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PT1991680E - Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas - Google Patents

Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas Download PDF

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Publication number
PT1991680E
PT1991680E PT77526010T PT07752601T PT1991680E PT 1991680 E PT1991680 E PT 1991680E PT 77526010 T PT77526010 T PT 77526010T PT 07752601 T PT07752601 T PT 07752601T PT 1991680 E PT1991680 E PT 1991680E
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PT
Portugal
Prior art keywords
expression
host cells
translation components
orthogonal translation
eubacterial host
Prior art date
Application number
PT77526010T
Other languages
English (en)
Inventor
Peter G Schultz
Youngha Ryu
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Publication of PT1991680E publication Critical patent/PT1991680E/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PT77526010T 2006-03-09 2007-03-07 Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas PT1991680E (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US78097306P 2006-03-09 2006-03-09
US78349706P 2006-03-17 2006-03-17
US85533606P 2006-10-29 2006-10-29

Publications (1)

Publication Number Publication Date
PT1991680E true PT1991680E (pt) 2013-10-30

Family

ID=38475556

Family Applications (1)

Application Number Title Priority Date Filing Date
PT77526010T PT1991680E (pt) 2006-03-09 2007-03-07 Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas

Country Status (14)

Country Link
US (3) US7960141B2 (pt)
EP (1) EP1991680B1 (pt)
JP (1) JP5589186B2 (pt)
KR (1) KR20080106430A (pt)
CN (1) CN101400796B (pt)
AU (1) AU2007223830A1 (pt)
BR (1) BRPI0709935A2 (pt)
CA (1) CA2638763A1 (pt)
ES (1) ES2432391T3 (pt)
IL (1) IL193394A0 (pt)
MX (1) MX2008011470A (pt)
PL (1) PL1991680T3 (pt)
PT (1) PT1991680E (pt)
WO (1) WO2007103490A2 (pt)

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DE60329700D1 (en) * 2002-10-16 2009-11-26 Scripps Research Inst Glycoproteinsynthese
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
SI1666604T1 (sl) * 2003-07-07 2008-08-31 Scripps Research Inst Sestavki ortogonalnih parov lizil-tRNA in aminoacil-tRNA in aminoacil-tRNA-sintetaze in njihove uporabe
US20080171317A1 (en) * 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
EP2383340B1 (en) 2004-10-27 2014-07-16 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
EP1991680B1 (en) * 2006-03-09 2013-08-21 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
MX2008011669A (es) 2006-03-16 2008-09-22 Scripps Research Inst Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.
WO2007139870A1 (en) 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
US8114652B2 (en) 2006-09-21 2012-02-14 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
RU2009112104A (ru) 2006-10-18 2010-11-27 Зе Скрипс Ресеч Инститьют (Us) Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
JP2011527886A (ja) * 2007-12-11 2011-11-10 ザ スクリプス リサーチ インスティチュート メタノール資化性酵母ピキア・パストリスにおけるインビボ非天然アミノ酸発現
BRPI0906388A2 (pt) * 2008-02-08 2015-07-07 Scripps Research Inst Quebra de tolerância imunológica com um aminoácido não natural geneticamente codificado
PT2337846T (pt) 2008-09-26 2018-04-05 Ambrx Inc Vacinas e microrganismos dependentes da replicação de aminoácidos não naturais
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
CN102307905B (zh) * 2008-12-10 2015-11-25 斯克利普斯研究院 利用化学反应性非天然氨基酸产生载体-肽偶联物
AU2011291943B2 (en) * 2010-08-17 2015-01-22 Ambrx, Inc. Modified relaxin polypeptides and their uses
KR20160027217A (ko) 2012-05-23 2016-03-09 에프. 호프만-라 로슈 아게 내배엽 및 간세포를 수득하고 사용하는 조성물 및 방법
JO3564B1 (ar) 2013-03-08 2020-07-05 Novartis Ag ببتيدات وتركيبات لعلاج ضرر المفاصل
EP3021942A4 (en) 2013-07-19 2017-04-19 The Regents of The University of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
WO2018087720A1 (en) 2016-11-14 2018-05-17 Novartis Ag Compositions, methods, and therapeutic uses related to fusogenic protein minion
CN109963579A (zh) 2016-11-14 2019-07-02 诺华股份有限公司 用于治疗软骨损伤和关节炎的方法和组合物
KR102670432B1 (ko) 2017-02-08 2024-05-28 브리스톨-마이어스 스큅 컴퍼니 약동학적 인핸서를 포함하는 변형된 렐락신 폴리펩티드 및 그의 용도
AU2020378327A1 (en) * 2019-11-05 2022-05-26 Nitro Biosciences, Inc. Biosynthesis of para-nitro-L-phenylalanine
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
CN113322267B (zh) * 2021-03-15 2024-01-05 天津大学 系统在提高非天然氨基酸的插入效率中的应用
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte

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DE60329700D1 (en) 2002-10-16 2009-11-26 Scripps Research Inst Glycoproteinsynthese
DE60327369D1 (de) * 2002-10-16 2009-06-04 Scripps Research Inst Stellenspezifischer einbau von ketoaminosäuren in proteine
MXPA05006836A (es) 2002-12-22 2005-08-16 Scripps Research Inst Colecciones ordenadas de proteinas.
CN103289960B (zh) * 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
EP2392923B1 (en) * 2003-06-18 2014-05-21 The Scripps Research Institute Method of producing proteins comprising unnatural amino acid
SI1666604T1 (sl) * 2003-07-07 2008-08-31 Scripps Research Inst Sestavki ortogonalnih parov lizil-tRNA in aminoacil-tRNA in aminoacil-tRNA-sintetaze in njihove uporabe
US7527943B2 (en) 2003-07-07 2009-05-05 The Scripps Research Institute Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
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JP2008513040A (ja) 2004-09-21 2008-05-01 ザ スクリップス リサーチ インスティテュート 光調節型アミノ酸の遺伝コード付加
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EP2383340B1 (en) * 2004-10-27 2014-07-16 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
KR20080069993A (ko) * 2005-10-12 2008-07-29 더 스크립스 리서치 인스티튜트 파지-디스플레이된 폴리펩티드의 선별적인 번역후 변형
EP1991680B1 (en) * 2006-03-09 2013-08-21 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
MX2008011669A (es) 2006-03-16 2008-09-22 Scripps Research Inst Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Also Published As

Publication number Publication date
US8357512B2 (en) 2013-01-22
CA2638763A1 (en) 2007-09-13
WO2007103490A3 (en) 2008-06-12
US20070281335A1 (en) 2007-12-06
EP1991680A2 (en) 2008-11-19
IL193394A0 (en) 2009-09-22
AU2007223830A1 (en) 2007-09-13
MX2008011470A (es) 2008-09-24
JP5589186B2 (ja) 2014-09-17
CN101400796B (zh) 2013-03-27
ES2432391T3 (es) 2013-12-03
US20090181429A1 (en) 2009-07-16
CN101400796A (zh) 2009-04-01
KR20080106430A (ko) 2008-12-05
US8198044B2 (en) 2012-06-12
BRPI0709935A2 (pt) 2011-08-02
US7960141B2 (en) 2011-06-14
US20120252067A1 (en) 2012-10-04
WO2007103490A2 (en) 2007-09-13
JP2009529328A (ja) 2009-08-20
PL1991680T3 (pl) 2014-01-31
EP1991680A4 (en) 2009-10-21
EP1991680B1 (en) 2013-08-21

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