AR006937A1 - Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, y - Google Patents
Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, yInfo
- Publication number
- AR006937A1 AR006937A1 ARP970101812A ARP970101812A AR006937A1 AR 006937 A1 AR006937 A1 AR 006937A1 AR P970101812 A ARP970101812 A AR P970101812A AR P970101812 A ARP970101812 A AR P970101812A AR 006937 A1 AR006937 A1 AR 006937A1
- Authority
- AR
- Argentina
- Prior art keywords
- vector
- trehalose
- cells
- organs
- tissues
- Prior art date
Links
- 239000013598 vector Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 230000004048 modification Effects 0.000 title abstract 3
- 238000012986 modification Methods 0.000 title abstract 3
- 210000000056 organ Anatomy 0.000 title abstract 3
- 238000010367 cloning Methods 0.000 title 1
- LABSPYBHMPDTEL-JGZVXCDNSA-N trehalose-6-phosphate Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@@H]1O[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](COP(O)(O)=O)O1 LABSPYBHMPDTEL-JGZVXCDNSA-N 0.000 abstract 5
- 230000006698 induction Effects 0.000 abstract 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 abstract 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 abstract 2
- 101710197404 Trehalose-phosphate phosphatase Proteins 0.000 abstract 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 230000033228 biological regulation Effects 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 230000019522 cellular metabolic process Effects 0.000 abstract 1
- 239000013599 cloning vector Substances 0.000 abstract 1
- 230000004907 flux Effects 0.000 abstract 1
- 230000034659 glycolysis Effects 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 230000037041 intracellular level Effects 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- -1 saccharide trehalose-6-phosphate Chemical class 0.000 abstract 1
- 108010020589 trehalose-6-phosphate synthase Proteins 0.000 abstract 1
- 108020003272 trehalose-phosphatase Proteins 0.000 abstract 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8269—Photosynthesis
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01028—Alpha,alpha-trehalase (3.2.1.28)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
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- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Cereal-Derived Products (AREA)
Abstract
Regulación de flujo de carbono en el metabolismo de las células. Se ha hallado que la inducción de un cambio en la disponibilidad intracelular delsacárico trehalosa-6-fosfato (T-6-P) induce modificaciones en el desarrollo y/o composición delascélulas, tejidos y organos in vivo. Estos cambios puedenser inducidos por la inducción o inhibición de la enzima trehalosa fosfato sintasa (TPS) que es capaz de formar T-6-P y trehalosa fosfato fosfatasa (TPP)que degrada T-6-P a trehalosa.El flujo de carbono a través de la glucólisis sera estimulado por una disminución del nivel intracelular de T-6-P. Asíse refiere a un método de modificación del desarrollo y/o composición de células, tejidos u órganos in vivo por medio dela inducción de unamodificación en la disponibilidad metabólica de trehalosa-6-fosfato. También se refiere a un vector de clonación utilizado en dicho método, a una plantao parte de planta transformada con dicho vector, a un organismo huésped quecomprende a dicho vector, a una célula transformada con dicho organismo, ya polinucleótidos que codifican trehalosa-6-fosfato sintasa y trehalosa-6- fosfato fosfatasa.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96201225 | 1996-05-03 | ||
| EP96202128 | 1996-07-26 | ||
| EP96202395 | 1996-08-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR006937A1 true AR006937A1 (es) | 1999-09-29 |
