CR9769A - Composiciones insecticidas y metodos para crear plantas transgenicas resistentes a insectos - Google Patents
Composiciones insecticidas y metodos para crear plantas transgenicas resistentes a insectosInfo
- Publication number
- CR9769A CR9769A CR9769A CR9769A CR9769A CR 9769 A CR9769 A CR 9769A CR 9769 A CR9769 A CR 9769A CR 9769 A CR9769 A CR 9769A CR 9769 A CR9769 A CR 9769A
- Authority
- CR
- Costa Rica
- Prior art keywords
- methods
- transgenic plants
- resistant transgenic
- insect resistant
- insecticide compositions
- Prior art date
Links
- 230000009261 transgenic effect Effects 0.000 title abstract 3
- 239000002917 insecticide Substances 0.000 title abstract 2
- 241000238631 Hexapoda Species 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 241000193388 Bacillus thuringiensis Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 229940097012 bacillus thuringiensis Drugs 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pest Control & Pesticides (AREA)
- Crystallography & Structural Chemistry (AREA)
- Insects & Arthropods (AREA)
- Pharmacology & Pharmacy (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
En la presente invencion se proveen secuencias de polinucleotidos aisladas que codifican proteinas ET37, TIC810 y TIC812 de Bacillus thuringiensis, y secuencias de nucleoticos para usar en la expresion de TIC809, ET37, TIC810 y TIC 812, y fusiones de diversas combinaciones de estas proteinas efectivas como insecticidas, tales como TIC127, en plantas. Se describen metodos para preparar y usar las secuencias de polinucleotidos y las porteinas en el desarrollo de celulas vegetales trangenicas y plantas transgenicas que presentan resistencia mejorada.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71311105P | 2005-08-31 | 2005-08-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CR9769A true CR9769A (es) | 2008-08-06 |
Family
ID=37698224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CR9769A CR9769A (es) | 2005-08-31 | 2008-02-27 | Composiciones insecticidas y metodos para crear plantas transgenicas resistentes a insectos |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9121035B2 (es) |
| EP (1) | EP1920060B1 (es) |
| CN (1) | CN101300353A (es) |
| AR (1) | AR057515A1 (es) |
| AT (1) | ATE505548T1 (es) |
| AU (1) | AU2006284856B2 (es) |
| BR (1) | BRPI0615649A2 (es) |
| CA (1) | CA2618430A1 (es) |
| CR (1) | CR9769A (es) |
| DE (1) | DE602006021318D1 (es) |
| EC (1) | ECSP088226A (es) |
| MX (1) | MX2008002801A (es) |
| RU (1) | RU2008112187A (es) |
| WO (1) | WO2007027776A2 (es) |
| ZA (1) | ZA200801477B (es) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060200878A1 (en) | 2004-12-21 | 2006-09-07 | Linda Lutfiyya | Recombinant DNA constructs and methods for controlling gene expression |
| US10501375B2 (en) | 2006-06-30 | 2019-12-10 | Corning Incorporated | Cordierite aluminum magnesium titanate compositions and ceramic articles comprising same |
| US8956436B2 (en) | 2006-06-30 | 2015-02-17 | Corning Incorporated | Cordierite aluminum magnesium titanate compositions and ceramic articles comprising same |
| EP2559767B1 (en) | 2006-10-12 | 2017-04-12 | Monsanto Technology LLC | Plant microRNAs and methods of use thereof |
| US10036036B1 (en) * | 2007-03-15 | 2018-07-31 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge as a seed blend |
| US8609936B2 (en) * | 2007-04-27 | 2013-12-17 | Monsanto Technology Llc | Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis |
| AU2009285624B2 (en) * | 2008-08-29 | 2013-12-05 | Monsanto Technology Llc | Novel Hemipteran and coleopteran active toxin proteins from Bacillus thuringiensis |
| MX383565B (es) * | 2008-10-15 | 2025-03-14 | Centro De Investigacion Y De Estudios Avanzados Del Instituto Politecnico Nac | Proteinas derivadas de genes cry de bacillus thuringiensis. |
