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MX2022005304A - Mutaciones de la enzima crispr que reducen los efectos fuera del blanco. - Google Patents

Mutaciones de la enzima crispr que reducen los efectos fuera del blanco.

Info

Publication number
MX2022005304A
MX2022005304A MX2022005304A MX2022005304A MX2022005304A MX 2022005304 A MX2022005304 A MX 2022005304A MX 2022005304 A MX2022005304 A MX 2022005304A MX 2022005304 A MX2022005304 A MX 2022005304A MX 2022005304 A MX2022005304 A MX 2022005304A
Authority
MX
Mexico
Prior art keywords
crispr
crispr enzyme
target effects
enzyme
cas9
Prior art date
Application number
MX2022005304A
Other languages
English (en)
Inventor
Bernd Zetsche
Ian Slaymaker
Linyi Gao
Feng Zhang
Original Assignee
Broad Inst Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc filed Critical Broad Inst Inc
Publication of MX2022005304A publication Critical patent/MX2022005304A/es

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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • G—PHYSICS
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    • G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Business, Economics & Management (AREA)
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  • Computational Mathematics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Medical Informatics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

Se divulgan y reivindican mutaciones o modificaciones de la enzima CRISPR, por ejemplo, una enzima Cas, tal como Cas9, que producen una mejora, por ejemplo, una reducción de los efectos fuera del blanco de una enzima CRISPR-Cas o CRISPR o un sistema o complejo CRISPR-Cas9 que contiene o incluye dicha enzima Cas o enzima CRISPR o Cas9 mutada o modificada. También se divulgan y reivindican métodos para producir y usar y usos de dicha Cas o enzima CRISPR o Cas9 mutada o modificada y sistemas o complejos que la contienen y productos obtenidos a partir de dichos métodos y usos.
MX2022005304A 2015-06-18 2017-12-14 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco. MX2022005304A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562181453P 2015-06-18 2015-06-18
US201562207312P 2015-08-19 2015-08-19
US201562237360P 2015-10-05 2015-10-05
US201562255256P 2015-11-13 2015-11-13
US201562269876P 2015-12-18 2015-12-18

Publications (1)

Publication Number Publication Date
MX2022005304A true MX2022005304A (es) 2022-08-02

Family

ID=56345216

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017016289A MX392008B (es) 2015-06-18 2016-06-17 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco
MX2022005304A MX2022005304A (es) 2015-06-18 2017-12-14 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2017016289A MX392008B (es) 2015-06-18 2016-06-17 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco

Country Status (14)

Country Link
US (4) US10876100B2 (es)
EP (2) EP3929287A3 (es)
JP (3) JP7107683B2 (es)
KR (2) KR102575342B1 (es)
CN (2) CN108290933A (es)
AU (2) AU2016280893B2 (es)
CA (1) CA2989830A1 (es)
IL (3) IL293323B2 (es)
MX (2) MX392008B (es)
RU (1) RU2752834C2 (es)
SG (1) SG10201912329YA (es)
TW (1) TWI813532B (es)
WO (1) WO2016205613A1 (es)
ZA (1) ZA201708498B (es)

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