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AR038725A1 - Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacion - Google Patents

Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacion

Info

Publication number
AR038725A1
AR038725A1 ARP030100713A ARP030100713A AR038725A1 AR 038725 A1 AR038725 A1 AR 038725A1 AR P030100713 A ARP030100713 A AR P030100713A AR P030100713 A ARP030100713 A AR P030100713A AR 038725 A1 AR038725 A1 AR 038725A1
Authority
AR
Argentina
Prior art keywords
seq
nucleotide sequence
toxin
vip3
procedures
Prior art date
Application number
ARP030100713A
Other languages
English (en)
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27805151&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR038725(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of AR038725A1 publication Critical patent/AR038725A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Agronomy & Crop Science (AREA)
  • Physics & Mathematics (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se revelan novedosas toxinas Vip3 que son sumamente activas contra un amplio espectro de insectos plaga lepidópteros. El ADN que codifuca la toxina Vip3 puede ser utilizado para transformar diversos organismos procariotas y eucariotas para expresar la toxina Vip3. Dichos organismos recombinantes pueden ser utilizados para controlar los insectos lepidópteros en diversos entornos. Reivindicación (1): Una molécula aislada de ácidos nucléicos, que comprende una secuencia de nucleótidos que codifica una toxina que es activa contra los insectos, en la cual dicha secuencia de nucleótidos: (a) - tiene un complemento que se hibrida con los nucleótidos 1981-2367 de la SEQ ID NO: 1 en dodecilsulfato de sodio (SDS) al 7%, NaPO4 0,5 M, EDTA 1 mM a 50 °C, con un lavado en 0,1 XSSC, SDS al 0,1%, a 65 °C; o (b) - es isocodificante con la secuencia de nucleótidos de (a); o (c) - tiene una identidad de secuencias con la SEQ ID NO: 1, de por lo menos el 93%; o (d) --codifica una secuencia de aminoácidos que tiene una identidad de secuencias con la SEQ ID NO: 2, de por lo menos el 91%.
ARP030100713A 2002-03-06 2003-03-04 Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacion AR038725A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36225002P 2002-03-06 2002-03-06

Publications (1)

Publication Number Publication Date
AR038725A1 true AR038725A1 (es) 2005-01-26

Family

ID=27805151

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP030100713A AR038725A1 (es) 2002-03-06 2003-03-04 Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacion
ARP090102302A AR072217A2 (es) 2002-03-06 2009-06-23 Toxina hibrida procedente de bacillusthuringiensis, metodo para controlar insectos, y molecula de acido nucleico

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP090102302A AR072217A2 (es) 2002-03-06 2009-06-23 Toxina hibrida procedente de bacillusthuringiensis, metodo para controlar insectos, y molecula de acido nucleico

Country Status (13)

