AR096027A2 - Proteínas insecticidas quiméricas de bacillus thuringiensis - Google Patents
Proteínas insecticidas quiméricas de bacillus thuringiensisInfo
- Publication number
- AR096027A2 AR096027A2 ARP140101586A ARP140101586A AR096027A2 AR 096027 A2 AR096027 A2 AR 096027A2 AR P140101586 A ARP140101586 A AR P140101586A AR P140101586 A ARP140101586 A AR P140101586A AR 096027 A2 AR096027 A2 AR 096027A2
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- acid sequence
- proteins
- lmbp
- cry2ae
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 11
- 102000004169 proteins and genes Human genes 0.000 title abstract 9
- 241000193388 Bacillus thuringiensis Species 0.000 title abstract 2
- 229940097012 bacillus thuringiensis Drugs 0.000 title abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 7
- 230000000749 insecticidal effect Effects 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical group 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002917 insecticide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Compuestos insecticidas derivados de cepas de Bacillus thuringiensis. Se proveen proteínas denominadas Cry2Ae, Cry2Af y Cry2Ag, y variantes de las mismas, así como secuencias de ADN codificadores de estas proteínas o de las variantes de las mismas. Además se proveen huéspedes recombinantes que expresan dichas proteínas, en particular células vegetales y plantas. Reivindicación 1: Un gen quimérico que comprende una secuencia de ácido nucleico bajo el control de un promotor de expresión en plantas, caracterizada porque dicha secuencia de ácido nucleico codifica una proteína insecticida Cry2Ae que comprende una secuencia de aminoácidos seleccionada del grupo formado por : a) la secuencia de aminoácidos de la proteína codificada por el gen cry2Ae depositado en BCCM-LMBP con el Nº Acceso LMBP 4248 o una porción insecticida de la misma, b) la secuencia de aminoácidos de la proteína de la SEQ ID Nº 2, o una porción insecticida de la misma, c) la secuencia de aminoácidos de la proteína de la SEQ ID Nº 2 entre la posición de aminoácido 1 y la posición de aminoácido 625 y d) la secuencia de aminoácidos de una proteína insecticida codificada por un ADN que se hibridiza con la secuencia de codificación cry2Ae depositada en BCCM-LMBP con el Nº Acceso LMBP 4248 bajo condiciones de hibridación severas, donde dicho ADN presenta una identidad de secuencia de por lo menos 93%, en particular por lo menos 98%, preferentemente por lo menos 99%, con la secuencia de codificación de la SEQ ID Nº 1.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75629601A | 2001-01-09 | 2001-01-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR096027A2 true AR096027A2 (es) | 2015-12-02 |
Family
ID=25042854
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020100051A AR032231A1 (es) | 2001-01-09 | 2002-01-09 | Nuevas proteinas insecticidas de bacillus thuringiensis |
| ARP140101586A AR096027A2 (es) | 2001-01-09 | 2014-04-15 | Proteínas insecticidas quiméricas de bacillus thuringiensis |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020100051A AR032231A1 (es) | 2001-01-09 | 2002-01-09 | Nuevas proteinas insecticidas de bacillus thuringiensis |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1352068B1 (es) |
| JP (1) | JP4404549B2 (es) |
| CN (1) | CN1234867C (es) |
| AR (2) | AR032231A1 (es) |
| AT (1) | ATE409231T1 (es) |
| AU (1) | AU2002252974B2 (es) |
| BR (3) | BRPI0206346B1 (es) |
| CA (2) | CA2771117C (es) |
| DE (1) | DE60229037D1 (es) |
| ES (1) | ES2315360T3 (es) |
| MX (1) | MXPA03006130A (es) |
| PT (1) | PT1352068E (es) |
| SI (1) | SI1352068T1 (es) |
| WO (1) | WO2002057664A2 (es) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6593293B1 (en) | 1999-09-15 | 2003-07-15 | Monsanto Technology, Llc | Lepidopteran-active Bacillus thuringiensis δ-endotoxin compositions and methods of use |
| DE60332711D1 (de) | 2002-03-22 | 2010-07-08 | Bayer Bioscience Nv | Neue insektizide proteine von bazillus thuringiensis |
| EP1718751B1 (en) * | 2004-02-25 | 2013-05-01 | Pioneer-Hi-Bred International, Inc. | Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof |
| CN101238215A (zh) * | 2005-04-05 | 2008-08-06 | 先锋高级育种国际公司 | 利用植物病毒密码子偏爱性设计用于在植物中的多肽表达的核酸分子的方法和组合物 |
| BRPI0613111A2 (pt) | 2005-07-08 | 2010-12-21 | Univ Mexico Nacional Autonoma | proteìnas bacterianas com atividade pesticida |
| WO2007107302A2 (en) * | 2006-03-21 | 2007-09-27 | Bayer Bioscience N.V. | Novel genes encoding insecticidal proteins |
| WO2008145406A1 (en) * | 2007-06-01 | 2008-12-04 | Bayer Bioscience N.V. | Novel genes encoding insecticidal proteins |
| AP3195A (en) * | 2007-06-11 | 2015-03-31 | Bayer Cropscience Nv | Insect resistant cotton plants and methods for identifying same |
| US7772465B2 (en) | 2007-06-26 | 2010-08-10 | Pioneer Hi-Bred International, Inc. | Bacillus thuringiensis gene with lepidopteran activity |
| CA2702998C (en) * | 2007-10-16 | 2017-06-06 | Athenix Corp. | Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use |
| CA2727637A1 (en) * | 2008-06-13 | 2009-12-17 | Bayer Bioscience N.V. | Bollworm insect resistance management in transgenic plants |
| WO2010141141A2 (en) * | 2009-03-11 | 2010-12-09 | Athenix Corporation | Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use |
| WO2011075590A1 (en) * | 2009-12-16 | 2011-06-23 | Dow Agrosciences Llc | Insecticidal protein combination comprising cry1ab and cry2aa for controlling european corn borer, and methods for insect resistance management |
| BR112012015125A2 (pt) | 2009-12-23 | 2015-09-01 | Bayer Ip Gmbh | "plantas tolerantes aos herbicidas inibidores de hppd" |
| JP5871814B2 (ja) | 2009-12-23 | 2016-03-01 | バイエル・インテレクチュアル・プロパティ・ゲーエムベーハーＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ Ｇｍｂｈ | Ｈｐｐｄ阻害型除草剤に耐性を有する植物 |
| BR112012015690A2 (pt) | 2009-12-23 | 2015-08-25 | Bayer Intelectual Property Gmbh | Plantas tolerantes a herbicidas inibidores de hppd. |
| ES2668222T3 (es) | 2009-12-23 | 2018-05-17 | Bayer Intellectual Property Gmbh | Plantas tolerantes a herbicidas inhibidores de la HPPD |
| AR079882A1 (es) | 2009-12-23 | 2012-02-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
| EP2669373B1 (en) | 2010-11-10 | 2016-06-01 | Bayer CropScience AG | HPPD variants and methods of use |
| US8648230B2 (en) | 2011-03-18 | 2014-02-11 | Ms Technologies, Llc | Regulatory regions preferentially expressing in non-pollen plant tissue |
| KR20140033354A (ko) | 2011-03-25 | 2014-03-18 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | Hppd 억제 제초제에 대해서 내성인 유전자이식 농작물의 영역에서 원치 않는 식물을 방제하기 위한 n-(테트라졸-4-일)- 또는 n-(트리아졸-3-일)아릴카복사미드 또는 그의 염의 용도 |
| JP5847920B2 (ja) | 2011-03-25 | 2016-01-27 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ | Ｈｐｐｄインヒビター除草剤に対して耐性であるトランスジェニック作物植物の領域において望まぬ植物を制御するためのｎ−（１，２，５−オキサジアゾール−３−イル）ベンズアミドの使用 |
| BR112014022195A2 (pt) | 2012-03-09 | 2020-05-12 | Vestaron Corporation | Produção de peptídeo tóxico, expressão de peptídeo em plantas e combinações de peptídeos ricos em cisteína |
| US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
