AR041375A1 - Vector para la produccion de plantas angiospermas transplastomicas - Google Patents
Vector para la produccion de plantas angiospermas transplastomicasInfo
- Publication number
- AR041375A1 AR041375A1 ARP030103474A ARP030103474A AR041375A1 AR 041375 A1 AR041375 A1 AR 041375A1 AR P030103474 A ARP030103474 A AR P030103474A AR P030103474 A ARP030103474 A AR P030103474A AR 041375 A1 AR041375 A1 AR 041375A1
- Authority
- AR
- Argentina
- Prior art keywords
- plants
- genes
- vector
- gene
- transplastomic
- Prior art date
Links
- 239000013598 vector Substances 0.000 title abstract 7
- 241000218922 Magnoliophyta Species 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 10
- 241000196324 Embryophyta Species 0.000 abstract 6
- 108020004414 DNA Proteins 0.000 abstract 4
- 101100301006 Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) cbbL2 gene Proteins 0.000 abstract 3
- 101150026213 atpB gene Proteins 0.000 abstract 3
- 101150004101 cbbL gene Proteins 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 210000002706 plastid Anatomy 0.000 abstract 3
- 101150074945 rbcL gene Proteins 0.000 abstract 3
- 108091029795 Intergenic region Proteins 0.000 abstract 2
- 101150035600 atpD gene Proteins 0.000 abstract 2
- 101150038923 atpF gene Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 1
- 239000004062 cytokinin Substances 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 abstract 1
- 229960001669 kinetin Drugs 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Un vector de ADN que permite la inserción estable y la expresión de genes heterólogos en los genomas de plastidios de células de cualquier planta Angiosperma.. En este vector los genes a introducir se pueden insertar en múltiples sitios de clonaciónlocalizados en una región intergénica artificial que se obtiene de combinar los operones atpB y rbcL pertenecientes a plantas de clases diferentes (dicotiledóneas o monocotiledóneas). El casete de expresión se integra al genoma medianterecombinación entre las secuencias bordes atpB y rbcL del vector, con las correspondientes regiones homólogas del genoma plastídico; pudiendo expresarse más de un gen de interés a partir del promotor rbcL adyacente a la región borde que codificapara el gen atpB. Reivindicación 1: Un vector de ADN útil para la transformación estable y expresión de genes en plastidios, donde el gen a expresar se introduce en una región intergénica artificial formada por la combinación de dos regiones 5' notraducibles de genes que se transcriben en direcciones divergentes y pertenecientes a plantas de divisiones o clases diferentes. Reivindicación 116: El cultivo de las células vegetales en presencia de citoquininas, antes de introducirles cualquierade los vectores de ADN enunciados en las reivindicaciones de al 1 a la 115, para aumentar la frecuencia de producción de plantas transplastómicas. Reivindicación 117: El empleo de kinetina según la reivindicación 116. Reivindicación 118: Lasplantas Angiospermas transplastómicas establemente transformadas con cualquiera de los vectores de ADN enunciados en las reivindicaciones de la 1 a la 115. Reivindicación 122: El cultivo de las plantas transplastómicas de la reivindicación120. Reivindicación 124: La purificación y utilización de la proteína o proteínas que producen las células de las plantas de la reivindicación 120 como resultado de la expresión del gen o los genes en cuestión .
