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MX2008011669A - Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina. - Google Patents

Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Info

Publication number
MX2008011669A
MX2008011669A MX2008011669A MX2008011669A MX2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A
Authority
MX
Mexico
Prior art keywords
amino acid
unnatural amino
phenylselenocysteine
proteins
novel
Prior art date
Application number
MX2008011669A
Other languages
English (en)
Inventor
Peter G Schultz
Jiangyun Wang
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2008011669A publication Critical patent/MX2008011669A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención es concerniente con pares ortogonales de tARN y aminoacil-tARN sintetasas que pueden incorporar el aminoácido no natural fenilselenocisteína a proteínas producidas en células huésped eubacterianas tales como E. coli. La invención proporciona, por ejemplo pero no limitado a, nuevas aminoacil-tARN sintetasas ortogonales, polinucleótidos que codifican las nuevas moléculas de sintetasa, métodos para identificar y enmascarar las nuevas sintetasas, métodos para producir proteínas que contienen el aminoácido no natural fenilselenocisteína y sistemas de traducción. La invención proporciona además métodos para producir proteínas modificadas (por ejemplo, proteínas lapidadas) por medio de la modificación apuntada del residuo de fenilselenocisteína en una proteína.
MX2008011669A 2006-03-16 2007-03-13 Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina. MX2008011669A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US78327206P 2006-03-16 2006-03-16
US86145606P 2006-11-28 2006-11-28
PCT/US2007/006382 WO2007109035A2 (en) 2006-03-16 2007-03-13 Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Publications (1)

Publication Number Publication Date
MX2008011669A true MX2008011669A (es) 2008-09-22

Family

ID=38522926

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2008011669A MX2008011669A (es) 2006-03-16 2007-03-13 Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Country Status (12)

Country Link
US (3) US7790847B2 (es)
EP (1) EP1994165B1 (es)
JP (1) JP5249194B2 (es)
KR (1) KR20080113226A (es)
CN (1) CN101405401B (es)
AU (1) AU2007227592B2 (es)
BR (1) BRPI0709603A2 (es)
CA (1) CA2638772A1 (es)
IL (1) IL193722A0 (es)
MX (1) MX2008011669A (es)
RU (1) RU2464315C2 (es)
WO (1) WO2007109035A2 (es)

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US6927042B2 (en) * 2002-10-16 2005-08-09 The Scripps Research Institute Glycoprotein synthesis
KR101171397B1 (ko) 2003-04-17 2012-08-07 더 스크립스 리서치 인스티튜트 진핵 유전자 코드의 확장
ATE471385T1 (de) 2003-07-07 2010-07-15 Scripps Research Inst Zusammensetzungen aus orthogonalen lysyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendung
JP5135504B2 (ja) * 2004-10-27 2013-02-06 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸のインビボ組込み用直交翻訳成分
CA2638763A1 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
MX2008011669A (es) 2006-03-16 2008-09-22 Scripps Research Inst Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.
JP2009538136A (ja) * 2006-05-23 2009-11-05 ザ スクリップス リサーチ インスティテュート 遺伝的にコードされた蛍光クマリンアミノ酸
CN101516901B (zh) 2006-09-21 2014-01-08 斯克利普斯研究院 选择性硫酸化的蛋白质在真细菌中的遗传编程表达
US20080166766A1 (en) 2006-10-18 2008-07-10 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
CN102203257B (zh) 2007-10-25 2014-01-08 斯克利普斯研究院 3-氨基酪氨酸遗传掺入还原酶
EP2220218A4 (en) * 2007-11-02 2010-12-08 Scripps Research Inst GENETICALLY ENCODED BORONATE AMINO ACID
WO2009061369A2 (en) 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
CN105647824A (zh) * 2007-12-11 2016-06-08 斯克利普斯研究院 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达
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US8709772B2 (en) 2008-01-03 2014-04-29 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
US8318172B2 (en) * 2008-02-08 2012-11-27 The Scripps Research Institute Breaking immunological tolerance with a genetically encoded unnatural amino acid
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8637306B2 (en) * 2008-12-10 2014-01-28 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN103215235B (zh) * 2012-01-18 2014-11-05 中国科学院生物物理研究所 3-咪唑基酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
JP6421122B2 (ja) 2012-10-12 2018-11-07 ストロ バイオファーマ， インコーポレイテッド 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化
CN103820410B (zh) * 2012-11-16 2016-03-30 中国科学院生物物理研究所 3-甲硫酪氨酸翻译系统及其应用
CN104004723B (zh) * 2013-02-22 2016-08-17 中国科学院生物物理研究所 3,5-二氟代酪氨酸翻译系统及其应用
CN106047846B (zh) * 2013-03-22 2020-02-04 中国科学院生物物理研究所 8-羟基喹啉丙氨酸翻译系统及其应用
US10286078B2 (en) * 2013-09-13 2019-05-14 The California Institute For Biomedical Research Modified therapeutic agents and compositions thereof
CN106456589B (zh) 2013-12-18 2020-07-07 斯克利普斯研究所 修饰的治疗剂、订合的肽脂质缀合物及其组合物
EP4393549A3 (en) * 2016-05-16 2024-10-09 The Board of Regents of the University of Texas System Compositions for the delivery of trna as nanoparticles and methods of use therewith
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CN114644581B (zh) * 2021-01-04 2024-07-16 北京惠大生物科技有限公司 含芳基硫酚或芳基硒酚经修饰的氨基酸、重组蛋白及其生物合成方法及应用
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CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

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MX2008011669A (es) 2006-03-16 2008-09-22 Scripps Research Inst Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Also Published As

Publication number Publication date
EP1994165A4 (en) 2012-11-07
US8394605B2 (en) 2013-03-12
KR20080113226A (ko) 2008-12-29
CN101405401A (zh) 2009-04-08
AU2007227592B2 (en) 2011-11-24
JP2009529877A (ja) 2009-08-27
US20070238152A1 (en) 2007-10-11
CA2638772A1 (en) 2007-09-27
US8217145B2 (en) 2012-07-10
CN101405401B (zh) 2013-03-27
US20090209000A1 (en) 2009-08-20
WO2007109035A3 (en) 2008-10-16
RU2008135114A (ru) 2010-04-27
EP1994165B1 (en) 2014-11-05
WO2007109035A2 (en) 2007-09-27
US7790847B2 (en) 2010-09-07
EP1994165A2 (en) 2008-11-26
AU2007227592A1 (en) 2007-09-27
IL193722A0 (en) 2011-08-01
JP5249194B2 (ja) 2013-07-31
RU2464315C2 (ru) 2012-10-20
BRPI0709603A2 (pt) 2011-07-19
US20120282668A1 (en) 2012-11-08

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