodominio-leucina (HD-Zip) que codifica un factor de transcripción de HD-Zip. Reivindicación 28: La planta o parte de ella de cualquiera de las reivindicaciones anteriores, en donde la planta o parte de ella que comprende la al menos una mutación exhibe un fenotipo de mayor fertilidad de los floretes, mayor número de semillas, mayor peso de las semillas, mayor tamaño de los órganos florales, mayor ancho de la mazorca y/o mayor longitud de la mazorca. Reivindicación 29: Una célula vegetal que comprende un sistema de edición, donde el sistema de edición comprende: (a) una proteína efectora asociada con CRISPR-Cas; y (b) un ácido nucleico guía (por ejemplo, gRNA, gDNA, crRNA, crDNA) que tiene una secuencia espaciadora con complementariedad con un gen blanco endógeno que codifica un factor de transcripción de HD-Zip. Reivindicación 127: El método de cualquiera de las reivindicaciones 120 - 126, en donde el polinucleótido de interés es un polinucleótido que confiere tolerancia a herbicidas, resistencia a insectos, resistencia a enfermedades, mayor rendimiento, mayor eficacia en el uso de nutrientes o resistencia al estrés abiótico.
ARP220103369A 2021-12-09 2022-12-07 Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas AR127904A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163287610P 2021-12-09 2021-12-09

Publications (1)

Publication Number Publication Date
AR127904A1 true AR127904A1 (es) 2024-03-06

Family

ID=85157366

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220103369A AR127904A1 (es) 2021-12-09 2022-12-07 Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas

Country Status (8)

Country Link
US (1) US20230183730A1 (es)
EP (1) EP4444743A1 (es)
CN (1) CN118613495A (es)
AR (1) AR127904A1 (es)
CA (1) CA3240821A1 (es)
MX (1) MX2024006590A (es)
UY (1) UY40060A (es)
WO (1) WO2023108035A1 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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