[go: up one dir, main page]

AR126301A1 - Métodos para controlar el tamaño del meristema para el mejoramiento de cultivos - Google Patents

Métodos para controlar el tamaño del meristema para el mejoramiento de cultivos

Info

Publication number
AR126301A1
AR126301A1 ARP210100759A ARP210100759A AR126301A1 AR 126301 A1 AR126301 A1 AR 126301A1 AR P210100759 A ARP210100759 A AR P210100759A AR P210100759 A ARP210100759 A AR P210100759A AR 126301 A1 AR126301 A1 AR 126301A1
Authority
AR
Argentina
Prior art keywords
methods
fea2
nucleic acid
editing system
guide nucleic
Prior art date
Application number
ARP210100759A
Other languages
English (en)
Inventor
Anthony Nicholas
Tao Pei
Zhao Xu
Casi Schienebeck
Zhi-Ming Ding
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of AR126301A1 publication Critical patent/AR126301A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Paper (AREA)

Abstract

La presente invención se refiere a composiciones y métodos para modificar los genes FACIATED EAR2 (FEA2) en plantas, opcionalmente para modificar el tamaño del meristemo. La invención además se refiere a plantas que tienen un mayor número de hileras de granos producidas usando los métodos y composiciones de la invención. Reivindicación 12: Una célula de planta que comprende un sistema de edición, el sistema de edición comprende: (a) una proteína efectora CRISPR-Cas; y (b) un ácido nucleico guía que comprende una secuencia espaciadora con complementariedad con un gen diana endógeno que codifica una proteína FEA2. Reivindicación 58: Un sistema de edición de genes que comprende una proteína efectora CRISPR-Cas asociada con un ácido nucleico guía, en donde el ácido nucleico guía comprende una secuencia espaciadora que se une a un gen FEA2 endógeno.
ARP210100759A 2020-03-26 2021-03-26 Métodos para controlar el tamaño del meristema para el mejoramiento de cultivos AR126301A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202063000206P 2020-03-26 2020-03-26

Publications (1)

Publication Number Publication Date
AR126301A1 true AR126301A1 (es) 2023-10-04

Family

ID=75540048

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210100759A AR126301A1 (es) 2020-03-26 2021-03-26 Métodos para controlar el tamaño del meristema para el mejoramiento de cultivos

Country Status (9)

Country Link
US (3) US11999946B2 (es)
EP (1) EP4126915A1 (es)
CN (1) CN115605500A (es)
AR (1) AR126301A1 (es)
BR (1) BR112022019158A2 (es)
CA (1) CA3176888A1 (es)
MX (1) MX2022011761A (es)
UY (1) UY39145A (es)
WO (1) WO2021195458A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11999946B2 (en) 2020-03-26 2024-06-04 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
MX2022015107A (es) 2020-06-02 2023-03-01 Pairwise Plants Services Inc Metodos para controlar el tama?o del meristema para mejorar los cultivos.
UY39278A (es) 2020-06-17 2022-01-31 Pairwise Plants Services Inc Métodos para el control del tamaño del meristemo para la mejora de cultivos
CN119365481A (zh) * 2022-06-29 2025-01-24 成对植物服务股份有限公司 用于控制分生组织大小以进行作物改良的方法和组合物
US20240000031A1 (en) * 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
US20240060081A1 (en) * 2022-08-11 2024-02-22 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN120350062A (zh) * 2024-01-15 2025-07-22 佛山菽创科技有限公司 经遗传修饰的大豆植物

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US6040504A (en) 1987-11-18 2000-03-21 Novartis Finance Corporation Cotton promoter
CA1339684C (en) 1988-05-17 1998-02-24 Peter H. Quail Plant ubquitin promoter system
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
ATE186571T1 (de) 1991-08-27 1999-11-15 Novartis Ag Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
CA2319079A1 (en) 1998-02-20 1999-08-26 Zeneca Limited Pollen specific promoter
AU2001247554A1 (en) 2000-03-17 2001-10-03 Cold Spring Harbor Laboratory Novel inflorescence meristem development gene, promoter, and methods of use for same
AR044724A1 (es) 2000-03-27 2005-10-05 Syngenta Participations Ag Promotores del virus de la rizadura amarilla del cestrum
MXPA02009335A (es) 2000-03-27 2003-02-12 Syngenta Participations Ag Promotores del virus de la rizadura amarilla del cestrum.
EP1523491A4 (en) 2002-05-06 2006-02-22 Pioneer Hi Bred Int CLAVATA3-SIMILAR POLYNUCLEOTIDE SEQUENCES FROM MAIZE AND METHOD OF USE
DE602004031041D1 (de) 2003-10-06 2011-02-24 Syngenta Participations Ag In pflanzenplastiden funktionsfähiger promotor
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
WO2008062049A1 (en) 2006-11-24 2008-05-29 Cropdesign N.V. Transgenic plants comprising as transgene a class i tcp or clavata 1 (clv1) or cah3 polypeptide having increased seed yield and a method for making the same
RU2636037C2 (ru) 2012-02-01 2017-11-17 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Устойчивые к глифосату растения и способы, связанные с ними
WO2013138544A1 (en) 2012-03-14 2013-09-19 E. I. Du Pont De Nemours And Company Nucleotide sequences encoding fasciated ear4 (fea4) and methods of use thereof
CN103088054B (zh) 2012-12-29 2016-06-08 中国科学院植物研究所 一种干扰植物内源cle家族多肽激素的方法及小分子多肽激素的拮抗多肽
US9982053B2 (en) 2014-08-05 2018-05-29 MabQuest, SA Immunological reagents
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
JP7067793B2 (ja) 2015-10-23 2022-05-16 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基編集因子およびその使用
US10876129B2 (en) * 2016-02-12 2020-12-29 Ceres, Inc. Methods and materials for high throughput testing of mutagenized allele combinations
IL264565B2 (en) 2016-08-03 2024-07-01 Harvard College Adenosine nucleobase editors and uses thereof
CN108192912B (zh) 2017-01-13 2019-05-07 中国农业大学 一种诱导产生玉米母本单倍体的方法
US12171179B2 (en) 2018-07-23 2024-12-24 Pioneer Hi-Bred International, Inc. Methods and compositions to increase yield through modifications of FEA3 genomic locus and associated ligands
EP3976790A4 (en) * 2019-05-29 2023-06-28 Monsanto Technology LLC Methods and compositions for generating dominant alleles using genome editing
US11999946B2 (en) 2020-03-26 2024-06-04 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement

