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CL2012002007A1 - Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc. - Google Patents

Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc.

Info

Publication number
CL2012002007A1
CL2012002007A1 CL2012002007A CL2012002007A CL2012002007A1 CL 2012002007 A1 CL2012002007 A1 CL 2012002007A1 CL 2012002007 A CL2012002007 A CL 2012002007A CL 2012002007 A CL2012002007 A CL 2012002007A CL 2012002007 A1 CL2012002007 A1 CL 2012002007A1
Authority
CL
Chile
Prior art keywords
dna
plant
region
zinc finger
nuclease
Prior art date
Application number
CL2012002007A
Other languages
English (en)
Inventor
Sean Russel
Joseph F Petolino
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44307617&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2012002007(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of CL2012002007A1 publication Critical patent/CL2012002007A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265—Transgene containment, e.g. gene dispersal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para eliminar una región de ADN en una planta, que comprende proporcionar una planta que comprende dicha región de ADN en su genoma, la cual expresa una nucleasa de ""Dedo de Zinc"" diseñada para disociar el ADN genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc.
CL2012002007A 2010-01-22 2012-07-19 Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc. CL2012002007A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29762810P 2010-01-22 2010-01-22

Publications (1)

Publication Number Publication Date
CL2012002007A1 true CL2012002007A1 (es) 2012-10-19

Family

ID=44307617

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012002007A CL2012002007A1 (es) 2010-01-22 2012-07-19 Método para eliminar una región de adn en una planta, que comprende proporcionar una planta que comprende dicha región de adn en su genoma, la cual expresa una nucleasa de ‘dedo de zinc’ diseñada para disociar el adn genómico en una secuencia de reconocimiento; ácido nucleico aislado que comprende un gen de interés flanqueado por dos secuencias de reconocimiento para una nucleasa de dedo de zinc.

Country Status (20)

Country Link
US (3) US9695432B2 (es)
EP (1) EP2525649B1 (es)
JP (2) JP2013517774A (es)
KR (1) KR101951489B1 (es)
CN (1) CN102843904B (es)
AR (1) AR079968A1 (es)
AU (3) AU2011207387B2 (es)
BR (1) BR112012017896A2 (es)
CA (1) CA2787674C (es)
CL (1) CL2012002007A1 (es)
CO (1) CO6592070A2 (es)
EA (1) EA031356B1 (es)
GE (1) GEP201606544B (es)
IL (2) IL220870A (es)
MX (1) MX2012008485A (es)
NZ (1) NZ600546A (es)
PH (1) PH12012501467B1 (es)
UA (1) UA113493C2 (es)
WO (1) WO2011091311A2 (es)
ZA (1) ZA201204519B (es)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX336846B (es) * 2010-01-22 2016-02-03 Sangamo Biosciences Inc Alteracion genomica dirigida.
CA2831144A1 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
CN103890181A (zh) * 2011-08-22 2014-06-25 拜尔作物科学公司 修饰植物基因组的方法和手段
CN104427863B (zh) 2012-01-23 2019-06-28 陶氏益农公司 除草剂耐受性棉花事件pDAB4468.19.10.3
PT2847335T (pt) 2012-04-25 2018-09-28 Regeneron Pharma Alvejamento mediado por nuclease com vetores de alvejamento grandes
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
AR093979A1 (es) * 2012-12-13 2015-07-01 Dow Agrosciences Llc Metodos de deteccion de adn para actividad de nucleasa especifica de sitio
SMT201800671T1 (it) 2013-04-16 2019-01-11 Regeneron Pharma Modificazione mirata del genoma di ratto
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
CN105682452B (zh) 2013-09-04 2018-10-16 美国陶氏益农公司 用于在作物中确定供体插入的快速靶向分析
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
EP3066192B1 (en) 2013-11-04 2019-05-01 Dow AgroSciences LLC A universal donor system for gene targeting
CN105980568B (zh) 2013-12-11 2019-12-03 瑞泽恩制药公司 用于靶向修饰基因组的方法和组合物
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
EP3152312B1 (en) 2014-06-06 2020-02-12 Regeneron Pharmaceuticals, Inc. Methods and compositions for modifying a targeted locus
CA2956224A1 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
RU2734770C2 (ru) 2014-11-21 2020-10-23 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленной генетической модификации с использованием парных гидовых рнк
AU2016342380B2 (en) 2015-10-23 2022-04-07 President And Fellows Of Harvard College Nucleobase editors and uses thereof
ES2977547T3 (es) 2016-05-20 2024-08-26 Regeneron Pharma Métodos para romper la tolerancia inmunológica utilizando múltiples ARN guía
WO2017222779A1 (en) 2016-06-20 2017-12-28 Dow Agrosciences Llc Methodologies and compositions for creating targeted recombination and breaking linkage between traits
IL308426B1 (en) 2016-08-03 2025-11-01 Harvard College Adenosine nucleobase editors and uses thereof
JP7201153B2 (ja) 2016-08-09 2023-01-10 プレジデント アンド フェローズ オブ ハーバード カレッジ プログラム可能ｃａｓ９－リコンビナーゼ融合タンパク質およびその使用
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
CA3043019A1 (en) * 2016-11-21 2018-05-24 Dow Agrosciences Llc Site specific integration of a transgene using intra-genomic recombination via a non-homologous end joining repair pathway
EP3555285A4 (en) * 2016-12-14 2020-07-08 Dow AgroSciences LLC RECONSTRUCTION OF SITE SPECIFIC NUCLEASE BINDING SITES
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
KR20200121782A (ko) 2017-10-16 2020-10-26 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001342T5 (de) 2019-03-19 2022-01-13 President and Fellows of Harvard College Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
EP3986118A4 (en) * 2019-06-19 2023-10-04 Pioneer Hi-Bred International, Inc. COMPOSITIONS AND METHODS FOR IMPROVING CUSH BREAK TOLERANCE IN RAPS
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
EP4146804A1 (en) 2020-05-08 2023-03-15 The Broad Institute Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US20240011043A1 (en) * 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Generation of plants with improved transgenic loci by genome editing
US11242534B1 (en) 2020-07-31 2022-02-08 Inari Agriculture Technology, Inc. INHT31 transgenic soybean
US11214811B1 (en) 2020-07-31 2022-01-04 Inari Agriculture Technology, Inc. INIR6 transgenic maize
WO2022026554A1 (en) * 2020-07-31 2022-02-03 Inari Agriculture Technology, Inc. Inir12 transgenic maize
US12529062B2 (en) 2020-07-31 2026-01-20 Inari Agriculture Technology, Inc. INIR12 transgenic maize
WO2023086847A1 (en) 2021-11-10 2023-05-19 Encodia, Inc. Methods for barcoding macromolecules in individual cells

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5750871A (en) 1986-05-29 1998-05-12 Calgene, Inc. Transformation and foreign gene expression in Brassica species
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
ATE87032T1 (de) 1986-12-05 1993-04-15 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
US5359142A (en) 1987-01-13 1994-10-25 Monsanto Company Method for enhanced expression of a protein
US5322938A (en) 1987-01-13 1994-06-21 Monsanto Company DNA sequence for enhancing the efficiency of transcription
US5244802A (en) 1987-11-18 1993-09-14 Phytogen Regeneration of cotton
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6051753A (en) 1989-09-07 2000-04-18 Calgene, Inc. Figwort mosaic virus promoter and uses
ES2150900T3 (es) 1989-10-31 2000-12-16 Monsanto Co Promotor para plantas transgenicas.
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
JP3209744B2 (ja) 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
DK0604662T3 (da) 1992-07-07 2008-10-20 Japan Tobacco Inc Fremgangsmåde til transformering af monocotyledon
US7060876B2 (en) 1992-07-07 2006-06-13 Japan Tobacco Inc. Method for transforming monocotyledons
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
ATE267870T1 (de) 1993-09-03 2004-06-15 Japan Tobacco Inc Verfahren zur transformation von monocotyledonen mittels des schildes von unreifen embryonen
US5635055A (en) 1994-07-19 1997-06-03 Exxon Research & Engineering Company Membrane process for increasing conversion of catalytic cracking or thermal cracking units (law011)
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5723765A (en) 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5846797A (en) 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5850019A (en) 1996-08-06 1998-12-15 University Of Kentucky Research Foundation Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
AU4208797A (en) 1996-09-05 1998-03-26 Unilever Plc Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein
CA2275491A1 (en) 1997-01-20 1998-07-23 Plant Genetic Systems, N.V. Pathogen-induced plant promoters
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US5922564A (en) 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
IL137971A0 (en) 1998-02-20 2001-10-31 Zeneca Ltd Pollen specific promoter
AU762993C (en) 1998-02-26 2004-06-10 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
WO1999043819A1 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
JP2000083680A (ja) 1998-07-16 2000-03-28 Nippon Paper Industries Co Ltd 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ―
EP1141346A2 (en) 1999-01-14 2001-10-10 Monsanto Co. Soybean transformation method
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6677503B1 (en) 1999-06-23 2004-01-13 Pioneer Hi-Bred International, Inc. Sunflower anti-pathogene proteins and genes and their uses
WO2001064926A2 (en) * 2000-02-28 2001-09-07 Yale University Methods and compositions to reduce or eliminate transmission of a transgene
US6388170B1 (en) 2000-04-07 2002-05-14 University Of Kentucky Research Foundation Bidirectional promoters and methods related thereto
AU2002256240B2 (en) * 2001-04-17 2007-03-15 Icon Genetics, Inc. Ires enabled gene trapping in plants
BR0307383A (pt) * 2002-01-23 2005-04-26 Univ Utah Res Found Mutagênese cromossomica alvo utilizando nucleases de ramificações de zinco
US7525015B2 (en) 2002-02-07 2009-04-28 Hybrigene, Inc. Prevention of transgene escape in genetically modified perennials
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
SI2025756T1 (sl) 2003-11-18 2011-10-28 Bayer Bioscience Nv Izboljšana ciljana vstavitev DNA v rastlinah
WO2006105946A2 (en) 2005-04-04 2006-10-12 Bayer Bioscience N.V. Methods and means for removal of a selected dna sequence
WO2007014275A2 (en) * 2005-07-26 2007-02-01 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008145757A1 (en) 2007-05-31 2008-12-04 Vrije Universiteit Brussel Targeted genome modifications in plants
CA2691440A1 (en) * 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
CA2937438C (en) * 2007-09-27 2020-07-07 Dow Agrosciences Llc Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes
BRPI0908267B8 (pt) * 2008-02-29 2019-02-05 Monsanto Technology Llc método de produção de uma planta de milho tolerante a seca
CA2725674A1 (en) * 2008-05-28 2009-12-03 Kyoto University Selection marker gene and use thereof

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