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PE108198A1 - Lineas celulares inmortalizadas para el cultivo de virus - Google Patents

Lineas celulares inmortalizadas para el cultivo de virus

Info

Publication number
PE108198A1
PE108198A1 PE1997000704A PE00070497A PE108198A1 PE 108198 A1 PE108198 A1 PE 108198A1 PE 1997000704 A PE1997000704 A PE 1997000704A PE 00070497 A PE00070497 A PE 00070497A PE 108198 A1 PE108198 A1 PE 108198A1
Authority
PE
Peru
Prior art keywords
cells
viruses
fibroblastes
immortalized cells
immortalized
Prior art date
Application number
PE1997000704A
Other languages
English (en)
Inventor
Douglas N Foster
Linda K Foster
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of PE108198A1 publication Critical patent/PE108198A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16651—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Rheumatology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

SE REFIEREN A CELULAS INMORTALIZADAS ESPONTANEAMENTE DE FIBROBLASTOS DE EMBRIONES DE POLLO EN CULTIVO, CON LAS CARACTERISTICAS DE UNA LINEA CELULAR UMNSAH-DF1, Y A LOS SUBCLONES DE ESTAS CELULAS INMORTALIZADAS. TAMBIEN SE REFIERE A UN PROCEDIMIENTO DE PREPARACION DE CELULAS INMORTALIZADAS, QUE COMPRENDE: CULTIVAR FIBROBLASTOS PRIMARIOS DE EMBRIONES DE POLLO; TRASLADAR LOS FIBROBLASTOS EN CULTIVO HASTA QUE EMPIECE SU ENVEJECIMIENTO; CONCENTRAR LAS CELULAS DURANTE SU ENVEJECIMIENTO PARA MANTENERLAS ENTRE 30% A 60% EN EL CULTIVO; IDENTIFICAR Y AISLAR LOS FOCOS DE CELULAS NO ENVEJECIDAS; CULTIVAR LAS CELULAS NO ENVEJECIDAS EN MAS DE 30 PASOS. ADEMAS SE REFIERE AL PROCEDIMIENTO DE CULTIVO DE VIRUS EN CELULAS INMORTALIZADAS. LAS CELULAS INMORTALIZADAS ESPONTANEAMENTE SON RESERVORIOS LIBRES DE VIRUS CONTAMINANTES Y DE PROTEINA VIRAL, QUE FAVORECEN EL CULTIVO DEL VIRUS O LA EXPRESION DE LA PROTEINA O DEL VIRUS RECOMBINANTE, SIENDO UTILES PARA PRODUCIR VACUNAS PARA ANIMALES, ADEMAS PUEDEN SERVIR COMO SUSTRATO PARA APOYAR EL CRECIMIENTO DE VIRUS DE OTRAS CELULAS, QUE PERMITA LA PROPAGACION DEL VIRUS COMERCIALMENTE
PE1997000704A 1996-08-13 1997-08-13 Lineas celulares inmortalizadas para el cultivo de virus PE108198A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/696,200 US5672485A (en) 1996-08-13 1996-08-13 Immortalized cell lines for virus growth

Publications (1)

Publication Number Publication Date
PE108198A1 true PE108198A1 (es) 1999-01-21

Family

ID=24796108

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1997000704A PE108198A1 (es) 1996-08-13 1997-08-13 Lineas celulares inmortalizadas para el cultivo de virus

Country Status (28)

Country Link
US (4) US5672485A (es)
EP (1) EP0920491B1 (es)
JP (3) JP3754088B2 (es)
KR (1) KR100375871B1 (es)
CN (1) CN1228119B (es)
AR (1) AR008294A1 (es)
AT (1) ATE362524T1 (es)
AU (1) AU726820B2 (es)
BR (1) BR9711067A (es)
CA (1) CA2263725C (es)
CO (1) CO4700552A1 (es)
DE (1) DE69737735T2 (es)
DK (1) DK0920491T3 (es)
DZ (1) DZ2291A1 (es)
ES (1) ES2285738T3 (es)
GT (1) GT199700091A (es)
HN (1) HN1997000124A (es)
HU (1) HU226658B1 (es)
IL (4) IL128511A (es)
MA (1) MA24303A1 (es)
MY (1) MY125594A (es)
NZ (1) NZ334147A (es)
PE (1) PE108198A1 (es)
PT (1) PT920491E (es)
SV (1) SV1997000071A (es)
UY (1) UY24668A1 (es)
WO (1) WO1998006824A1 (es)
ZA (1) ZA977216B (es)

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US5672485A (en) * 1996-08-13 1997-09-30 Regents Of The University Of Minnesota Immortalized cell lines for virus growth

Also Published As

Publication number Publication date
CA2263725A1 (en) 1998-02-19
ES2285738T3 (es) 2007-11-16
KR100375871B1 (ko) 2003-03-10
IL158303A0 (en) 2004-05-12
BR9711067A (pt) 1999-08-17
JP3754088B2 (ja) 2006-03-08
HUP0000188A2 (hu) 2000-06-28
US5672485A (en) 1997-09-30
US5985642A (en) 1999-11-16
AU4069197A (en) 1998-03-06
EP0920491A1 (en) 1999-06-09
US6207415B1 (en) 2001-03-27
PT920491E (pt) 2007-07-27
DE69737735D1 (de) 2007-06-28
ATE362524T1 (de) 2007-06-15
JP2006320328A (ja) 2006-11-30
EP0920491B1 (en) 2007-05-16
HU226658B1 (en) 2009-06-29
MA24303A1 (fr) 1998-04-01
CN1228119B (zh) 2012-04-04
IL128511A0 (en) 2000-01-31
GT199700091A (es) 1997-08-12
IL158302A0 (en) 2004-05-12
IL128511A (en) 2004-06-01
HK1022496A1 (en) 2000-08-11
ZA977216B (en) 1998-02-19
DK0920491T3 (da) 2007-09-17
DE69737735T2 (de) 2008-01-31
US5879924A (en) 1999-03-09
KR20000029968A (ko) 2000-05-25
NZ334147A (en) 2000-08-25
AR008294A1 (es) 1999-12-29
IL158301A0 (en) 2004-05-12
HUP0000188A3 (en) 2005-11-28
WO1998006824A1 (en) 1998-02-19
HN1997000124A (es) 1999-03-19
SV1997000071A (es) 2000-07-06
JP2000516095A (ja) 2000-12-05
CN1228119A (zh) 1999-09-08
CO4700552A1 (es) 1998-12-29
MY125594A (en) 2006-08-30
UY24668A1 (es) 1998-02-02
DZ2291A1 (fr) 2002-12-25
AU726820B2 (en) 2000-11-23
CA2263725C (en) 2002-10-15
JP2005118053A (ja) 2005-05-12

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