MX2018000729A - Métodos para amplificar las secuencias de ácido nucleico. - Google Patents
Métodos para amplificar las secuencias de ácido nucleico.Info
- Publication number
- MX2018000729A MX2018000729A MX2018000729A MX2018000729A MX2018000729A MX 2018000729 A MX2018000729 A MX 2018000729A MX 2018000729 A MX2018000729 A MX 2018000729A MX 2018000729 A MX2018000729 A MX 2018000729A MX 2018000729 A MX2018000729 A MX 2018000729A
- Authority
- MX
- Mexico
- Prior art keywords
- double stranded
- nucleic acid
- dna
- transposon dna
- transposon
- Prior art date
Links
- 150000007523 nucleic acids Chemical group 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 7
- 239000012634 fragment Substances 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 102000008579 Transposases Human genes 0.000 abstract 3
- 108010020764 Transposases Proteins 0.000 abstract 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 abstract 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 abstract 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 1
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se proporcionan métodos para amplificación de ácido nucleico que incluyen poner en contacto un ácido nucleico de doble cadena con transposasas unidas a un transposón de ADN, en donde el transposón de ADN incluye un sitio de unión a transposasa y una secuencia promotora de polimerasa de ARN polimerasa, en donde el complejo de transposasas/transposón de ADN se une a las ubicaciones objetivo a lo largo del ácido nucleico de doble cadena y descompone el ácido nucleido de doble cadena en una pluralidad de fragmentos de doble cadena, cada fragmento de doble cadena tiene el transposón de ADN unido a cada extremo 5'del fragmento de doble cadena, extender los fragmentos de doble cadena a lo largo del transposón de ADN para hacer productos de extensión de doble cadena con secuencias promotoras de ARN polimerasa de doble cadena en cada extremo, poner en contacto los productos de extensión de doble cadena con una ARN polimerasa para hacer una pluralidad de transcripciones de ARN de cada producto de extensión de doble cadena, transcribir de forma inversa las transcripciones de ARN en un ADN de un copia de una sola cadena, formar cadenas complementarias en el ADN de copia de una sola cadena para formar una pluralidad de amplicones de ADN de doble cadena que corresponden a cada fragmento de doble cadena, en donde los amplicones de ADN de doble cadena se amplifican de manera lineal desde los fragmentos de doble cadena originales.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562193733P | 2015-07-17 | 2015-07-17 | |
| PCT/US2016/042394 WO2017015075A1 (en) | 2015-07-17 | 2016-07-15 | Methods of amplifying nucleic acid sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2018000729A true MX2018000729A (es) | 2018-09-06 |
Family
ID=57834769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018000729A MX2018000729A (es) | 2015-07-17 | 2016-07-15 | Métodos para amplificar las secuencias de ácido nucleico. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10894980B2 (es) |
| EP (1) | EP3325665B1 (es) |
| JP (1) | JP2018527947A (es) |
| CN (1) | CN108026575B (es) |
| AU (1) | AU2016297510B2 (es) |
| CA (1) | CA2992717A1 (es) |
| IL (1) | IL256905A (es) |
| MX (1) | MX2018000729A (es) |
| RU (1) | RU2018105835A (es) |
| WO (1) | WO2017015075A1 (es) |
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2016
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- 2016-07-15 JP JP2018521483A patent/JP2018527947A/ja active Pending
- 2016-07-15 US US15/745,251 patent/US10894980B2/en active Active
- 2016-07-15 CA CA2992717A patent/CA2992717A1/en not_active Abandoned
- 2016-07-15 RU RU2018105835A patent/RU2018105835A/ru not_active Application Discontinuation
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| IL256905A (en) | 2018-03-29 |
| CN108026575A (zh) | 2018-05-11 |
| US10894980B2 (en) | 2021-01-19 |
| WO2017015075A1 (en) | 2017-01-26 |
| JP2018527947A (ja) | 2018-09-27 |
| EP3325665A1 (en) | 2018-05-30 |
| CA2992717A1 (en) | 2017-01-26 |
| EP3325665A4 (en) | 2019-02-27 |
| AU2016297510A1 (en) | 2018-02-15 |
| RU2018105835A (ru) | 2019-08-19 |
| CN108026575B (zh) | 2022-08-19 |
| US20190002967A1 (en) | 2019-01-03 |
| EP3325665B1 (en) | 2020-09-02 |
| AU2016297510B2 (en) | 2021-09-09 |
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