Family
ID=27237548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP970101812A AR006937A1 (es) | 1996-05-03 | 1997-05-02 | Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, y |
Country Status (24)
| Country | Link |
|---|---|
| US (4) | US6833490B1 (es) |
| EP (1) | EP0901527B1 (es) |
| JP (1) | JP2000510691A (es) |
| KR (1) | KR20000010758A (es) |
| CN (1) | CN1267558C (es) |
| AR (1) | AR006937A1 (es) |
| AT (1) | ATE301718T1 (es) |
| AU (2) | AU718379B2 (es) |
| BR (1) | BR9710959A (es) |
| CA (1) | CA2253348C (es) |
| CZ (1) | CZ345098A3 (es) |
| DE (1) | DE69733945T2 (es) |
| DK (1) | DK0901527T3 (es) |
| ES (1) | ES2243997T3 (es) |
| IL (1) | IL126781A0 (es) |
| IN (1) | IN1997CH00924A (es) |
| MA (1) | MA24248A1 (es) |
| MX (1) | MX9809128A (es) |
| NO (1) | NO985097L (es) |
| NZ (1) | NZ332677A (es) |
| PL (1) | PL329913A1 (es) |
| SK (1) | SK148498A3 (es) |
| TR (4) | TR199902037T2 (es) |
| WO (1) | WO1997042326A2 (es) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5854067A (en) * | 1996-01-19 | 1998-12-29 | Board Of Regents, The University Of Texas System | Hexokinase inhibitors |
| US5891717A (en) * | 1996-01-19 | 1999-04-06 | Betagene, Inc. | Methods and compositions for inhibiting hexokinase |
| IN1997CH00924A (en) * | 1996-05-03 | 2005-03-04 | Syngenta Mogen Bv | Regulating metabolism by modifying the level of trehalose-6-phosphate |
| MX205414B (es) * | 1996-05-08 | 2001-12-07 | Univ Mexico Nacional Autonoma | Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla. |
| IL132498A0 (en) * | 1997-05-02 | 2001-03-19 | Mogen Int | Regulating metabolism by modifying the level of trehalose-6-phosphate by inhibiting endogenous trehalase levels |
| ZA989782B (en) | 1997-10-30 | 1999-05-04 | Mogen Int | Pre-and postharvest inhibition of remobilisation of storage compounds |
| WO1999023225A1 (en) * | 1997-10-30 | 1999-05-14 | Mogen International N.V. | Novel high-fermenting microorganisms |
| ZA989886B (en) * | 1997-10-30 | 1999-04-30 | Mogen Int | Nuclear male sterile plants method of producing same and methods to restore fertility |
| AU2412499A (en) * | 1997-10-30 | 1999-05-31 | Mogen International N.V. | Novel high-fermenting microorganisms |
| DE19832334A1 (de) * | 1998-07-17 | 2000-01-20 | Norika Nordring Kartoffelzucht | Glucosylglycerolphosphatsynthasegene zur Erzeugung der niedermolekularen Schutzsubstanz Glucosylglycerol und ihre Verwendung |
| EP1002867A1 (en) * | 1998-10-15 | 2000-05-24 | K.U. Leuven Research & Development | Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment |
| US7612255B2 (en) * | 1999-02-03 | 2009-11-03 | Jonathan Gressel | Transgenic plants for mitigating introgression of genetically engineered genetic traits |
| DE60039079D1 (de) | 1999-09-01 | 2008-07-10 | Monsanto Technology Llc | Pflanzen-Promotersequenzen aus Mais, die selektiv die Genexpression kontrollieren |
| CA2401504A1 (en) * | 2000-03-01 | 2001-09-07 | Research & Development Institute, Inc. | Transgenic plants with increased seed yield, biomass and harvest index |
| GB0100105D0 (en) * | 2001-01-04 | 2001-02-14 | Leuven K U Res & Dev | The use of plant TPS (Trehalose-6-phosphate syntase) as a selectable marker for plant transformation |
| UA84669C2 (ru) | 2001-03-14 | 2008-11-25 | Юніверсити Оф Флорида Рисерч Фаундейшн, Инк. | Теплостабильные мутанты ферментов биосинтеза крахмала |
| US8022272B2 (en) | 2001-07-13 | 2011-09-20 | Sungene Gmbh & Co. Kgaa | Expression cassettes for transgenic expression of nucleic acids |
| AR037626A1 (es) | 2001-12-03 | 2004-11-17 | Univ Florida | Variantes de adp-glucosa pirofosforilasa que afectan la sensibilidad al fosfato y su empleo en plantas transgenicas para la regulacion de la biosintesis de almidon |
| KR100440725B1 (ko) * | 2002-06-20 | 2004-07-15 | 주식회사 그린진 바이오텍 | 비생물성 스트레스에 대한 단자엽 식물의 내성을증가시키는 방법 |
| US20040187175A1 (en) * | 2002-11-06 | 2004-09-23 | Wu Ray J. | TPS plant gene constructs and transformants |
| ES2305849T3 (es) * | 2003-07-29 | 2008-11-01 | Ajinomoto Co., Inc. | Procedimiento para la produccion de l-lisina o l-treonina utilizando la bacteria escherichia que presenta actividad de la enzima malica atenuada. |
| US20070184067A1 (en) * | 2003-08-08 | 2007-08-09 | Dusan Miljkovic | Alimentary compositions and methods for metabolic modulation |
| EP1742527B1 (en) * | 2004-04-20 | 2013-02-20 | Syngenta Participations AG | Regulatory sequences for expressing gene products in plant reproductive tissue |
| EP2163632A1 (en) | 2004-10-05 | 2010-03-17 | SunGene GmbH | Constitutive expression cassettes for regulation of plant expression |
| EP1655364A3 (en) | 2004-11-05 | 2006-08-02 | BASF Plant Science GmbH | Expression cassettes for seed-preferential expression in plants |
| EP1662000B1 (en) | 2004-11-25 | 2011-03-30 | SunGene GmbH | Expression cassettes for guard cell-preferential expression in plants |
| EP1827081A4 (en) * | 2004-12-03 | 2009-04-01 | Syngenta Participations Ag | STRESS TOLERANCE IN PLANTS THROUGH SELECTIVE INHIBITION OF TREHALOSE-6-PHOSPHATE PHOSPHATASE |
| EP1666599A3 (en) | 2004-12-04 | 2006-07-12 | SunGene GmbH | Expression cassettes for mesophyll- and/or epidermis-preferential expression in plants |
| EP2163634A1 (en) | 2004-12-08 | 2010-03-17 | SunGene GmbH | Expression cassettes for vascular tissue-preferential expression in plants |
| CN100355883C (zh) * | 2004-12-10 | 2007-12-19 | 中国科学院上海生命科学研究院 | 水稻抗逆相关基因-海藻糖-6-磷酸磷酸化酶基因及其应用 |
| EP1669456A3 (en) | 2004-12-11 | 2006-07-12 | SunGene GmbH | Expression cassettes for meristem-preferential expression in plants |
| WO2006074437A2 (en) * | 2005-01-07 | 2006-07-13 | E.I. Dupont De Nemours And Company | Nucleotide sequences encoding ramosa3 and sister of ramosa3 and methods of use for same |
| CN101155922A (zh) | 2005-02-09 | 2008-04-02 | 巴斯福植物科学有限公司 | 在单子叶植物中调控表达的表达盒 |
| CN101128591B (zh) | 2005-02-26 | 2013-02-13 | 巴斯福植物科学有限公司 | 用于植物中种子偏好性表达的表达盒 |
| WO2006094976A2 (en) | 2005-03-08 | 2006-09-14 | Basf Plant Science Gmbh | Expression enhancing intron sequences |
| ATE552343T1 (de) | 2005-04-19 | 2012-04-15 | Basf Plant Science Gmbh | Endosperm-spezifische expression und/oder expression in keimenden embryos monokotyledoner pflanzen |
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- 1997-05-02 NZ NZ332677A patent/NZ332677A/en unknown
- 1997-05-02 US US09/171,937 patent/US6833490B1/en not_active Expired - Lifetime
- 1997-05-02 CN CNB971954496A patent/CN1267558C/zh not_active Expired - Lifetime
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- 1997-05-02 SK SK1484-98A patent/SK148498A3/sk unknown
- 1997-05-02 EP EP97923086A patent/EP0901527B1/en not_active Expired - Lifetime
- 1997-05-02 TR TR1999/02037T patent/TR199902037T2/xx unknown
- 1997-05-02 WO PCT/EP1997/002497 patent/WO1997042326A2/en not_active Ceased
- 1997-05-02 ES ES97923086T patent/ES2243997T3/es not_active Expired - Lifetime
- 1997-05-02 DK DK97923086T patent/DK0901527T3/da active
- 1997-05-02 KR KR1019980708875A patent/KR20000010758A/ko not_active Withdrawn
- 1997-05-02 TR TR1998/02220T patent/TR199802220T2/xx unknown
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- 1997-05-02 DE DE69733945T patent/DE69733945T2/de not_active Expired - Lifetime
- 1997-05-02 JP JP09539558A patent/JP2000510691A/ja active Pending
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