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DE60001731D1 - Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen - Google Patents

Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen

Info

Publication number
DE60001731D1
DE60001731D1 DE60001731T DE60001731T DE60001731D1 DE 60001731 D1 DE60001731 D1 DE 60001731D1 DE 60001731 T DE60001731 T DE 60001731T DE 60001731 T DE60001731 T DE 60001731T DE 60001731 D1 DE60001731 D1 DE 60001731D1
Authority
DE
Germany
Prior art keywords
particles
numbers
photon counts
fluorescent molecules
photon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60001731T
Other languages
English (en)
Other versions
DE60001731T2 (de
Inventor
Kauko Palo
Pete Kask
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Evotec OAI AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP99112104A external-priority patent/EP1063502A1/de
Priority claimed from US09/338,439 external-priority patent/US6376843B1/en
Application filed by Evotec OAI AG filed Critical Evotec OAI AG
Publication of DE60001731D1 publication Critical patent/DE60001731D1/de
Application granted granted Critical
Publication of DE60001731T2 publication Critical patent/DE60001731T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/457—Correlation spectrometry, e.g. of the intensity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J2003/2866—Markers; Calibrating of scan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)
DE60001731T 1999-04-29 2000-04-29 Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen Expired - Lifetime DE60001731T2 (de)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US13165799P 1999-04-29 1999-04-29
US131657P 1999-04-29
US338439 1999-06-23
EP99112104A EP1063502A1 (de) 1999-06-23 1999-06-23 Verfahren zur Charakterisierung fluoreszierender Moleküle oder anderer Teilchen unter Verwendung von erzeugenden Funktionen
EP99112104 1999-06-23
US09/338,439 US6376843B1 (en) 1999-06-23 1999-06-23 Method of characterizing fluorescent molecules or other particles using generating functions
EP99125745 1999-12-23
EP99125745 1999-12-23
US18156400P 2000-02-10 2000-02-10
US181564P 2000-02-10
PCT/EP2000/003890 WO2000066985A1 (en) 1999-04-29 2000-04-29 A method of characterizing fluorescent molecules or other particles using generating functions

Publications (2)

Publication Number Publication Date
DE60001731D1 true DE60001731D1 (de) 2003-04-24
DE60001731T2 DE60001731T2 (de) 2004-02-05

Family

ID=56290015

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60001731T Expired - Lifetime DE60001731T2 (de) 1999-04-29 2000-04-29 Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen

Country Status (7)

