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AR131904A2 - Isolated mutated nucleotide sequences encoding a modified sunflower oleate desaturase protein, modified protein, methods and uses - Google Patents

Isolated mutated nucleotide sequences encoding a modified sunflower oleate desaturase protein, modified protein, methods and uses

Info

Publication number
AR131904A2
AR131904A2 ARP240100392A ARP240100392A AR131904A2 AR 131904 A2 AR131904 A2 AR 131904A2 AR P240100392 A ARP240100392 A AR P240100392A AR P240100392 A ARP240100392 A AR P240100392A AR 131904 A2 AR131904 A2 AR 131904A2
Authority
AR
Argentina
Prior art keywords
modified
protein
nucleotide sequences
oleate desaturase
methods
Prior art date
Application number
ARP240100392A
Other languages
Spanish (es)
Inventor
Alberto Javier Leon
Andrs Daniel Zambelli
Roberto Juan Reid
Mnica Mariel Morata
Marcos Kaspar
Enrique Martinez-Force
Rafael Garces
Joaqun J Salas
Monica Venegas-Caleron
Original Assignee
Advanta Semillas S A I C
Consejo Superior Investigacion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanta Semillas S A I C, Consejo Superior Investigacion filed Critical Advanta Semillas S A I C
Publication of AR131904A2 publication Critical patent/AR131904A2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1464—Helianthus annuus [sunflower]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19006—DELTA12-fatty-acid desaturase (1.14.19.6), i.e. oleoyl-CoA DELTA12 desaturase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención se relaciona con secuencias de nucleótidos aisladas que comprenden una mutación puntual y donde las secuencias codifican una proteína oleato desaturasa modificada de girasol. La proteína oleato desaturasa de girasol modificada comprende al menos una de las siguientes sustituciones de aminoácidos: a) Gly por Asp en la posición 103, b) Ser por Leu en la posición 131; c) Ser por Asn en la posición 135; d) Gly por Glu en la posición 144; e) Gly por Glu en la posición 226; f) Pro por Ser en la posición 253; g) Leu por Phe en la posición 267; h) Pro por Ser en la posición 275; o es una oleato desaturasa truncada.The invention relates to isolated nucleotide sequences comprising a point mutation and wherein the sequences encode a modified sunflower oleate desaturase protein. The modified sunflower oleate desaturase protein comprises at least one of the following amino acid substitutions: a) Gly to Asp at position 103; b) Ser to Leu at position 131; c) Ser to Asn at position 135; d) Gly to Glu at position 144; e) Gly to Glu at position 226; f) Pro to Ser at position 253; g) Leu to Phe at position 267; h) Pro to Ser at position 275; or is a truncated oleate desaturase.

ARP240100392A 2011-07-01 2024-02-21 Isolated mutated nucleotide sequences encoding a modified sunflower oleate desaturase protein, modified protein, methods and uses AR131904A2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2011/061164 WO2013004280A1 (en) 2011-07-01 2011-07-01 Isolated mutated nucleotide sequences that encode a modified oleate destaurase sunflower protein, modified protein, methods and uses

Publications (1)

Publication Number Publication Date
AR131904A2 true AR131904A2 (en) 2025-05-14

Family

ID=44629365

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP120102375A AR087014A1 (en) 2011-07-01 2012-06-29 SEQUENCES OF ISOLATED MUTED NUCLEOTIDS THAT CODIFY A MODIFIED SUNFLOWER OLEATO PROTEIN, MODIFIED PROTEIN, METHODS AND USES
ARP240100392A AR131904A2 (en) 2011-07-01 2024-02-21 Isolated mutated nucleotide sequences encoding a modified sunflower oleate desaturase protein, modified protein, methods and uses

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP120102375A AR087014A1 (en) 2011-07-01 2012-06-29 SEQUENCES OF ISOLATED MUTED NUCLEOTIDS THAT CODIFY A MODIFIED SUNFLOWER OLEATO PROTEIN, MODIFIED PROTEIN, METHODS AND USES

Country Status (2)

Country Link
AR (2) AR087014A1 (en)
WO (1) WO2013004280A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3099178A1 (en) 2019-07-24 2021-01-29 Soltis High oleic acid sunflower and process for obtaining it
EP4611532A1 (en) 2022-11-02 2025-09-10 KWS SAAT SE & Co. KGaA Oleic acid sunflower mutants and methods for detection

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5407594A (en) 1992-11-17 1994-06-08 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6342658B1 (en) * 1995-12-14 2002-01-29 Cargill, Incorporated Fatty acid desaturases and mutant sequences thereof
AR032701A1 (en) 2001-02-16 2003-11-19 Consejo Superior Investigacion A PLANT, SEEDS AND AN OIL WITH INCREASED CONTENT OF SATURATED TRIACILGLYCEROLS AND AN OIL THAT HAS A HIGH CONTENT OF ESTEARIC ACID
US20090158452A1 (en) * 2001-12-04 2009-06-18 Johnson Richard G Transgenic plants with enhanced agronomic traits
EP1591536A1 (en) 2004-04-29 2005-11-02 Institut National de la Recherche Agronomique Method of selecting sunflower genotypes with high oleic acid content in seed oil
WO2006024351A1 (en) 2004-07-30 2006-03-09 Basf Agrochemical Products B.V. Herbicide-resistant sunflower plants, plynucleotides encoding herbicide-resistant acetohydroxy acid synthase large subunit proteins, and methods of use
AU2006294546B2 (en) * 2005-09-28 2012-08-09 Dow Agrosciences Llc High oleic imidazolinone resistant sunflower
UA108733C2 (en) 2006-12-12 2015-06-10 Sunflower herbicide tolerant to herbicide

Also Published As

Publication number Publication date
WO2013004280A1 (en) 2013-01-10
AR087014A1 (en) 2014-02-05

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