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MX2009004043A - Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas. - Google Patents

Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.

Info

Publication number
MX2009004043A
MX2009004043A MX2009004043A MX2009004043A MX2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A
Authority
MX
Mexico
Prior art keywords
unnatural amino
proteins
amino acids
host cells
mammalian cells
Prior art date
Application number
MX2009004043A
Other languages
English (en)
Inventor
Peter G Schultz
Wenshe Liu
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2009004043A publication Critical patent/MX2009004043A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invención es concerniente con pares ortogonales de tARN t aminoacil-tARN sintetasas que pueden incorporar aminoácidos no naturales a proteínas en células huésped mamíferas, por ejemplo, células huésped de primate y células huésped de roedor. La invención proporciona, por ejemplo pero no limitado a, sistemas de traducción que incluyen células huésped (por ejemplo, células de primate o células de roedor), aminoacil-tARN sintetasas ortogonales derivadas de sintetasas eubacterianas, tARN ortogonales y el aminoácido no natural. La invención también es concerniente con métodos para producir proteínas de interés que comprenden por lo menos un aminoácido no natural en sistemas de célula huésped mamíferos.
MX2009004043A 2006-10-18 2007-10-17 Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas. MX2009004043A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85300806P 2006-10-18 2006-10-18
US92345807P 2007-04-12 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Publications (1)

Publication Number Publication Date
MX2009004043A true MX2009004043A (es) 2009-04-27

Family

ID=39512252

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009004043A MX2009004043A (es) 2006-10-18 2007-10-17 Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.

Country Status (12)

Country Link
US (2) US20080166766A1 (es)
EP (1) EP2069481A4 (es)
JP (1) JP2010506591A (es)
KR (1) KR20090094812A (es)
CN (1) CN101535338A (es)
AU (1) AU2007333010A1 (es)
BR (1) BRPI0718389A2 (es)
CA (1) CA2665678A1 (es)
IL (1) IL197917A0 (es)
MX (1) MX2009004043A (es)
RU (1) RU2009112104A (es)
WO (1) WO2008073184A2 (es)

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US6927042B2 (en) * 2002-10-16 2005-08-09 The Scripps Research Institute Glycoprotein synthesis
KR101171397B1 (ko) 2003-04-17 2012-08-07 더 스크립스 리서치 인스티튜트 진핵 유전자 코드의 확장
ATE471385T1 (de) 2003-07-07 2010-07-15 Scripps Research Inst Zusammensetzungen aus orthogonalen lysyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendung
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
US9657289B2 (en) 2010-12-03 2017-05-23 The University Of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
EP2898085B1 (en) * 2012-09-24 2019-01-23 MedImmune Limited Cell lines
WO2014140347A2 (en) * 2013-03-15 2014-09-18 Allozyne, Inc Novel nucleic acid molecules
CN109295025B (zh) * 2014-09-03 2021-04-02 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN107012121B (zh) * 2016-01-27 2021-01-26 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
MX2019009839A (es) 2017-02-17 2019-10-22 Rubius Therapeutics Inc Celulas eritroides funcionalizadas.
MY202215A (en) 2017-06-02 2024-04-17 Ambrx Inc Methods and compositions for promoting non-natural amino acid-containing protein production
AU2018281334A1 (en) 2017-06-06 2019-12-19 Kindred Biosciences, Inc. Erythropoietin and analogs for veterinary use
WO2019204636A1 (en) 2018-04-19 2019-10-24 Kindred Biosciences, Inc. Variant asparaginase polypeptides for medical use
JP2021536263A (ja) * 2018-09-07 2021-12-27 メディミューン，エルエルシー 細胞選択の方法
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
US20230279378A1 (en) * 2019-10-15 2023-09-07 Trustees Of Boston College Chimeric thermostable aminoacyl-trna synthetase for enhanced unnatural amino acid incorporation
EP4074829A4 (en) * 2019-12-12 2024-01-10 Chugai Seiyaku Kabushiki Kaisha METHOD FOR PRODUCING A PEPTIDE WITH NON-NATURAL AMINO ACID
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697A (zh) * 2024-12-20 2025-03-21 天津大学 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1456360T3 (en) 2001-04-19 2015-08-31 Scripps Research Inst Methods and Composition for Preparation of Orthogonal TRNA-Aminoacyl-TRNA Synthetase Pairs
PT1578949E (pt) 2002-10-16 2009-07-14 Scripps Research Inst Incorporação específica do local de cetoaminoácidos em proteínas
US6927042B2 (en) 2002-10-16 2005-08-09 The Scripps Research Institute Glycoprotein synthesis
WO2004039989A1 (ja) * 2002-10-31 2004-05-13 Riken 非天然型アミノ酸組み込みタンパク質の発現方法
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US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
US7527943B2 (en) 2003-07-07 2009-05-05 The Scripps Research Institute Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
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AU2004282577B2 (en) 2003-10-14 2009-10-29 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
BRPI0417801A (pt) 2003-12-18 2007-04-10 Scripps Research Inst incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas
JP2007519422A (ja) * 2004-02-02 2007-07-19 アンブレツクス・インコーポレイテツド 修飾されたヒト四螺旋バンドルポリペプチド及びそれらの使用
WO2005116237A2 (en) 2004-05-25 2005-12-08 The Scripps Research Institute Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
US20090181858A1 (en) * 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
PT1794323E (pt) 2004-09-21 2014-10-30 Scripps Research Inst Incorporação in vivo de aminoácidos alquinilo em proteínas em eubactérias
US20080171317A1 (en) 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
WO2006045116A2 (en) 2004-10-20 2006-04-27 The Scripps Research Institute In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
JP5135504B2 (ja) 2004-10-27 2013-02-06 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸のインビボ組込み用直交翻訳成分
EP1934343A4 (en) 2005-10-12 2009-09-16 Scripps Research Inst SELECTIVE MODIFICATION POST-TRANSLATION OF POLYPEPTIDES EXPRESSED ON THE SURFACE OF PHAGES
CA2638763A1 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
MX2008011669A (es) 2006-03-16 2008-09-22 Scripps Research Inst Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Also Published As

Publication number Publication date
EP2069481A4 (en) 2010-01-06
CN101535338A (zh) 2009-09-16
JP2010506591A (ja) 2010-03-04
EP2069481A2 (en) 2009-06-17
WO2008073184A3 (en) 2008-11-27
BRPI0718389A2 (pt) 2012-04-10
WO2008073184A2 (en) 2008-06-19
CA2665678A1 (en) 2008-06-19
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
RU2009112104A (ru) 2010-11-27
US20100021963A1 (en) 2010-01-28
AU2007333010A1 (en) 2008-06-19
KR20090094812A (ko) 2009-09-08
IL197917A0 (en) 2011-08-01

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