| US8937214B2 (en) | 2009-10-23 | 2015-01-20 | Monsanto Technology Llc | Methods and compositions for expression of transgenes in plants |
| CN111269921B (zh) | 2011-04-07 | 2023-12-05 | 孟山都技术公司 | 具有对抗半翅目和/或鳞翅目昆虫的活性的昆虫抑制毒素家族 |
| WO2012174271A2 (en) | 2011-06-16 | 2012-12-20 | The Regents Of The University Of California | Synthetic gene clusters |
| BR112014003911A2 (pt) * | 2011-08-19 | 2017-03-14 | Synthetic Genomics Inc | método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso |
| DK3453711T3 (da) | 2011-09-02 | 2021-10-18 | Univ California | Llp2a-bisphosphonatkonjugater til behandling mod osteoporose |
| US9657308B2 (en) * | 2011-10-06 | 2017-05-23 | Dow Agrosciences Llc | Nucleic acid molecules that target PP1-87B and confer resistance to coleopteran pests |
| US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
| CA2866166C (en) | 2012-03-09 | 2023-10-17 | Vestaron Corporation | Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides |
| AR090622A1 (es) | 2012-04-06 | 2014-11-26 | Monsanto Technology Llc | Proteinas toxicas para especies de insectos hemipteros |
| US9688730B2 (en) | 2012-07-02 | 2017-06-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US10968446B2 (en) | 2012-11-01 | 2021-04-06 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
| CN102993282B (zh) * | 2012-11-19 | 2014-10-15 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
| WO2014182473A1 (en) * | 2013-05-08 | 2014-11-13 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge seed blend |
| CA2920339C (en) * | 2013-08-16 | 2023-10-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| BR112017010574B1 (pt) * | 2014-11-20 | 2024-01-30 | Monsanto Technology Llc | Cassete de expressão, célula hospedeira bacteriana, composição inibidora de inseto, método para controlar uma praga de espécie coleoptera, e vetor recombinante |
| TW201629221A (zh) * | 2014-12-16 | 2016-08-16 | 陶氏農業科學公司 | 以克魯拜爾基因(KRUPPEL GENE)之親代RNA干擾(RNAi)抑制作用來控制半翅目害蟲之技術 |
| BR102016005432A2 (pt) * | 2015-03-13 | 2016-09-13 | Dow Agrosciences Llc | moléculas de ácido nucléico de rna polimerase ii33 para controlar as pragas de inseto |
| BR102016005404A2 (pt) * | 2015-03-13 | 2016-09-20 | Dow Agrosciences Llc | moléculas de ácido nucléico de rna polimerase ii33 para controlar as pragas de inseto |
| BR112018000729B1 (pt) | 2015-07-13 | 2023-02-07 | Pivot Bio, Inc | Método de aumento de uma quantidade de nitrogênio atmosférico em uma planta de milho em um campo |
| HRP20211858T1 (hr) * | 2015-07-30 | 2022-03-04 | Monsanto Technology, Llc | Novi proteini za inhibiciju insekata |
| BR122023016256B8 (pt) | 2015-08-06 | 2024-04-30 | Du Pont | Polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, método de obtenção de uma planta transgênica ou célula de planta transgênica, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas e uso do polipeptídeo |
| BR112018003400A2 (pt) * | 2015-08-25 | 2018-09-25 | Dow Agrosciences Llc | toxinas cry inseticidas irdig17912 |
| CN108291219B (zh) | 2015-10-05 | 2023-02-17 | 麻省理工学院 | 使用重构nif簇的氮固定 |
| MX380615B (es) | 2015-11-02 | 2025-03-12 | Monsanto Technology Llc | Evento transgenico de algodon mon 88702 y metodos para su deteccion y usos. |
| US10612037B2 (en) | 2016-06-20 | 2020-04-07 | Monsanto Technology Llc | Insecticidal proteins toxic or inhibitory to hemipteran pests |
| EP3528617A4 (en) | 2016-10-21 | 2020-11-25 | Vestaron Corporation | CLIVABLE PEPTIDES AND PROTEINS, INSECTICIDES AND NEMATICIDES CONTAINING THEM |
| MX2019008285A (es) | 2017-01-12 | 2019-11-11 | Pivot Bio Inc | Métodos y composiciones para mejorar atributos de plantas. |
| MX2020004343A (es) | 2017-10-25 | 2021-01-08 | Pivot Bio Inc | Métodos y composiciones para mejorar microbios modificados que fijan nitrógeno. |
| MX2020004344A (es) | 2017-10-25 | 2020-10-14 | Pivot Bio Inc | Objetivos genéticos para el direccionamiento de fijación de nitrógeno para mejorar los rasgos de las plantas. |
