DE60044728D1 - VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE - Google Patents

VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE

Info

Publication number: DE60044728D1
Authority: DE; Germany
Prior art keywords: insulin; analogs; forecasts; exports; yeast
Prior art date: 1999-12-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE60044728T

Other languages

English (en)

Inventor

Thomas Borglum Kjeldsen

Svend Ludvigsen

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novo Nordisk AS

Original Assignee

Novo Nordisk AS

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-12-29

Filing date

2000-12-04

Publication date

2010-09-02

2000-12-04 Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS

2010-09-02 Application granted granted Critical

2010-09-02 Publication of DE60044728D1 publication Critical patent/DE60044728D1/de

2020-12-05 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 8
102000004877 Insulin Human genes 0.000 title abstract 4
108090001061 Insulin Proteins 0.000 title abstract 4
229940125396 insulin Drugs 0.000 title abstract 4
240000004808 Saccharomyces cerevisiae Species 0.000 title 1
238000000855 fermentation Methods 0.000 title 1
230000004151 fermentation Effects 0.000 title 1
238000004519 manufacturing process Methods 0.000 title 1
238000000034 method Methods 0.000 title 1
239000002243 precursor Substances 0.000 abstract 4
108010075254 C-Peptide Proteins 0.000 abstract 2
PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 2
VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 abstract 1
101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
125000000539 amino acid group Chemical group 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Gastroenterology & Hepatology (AREA)
Endocrinology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Toxicology (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Diabetes (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

DE60044728T 1999-12-29 2000-12-04 VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE Expired - Lifetime DE60044728D1 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
DKPA199901869		1999-12-29
DKPA200000443		2000-03-17
PCT/DK2000/000664 WO2001049870A1 (en)	1999-12-29	2000-12-04	Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast

Publications (1)

Publication Number	Publication Date
DE60044728D1 true DE60044728D1 (de)	2010-09-02

Family

ID=26066173

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE60044728T Expired - Lifetime DE60044728D1 (de)	1999-12-29	2000-12-04	VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE

Country Status (13)

Country	Link
EP (1)	EP1246933B1 (de)
JP (1)	JP4795599B2 (de)
KR (1)	KR20030004315A (de)
CN (1)	CN1415019A (de)
AT (1)	ATE474930T1 (de)
AU (1)	AU1995701A (de)
BR (1)	BR0016860A (de)
CA (1)	CA2392844A1 (de)
DE (1)	DE60044728D1 (de)
HU (1)	HUP0301640A2 (de)
PL (1)	PL356005A1 (de)
RU (1)	RU2002120513A (de)
WO (1)	WO2001049870A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
BRPI0009001B1 (pt)	1999-03-16	2017-06-06	Novozymes As	processo para a produção de queijo
US6777207B2 (en)	1999-12-29	2004-08-17	Novo Nordisk A/S	Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US7378390B2 (en)	1999-12-29	2008-05-27	Novo Nordisk A/S	Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
WO2002079251A2 (en) *	2001-04-02	2002-10-10	Novo Nordisk A/S	Method for making human insulin precursors
DE60233722D1 (de) *	2001-04-02	2009-10-29	Novo Nordisk As	Insulinvorstufen und verfahren zu deren herstellung
AU2002325088C1 (en) *	2001-09-14	2008-09-25	Clinical Genomics Pty. Ltd.	Nucleic acid markers for use in determining predisposition to neoplasm and/or adenoma
ATE464390T1 (de)	2001-11-19	2010-04-15	Novo Nordisk As	Verfahren zur herstellung von insulinverbindungen
JP5697831B2 (ja)	2003-12-03	2015-04-08	ノヴォノルディスクアー／エス	単鎖インシュリン
CN100460508C (zh) *	2004-11-03	2009-02-11	马延高	人胰岛素基因在甲醇酵母中的分泌表达方法
EP1917363B1 (de) *	2005-08-16	2011-06-22	Novo Nordisk A/S	Verfahren zur herstellung reifer insulinpolypeptide
WO2008037735A1 (en)	2006-09-27	2008-04-03	Novo Nordisk A/S	Method for making maturated insulin polypeptides
WO2008049711A1 (en)	2006-10-27	2008-05-02	Novo Nordisk A/S	Peptide extended insulins
CN105418755A (zh) *	2015-12-28	2016-03-23	珠海冀百康生物科技有限公司	速效胰岛素前体蛋白以及速效胰岛素的制备方法
CN111560379B (zh) *	2020-05-26	2022-09-13	北京惠之衡生物科技有限公司	密码子优化的门冬胰岛素前体基因、重组载体、基因工程菌及其应用
CN114380903B (zh) *	2021-12-28	2023-07-25	上海仁会生物制药股份有限公司	一种胰岛素或其类似物前体

