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AR105968A1 - Reducción del contenido de asparagina en plantas - Google Patents

Reducción del contenido de asparagina en plantas

Info

Publication number
AR105968A1
AR105968A1 ARP160102746A ARP160102746A AR105968A1 AR 105968 A1 AR105968 A1 AR 105968A1 AR P160102746 A ARP160102746 A AR P160102746A AR P160102746 A ARP160102746 A AR P160102746A AR 105968 A1 AR105968 A1 AR 105968A1
Authority
AR
Argentina
Prior art keywords
sec
plant
ident
asparagine
plant material
Prior art date
Application number
ARP160102746A
Other languages
English (en)
Inventor
Bovet Lucien
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of AR105968A1 publication Critical patent/AR105968A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05—Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05004—Asparagine synthase (glutamine-hydrolyzing) (6.3.5.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

En la presente descripción se describe una planta mutante, de origen no natural o transgénica o parte de esta que tiene expresión o actividad de asparagina sintetasa reducida, dicha asparagina sintetasa comprende, consiste o consiste esencialmente en: (i) una secuencia de polinucleótido que comprende, que consiste o que consiste esencialmente en una secuencia que tiene al menos 90% de identidad de secuencia con la sec. con núm. de ident.: 1 o al menos 72% de identidad de secuencia con la sec. con núm. de ident.: 3 o la sec. con núm. de ident.: 5 o la sec. con núm. de ident.: 7; o (ii) un polipéptido codificado por el polinucleótido que se expone en (i); o (iii) un polipéptido que tiene al menos 78% de identidad de secuencia con la sec. con núm. de ident.: 2 o la sec. con núm. de ident.: 4 o la sec. con núm. de ident.: 6 o la sec. con núm. de ident.: 8; en donde la expresión o la actividad de la asparagina sintetasa que se expone en (i), (ii) o (iii) se encuentra reducida en comparación con una planta control. Reivindicación 11: Un método para preparar material vegetal con niveles reducidos de asparagina y niveles reducidos de acrilamida en el aerosol derivado de dicho material vegetal en comparación con el material vegetal de una planta control, dicho método comprende las etapas de: (a) proporcionar una planta o parte de esta que comprende un polinucleótido que comprende, que consiste o que consiste esencialmente en una secuencia que codifica una asparagina sintetasa y que tiene al menos 90% de identidad de secuencia con la sec. con núm. de ident.: 1 o al menos 72% de identidad de secuencia con la sec. con núm. de ident.: 3 o la sec. con núm. de ident.: 5 o la sec. con núm. de ident.: 7; (b) reducir la expresión del polinucleótido o la actividad de la proteína codificada de esta manera en la planta o parte de esta; (c) cosechar material vegetal de la planta o parte de esta; (d) secar o curar el material vegetal; (e) opcionalmente, medir los niveles de asparagina en la planta o parte de esta y/o medir los niveles de acrilamida en el aerosol derivado de la planta o parte de esta; y (f) obtener material vegetal curado o seco con niveles reducidos de asparagina y niveles reducidos de acrilamida en el aerosol derivado de dicho material vegetal, de manera adecuada, en donde el nivel de nicotina es esencialmente el mismo que el nivel de nicotina en la planta control; de manera adecuada, en donde la formación de glutamina, ácido aspártico y ácido glutámico aumenta en comparación con la hoja curada o seca de la planta control.
ARP160102746A 2015-09-09 2016-09-08 Reducción del contenido de asparagina en plantas AR105968A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15184528 2015-09-09

Publications (1)

Publication Number Publication Date
AR105968A1 true AR105968A1 (es) 2017-11-29

Family

ID=54105683

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160102746A AR105968A1 (es) 2015-09-09 2016-09-08 Reducción del contenido de asparagina en plantas

Country Status (21)

Country Link
US (1) US10883114B2 (es)
EP (1) EP3346826B8 (es)
JP (1) JP6810134B2 (es)
KR (1) KR102207550B1 (es)
CN (1) CN107920489A (es)
AR (1) AR105968A1 (es)
AU (1) AU2016318423B2 (es)
BR (1) BR112018002914A2 (es)
CA (1) CA2998286C (es)
ES (1) ES3014919T3 (es)
HK (1) HK1255341A1 (es)
IL (1) IL257305B (es)
MX (1) MX392152B (es)
MY (1) MY187046A (es)
NZ (1) NZ738897A (es)
PH (1) PH12017502292A1 (es)
PL (1) PL3346826T3 (es)
RU (1) RU2742725C2 (es)
UA (1) UA125478C2 (es)
WO (1) WO2017042162A1 (es)
ZA (1) ZA201708411B (es)

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Publication number Priority date Publication date Assignee Title
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
KR102826087B1 (ko) 2018-03-28 2025-06-27 필립모리스 프로덕츠 에스.에이. 식물 내 환원당 함량 조절
CN113271768B (zh) 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
JP7659547B2 (ja) 2019-10-01 2025-04-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節（ｉｎｖ）
JP2022552638A (ja) * 2019-10-01 2022-12-19 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における糖およびアミノ酸含有量の調節（ｓｕｌｔｒ３）
CN112931182B (zh) * 2021-01-29 2022-06-07 四川农业大学 一种提高小麦染色体重组频率的方法
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114766359B (zh) * 2022-04-22 2023-03-31 华中农业大学 一种试管苗支架及其在柑橘茎尖微芽嫁接脱毒中的应用
WO2024079137A1 (en) 2022-10-13 2024-04-18 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2
CN120530195A (zh) 2023-02-02 2025-08-22 菲利普莫里斯生产公司 糖转运蛋白的调节
EP4658670A1 (en) 2023-02-02 2025-12-10 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
CN116574719B (zh) * 2023-06-28 2024-06-25 四川农业大学 AsMIPS1基因克隆、表达载体构建和应用
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN119876252B (zh) * 2025-03-27 2025-06-20 浙江农林大学 毛竹PeNRAMP5基因在降低水稻Cd积累增加Mn吸收的应用

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Also Published As

Publication number Publication date
BR112018002914A2 (pt) 2018-11-06
WO2017042162A1 (en) 2017-03-16
CA2998286A1 (en) 2017-03-16
ES3014919T3 (en) 2025-04-28
AU2016318423B2 (en) 2022-01-20
MX2018002695A (es) 2018-04-13
IL257305A (en) 2018-03-29
RU2742725C2 (ru) 2021-02-10
CN107920489A (zh) 2018-04-17
ZA201708411B (en) 2019-01-30
EP3346826C0 (en) 2025-02-19
KR20180051530A (ko) 2018-05-16
PL3346826T3 (pl) 2025-06-09
US20190169628A1 (en) 2019-06-06
KR102207550B1 (ko) 2021-01-27
RU2018112407A (ru) 2019-10-09
JP2018527934A (ja) 2018-09-27
EP3346826B8 (en) 2025-04-16
MY187046A (en) 2021-08-27
EP3346826B1 (en) 2025-02-19
JP6810134B2 (ja) 2021-01-06
CA2998286C (en) 2023-11-07
UA125478C2 (uk) 2022-03-30
HK1255341A1 (zh) 2019-08-16
PH12017502292A1 (en) 2018-06-25
IL257305B (en) 2022-06-01
US10883114B2 (en) 2021-01-05
MX392152B (es) 2025-03-21
AU2016318423A1 (en) 2018-04-05
NZ738897A (en) 2019-10-25
EP3346826A1 (en) 2018-07-18
RU2018112407A3 (es) 2020-02-25

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