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MX2017010746A - Composicion y metodos para la expresion regulada de un complejo de arn guia/endonucleasa cas. - Google Patents

Composicion y metodos para la expresion regulada de un complejo de arn guia/endonucleasa cas.

Info

Publication number
MX2017010746A
MX2017010746A MX2017010746A MX2017010746A MX2017010746A MX 2017010746 A MX2017010746 A MX 2017010746A MX 2017010746 A MX2017010746 A MX 2017010746A MX 2017010746 A MX2017010746 A MX 2017010746A MX 2017010746 A MX2017010746 A MX 2017010746A
Authority
MX
Mexico
Prior art keywords
methods
guide rna
cas endonuclease
composition
regulated expression
Prior art date
Application number
MX2017010746A
Other languages
English (en)
Inventor
Svitashev Sergei
Mark Cigan Andrew
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2017010746A publication Critical patent/MX2017010746A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Se proporcionan composiciones y métodos para la expresión regulada de un sistema de ARN guía/endonucleasa Cas en una célula vegetal, planta y semilla. También se proporcionan composiciones y métodos para la modificación genómica de una secuencia diana en el genoma de una planta o célula vegetal. En los métodos y las composiciones se emplea un sistema regulado de ARN guía/endonucleasa Cas para proporcionar un sistema eficaz para modificar o alterar sitios diana dentro del genoma de una planta, célula vegetal o semilla.
MX2017010746A 2015-02-25 2016-02-15 Composicion y metodos para la expresion regulada de un complejo de arn guia/endonucleasa cas. MX2017010746A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562120421P 2015-02-25 2015-02-25
PCT/US2016/017937 WO2016137774A1 (en) 2015-02-25 2016-02-15 Composition and methods for regulated expression of a guide rna/cas endonuclease complex

Publications (1)

Publication Number Publication Date
MX2017010746A true MX2017010746A (es) 2017-11-28

Family

ID=55538592

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017010746A MX2017010746A (es) 2015-02-25 2016-02-15 Composicion y metodos para la expresion regulada de un complejo de arn guia/endonucleasa cas.

Country Status (9)

Country Link
US (1) US20180002715A1 (es)
EP (1) EP3262176A1 (es)
CN (1) CN107406858A (es)
AU (1) AU2016223151A1 (es)
BR (1) BR112017018327A2 (es)
CA (1) CA2985991A1 (es)
MX (1) MX2017010746A (es)
WO (1) WO2016137774A1 (es)
ZA (1) ZA201704779B (es)

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Also Published As

Publication number Publication date
BR112017018327A2 (pt) 2018-04-17
AU2016223151A1 (en) 2017-08-03
WO2016137774A1 (en) 2016-09-01
US20180002715A1 (en) 2018-01-04
EP3262176A1 (en) 2018-01-03
ZA201704779B (en) 2019-06-26
CA2985991A1 (en) 2016-09-01
CN107406858A (zh) 2017-11-28

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