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AR106436A1 - Vector de ácido nucleico, método para producir una célula vegetal transgénica con el mismo, promotor empleado en el mismo, célula vegetal transgénica obtenida y polinucleótido aislado de dicho vector - Google Patents

Vector de ácido nucleico, método para producir una célula vegetal transgénica con el mismo, promotor empleado en el mismo, célula vegetal transgénica obtenida y polinucleótido aislado de dicho vector

Info

Publication number
AR106436A1
AR106436A1 ARP160103215A ARP160103215A AR106436A1 AR 106436 A1 AR106436 A1 AR 106436A1 AR P160103215 A ARP160103215 A AR P160103215A AR P160103215 A ARP160103215 A AR P160103215A AR 106436 A1 AR106436 A1 AR 106436A1
Authority
AR
Argentina
Prior art keywords
vector
promoter
plant cell
same
vegetable cell
Prior art date
Application number
ARP160103215A
Other languages
English (en)
Inventor
t beeson William iv
Patrick Connell James
Mann David
Davies John
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR106436A1 publication Critical patent/AR106436A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8229—Meristem-specific, e.g. nodal, apical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8233—Female-specific, e.g. pistil, ovule
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Reivindicación 1: Un vector de ácido nucleico caracterizado porque comprende un promotor operativamente unido a: a) una secuencia polienlazadora; b) un gen KN1 que no es de Zea may; o c) una combinación de a) y b), donde dicho promotor comprende una secuencia de polinucleótidos que posee al menos 90% de identidad de secuencia con la SEC ID Nº 1. Reivindicación 11: Un método para producir una célula vegetal transgénica con el vector de la reivindicación 1, el método caracterizado porque comprende los siguientes pasos: a) transformar una célula vegetal con una casete de expresión génica que comprende un promotor KN1 de Zea mays operativamente unida a al menos una secuencia de polinucleótidos de interés; b) aislar la célula vegetal transformada que comprende la casete de expresión génica; y c) producir una célula vegetal transgénica que comprende el promotor KN1 de Zea mays operativamente unida a al menos una secuencia de polinucleótidos de interés. Reivindicación 20: El promotor KN1 de Zea mays empleado en el método de la reivindicación 11, caracterizado porque el promotor KN1 de Zea mays comprende el polinucleótido de la SEC ID Nº 1. Reivindicación 22: Un método para expresar una secuencia de polinucleótidos de interés en una célula vegetal con el vector de la reivindicación 1, caracterizado porque el método comprende introducir en la célula vegetal una secuencia de polinucleótidos de interés operativamente unida a un promotor de gen KN1 de Zea mays. Reivindicación 30: Una célula vegetal transgénica obtenida mediante el método de la reivindicación 11, caracterizada porque comprende el promotor KN1 de Zea mays. Reivindicación 45: Un polinucleótido aislado caracterizado porque comprende una secuencia de ácidos nucleicos con al menos 90% de identidad de secuencia respecto del polinucleótido de SEC ID Nº 1 comprendida en el vector de la reivindicación 1.
ARP160103215A 2015-10-22 2016-10-21 Vector de ácido nucleico, método para producir una célula vegetal transgénica con el mismo, promotor empleado en el mismo, célula vegetal transgénica obtenida y polinucleótido aislado de dicho vector AR106436A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562244843P 2015-10-22 2015-10-22

Publications (1)

Publication Number Publication Date
AR106436A1 true AR106436A1 (es) 2018-01-17

Family

ID=57346046

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160103215A AR106436A1 (es) 2015-10-22 2016-10-21 Vector de ácido nucleico, método para producir una célula vegetal transgénica con el mismo, promotor empleado en el mismo, célula vegetal transgénica obtenida y polinucleótido aislado de dicho vector

Country Status (8)

Country Link
US (2) US10280429B2 (es)
EP (1) EP3365451B1 (es)
CN (1) CN108473997B (es)
AR (1) AR106436A1 (es)
AU (1) AU2016340893B2 (es)
TW (1) TW201723182A (es)
UY (1) UY36957A (es)
WO (1) WO2017070298A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112063649A (zh) * 2020-09-08 2020-12-11 南京农业大学 一种可见光下裸眼可视的植物遗传转化的无损筛选系统、构建方法及其应用

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Also Published As

Publication number Publication date
EP3365451A1 (en) 2018-08-29
US20190161762A1 (en) 2019-05-30
CA3002151A1 (en) 2017-04-27
AU2016340893A1 (en) 2018-04-26
EP3365451B1 (en) 2020-06-24
WO2017070298A1 (en) 2017-04-27
CN108473997A (zh) 2018-08-31
AU2016340893B2 (en) 2019-06-27
US10731171B2 (en) 2020-08-04
CN108473997B (zh) 2022-04-12
US10280429B2 (en) 2019-05-07
US20170114355A1 (en) 2017-04-27
TW201723182A (zh) 2017-07-01
UY36957A (es) 2017-05-31

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