| CN111526942B (zh) | 2017-11-27 | 2025-06-03 | 巴斯夫欧洲公司 | 设计成用于高通量筛选刺吸害虫如节肢动物的微量滴定板 |
| KR20200123144A (ko) | 2018-02-22 | 2020-10-28 | 지머젠 인코포레이티드 | 바실러스가 농축된 게놈 라이브러리를 생성하고 새로운 cry 독소를 동정하기 위한 방법 |
| BR112020017975A2 (pt) | 2018-03-02 | 2020-12-29 | Zymergen Inc. | Plataforma de descoberta de proteína inseticida e proteínas inseticidas descobertas a partir da mesma |
| US20190276840A1 (en) | 2018-03-09 | 2019-09-12 | James A. Baum | Methods and compositions for making and using compatible insecticidal proteins |
| WO2020006064A2 (en) | 2018-06-27 | 2020-01-02 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| MX2021000187A (es) | 2018-07-11 | 2021-06-15 | Pivot Bio Inc | Suministro de nitrógeno dinámico temporal y espacialmente dirigido mediante microbios remodelados. |
| WO2020190363A1 (en) | 2019-03-19 | 2020-09-24 | Massachusetts Institute Of Technology | Control of nitrogen fixation in rhizobia that associate with cereals |
| EP4143293A1 (en) | 2020-05-01 | 2023-03-08 | Pivot Bio, Inc. | Measurement of nitrogen fixation and incorporation |
| CN116676304B (zh) * | 2023-07-20 | 2023-09-26 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp016-1及其检测方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9007159A (pt) | 1989-02-24 | 1991-12-10 | Monsanto Co | Genes sinteticos de plantas e processo para a preparacao dos mesmos |
| US5849870A (en) * | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
| US5322687A (en) | 1993-07-29 | 1994-06-21 | Ecogen Inc. | Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects |
| US5723440A (en) * | 1995-06-07 | 1998-03-03 | Mycogen Corporation | Controlling hemipteran insect pests with Bacillus thuringiensis |
| US6093695A (en) * | 1996-09-26 | 2000-07-25 | Monsanto Company | Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP |
| US6063597A (en) | 1997-12-18 | 2000-05-16 | Monsanto Company | Polypeptide compositions toxic to coleopteran insects |
| CA2401958A1 (en) * | 2000-02-29 | 2001-09-07 | Auburn University | Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants |
-
2006
- 2006-08-30 US US12/064,840 patent/US9121035B2/en active Active
- 2006-08-30 CN CNA2006800404600A patent/CN101300353A/zh active Pending
- 2006-08-30 DE DE602006021318T patent/DE602006021318D1/de active Active
- 2006-08-30 MX MX2008002801A patent/MX2008002801A/es unknown
- 2006-08-30 CA CA002618430A patent/CA2618430A1/en not_active Abandoned
- 2006-08-30 RU RU2008112187/13A patent/RU2008112187A/ru not_active Application Discontinuation
- 2006-08-30 WO PCT/US2006/033867 patent/WO2007027776A2/en not_active Ceased
- 2006-08-30 EP EP06813950A patent/EP1920060B1/en active Active
- 2006-08-30 BR BRPI0615649-5A patent/BRPI0615649A2/pt not_active IP Right Cessation
- 2006-08-30 AT AT06813950T patent/ATE505548T1/de active
- 2006-08-30 AU AU2006284856A patent/AU2006284856B2/en not_active Ceased
- 2006-08-31 AR ARP060103814A patent/AR057515A1/es unknown
-
2008
- 2008-02-13 ZA ZA200801477A patent/ZA200801477B/xx unknown
- 2008-02-26 EC EC2008008226A patent/ECSP088226A/es unknown
- 2008-02-27 CR CR9769A patent/CR9769A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0615649A2 (pt) | 2011-05-24 |
| EP1920060A2 (en) | 2008-05-14 |
| CN101300353A (zh) | 2008-11-05 |
| AU2006284856B2 (en) | 2011-06-02 |
| ATE505548T1 (de) | 2011-04-15 |
| DE602006021318D1 (de) | 2011-05-26 |
| WO2007027776A2 (en) | 2007-03-08 |
| AU2006284856A1 (en) | 2007-03-08 |
| MX2008002801A (es) | 2008-04-07 |
| RU2008112187A (ru) | 2009-10-10 |
| WO2007027776A3 (en) | 2007-08-02 |
| CA2618430A1 (en) | 2007-03-08 |
| ECSP088226A (es) | 2008-03-26 |
| US20090068159A1 (en) | 2009-03-12 |
| AR057515A1 (es) | 2007-12-05 |
| ZA200801477B (en) | 2009-09-30 |
| US9121035B2 (en) | 2015-09-01 |
| EP1920060B1 (en) | 2011-04-13 |
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