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4343898A (en)	1980-02-11	1982-08-10	Novo Industri A/S	Process for preparing esters of human insulin
NZ199391A (en) *	1981-01-02	1985-12-13	Genentech Inc	Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US4546082A (en)	1982-06-17	1985-10-08	Regents Of The Univ. Of California	E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4870008A (en)	1983-08-12	1989-09-26	Chiron Corporation	Secretory expression in eukaryotes
AU600885B2 (en)	1984-05-25	1990-08-30	Zymogenetics Inc.	Stable DNA constructs
US5871957A (en)	1984-05-25	1999-02-16	Zymogenetics, Inc.	Stable DNA constructs
DK58285D0 (da)	1984-05-30	1985-02-08	Novo Industri As	Peptider samt fremstilling og anvendelse deraf
DK463887D0 (da)	1987-09-07	1987-09-07	Novo Industri As	Gaerleader
DK336188D0 (da)	1988-06-20	1988-06-20	Nordisk Gentofte	Propeptider
DK105489D0 (da)	1989-03-03	1989-03-03	Novo Nordisk As	Polypeptid
DK300090D0 (da)	1990-12-19	1990-12-19	Novo Nordisk As	Fremgangsmaade til fremstilling af leadersekvenser
DK82893D0 (da)	1993-07-08	1993-07-08	Novo Nordisk As	Peptid
AU1272295A (en)	1993-12-17	1995-07-03	Novo Nordisk A/S	Proinsulin-like compounds
US5639642A (en)	1994-06-16	1997-06-17	Novo Nordisk A/S	Synthetic leader peptide sequences
ZA954983B (en)	1994-06-17	1996-02-14	Novo Nordisk As	N-terminally extended proteins expressed in yeast
KR0150565B1 (ko) *	1995-02-15	1998-08-17	김정재	유전자 조환에 의한 사람 인슐린 전구체의 제조 및 이를 이용한 인슐린의 제조방법
IL118127A0 (en)	1995-05-05	1996-09-12	Lilly Co Eli	Single chain insulin with high bioactivity
WO1998032867A1 (en)	1997-01-24	1998-07-30	Novo Nordisk A/S	Synthetic leader peptide sequences

2000
- 2000-12-04 KR KR1020027008586A patent/KR20030004315A/ko not_active Withdrawn
- 2000-12-04 HU HU0301640A patent/HUP0301640A2/hu unknown
- 2000-12-04 AU AU19957/01A patent/AU1995701A/en not_active Abandoned
- 2000-12-04 RU RU2002120513/13A patent/RU2002120513A/ru not_active Application Discontinuation
- 2000-12-04 BR BR0016860-2A patent/BR0016860A/pt not_active IP Right Cessation
- 2000-12-04 AT AT00983074T patent/ATE474930T1/de not_active IP Right Cessation
- 2000-12-04 WO PCT/DK2000/000664 patent/WO2001049870A1/en not_active Ceased
- 2000-12-04 CA CA002392844A patent/CA2392844A1/en not_active Abandoned
- 2000-12-04 JP JP2001550398A patent/JP4795599B2/ja not_active Expired - Fee Related
- 2000-12-04 DE DE60044728T patent/DE60044728D1/de not_active Expired - Lifetime
- 2000-12-04 EP EP00983074A patent/EP1246933B1/de not_active Expired - Lifetime
- 2000-12-04 CN CN00817961A patent/CN1415019A/zh active Pending
- 2000-12-04 PL PL00356005A patent/PL356005A1/xx not_active Application Discontinuation

Also Published As

Publication number	Publication date
BR0016860A (pt)	2002-10-01
KR20030004315A (ko)	2003-01-14
CN1415019A (zh)	2003-04-30
CA2392844A1 (en)	2001-07-12
PL356005A1 (en)	2004-05-31
ATE474930T1 (de)	2010-08-15
HUP0301640A2 (hu)	2003-08-28
JP2003518950A (ja)	2003-06-17
EP1246933B1 (de)	2010-07-21
JP4795599B2 (ja)	2011-10-19
AU1995701A (en)	2001-07-16
EP1246933A1 (de)	2002-10-09
RU2002120513A (ru)	2004-03-27
WO2001049870A1 (en)	2001-07-12

Publication	Publication Date	Title
DE60044728D1 (de)	2010-09-02	VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE
DE3583824D1 (de)	1991-09-26	Vorlaeufer des insulins, verfahren zu seiner herstellung und verfahren zur herstellung von humaninsulin aus solchen vorlaeufern des insulins.
DE50014830D1 (de)	2008-01-17	C-peptid zur verbesserten herstellung von insulin und insulinanaloga
DE60039074D1 (de)	2008-07-10	Verfahren zur herstellung von insulinvorläufern und von analogen von insulinvorläufern
DE69020794D1 (de)	1995-08-17	Enzymatische Entfernung einer aminoterminalen Proteinsequenz.
EP0821006A3 (de)	2001-11-28	Insulinderivate mit erhöhter Zinkbindung
DE59608521D1 (de)	2002-01-31	Verfahren zur herstellung von o-acetylserin, l-cystein und l-cystein-verwandten produkten
ES2044841T3 (es)	1994-01-16	Procedimiento de preparacion de peptidos sinteticos con actividad de interleuquin 1 humana.
WO1996011949A3 (en)	1996-12-12	Analogs of keratinocyte growth factor
DE60233722D1 (de)	2009-10-29	Insulinvorstufen und verfahren zu deren herstellung
IL127583A0 (en)	1999-10-28	Method for the production of polypeptides
DE60226323D1 (de)	2008-06-12	Peptid und dessen kosmetische oder dermatologische verwendung zur verbesserung der haftung zwischen hautzellen
ATE47880T1 (de)	1989-11-15	Vorlaeufer eines an dem c-ende amidierten peptids und dessen herstellung.
DE59108392D1 (de)	1997-01-16	Enzymatisches Verfahren zur Umwandlung von Präproinsulinen zu Insulinen
DE3781832D1 (de)	1992-10-29	C-terminal-alpha-amidierendes enzym und verfahren zu dessen herstellung und verwendung.
WO2002079251A3 (en)	2003-04-10	Method for making human insulin precursors
ATE341630T1 (de)	2006-10-15	Verwendung von pankreatischer procarboxypeptidase b zur herstellung von insulin
DE59503776D1 (de)	1998-11-05	Cyclohexapeptide und deren Mischungen, Verfahren zur Herstellung sowie deren Verwendung
EP0191869A4 (de)	1988-06-27	Fisch-kalzitoninabkömmling und dessen herstellung und gensystem dafür.