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AR098300A1 - Locus óptimos de la soja - Google Patents

Locus óptimos de la soja

Info

Publication number
AR098300A1
AR098300A1 ARP140104139A ARP140104139A AR098300A1 AR 098300 A1 AR098300 A1 AR 098300A1 AR P140104139 A ARP140104139 A AR P140104139A AR P140104139 A ARP140104139 A AR P140104139A AR 098300 A1 AR098300 A1 AR 098300A1
Authority
AR
Argentina
Prior art keywords
ogl
seq
soy
locus
gene
Prior art date
Application number
ARP140104139A
Other languages
English (en)
Inventor
Sastry-Dent Lakshmi
Cao Zehui
Sriram Shreedharan
R Webb Steven
L Camper Debra
Michael Ainley W
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR098300A1 publication Critical patent/AR098300A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542Glycine max [soybean]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatuses For Bulk Treatment Of Fruits And Vegetables And Apparatuses For Preparing Feeds (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Soy Sauces And Products Related Thereto (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Reivindicación 1: Una secuencia recombinante que comprende: una secuencia de ácidos nucleicos de al menos 1 Kb y con al menos 90 % de identidad de secuencia con una secuencia no génica seleccionada del grupo que consiste en soy_OGL_1423 (SEQ ID Nº 639), soy_OGL_1434 (SEQ ID Nº 137), soy_OGL_4625 (SEQ ID Nº 76), soy_OGL_6362 (SEQ ID Nº 440), soy_OGL_308 (SEQ ID Nº 43), soy_OGL_307 (SEQ ID Nº 566), soy_OGL_310 (SEQ ID Nº 4236), soy_OGL_684 (SEQ ID Nº 47), soy_OGL_682 (SEQ ID Nº 2101), soy_OGL_685 (SEQ ID Nº 48) y un ADN de interés, donde el ADN de interés se inserta en dicha secuencia no génica para producir dicha secuencia recombinante. Reivindicación 11: Una planta de soja, parte de planta de soja o célula vegetal de soja que comprende una secuencia recombinante de cualquiera de las reivindicaciones 1 - 10. Reivindicación 12: Un método de producción de una célula vegetal transgénica que comprende un ADN de interés, el método comprende: a) seleccionar un locus genómico de soja no génica objetivo con al menos 90% identidad de secuencia del grupo que consiste en soy_OGL_1423 (SEQ ID Nº 639), soy_OGL_1434 (SEQ ID Nº 137), soy_OGL_4625 (SEQ ID Nº 76), soy_OGL_6362 (SEQ ID Nº 440), soy_OGL_308 (SEQ ID Nº 43), soy_OGL_307 (SEQ ID Nº 566), soy_OGL_310 (SEQ ID Nº 4236), soy_OGL_684 (SEQ ID Nº 47), soy_OGL_682 (SEQ ID Nº 2101) y soy_OGL_685 (SEQ ID Nº 48); b) seleccionar una nucleasa de sitio especifico que se une a dicho locus genémico de soja no génico objetivo y lo escinde, c) introducir dicha nucleasa de sitio específico en una célula vegetal de soja, d) introducir el ADN de interés en la célula vegetal, e) insertar el ADN de interés en dicho locus genómico de soja no génico objetivo y f) seleccionar las células vegetales transgénicas que comprenden el ADN de interés objetivo de dicho locus no génico. Reivindicación 24: Un locus genómico de soja no génico purificado de al menos 1 Kb y con al menos 90 % de identidad de secuencia con una secuencia no génica seleccionada del grupo que consiste en soy_OGL_1423 (SEQ ID Nº 639), soy_OGL_1434 (SEQ ID Nº 137), soy_OGL_4625 (SEQ ID Nº 76), soy_OGL_6362 (SEQ ID Nº 440), soy_OGL_308 (SEQ ID Nº 43), soy_OGL_307 (SEQ ID Nº 566), soy_OGL_310 (SEQ ID Nº 4236), soy_OGL_684 (SEQ ID Nº 47), soy_OGL_682 (SEQ ID Nº 2101) y soy_OGL_685 (SEQ ID Nº 48).
ARP140104139A 2013-11-04 2014-11-04 Locus óptimos de la soja AR098300A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361899602P 2013-11-04 2013-11-04

Publications (1)

Publication Number Publication Date
AR098300A1 true AR098300A1 (es) 2016-05-26

Family

ID=53005275

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140104139A AR098300A1 (es) 2013-11-04 2014-11-04 Locus óptimos de la soja

Country Status (19)

Country Link
US (5) US9909131B2 (es)
EP (2) EP3862434A1 (es)
JP (2) JP6560205B2 (es)
KR (1) KR102269374B1 (es)
CN (1) CN106232821A (es)
AR (1) AR098300A1 (es)
AU (3) AU2014341934B2 (es)
BR (1) BR102014027466B1 (es)
CA (2) CA3226233A1 (es)
CL (5) CL2016001063A1 (es)
IL (2) IL245304B (es)
MX (1) MX358066B (es)
NZ (3) NZ735257A (es)
RU (1) RU2016120636A (es)
TW (1) TWI672378B (es)
UA (1) UA121459C2 (es)
UY (1) UY35816A (es)
WO (1) WO2015066643A1 (es)
ZA (1) ZA201602546B (es)

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