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AR131904A2 - Secuencias de nucleótidos mutadas aisladas que codifican una proteína oleato desaturasa modificada de girasol, proteína modificada, métodos y usos - Google Patents

Secuencias de nucleótidos mutadas aisladas que codifican una proteína oleato desaturasa modificada de girasol, proteína modificada, métodos y usos

Info

Publication number
AR131904A2
AR131904A2 ARP240100392A ARP240100392A AR131904A2 AR 131904 A2 AR131904 A2 AR 131904A2 AR P240100392 A ARP240100392 A AR P240100392A AR P240100392 A ARP240100392 A AR P240100392A AR 131904 A2 AR131904 A2 AR 131904A2
Authority
AR
Argentina
Prior art keywords
modified
protein
nucleotide sequences
oleate desaturase
methods
Prior art date
Application number
ARP240100392A
Other languages
English (en)
Inventor
Alberto Javier Leon
Andrs Daniel Zambelli
Roberto Juan Reid
Mnica Mariel Morata
Marcos Kaspar
Enrique Martinez-Force
Rafael Garces
Joaqun J Salas
Monica Venegas-Caleron
Original Assignee
Advanta Semillas S A I C
Consejo Superior Investigacion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanta Semillas S A I C, Consejo Superior Investigacion filed Critical Advanta Semillas S A I C
Publication of AR131904A2 publication Critical patent/AR131904A2/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1464Helianthus annuus [sunflower]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19006DELTA12-fatty-acid desaturase (1.14.19.6), i.e. oleoyl-CoA DELTA12 desaturase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención se relaciona con secuencias de nucleótidos aisladas que comprenden una mutación puntual y donde las secuencias codifican una proteína oleato desaturasa modificada de girasol. La proteína oleato desaturasa de girasol modificada comprende al menos una de las siguientes sustituciones de aminoácidos: a) Gly por Asp en la posición 103, b) Ser por Leu en la posición 131; c) Ser por Asn en la posición 135; d) Gly por Glu en la posición 144; e) Gly por Glu en la posición 226; f) Pro por Ser en la posición 253; g) Leu por Phe en la posición 267; h) Pro por Ser en la posición 275; o es una oleato desaturasa truncada.
ARP240100392A 2011-07-01 2024-02-21 Secuencias de nucleótidos mutadas aisladas que codifican una proteína oleato desaturasa modificada de girasol, proteína modificada, métodos y usos AR131904A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2011/061164 WO2013004280A1 (en) 2011-07-01 2011-07-01 Isolated mutated nucleotide sequences that encode a modified oleate destaurase sunflower protein, modified protein, methods and uses

Publications (1)

Publication Number Publication Date
AR131904A2 true AR131904A2 (es) 2025-05-14

Family

ID=44629365

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP120102375A AR087014A1 (es) 2011-07-01 2012-06-29 Secuencias de nucleotidos mutadas aisladas que codifican una proteina oleato desaturasa modificada de girasol, proteina modificada, metodos y usos
ARP240100392A AR131904A2 (es) 2011-07-01 2024-02-21 Secuencias de nucleótidos mutadas aisladas que codifican una proteína oleato desaturasa modificada de girasol, proteína modificada, métodos y usos

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP120102375A AR087014A1 (es) 2011-07-01 2012-06-29 Secuencias de nucleotidos mutadas aisladas que codifican una proteina oleato desaturasa modificada de girasol, proteina modificada, metodos y usos

Country Status (2)

Country Link
AR (2) AR087014A1 (es)
WO (1) WO2013004280A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3099178A1 (fr) 2019-07-24 2021-01-29 Soltis Tournesol à teneur élevée en acide oléique et procédé d’obtention
EP4611532A1 (en) 2022-11-02 2025-09-10 KWS SAAT SE & Co. KGaA Oleic acid sunflower mutants and methods for detection

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5407594A (en) 1992-11-17 1994-06-08 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6342658B1 (en) * 1995-12-14 2002-01-29 Cargill, Incorporated Fatty acid desaturases and mutant sequences thereof
AR032701A1 (es) 2001-02-16 2003-11-19 Consejo Superior Investigacion Una planta, semillas y un aceite con contenido aumentado de triacilgliceroles saturados y un aceite que tiene un elevado contenido de acido estearico
US20090158452A1 (en) * 2001-12-04 2009-06-18 Johnson Richard G Transgenic plants with enhanced agronomic traits
EP1591536A1 (en) 2004-04-29 2005-11-02 Institut National de la Recherche Agronomique Method of selecting sunflower genotypes with high oleic acid content in seed oil
WO2006024351A1 (en) 2004-07-30 2006-03-09 Basf Agrochemical Products B.V. Herbicide-resistant sunflower plants, plynucleotides encoding herbicide-resistant acetohydroxy acid synthase large subunit proteins, and methods of use
AU2006294546B2 (en) * 2005-09-28 2012-08-09 Dow Agrosciences Llc High oleic imidazolinone resistant sunflower
UA108733C2 (uk) 2006-12-12 2015-06-10 Толерантна до гербіциду рослина соняшника

Also Published As

Publication number Publication date
WO2013004280A1 (en) 2013-01-10
AR087014A1 (es) 2014-02-05

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