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AR046944A1 - Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismas - Google Patents

Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismas

Info

Publication number
AR046944A1
AR046944A1 ARP040104700A ARP040104700A AR046944A1 AR 046944 A1 AR046944 A1 AR 046944A1 AR P040104700 A ARP040104700 A AR P040104700A AR P040104700 A ARP040104700 A AR P040104700A AR 046944 A1 AR046944 A1 AR 046944A1
Authority
AR
Argentina
Prior art keywords
seq
insecticidal
bacillus thuringiensis
proteins
nucleotide sequences
Prior art date
Application number
ARP040104700A
Other languages
English (en)
Inventor
Judith Donovan
William Donovan
James Engleman
Thomas Malvar
John Pitkin
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR046944A1 publication Critical patent/AR046944A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Aislamiento y caracterizacion de secuencias de nucleotidos que codifican proteínas insecticidas secretadas hacia el espacio extracelular de Bacillus thuringiensis y cepas relacionadas. Las proteínas se aíslan de sobrenadantes de cultivo de Bacillus thuringiensis y cepas relacionadas y presentan actividad insecticida contra insectos lepidopteros incluyendo el barrenador del maíz europeo (ECB), la oruga capullera de tabaco (TBW) y la pollilla de dorso de diamante (DBM). Se describen proteínas insecticidas codificadas por las secuencias de nucleotidos que se hibridizan bajo condiciones severas con las secuencias de nucleotidos aisladas y caracterizadas. Se describen métodos para elaborar y usar células y plantas transgénicas que comprenden dicha secuencia de nucleotidos. Reivindicacion 1: Un polinucleotido aislado, caracterizado porque codifica una proteína toxina insecticida de Bacillus thuringiensis o un fragmento insecticida de la misma, activa contra una plaga de insectos lepidopteros, donde dicha proteína toxina insecticida comprende una secuencia de polipéptidos que presente al menos un 80% aproximadamente de identidad de secuencia con una secuencia de polipéptidos seleccionada del grupo formado por las SEQ ID Ns 4 (TIC900), SEQ ID Ns 6 (TIC402), SEQ ID Ns 8 (TIC403), SEQ ID Ns 10(TIC404), SEQ ID Ns 30 (TIC434), SEQ ID Ns 12 (TIC961), SEQ ID Ns 14 (TIC962), SEQ ID Ns 16(TIC963), SEQ ID Ns 18 (TIC965) y SEQ ID Ns 20(TIC966), o una variante de toxina insecticida activa contra lepidopteros o una porcion de la misma.
ARP040104700A 2003-12-16 2004-12-16 Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismas AR046944A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US52991703P 2003-12-16 2003-12-16

Publications (1)

Publication Number Publication Date
AR046944A1 true AR046944A1 (es) 2006-01-04

Family

ID=35320635

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP040104700A AR046944A1 (es) 2003-12-16 2004-12-16 Composiciones de genes y proteinas insecticidas secretadas de bacillus thuringiensis y usos de las mismas

Country Status (12)

Country Link
US (3) US20080040827A1 (es)
EP (1) EP1708561B1 (es)
CN (1) CN101133079A (es)
AR (1) AR046944A1 (es)
AT (1) ATE487372T1 (es)
BR (1) BRPI0417735A (es)
CA (1) CA2547933C (es)
DE (1) DE602004030044D1 (es)
ES (1) ES2353603T3 (es)
MX (1) MXPA06007005A (es)
WO (1) WO2005107383A2 (es)
ZA (1) ZA200604678B (es)

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DE602004030044D1 (de) 2003-12-16 2010-12-23 Monsanto Technology Llc Bacillus thuringiensis und deren verwendungen
EP1818405B2 (en) 2004-04-09 2025-03-19 Monsanto Technology, LLC Compositions and methods for control of insect infestations in plants
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WO2009036234A1 (en) * 2007-09-14 2009-03-19 Athenix Corporation Synthetic axmi-004 delta-endotoxin genes and methods for their use
CA2711439A1 (en) * 2008-01-10 2009-07-16 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with coleopteran activity
US20100077507A1 (en) * 2008-09-22 2010-03-25 Pioneer Hi-Bred International, Inc. Novel Bacillus Thuringiensis Gene with Lepidopteran Activity
UY38535A (es) 2008-12-23 2020-11-30 Athenix Corp Gen de delta-endotoxinas axmi-004 y métodos de uso del mismo
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WO2011084324A2 (en) * 2009-12-21 2011-07-14 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with lepidopteran activity
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US9238678B2 (en) * 2011-04-07 2016-01-19 Monsanto Technology Llc Insect inhibitory toxin family active against hemipteran and/or lepidopteran insects
BR112014022195A2 (pt) 2012-03-09 2020-05-12 Vestaron Corporation Produção de peptídeo tóxico, expressão de peptídeo em plantas e combinações de peptídeos ricos em cisteína
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
NZ726117A (en) 2014-06-20 2017-10-27 Dow Agrosciences Llc Vegetative insecticidal proteins useful for control of insect pests
EP3207143B1 (en) * 2014-10-16 2023-11-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
HRP20211858T1 (hr) * 2015-07-30 2022-03-04 Monsanto Technology, Llc Novi proteini za inhibiciju insekata
US10055521B2 (en) 2016-04-15 2018-08-21 International Business Machines Corporation Fly-height interaction simulation
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AU2017347761C1 (en) 2016-10-21 2024-03-28 Vestaron Corporation Cleavable peptides and insecticidal and nematicidal proteins comprising same
CN109055251B (zh) * 2017-10-31 2021-08-10 上海大学 一种芽孢杆菌及其应用
CN111909950B (zh) * 2019-05-09 2022-09-23 中国科学院分子植物科学卓越创新中心 用于提高刺糖孢菌中多杀菌素产量的基因及其应用
US11767319B2 (en) 2020-07-15 2023-09-26 Third Harmonic Bio, Inc. Crystalline forms of a selective c-kit kinase inhibitor
EP4247368A4 (en) 2020-11-19 2024-10-23 Third Harmonic Bio, Inc. PHARMACEUTICAL COMPOSITIONS OF A SELECTIVE C-KIT KINASE INHIBITOR AND METHODS FOR THE PREPARATION AND USE THEREOF
CN112480220B (zh) * 2020-12-02 2021-10-12 东北农业大学 苏云金芽胞杆菌Sip1Aa蛋白随机重组突变蛋白
CN112608866B (zh) * 2020-12-22 2022-12-06 四川大学 巨大芽孢杆菌及其活化方法、泡菜制作方法及泡菜
CN114431179A (zh) * 2022-02-25 2022-05-06 生态环境部南京环境科学研究所 一种Bt杀虫蛋白对大型蚤安全性影响的评价方法

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Also Published As

Publication number Publication date
EP1708561A2 (en) 2006-10-11
CA2547933C (en) 2014-08-12
CA2547933A1 (en) 2005-11-17
ZA200604678B (en) 2008-06-25
US20130212748A1 (en) 2013-08-15
ES2353603T3 (es) 2011-03-03
DE602004030044D1 (de) 2010-12-23
BRPI0417735A (pt) 2007-04-03
US8569583B2 (en) 2013-10-29
WO2005107383A3 (en) 2007-04-12
WO2005107383A8 (en) 2007-05-24
US20080040827A1 (en) 2008-02-14
EP1708561B1 (en) 2010-11-10
MXPA06007005A (es) 2008-02-14
EP1708561A4 (en) 2008-03-05
WO2005107383A2 (en) 2005-11-17
ATE487372T1 (de) 2010-11-15
CN101133079A (zh) 2008-02-27
US20110283417A1 (en) 2011-11-17

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