Country Link
EP (1) EP1175602B1 (de)
JP (1) JP3559525B2 (de)
AT (1) ATE235045T1 (de)
AU (1) AU4559600A (de)
DE (1) DE60001731T2 (de)
DK (1) DK1175602T3 (de)
WO (1) WO2000066985A1 (de)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6690463B2 (en) * 2000-02-10 2004-02-10 Evotec Biosystems Ag Fluorescence intensity and lifetime distribution analysis
CA2440183A1 (en) * 2001-03-09 2002-09-19 Gnothis Holding Sa Determination of analytes by means of fluorescence correlation spectroscopy
DE10117723A1 (de) * 2001-04-09 2002-10-17 Evotec Ag Probenträger, insbesondere für biochemische Reaktionen
US6658143B2 (en) * 2002-04-29 2003-12-02 Amersham Biosciences Corp. Ray-based image analysis for biological specimens
WO2005030947A2 (en) 2003-09-30 2005-04-07 Evotec Neurosciences Gmbh Diagnostic and therapeutic use of a sulfotransferase for neurodegenerative diseases
JP3793531B2 (ja) 2003-10-07 2006-07-05 オリンパス株式会社 蛍光寿命測定装置
WO2006015976A1 (en) 2004-08-11 2006-02-16 Evotec Neurosciences Gmbh Diagnostic and therapeutic use of a plasma membrane atpase
EP1835283A4 (de) * 2004-12-10 2008-05-07 Sysmex Corp Verfahren zur unterstützung der diagnose einer immunsystemfehlfunktionskrankheit und vorrichtung zur ausgabe von informationen zur unterstützung der diagnose
JP2008545402A (ja) 2005-05-27 2008-12-18 エボテツク・ニユーロサイエンシーズ・ゲー・エム・ベー・ハー 神経変性疾患の診断及び治療ターゲットとしてのｋｃｎｎ３
WO2006128879A1 (en) 2005-05-30 2006-12-07 Takeda Pharmaceutical Company Limited Diagnostic and therapeutic target prkx proteins for neurodegenerative diseases
EP1891234B1 (de) 2005-06-01 2014-12-10 Evotec International GmbH Verwendung von slc39a12-proteinen als zielmoleküle in der diagnose von und beim screening von medikamenten zur behandlung von alzheimer-krankheit
DE602006019582D1 (de) 2005-06-16 2011-02-24 Evotec Neurosciences Gmbh Adarb2 proteine als diagnostische und therapeutische targets für neurodegenerative erkrankungen
JP2009278866A (ja) * 2006-09-04 2009-12-03 Olympus Corp Ｄｎａに於けるｄｎａ結合性タンパク質の結合部位の検出方法
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen
WO2010090872A2 (en) 2009-01-21 2010-08-12 Rare Light, Inc. Correlation interferometric methods, devices and systems for low cost and rugged spectroscopy
WO2011108371A1 (ja) 2010-03-01 2011-09-09 オリンパス株式会社 光分析装置、光分析方法並びに光分析用コンピュータプログラム
CN103026205B (zh) 2010-07-26 2016-03-23 奥林巴斯株式会社 使用发光探针检测溶液中稀疏颗粒的方法
CN103097878B (zh) 2010-09-10 2015-07-22 奥林巴斯株式会社 使用单个发光颗粒的光强度的光学分析方法
WO2012032955A1 (ja) 2010-09-10 2012-03-15 オリンパス株式会社 複数の波長帯域の光計測を用いた光分析方法
JP5737704B2 (ja) * 2010-09-27 2015-06-17 株式会社ライフテック 蛍光一粒子検出方法および検出システム
EP2620763A4 (de) 2010-10-19 2015-05-27 Olympus Corp Optische analyse zum beobachten von polarisationsdaten einzelner lichtemittierender teilchen, verfahren für optische analysen und computerprogramm für optische analysen
CN103229042B (zh) 2010-11-25 2016-06-29 奥林巴斯株式会社 利用单个发光粒子的光的波长特性的光分析装置和光分析方法
CN103328955B (zh) 2011-01-20 2017-10-20 奥林巴斯株式会社 利用来自单个发光粒子的光的检测的光分析方法和光分析装置
JP6009944B2 (ja) 2011-01-26 2016-10-19 オリンパス株式会社 核酸分子の多型識別方法
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WO2012133292A1 (ja) 2011-03-29 2012-10-04 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法並びに光分析用コンピュータプログラム
EP2706346A4 (de) 2011-04-13 2014-11-19 Olympus Corp Photoanalysevorrichtung mit erkennung einzelner lichtemittierender teilchen, photoanalyseverfahren und computerprogramm zur photoanalyse
CN103620389B (zh) 2011-04-18 2016-08-17 奥林巴斯株式会社 目标粒子的定量方法、光分析装置以及光分析用计算机程序
CN103733047B (zh) 2011-08-11 2016-03-30 奥林巴斯株式会社 目标粒子的检测方法
CN103733049B (zh) 2011-08-15 2016-01-20 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
JP6010033B2 (ja) 2011-08-26 2016-10-19 オリンパス株式会社 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム
JP6013338B2 (ja) 2011-08-26 2016-10-25 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
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WO2013069504A1 (ja) 2011-11-10 2013-05-16 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
DE102012100098B4 (de) * 2012-01-06 2021-09-16 Becker & Hickl Gmbh Verfahren zur Aufzeichnung von zeitlichen Änderungen der Zeitfunktion eines optischen Signals mit räumlicher Auflösung entlang einer Linie im Raum
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WO2015015951A1 (ja) 2013-07-31 2015-02-05 オリンパス株式会社 単一発光粒子検出技術を用いた光学顕微鏡装置、顕微鏡観察法及び顕微鏡観察のためのコンピュータプログラム
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WO2017098597A1 (ja) 2015-12-09 2017-06-15 オリンパス株式会社 単一発光粒子検出を用いた光分析方法及び光分析装置
DE102019000066B4 (de) * 2019-01-09 2020-10-08 Becker & Hickl Gmbh Verfahren zur Visualisierung von Fluoreszenz-Lifetime-Imaging-Daten
US20230314221A1 (en) * 2020-08-27 2023-10-05 The Research Foundation For The State University Of New York System and method for embedded diffuse correlation spectroscopy
CN115048835B (zh) * 2022-06-09 2024-10-18 山西大学 一种等离子体中粒子瞬态分布的成像方法
KR102593306B1 (ko) * 2023-03-21 2023-10-25 부경대학교 산학협력단 저투수성 매체 내 형광 추적자 농도분포 추정 장치 및 방법
JP7739535B1 (ja) * 2024-06-19 2025-09-16 浜松ホトニクス株式会社 信号処理方法、信号処理装置、及び信号処理システム

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1595422A (en) * 1977-04-28 1981-08-12 Nat Res Dev Scaning microscopes
US5866911A (en) * 1994-07-15 1999-02-02 Baer; Stephen C. Method and apparatus for improving resolution in scanned optical system
EP0836090A1 (de) * 1996-10-12 1998-04-15 Evotec BioSystems GmbH Probenanalyseverfahren durch Bestimmung der spezifischen Helligkeitsverteilung von Teilchen
JP3830167B2 (ja) * 1996-11-27 2006-10-04 マックス−プランク−ゲゼルシャフト ツル フェルデルング デァ ヴィッセンシャフテン エー．フォオ．，ベルリン 試料媒体にある標的粒子の所定の特性を決定するための方法および装置

Also Published As

Publication number Publication date
JP3559525B2 (ja) 2004-09-02
EP1175602B1 (de) 2003-03-19
WO2000066985A1 (en) 2000-11-09
AU4559600A (en) 2000-11-17
WO2000066985A9 (en) 2002-02-14
DK1175602T3 (da) 2003-05-26
EP1175602A1 (de) 2002-01-30
DE60001731T2 (de) 2004-02-05
JP2002543414A (ja) 2002-12-17
ATE235045T1 (de) 2003-04-15

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Owner name: OLYMPUS CORPORATION, TOKIO/TOKYO, JP

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Representative=s name: PATENTANWAELTE SCHAEFER EMMEL HAUSFELD, 22043 HAMB