Country Link
US (3) US7378493B2 (es)
EP (1) EP1499176B1 (es)
JP (1) JP2006500908A (es)
CN (1) CN100473724C (es)
AR (2) AR038725A1 (es)
AT (1) ATE465628T1 (es)
AU (2) AU2003219779B2 (es)
BR (1) BRPI0308104A2 (es)
CA (1) CA2477975C (es)
DE (1) DE60332337D1 (es)
ES (1) ES2344691T3 (es)
WO (1) WO2003075655A2 (es)
ZA (1) ZA200406879B (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0308104A2 (pt) * 2002-03-06 2016-06-28 Syngenta Participations Ag novas toxinas vip 3 e métodos de uso
CA2479769C (en) * 2002-03-22 2016-01-26 Bayer Bioscience N.V. Novel bacillus thuringiensis insecticidal proteins
CN100497378C (zh) * 2002-03-22 2009-06-10 拜尔生物科学公司 新的苏云金芽孢杆菌杀昆虫蛋白质
GB0225129D0 (en) * 2002-10-29 2002-12-11 Syngenta Participations Ag Improvements in or relating to organic compounds
AU2004295386A1 (en) 2003-12-01 2005-06-16 Syngenta Participations Ag Insect resistant cotton plants and methods of detecting the same
WO2005054480A2 (en) * 2003-12-01 2005-06-16 Syngenta Participations Ag Insect resistant cotton plants and methods of detecting the same
UA94893C2 (ru) * 2004-03-25 2011-06-25 Сингента Партисипейшнс Аг Трансгенное растение кукурузы mir604
US8140581B2 (en) * 2008-05-15 2012-03-20 Microsoft Corporation Configurable view on data models
WO2010003065A2 (en) 2008-07-02 2010-01-07 Athenix Corporation Axmi-115, axmi-113, axmi-005, axmi-163 and axmi-184: insecticidal proteins and methods for their use
BRPI1008674A2 (pt) 2009-02-19 2015-08-25 Pioneer Hi Bred Int Métodos de redução do desenvolvimento de pragas resistentes.
RS59239B1 (sr) 2011-04-05 2019-10-31 BASF Agricultural Solutions Seed US LLC Varijanta axmi115 insekticidnog gena i postupci za njenu upotrebu
WO2013004668A1 (en) 2011-07-05 2013-01-10 Syngenta Participations Ag Root preferred promoters derived from rice and methods of use
US9861105B2 (en) 2011-07-28 2018-01-09 Syngenta Participations Ag Methods and compositions for controlling nematode pests
BR112014020164B1 (pt) 2012-02-16 2022-09-13 Syngenta Participations Ag Polipeptídeo pesticida vip3 manipulado, molécula de ácido nucleico, vetor e composição
US9790258B2 (en) * 2012-03-08 2017-10-17 Athenix Corp. AXMI335 toxin gene and methods for its use
HUE039836T2 (hu) * 2013-02-13 2019-02-28 Athenix Corp AXMI184 alkalmazása gyökérféreg rovarok kontrollálására
CN103421099A (zh) * 2013-08-15 2013-12-04 浙江大学 苏云金芽孢杆菌营养期杀虫蛋白Vip3AfAa及其编码基因和应用
AR097995A1 (es) 2013-10-14 2016-04-27 Syngenta Participations Ag Método para sembrar filas de cultivos
CN104651377A (zh) 2013-11-25 2015-05-27 浙江大学 新型的杀虫蛋白
CA2950947A1 (en) * 2014-06-20 2015-12-23 Dow Agrosciences Llc Vegetative insecticidal proteins useful for control of insect pests
WO2016033265A1 (en) * 2014-08-29 2016-03-03 Syngenta Participations Ag Modified vip3 polypeptides
CN104845983B (zh) * 2015-06-05 2018-08-17 东北农业大学 苏云金芽孢杆菌vip3A-6基因、表达蛋白及其应用
CN104845984B (zh) * 2015-06-05 2018-08-17 东北农业大学 苏云金芽胞杆菌表达蛋白对甜菜夜蛾有高毒力的vip3A-1基因
EA037469B1 (ru) * 2015-08-27 2021-03-31 Монсанто Текнолоджи Ллс Новые белки, проявляющие ингибирующую активность по отношению к насекомым
US10743535B2 (en) 2017-08-18 2020-08-18 H&K Solutions Llc Insecticide for flight-capable pests
WO2019067496A1 (en) * 2017-09-26 2019-04-04 Dow Agrosciences Llc CHIMERIC INSECTICIDE PROTEINS
WO2020247465A2 (en) * 2019-06-05 2020-12-10 Syngenta Participations Ag Control of spodoptera

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UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
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Also Published As

Publication number Publication date
US8686232B2 (en) 2014-04-01
AR072217A2 (es) 2010-08-11
ATE465628T1 (de) 2010-05-15
US9347072B2 (en) 2016-05-24
ES2344691T3 (es) 2010-09-03
AU2008216967B2 (en) 2012-02-09
US7378493B2 (en) 2008-05-27
EP1499176A4 (en) 2006-05-03
CN1646006A (zh) 2005-07-27
WO2003075655A3 (en) 2004-05-27
EP1499176B1 (en) 2010-04-28
US20090328254A1 (en) 2009-12-31
BRPI0308104A2 (pt) 2016-06-28
US20140212394A1 (en) 2014-07-31
DE60332337D1 (de) 2010-06-10
ZA200406879B (en) 2006-12-27
AU2003219779A1 (en) 2003-09-22
JP2006500908A (ja) 2006-01-12
CN100473724C (zh) 2009-04-01
EP1499176A2 (en) 2005-01-26
AU2003219779B2 (en) 2008-06-12
AU2008216967A1 (en) 2008-10-09
WO2003075655A2 (en) 2003-09-18
CA2477975A1 (en) 2003-09-18
CA2477975C (en) 2013-01-29
US20050210545A1 (en) 2005-09-22

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