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BR9908858A - Fragmento de ácido nucléico isolado, gene quimérico, célula hospedeira transformada, polipeptìdio, método de alteração do nìvel de expressão de uma proteìna, método de obtenção de um fragmento de ácido nucléico e produto. - Google Patents

Fragmento de ácido nucléico isolado, gene quimérico, célula hospedeira transformada, polipeptìdio, método de alteração do nìvel de expressão de uma proteìna, método de obtenção de um fragmento de ácido nucléico e produto.

Info

Publication number
BR9908858A
BR9908858A BR9908858-4A BR9908858A BR9908858A BR 9908858 A BR9908858 A BR 9908858A BR 9908858 A BR9908858 A BR 9908858A BR 9908858 A BR9908858 A BR 9908858A
Authority
BR
Brazil
Prior art keywords
nucleic acid
protein
fragment
chimeric gene
host cell
Prior art date
Application number
BR9908858-4A
Other languages
English (en)
Inventor
Robert F Cressmann
Stephen M Allen
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9908858A publication Critical patent/BR9908858A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

<B>"FRAGMENTO DE áCIDO NUCLéICO ISOLADO, GENE QUIMéRICO, CéLULA HOSPEDEIRA TRANSFORMADA, POLIPEPTìDIO, MéTODO DE ALTERAçãO DO NìVEL DE EXPRESSãO DE UMA PROTEìNA, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO E PRODUTO"<D> A presente invenção refere-se a um fragmento de ácido nucléico isolado que codifica uma proteína de fosforilação R1 de amido. A invenção também se refere à construção de um gene quimérico que codifica toda ou uma parte da proteína de fosforilação R1 de amido, na orientação de sentido ou anti-sentido, sendo que a expressão do gene quimérico resulta na produção de níveis alterados da proteína de fosforilação R1 de amido em uma célula hospedeira transformada.
BR9908858-4A 1998-04-09 1999-04-08 Fragmento de ácido nucléico isolado, gene quimérico, célula hospedeira transformada, polipeptìdio, método de alteração do nìvel de expressão de uma proteìna, método de obtenção de um fragmento de ácido nucléico e produto. BR9908858A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8114398P 1998-04-09 1998-04-09
PCT/US1999/007639 WO1999053072A1 (en) 1998-04-09 1999-04-08 Starch r1 phosphorylation protein homologs

Publications (1)

Publication Number Publication Date
BR9908858A true BR9908858A (pt) 2000-12-19

Family

ID=22162353

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9908858-4A BR9908858A (pt) 1998-04-09 1999-04-08 Fragmento de ácido nucléico isolado, gene quimérico, célula hospedeira transformada, polipeptìdio, método de alteração do nìvel de expressão de uma proteìna, método de obtenção de um fragmento de ácido nucléico e produto.

Country Status (4)

Country Link
EP (1) EP1068333A1 (pt)
AU (1) AU3478499A (pt)
BR (1) BR9908858A (pt)
WO (1) WO1999053072A1 (pt)

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