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU20020208 | 2002-09-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR041375A1 true AR041375A1 (es) | 2005-05-11 |
Family
ID=32034750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030103474A AR041375A1 (es) | 2002-09-27 | 2003-09-24 | Vector para la produccion de plantas angiospermas transplastomicas |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20060026704A1 (es) |
| EP (1) | EP1557468A2 (es) |
| JP (1) | JP2006500037A (es) |
| KR (1) | KR20050054928A (es) |
| CN (1) | CN1685047A (es) |
| AR (1) | AR041375A1 (es) |
| AU (1) | AU2003266096A1 (es) |
| BR (1) | BR0314841A (es) |
| CA (1) | CA2496913A1 (es) |
| MX (1) | MXPA05003185A (es) |
| WO (1) | WO2004029256A2 (es) |
| ZA (1) | ZA200501740B (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2573188A2 (en) | 2005-03-02 | 2013-03-27 | Metanomics GmbH | Process for the production of fine chemicals |
| AU2006219823A1 (en) | 2005-03-02 | 2006-09-08 | Metanomics Gmbh | Process for the production of fine chemicals |
| JP2006246744A (ja) * | 2005-03-09 | 2006-09-21 | Research Organization Of Information & Systems | 遺伝子導入方法、ジーンターゲッティング方法及びトランスジェニック植物の製造方法 |
| KR100740694B1 (ko) * | 2006-02-09 | 2007-07-18 | 한국생명공학연구원 | 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발 |
| GB0604966D0 (en) | 2006-03-11 | 2006-04-19 | Renovo Ltd | Medicaments and proteins |
| GB0604938D0 (en) | 2006-03-11 | 2006-04-19 | Renovo Ltd | Proteins, nucleic acids and medicaments |
| GB0604964D0 (en) | 2006-03-11 | 2006-04-19 | Renovo Ltd | Protein folding |
| GB0617816D0 (en) * | 2006-09-11 | 2006-10-18 | Renovo Ltd | Nucleic acids and methods of protein expression |
| US8044260B2 (en) * | 2007-03-09 | 2011-10-25 | Monsanto Technology Llc | Method of meristem excision and transformation |
| US20110037083A1 (en) * | 2009-01-14 | 2011-02-17 | Alex Chi Keung Chan | Led package with contrasting face |
| CN102618566B (zh) * | 2011-01-31 | 2014-01-08 | 中国科学院海洋研究所 | 一种构建亚心型扁藻叶绿体表达系统的方法 |
| CN108192897A (zh) * | 2018-02-11 | 2018-06-22 | 云南省烟草农业科学研究院 | 一种烟草rbcl基因片段及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7461987A (en) * | 1986-06-23 | 1987-12-24 | Biotechnica International Inc. | Transformation of chloroplasts |
| GEP20012558B (en) * | 1995-08-10 | 2001-10-25 | Rutgers Univ | Nuclear-Encoded Transcription System in Plastids of Higher Plants |
| US6624296B1 (en) * | 1997-06-03 | 2003-09-23 | Rutgers, The State University Of New Jersey | Plastid promoters for transgene expression in the plastids of higher plants |
-
2003
- 2003-09-15 KR KR1020057004013A patent/KR20050054928A/ko not_active Withdrawn
- 2003-09-15 JP JP2004538665A patent/JP2006500037A/ja not_active Abandoned
- 2003-09-15 WO PCT/CU2003/000009 patent/WO2004029256A2/es not_active Ceased
- 2003-09-15 CN CNA038229919A patent/CN1685047A/zh active Pending
- 2003-09-15 MX MXPA05003185A patent/MXPA05003185A/es unknown
- 2003-09-15 US US10/527,048 patent/US20060026704A1/en not_active Abandoned
- 2003-09-15 BR BR0314841-6A patent/BR0314841A/pt not_active IP Right Cessation
- 2003-09-15 AU AU2003266096A patent/AU2003266096A1/en not_active Abandoned
- 2003-09-15 CA CA002496913A patent/CA2496913A1/en not_active Abandoned
- 2003-09-15 EP EP03798037A patent/EP1557468A2/en not_active Withdrawn
- 2003-09-24 AR ARP030103474A patent/AR041375A1/es unknown
-
2005
- 2005-02-28 ZA ZA200501740A patent/ZA200501740B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004029256A3 (es) | 2004-04-29 |
| KR20050054928A (ko) | 2005-06-10 |
| AU2003266096A1 (en) | 2004-04-19 |
| ZA200501740B (en) | 2005-09-06 |
| CA2496913A1 (en) | 2004-04-08 |
| WO2004029256A2 (es) | 2004-04-08 |
| JP2006500037A (ja) | 2006-01-05 |
| MXPA05003185A (es) | 2005-06-08 |
| CN1685047A (zh) | 2005-10-19 |
| US20060026704A1 (en) | 2006-02-02 |
| BR0314841A (pt) | 2005-08-09 |
| EP1557468A2 (en) | 2005-07-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |