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AR114135A2 - Eventos de tolerancia a glifosato en alfalfa y métodos para su detección - Google Patents

Eventos de tolerancia a glifosato en alfalfa y métodos para su detección

Info

Publication number
AR114135A2
AR114135A2 ARP170101885A ARP170101885A AR114135A2 AR 114135 A2 AR114135 A2 AR 114135A2 AR P170101885 A ARP170101885 A AR P170101885A AR P170101885 A ARP170101885 A AR P170101885A AR 114135 A2 AR114135 A2 AR 114135A2
Authority
AR
Argentina
Prior art keywords
dna
alfalfa
seq
plant
sample
Prior art date
Application number
ARP170101885A
Other languages
English (en)
Inventor
Carlos Reyes
Mark Mcaslin
Sharie Fitzpatrick
Ken Ferreira
Kim A Beazley
Original Assignee
Monsanto Technology Llc
Forage Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc, Forage Genetics Inc filed Critical Monsanto Technology Llc
Publication of AR114135A2 publication Critical patent/AR114135A2/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Eventos de alfalfa J-101 y J-163, y moléculas de ADN únicas para estos eventos. La presente también provee métodos para detectar la presencia de estas moléculas de ADN en una muestra vegetal. Reivindicación 1: Semillas de plantas de alfalfa, caracterizadas porque su denominación es J-101 y sus semillas representativas están depositadas en la Colección Americana de Tipos de Cultivo (ATCC) con el Nº de Acceso PTA-4814. Reivindicación 5: La planta de alfalfa J-101 de la reivindicación 4, caracterizada porque el genoma de dicha planta de alfalfa J-101 comprende una molécula de ADN que se selecciona del grupo que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3 y SEQ ID Nº 4. Reivindicación 10: Un método para producir una planta que tolera la aplicación del herbicida glifosato, caracterizado porque comprende: (a) cruzar por vía sexual un primer progenitor de un evento de alfalfa con tolerancia a glifosato J-101, y un segundo progenitor de una planta de alfalfa que carece de tolerancia a glifosato, produciendo así una pluralidad de plantas de la progenie; y (b) seleccionar una planta de la progenie que tolera la aplicación de glifosato; y (c) autocruzar dicha planta de la primera progenie, produciendo así una pluralidad de plantas de la segunda progenie; y (d) seleccionar entre dichas plantas de la segunda progenie aquellas plantas que presentan tolerancia a glifosato. Reivindicación 11: Un método para detectar la presencia de un ADN que corresponde a ADN de la planta de alfalfa J-101 en una muestra, caracterizado porque comprende: (a) poner la muestra que comprende ADN en contacto con un conjunto de cebadores que, cuando se lo usa en una reacción de amplificación de ácidos nucleicos con ADN genómico del evento de alfalfa J-101, produce un amplicón de ADN que comprende la SEQ ID Nº 1 o SEQ ID Nº 2; y (b) realizar una reacción de amplificación de ácidos nucleicos, produciendo así el amplicón; y (c) detectar el amplicón. Reivindicación 12: Un método para detectar la presencia de un ADN que corresponde a alfalfa J-101 en una muestra, caracterizado porque comprende: (a) poner la muestra que comprende ADN en contacto con una sonda, que se hibridiza bajo condiciones de hibridación estrictas con ADN genómico del evento de alfalfa J-101 y no se hibridiza bajo condiciones de hibridación estrictas con ADN de una planta de alfalfa control, donde dicha sonda es homóloga o complementaria con SEQ ID Nº 1 o SEQ ID Nº 2; y (b) someter la muestra y la sonda a condiciones de hibridación estrictas; y (c) detectar la hibridación de la sonda con el ADN.
ARP170101885A 2003-01-31 2017-07-07 Eventos de tolerancia a glifosato en alfalfa y métodos para su detección AR114135A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44399703P 2003-01-31 2003-01-31

Publications (1)

Publication Number Publication Date
AR114135A2 true AR114135A2 (es) 2020-07-29

Family

ID=32850816

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP040100305A AR048798A1 (es) 2003-01-31 2004-02-02 Eventos de tolerancia a glifosato en alfalfa y metodos para su deteccion
ARP170101885A AR114135A2 (es) 2003-01-31 2017-07-07 Eventos de tolerancia a glifosato en alfalfa y métodos para su detección

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP040100305A AR048798A1 (es) 2003-01-31 2004-02-02 Eventos de tolerancia a glifosato en alfalfa y metodos para su deteccion

Country Status (10)

Country Link
US (5) US7566817B2 (es)
EP (3) EP2267136B1 (es)
AR (2) AR048798A1 (es)
AT (1) ATE553202T1 (es)
AU (1) AU2004209383B2 (es)
BR (2) BRPI0407173B1 (es)
CA (2) CA2514450C (es)
DK (3) DK2267136T3 (es)
MX (2) MXPA05008143A (es)
WO (1) WO2004070020A2 (es)

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PT1885176T (pt) 2005-05-27 2016-11-28 Monsanto Technology Llc Evento mon89788 de soja e métodos para a sua deteção
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CN104561262B (zh) * 2014-11-11 2017-04-19 黄埔出入境检验检疫局综合技术服务中心 转基因苜蓿草J101品系Taqman实时荧光定量PCR检测用引物和探针及检测方法
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US10357013B1 (en) 2018-03-01 2019-07-23 Forage Genetics International, Llc Alfalfa variety 412W201
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US10779504B2 (en) 2019-01-23 2020-09-22 Forage Genetics International, Llc Alfalfa variety RRL44M121
US10681894B1 (en) 2019-01-23 2020-06-16 Forage Genetics International, Llc Alfalfa variety 512W206
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US11330786B1 (en) 2020-12-23 2022-05-17 Forage Genetics International, Llc Alfalfa variety H0316ML103
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US11252927B1 (en) 2020-12-23 2022-02-22 Forage Genetics International, Llc Alfalfa variety C0516A3154
US12478028B2 (en) 2021-12-22 2025-11-25 Forage Genetics International, Llc Alfalfa variety H0815T503
US12414531B2 (en) 2021-12-22 2025-09-16 Forage Genetics International, Llc Alfalfa variety C0415C4360
US12389865B2 (en) 2021-12-22 2025-08-19 Forage Genetics International, Llc Alfalfa variety C0316ML134
US12514220B2 (en) 2021-12-22 2026-01-06 Forage Genetics International, Llc Alfalfa variety H0416A3126
US12364244B2 (en) 2022-01-28 2025-07-22 Forage Genetics International, Llc Alfalfa variety R411M104
US12127524B2 (en) 2022-01-28 2024-10-29 Forage Genetics International, Llc Alfalfa variety R414W277
US12245567B2 (en) 2022-01-28 2025-03-11 Forage Genetics International, Llc Alfalfa variety R414W279
US12336493B2 (en) 2022-01-28 2025-06-24 Forage Genetics International, Llc Alfalfa variety R914T542
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