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MX2007003142A - Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias. - Google Patents

Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias.

Info

Publication number
MX2007003142A
MX2007003142A MX2007003142A MX2007003142A MX2007003142A MX 2007003142 A MX2007003142 A MX 2007003142A MX 2007003142 A MX2007003142 A MX 2007003142A MX 2007003142 A MX2007003142 A MX 2007003142A MX 2007003142 A MX2007003142 A MX 2007003142A
Authority
MX
Mexico
Prior art keywords
amino acids
eubacteria
alkynyl amino
proteins
synthetases
Prior art date
Application number
MX2007003142A
Other languages
English (en)
Inventor
Alexander Deiters
Peter Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2007003142A publication Critical patent/MX2007003142A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La invencion es concerniente con pares ortogonales de tARN y aminoacil-tARN sintetasas que pueden incorporar alquinil aminoacidos tales como para-propargiloxifenilalanina a proteinas producidas en una eubacteria huesped tal como E. coli. La invencion proporciona nuevas sintetasas ortogonales, metodos para identificar y fabricar tales novedosas sintetasas, metodos para producir proteinas que contiene alquinil aminoacidos y sistemas de traduccion celular.
MX2007003142A 2004-09-21 2005-09-20 Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias. MX2007003142A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61222004P 2004-09-21 2004-09-21
US63087604P 2004-11-24 2004-11-24
US63415104P 2004-12-07 2004-12-07
PCT/US2005/033784 WO2006034332A2 (en) 2004-09-21 2005-09-20 In vivo incorporation of alkynyl amino acids into proteins in eubacteria

Publications (1)

Publication Number Publication Date
MX2007003142A true MX2007003142A (es) 2007-07-04

Family

ID=36090646

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2007003142A MX2007003142A (es) 2004-09-21 2005-09-20 Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias.

Country Status (13)

Country Link
US (2) US7718410B2 (es)
EP (2) EP2770053B1 (es)
JP (2) JP2008513039A (es)
CN (1) CN101151366B (es)
AU (1) AU2005286722B2 (es)
BR (1) BRPI0515547A (es)
CA (1) CA2580840A1 (es)
ES (2) ES2666693T3 (es)
IL (3) IL182035A0 (es)
MX (1) MX2007003142A (es)
PL (1) PL1794323T3 (es)
PT (1) PT1794323E (es)
WO (1) WO2006034332A2 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
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MXPA03009566A (es) * 2001-04-19 2004-12-06 Scripps Research Inst Metodos y composicion para la produccion de pares trna-aminoaciltrna sintetasa ortogonales.
DE60329700D1 (en) * 2002-10-16 2009-11-26 Scripps Research Inst Glycoproteinsynthese
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
SI1666604T1 (sl) * 2003-07-07 2008-08-31 Scripps Research Inst Sestavki ortogonalnih parov lizil-tRNA in aminoacil-tRNA in aminoacil-tRNA-sintetaze in njihove uporabe
US20080171317A1 (en) * 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
EP2383340B1 (en) 2004-10-27 2014-07-16 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
KR20080069993A (ko) * 2005-10-12 2008-07-29 더 스크립스 리서치 인스티튜트 파지-디스플레이된 폴리펩티드의 선별적인 번역후 변형
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
EP1991680B1 (en) * 2006-03-09 2013-08-21 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
MX2008011669A (es) 2006-03-16 2008-09-22 Scripps Research Inst Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.
WO2007139870A1 (en) * 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
US8114652B2 (en) 2006-09-21 2012-02-14 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
RU2009112104A (ru) 2006-10-18 2010-11-27 Зе Скрипс Ресеч Инститьют (Us) Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих
CN102203257B (zh) * 2007-10-25 2014-01-08 斯克利普斯研究院 3-氨基酪氨酸遗传掺入还原酶
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
BRPI0819092A2 (pt) * 2007-11-02 2014-10-14 Scripps Research Inst Evolução direcionada usando proteínas que compreendem aminoácidos não naturais.
JP2011527886A (ja) 2007-12-11 2011-11-10 ザ スクリプス リサーチ インスティチュート メタノール資化性酵母ピキア・パストリスにおけるインビボ非天然アミノ酸発現
BRPI0906388A2 (pt) * 2008-02-08 2015-07-07 Scripps Research Inst Quebra de tolerância imunológica com um aminoácido não natural geneticamente codificado
CA2722858C (en) 2008-04-30 2017-08-29 Siemens Medical Solutions Usa, Inc. Substrate based pet imaging agents
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
CN102307905B (zh) * 2008-12-10 2015-11-25 斯克利普斯研究院 利用化学反应性非天然氨基酸产生载体-肽偶联物
GB2470770A (en) * 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
US8828395B2 (en) 2009-12-11 2014-09-09 Atyr Pharma, Inc. Antibodies that bind tyrosyl-tRNA synthetases
WO2012027611A2 (en) 2010-08-25 2012-03-01 Atyr Pharma, Inc. INNOVATIVE DISCOVERY OF THERAPEUTIC, DIAGNOSTIC, AND ANTIBODY COMPOSITIONS RELATED TO PROTEIN FRAGMENTS OF TYROSYL-tRNA SYNTHETASES
WO2013022982A2 (en) * 2011-08-09 2013-02-14 Atyr Pharma, Inc. Pegylated tyrosyl-trna synthetase polypeptides
CN108410827B (zh) 2012-05-18 2023-05-09 英国研究与创新署 整合包含bcn基团的氨基酸到多肽中的方法
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
WO2014116730A2 (en) 2013-01-23 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Stabilized hepatitis b core polypeptide
US9255255B2 (en) 2013-03-04 2016-02-09 The Board Of Trustees Of The Leland Stanford Junior University Synthesis of linear and branched polymers of polypeptides through direct conjugation
JO3564B1 (ar) 2013-03-08 2020-07-05 Novartis Ag ببتيدات وتركيبات لعلاج ضرر المفاصل
EP3021942A4 (en) 2013-07-19 2017-04-19 The Regents of The University of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
CN106084017B (zh) * 2016-06-13 2019-11-22 浙江大学 一种大肠杆菌水通道蛋白AQPZ中整合非天然氨基酸pPpa的方法
CN109963579A (zh) 2016-11-14 2019-07-02 诺华股份有限公司 用于治疗软骨损伤和关节炎的方法和组合物
WO2018087720A1 (en) 2016-11-14 2018-05-17 Novartis Ag Compositions, methods, and therapeutic uses related to fusogenic protein minion
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
JP7495420B2 (ja) 2019-02-20 2024-06-04 ノヴォ ノルディスク アー／エス アミノアシル－ｔＲＮＡ合成酵素およびこの使用
KR102152939B1 (ko) * 2019-04-30 2020-09-07 한국화학연구원 공유결합성 트리아졸로 연결된 가시광선 흡수 광촉매, 이의 제조방법 및 이를 이용한 아자이드-알카인 고리화 첨가반응으로 트리아졸 유도체를 제조하는 방법
JP2023537397A (ja) * 2020-08-12 2023-08-31 ケンブリッジ エンタープライズ リミテッド 標的化された核酸の切断方法
CN114107394B (zh) * 2021-11-05 2024-01-30 中国科学院精密测量科学与技术创新研究院 一种慢病毒转移载体、表达PylRS及tRNACUA的细胞系及制备方法与应用

