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AR035070A1 - Molecula aislada de adn, metodo para detectar en algodon la presencia de adn del evento pv-ghgt07(1445), equipo de deteccion de adn, metodo para cultivar una planta de algodon, metodo para determinar la cigosidad del adn y secuencia aislada de cebador de nucleotidos de adn - Google Patents

Molecula aislada de adn, metodo para detectar en algodon la presencia de adn del evento pv-ghgt07(1445), equipo de deteccion de adn, metodo para cultivar una planta de algodon, metodo para determinar la cigosidad del adn y secuencia aislada de cebador de nucleotidos de adn

Info

Publication number
AR035070A1
AR035070A1 ARP010105001A ARP010105001A AR035070A1 AR 035070 A1 AR035070 A1 AR 035070A1 AR P010105001 A ARP010105001 A AR P010105001A AR P010105001 A ARP010105001 A AR P010105001A AR 035070 A1 AR035070 A1 AR 035070A1
Authority
AR
Argentina
Prior art keywords
dna
cotton
ghgt07
event
isolated
Prior art date
Application number
ARP010105001A
Other languages
English (en)
Inventor
Tasneem S Rangwala
Minwei Ye
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR035070A1 publication Critical patent/AR035070A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proveen composiciones y métodos para detectar la presencia de la región de inserción transgénica/genómica en plantas y semillas de una planta de algodón denominada PV-GHGT07(1445). Se proveen secuencias de ADN que comprenden al menos una secuencia de región de inserción transgénica/genómica de PV-GHGT07(1445) identificada como SEQ ID N° 5 y SEQ ID N° 6, y complementos de las mismas, donde una secuencia de región de inserción abarca la unión entre el ADN heterólogo insertado en el genoma de algodón y el ADN del genoma de algodón que rodea el sitio de inserción y es diagnóstico del suceso. De acuerdo con otro aspecto se incluyen secuencias de ADN que comprenden una longitud suficiente de polinucleótidos de secuencia genómica de algodón de la planta de algodón PV-GHGT07(1445) de SEQ ID N° 7 y SEQ ID N° 8 que son útiles como secuencias cebadoras para la producción de un producto de amplicón diagnóstico para PV-GHGT07(1445). De acuerdo con otro aspecto se proveen métodos para detectar la presencia de ADN que corresponde al suceso de algodón PV-GHGT07(1445) en una muestra, métodos para producir una planta de algodón que tolera la aplicación de glifosato y métodos para determinar la cigosidad de la progenie de un cruzamiento con PV-GHGT07(1445). También se proveen equipos de detección de ADN específico para el ADN genómico/transgénico del suceso de algodón identificado como PV-GHGT07 (1445).
ARP010105001A 2000-10-25 2001-10-25 Molecula aislada de adn, metodo para detectar en algodon la presencia de adn del evento pv-ghgt07(1445), equipo de deteccion de adn, metodo para cultivar una planta de algodon, metodo para determinar la cigosidad del adn y secuencia aislada de cebador de nucleotidos de adn AR035070A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24319000P 2000-10-25 2000-10-25

Publications (1)

Publication Number Publication Date
AR035070A1 true AR035070A1 (es) 2004-04-14

Family

ID=22917694

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010105001A AR035070A1 (es) 2000-10-25 2001-10-25 Molecula aislada de adn, metodo para detectar en algodon la presencia de adn del evento pv-ghgt07(1445), equipo de deteccion de adn, metodo para cultivar una planta de algodon, metodo para determinar la cigosidad del adn y secuencia aislada de cebador de nucleotidos de adn

Country Status (5)

Country Link
US (4) US6740488B2 (es)
EP (1) EP1366070A2 (es)
AR (1) AR035070A1 (es)
AU (2) AU1536302A (es)
WO (1) WO2002034946A2 (es)

Families Citing this family (501)

* Cited by examiner, † Cited by third party
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