| CN103571853B (zh) * | 2012-08-08 | 2016-01-20 | 中国科学院亚热带农业生态研究所 | 密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法 |
| US10793872B2 (en) | 2012-09-14 | 2020-10-06 | BASF Agricultural Solutions Seed US LLC | HPPD variants and methods of use |
| CN110172466A (zh) | 2013-03-07 | 2019-08-27 | 巴斯夫农业解决方案种子美国有限责任公司 | 毒素基因及其使用方法 |
| CN103290130B (zh) * | 2013-06-13 | 2015-02-04 | 中华人民共和国上海出入境检验检疫局 | 转基因产品中Cry2Ae基因的检测方法及其试剂盒 |
| US10876130B2 (en) | 2014-03-11 | 2020-12-29 | BASF Agricultural Solutions Seed US LLC | HPPD variants and methods of use |
| CN104286014B (zh) * | 2014-08-27 | 2016-03-23 | 北京大北农科技集团股份有限公司 | 杀虫蛋白的用途 |
| CN105367633B (zh) * | 2014-08-28 | 2018-11-30 | 四川农业大学 | 一种BT蛋白CRY2Ab32、其编码基因及应用 |
| AR105155A1 (es) * | 2015-07-07 | 2017-09-13 | Syngenta Participations Ag | Composiciones y métodos para controlar plagas de plantas |
| KR20180043838A (ko) | 2015-09-11 | 2018-04-30 | 바이엘 크롭사이언스 악티엔게젤샤프트 | Ｈｐｐｄ 변이체 및 사용 방법 |
| AU2017347761C1 (en) | 2016-10-21 | 2024-03-28 | Vestaron Corporation | Cleavable peptides and insecticidal and nematicidal proteins comprising same |
| CN118256514A (zh) | 2016-11-23 | 2024-06-28 | 巴斯夫农业种子解决方案美国有限责任公司 | Axmi669和axmi991毒素基因及其使用方法 |
| KR20190095411A (ko) | 2016-12-22 | 2019-08-14 | 바스프 아그리컬쳐럴 솔루션즈 시드 유에스 엘엘씨 | 선충 해충의 방제를 위한 ｃｒｙ１４의 용도 |
| WO2018136604A1 (en) | 2017-01-18 | 2018-07-26 | Bayer Cropscience Lp | Bp005 toxin gene and methods for its use |
| WO2018136611A1 (en) | 2017-01-18 | 2018-07-26 | Bayer Cropscience Lp | Use of bp005 for the control of plant pathogens |
| CA3055317A1 (en) | 2017-03-07 | 2018-09-13 | BASF Agricultural Solutions Seed US LLC | Hppd variants and methods of use |
| US11279944B2 (en) | 2017-10-24 | 2022-03-22 | BASF Agricultural Solutions Seed US LLC | Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean |
| WO2019083808A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS |
| BR112021021426A2 (pt) * | 2019-04-24 | 2021-12-21 | Dcm Shriram Ltd | Sequência de nucleotídeos sintética com otimização de códon, dna recombinante, construto de dna para expressão de uma proteína inseticida de interesse, vetor plasmidial, célula hospedeira, método para conferir uma resistência a insetos a uma planta, planta transgênica, tecido, semente ou progênie obtida a partir da planta transgênica, amostra biológica derivada dos tecidos ou semente ou progênie, produto primário derivado da planta transgênica, composição, método para controlar a infestação de insetos em uma planta de cultura e fornecer controle de resistência a inseto, e uso da sequência de nucleotídeos sintética com otimização de códon |
| CA3146520A1 (en) * | 2019-07-30 | 2021-02-04 | Dcm Shriram Limited | Synthetic nucleotide sequences encoding insecticidal crystal protein and uses thereof |
| CA3157808A1 (en) | 2019-10-14 | 2021-04-22 | BASF Agricultural Solutions Seed US LLC | Novel insect resistant genes and methods of use |
| AU2020367523A1 (en) | 2019-10-14 | 2022-04-28 | BASF Agricultural Solutions Seed US LLC | Novel insect resistant genes and methods of use |
| CN111334586A (zh) * | 2020-04-02 | 2020-06-26 | 南京农业大学 | 基于as-pcr技术快速检测二化螟鱼尼丁受体基因突变的方法 |
| UY39585A (es) * | 2020-12-23 | 2022-07-29 | Monsanto Technology Llc | Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas |
| CN114717256A (zh) * | 2022-02-19 | 2022-07-08 | 四川农业大学 | 在水稻中高效表达Bt蛋自Cry2Ag1抗草地贪夜蛾的方法 |
| AU2023408205A1 (en) | 2022-12-19 | 2025-06-26 | Basf Agricultural Solutions Us Llc | Methods of identifying and evaluating genes for insect control |