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Publication number Priority date Publication date Assignee Title
MXPA03009566A (es) 2001-04-19 2004-12-06 Scripps Research Inst Metodos y composicion para la produccion de pares trna-aminoaciltrna sintetasa ortogonales.
DE60329700D1 (en) 2002-10-16 2009-11-26 Scripps Research Inst Glycoproteinsynthese
DE60327369D1 (de) 2002-10-16 2009-06-04 Scripps Research Inst Stellenspezifischer einbau von ketoaminosäuren in proteine
MXPA05006836A (es) 2002-12-22 2005-08-16 Scripps Research Inst Colecciones ordenadas de proteinas.
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
EP2392923B1 (en) 2003-06-18 2014-05-21 The Scripps Research Institute Method of producing proteins comprising unnatural amino acid
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
US7527943B2 (en) 2003-07-07 2009-05-05 The Scripps Research Institute Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
SI1666604T1 (sl) 2003-07-07 2008-08-31 Scripps Research Inst Sestavki ortogonalnih parov lizil-tRNA in aminoacil-tRNA in aminoacil-tRNA-sintetaze in njihove uporabe
EP2383340B1 (en) 2004-10-27 2014-07-16 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Also Published As

Publication number Publication date
ES2666693T3 (es) 2018-05-07
WO2006034332A2 (en) 2006-03-30
IL218089A (en) 2014-01-30
IL182035A0 (en) 2007-07-24
US20080220472A1 (en) 2008-09-11
EP1794323A2 (en) 2007-06-13
IL218090A0 (en) 2012-03-29
WO2006034332A3 (en) 2007-12-06
ES2519444T3 (es) 2014-11-07
PT1794323E (pt) 2014-10-30
JP5823941B2 (ja) 2015-11-25
EP2770053B1 (en) 2018-02-21
CA2580840A1 (en) 2006-03-30
CN101151366B (zh) 2012-12-19
EP1794323B1 (en) 2014-08-06
BRPI0515547A (pt) 2008-07-29
US7824893B2 (en) 2010-11-02
JP2013009682A (ja) 2013-01-17
IL218089A0 (en) 2012-03-29
PL1794323T3 (pl) 2015-02-27
JP2008513039A (ja) 2008-05-01
EP1794323A4 (en) 2008-08-27
IL218090A (en) 2014-02-27
EP2770053A1 (en) 2014-08-27
US7718410B2 (en) 2010-05-18
AU2005286722B2 (en) 2010-12-02
US20060073507A1 (en) 2006-04-06
CN101151366A (zh) 2008-03-26
AU2005286722A1 (en) 2006-03-30

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