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UY24668A1 - Metodo para producir lineas de celulas inmortalizadas para crecimiento de virus - Google Patents

Metodo para producir lineas de celulas inmortalizadas para crecimiento de virus

Info

Publication number
UY24668A1
UY24668A1 UY24668A UY24668A UY24668A1 UY 24668 A1 UY24668 A1 UY 24668A1 UY 24668 A UY24668 A UY 24668A UY 24668 A UY24668 A UY 24668A UY 24668 A1 UY24668 A1 UY 24668A1
Authority
UY
Uruguay
Prior art keywords
cells
culture
virus
unaged
cell
Prior art date
Application number
UY24668A
Other languages
English (en)
Inventor
N Foster Douglas
K Foster Linda
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of UY24668A1 publication Critical patent/UY24668A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16651—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Rheumatology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Método para producir una línea de células inmortalizadas de fibroblastos embriónicos de pollo comprendiendo los pasos de: hacer fibroblastos primarios de pollo en cultivo; pasando los fibroblastos en cultivo hasta iniciar el envejecimiento de la célula; concentrando las células durante el envejecimiento de las células para mantener de 30 a 60% de la confluencia el cultivo; identificando el foci de las células no envejecidas; y haciendo crecer las células no envejecidas por más de 30 pasajes. En particular se reivindica el método donde las líneas de células son UMNSAH y depositadas en la colección Americana de Cultivos tipo como accesión NºCRL-12203, en el cultivo; infectar las células con el virus; permitir que el virus repliegue en las células; y recoger el virus que replegó las células.
UY24668A 1996-08-13 1997-08-13 Metodo para producir lineas de celulas inmortalizadas para crecimiento de virus UY24668A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/696,200 US5672485A (en) 1996-08-13 1996-08-13 Immortalized cell lines for virus growth

Publications (1)

Publication Number Publication Date
UY24668A1 true UY24668A1 (es) 1998-02-02

Family

ID=24796108

Family Applications (1)

Application Number Title Priority Date Filing Date
UY24668A UY24668A1 (es) 1996-08-13 1997-08-13 Metodo para producir lineas de celulas inmortalizadas para crecimiento de virus

Country Status (28)

Country Link
US (4) US5672485A (es)
EP (1) EP0920491B1 (es)
JP (3) JP3754088B2 (es)
KR (1) KR100375871B1 (es)
CN (1) CN1228119B (es)
AR (1) AR008294A1 (es)
AT (1) ATE362524T1 (es)
AU (1) AU726820B2 (es)
BR (1) BR9711067A (es)
CA (1) CA2263725C (es)
CO (1) CO4700552A1 (es)
DE (1) DE69737735T2 (es)
DK (1) DK0920491T3 (es)
DZ (1) DZ2291A1 (es)
ES (1) ES2285738T3 (es)
GT (1) GT199700091A (es)
HN (1) HN1997000124A (es)
HU (1) HU226658B1 (es)
IL (4) IL158302A0 (es)
MA (1) MA24303A1 (es)
MY (1) MY125594A (es)
NZ (1) NZ334147A (es)
PE (1) PE108198A1 (es)
PT (1) PT920491E (es)
SV (1) SV1997000071A (es)
UY (1) UY24668A1 (es)
WO (1) WO1998006824A1 (es)
ZA (1) ZA977216B (es)

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JPWO2004031450A1 (ja) * 2002-09-30 2006-02-02 本田技研工業株式会社 水素活性化装置
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US20070190601A1 (en) * 2004-10-08 2007-08-16 Avigenics, Inc. Modified integrase and methods of use
US8137907B2 (en) * 2005-01-03 2012-03-20 Cold Spring Harbor Laboratory Orthotopic and genetically tractable non-human animal model for liver cancer and the uses thereof
FR2884255B1 (fr) 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
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ES2671230T3 (es) 2006-06-20 2018-06-05 Transgene Sa Procedimiento para producir poxvirus y composiciones de poxvirus
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JP7712284B2 (ja) 2020-03-12 2025-07-23 バヴァリアン・ノルディック・アクティーゼルスカブ ポックスウイルスの安定性を改善する組成物
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US5672485A (en) * 1996-08-13 1997-09-30 Regents Of The University Of Minnesota Immortalized cell lines for virus growth

Also Published As

Publication number Publication date
JP3754088B2 (ja) 2006-03-08
IL158302A0 (en) 2004-05-12
US6207415B1 (en) 2001-03-27
KR100375871B1 (ko) 2003-03-10
EP0920491B1 (en) 2007-05-16
CA2263725C (en) 2002-10-15
IL158301A0 (en) 2004-05-12
HUP0000188A2 (hu) 2000-06-28
HK1022496A1 (en) 2000-08-11
CO4700552A1 (es) 1998-12-29
HUP0000188A3 (en) 2005-11-28
US5672485A (en) 1997-09-30
WO1998006824A1 (en) 1998-02-19
CN1228119B (zh) 2012-04-04
PT920491E (pt) 2007-07-27
MA24303A1 (fr) 1998-04-01
DZ2291A1 (fr) 2002-12-25
CN1228119A (zh) 1999-09-08
IL128511A0 (en) 2000-01-31
DE69737735T2 (de) 2008-01-31
AU726820B2 (en) 2000-11-23
AR008294A1 (es) 1999-12-29
DE69737735D1 (de) 2007-06-28
ATE362524T1 (de) 2007-06-15
JP2000516095A (ja) 2000-12-05
ZA977216B (en) 1998-02-19
HN1997000124A (es) 1999-03-19
BR9711067A (pt) 1999-08-17
NZ334147A (en) 2000-08-25
MY125594A (en) 2006-08-30
IL128511A (en) 2004-06-01
JP2006320328A (ja) 2006-11-30
JP2005118053A (ja) 2005-05-12
PE108198A1 (es) 1999-01-21
KR20000029968A (ko) 2000-05-25
IL158303A0 (en) 2004-05-12
ES2285738T3 (es) 2007-11-16
HU226658B1 (en) 2009-06-29
EP0920491A1 (en) 1999-06-09
SV1997000071A (es) 2000-07-06
US5879924A (en) 1999-03-09
AU4069197A (en) 1998-03-06
DK0920491T3 (da) 2007-09-17
GT199700091A (es) 1997-08-12
US5985642A (en) 1999-11-16
CA2263725A1 (en) 1998-02-19

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