| WO2024137438A2 (en) | 2022-12-19 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Insect toxin genes and methods for their use |
| EP4639167A1 (en) | 2022-12-20 | 2025-10-29 | BASF Agricultural Solutions US LLC | Methods of identifying and evaluating genes for insect control |
| WO2025111030A2 (en) | 2023-07-07 | 2025-05-30 | Basf Agricultural Solutions Us Llc | Use of novel genes for the control of nematode pests |
| WO2025090606A1 (en) | 2023-10-27 | 2025-05-01 | Basf Agricultural Solutions Us Llc | Use of novel genes for the control of nematode pests |
| CN117486985B (zh) * | 2023-10-27 | 2025-07-18 | 安徽农业大学 | 一种杀虫蛋白及其在防治鳞翅目昆虫中的应用 |
| WO2026010930A1 (en) | 2024-07-05 | 2026-01-08 | BASF Agricultural Solutions Seed US LLC | Use of axmi277 for the control of rotylenchulus reniformis nematode pests |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL177516C (nl) | 1978-09-12 | 1985-10-01 | Pidou Bv | Afdichtmanchet. |
| NL8700204A (nl) | 1987-01-28 | 1988-08-16 | Pidou Bv | Afdichtinrichting. |
| JP2001522233A (ja) * | 1997-03-13 | 2001-11-13 | マイコーゲン コーポレーション | バチルス・チューリンギエンシス毒素 |
| US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
| US6593293B1 (en) * | 1999-09-15 | 2003-07-15 | Monsanto Technology, Llc | Lepidopteran-active Bacillus thuringiensis δ-endotoxin compositions and methods of use |
-
2002
- 2002-01-08 JP JP2002557703A patent/JP4404549B2/ja not_active Expired - Lifetime
- 2002-01-08 AT AT02722023T patent/ATE409231T1/de active
- 2002-01-08 EP EP02722023A patent/EP1352068B1/en not_active Expired - Lifetime
- 2002-01-08 CA CA2771117A patent/CA2771117C/en not_active Expired - Lifetime
- 2002-01-08 SI SI200230777T patent/SI1352068T1/sl unknown
- 2002-01-08 BR BRPI0206346-8A patent/BRPI0206346B1/pt unknown
- 2002-01-08 BR BR0206346-8A patent/BR0206346A/pt active IP Right Grant
- 2002-01-08 WO PCT/EP2002/000298 patent/WO2002057664A2/en not_active Ceased
- 2002-01-08 AU AU2002252974A patent/AU2002252974B2/en not_active Expired
- 2002-01-08 CA CA2433817A patent/CA2433817C/en not_active Expired - Lifetime
- 2002-01-08 MX MXPA03006130A patent/MXPA03006130A/es active IP Right Grant
- 2002-01-08 DE DE60229037T patent/DE60229037D1/de not_active Expired - Lifetime
- 2002-01-08 CN CNB028035674A patent/CN1234867C/zh not_active Expired - Lifetime
- 2002-01-08 BR BR122015003517-1A patent/BR122015003517B1/pt active IP Right Grant
- 2002-01-08 ES ES02722023T patent/ES2315360T3/es not_active Expired - Lifetime
- 2002-01-08 PT PT02722023T patent/PT1352068E/pt unknown
- 2002-01-09 AR ARP020100051A patent/AR032231A1/es active IP Right Grant
-
2014
- 2014-04-15 AR ARP140101586A patent/AR096027A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT1352068E (pt) | 2009-01-06 |
| BR122015003517B1 (pt) | 2021-02-02 |
| SI1352068T1 (sl) | 2009-02-28 |
| CA2433817C (en) | 2012-05-22 |
| JP2004522437A (ja) | 2004-07-29 |
| CA2771117A1 (en) | 2002-07-25 |
| CN1234867C (zh) | 2006-01-04 |
| CA2771117C (en) | 2015-03-17 |
| BR0206346A (pt) | 2003-10-28 |
| AR032231A1 (es) | 2003-10-29 |
| ATE409231T1 (de) | 2008-10-15 |
| WO2002057664A2 (en) | 2002-07-25 |
| EP1352068B1 (en) | 2008-09-24 |
| ES2315360T3 (es) | 2009-04-01 |
| DE60229037D1 (de) | 2008-11-06 |
| CA2433817A1 (en) | 2002-07-25 |
| EP1352068A2 (en) | 2003-10-15 |
| CN1484702A (zh) | 2004-03-24 |
| WO2002057664A3 (en) | 2003-04-17 |
| JP4404549B2 (ja) | 2010-01-27 |
| MXPA03006130A (es) | 2005-02-14 |
| AU2002252974B2 (en) | 2006-12-14 |
| BRPI0206346B1 (pt) | 2018-04-03 |
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| FB | Suspension of granting procedure |