Also Published As

Publication number Publication date
CA3176888A1 (en) 2021-09-30
EP4126915A1 (en) 2023-02-08
UY39145A (es) 2021-10-29
BR112022019158A2 (pt) 2022-11-08
CN115605500A (zh) 2023-01-13
US20240279640A1 (en) 2024-08-22
US20240301400A1 (en) 2024-09-12
US11999946B2 (en) 2024-06-04
WO2021195458A1 (en) 2021-09-30
MX2022011761A (es) 2022-12-08
US20210301282A1 (en) 2021-09-30

Similar Documents

Publication Publication Date Title
AR126301A1 (es) Métodos para controlar el tamaño del meristema para el mejoramiento de cultivos
BR112017022852A2 (pt) polipeptídeo recombinante com atividade pesticida, composição, molécula de ácido nucleico recombinante, ácido nucleico recombinante, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo, planta, semente transgênica, método para proteger uma planta e método para aumentar o rendimento em uma planta
AR114014A1 (es) Sistema de edición de bases y método basado en proteína cpf1
PH12020551719A1 (en) Genes, constructs and maize event dp-202216-6
BR122019005253A8 (pt) Molécula de ácido nucleico recombinante, vetor, célula hospedeira, polipeptídeo recombinante com atividade pesticida, composição, bem como métodos para o controle de uma população de pragas para matar uma praga, para a produção de um polipeptídeo com atividade pesticida, para a proteção de uma planta de uma praga, e para aumentar o rendimento em uma planta
AR107854A1 (es) Proteínas defensina multiméricas y métodos relacionados
AR110075A1 (es) Un método para edición basal en plantas
BR112018002535A2 (pt) polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas ou uma praga e uso do polipeptídeo
AR021067A1 (es) Composiciones polipeptidicas toxicas para insectos del genero diabrotica y metodos de uso de las mismas
BR112012020921A2 (pt) genes de delta-endotoxina axmi218, axmi219, axmi220, axmi226, axmi227, axmi228, axmi229, axmi230 e axmi231 e métodos para sua utilização
MX2018004265A (es) Metodo para producir plantas de genoma modificado a partir de protoplastos de planta a alta eficiencia.
BR112018075865A2 (pt) aumento de crescimento e rendimento de planta usando uma sequência de fenilalanina amônia liase
UY38617A (es) Control epigenético programable de la expresión genética en plantas
AR091383A1 (es) Metodos para mejorar el rendimiento de plantas de cultivos resistentes a 2,4-d
BR112014015109A2 (pt) aumento da defesa de soja contra pragas
AR128957A1 (es) Plantas rosáceas de floración temprana con características mejoradas
BR112017022383A2 (pt) polipeptídeo recombinante, composição, molécula de ácido nucleico recombinante, ácido nucleico recombinante, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo, planta, semente transgênica da planta, método para proteger uma planta, método para aumentar o rendimento em uma planta
Hasanović et al. Seed Priming beyond stress adaptation: Broadening the agronomic horizon
AR112465A1 (es) Métodos y composiciones para la edición de genes de base viral en plantas
BR112017013633A2 (pt) polipeptídeo recombinante, molécula de ácido nucleico recombinante, composição, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo com atividade pesticida, planta, semente transgênica, método para proteger uma planta de uma praga de insetos, método para aumentar o rendimento em uma planta
AR127904A1 (es) Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
MX2024015770A (es) Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos
AR121670A1 (es) Métodos para mejorar la resistencia a la roya de la soja
AR108748A1 (es) Promotor vegetal y 3’ utr para la expresión de transgenes
AR128687A1 (es) Modificación de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento