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AR126901A1 - Células madre totipotentes inducidas y su método de preparación - Google Patents

Células madre totipotentes inducidas y su método de preparación

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Publication number
AR126901A1
AR126901A1 ARP220102319A ARP220102319A AR126901A1 AR 126901 A1 AR126901 A1 AR 126901A1 AR P220102319 A ARP220102319 A AR P220102319A AR P220102319 A ARP220102319 A AR P220102319A AR 126901 A1 AR126901 A1 AR 126901A1
Authority
AR
Argentina
Prior art keywords
totipotent stem
stem cells
cell
inhibitor
induced
Prior art date
Application number
ARP220102319A
Other languages
English (en)
Inventor
Sheng Ding
Kang Liu
Yanyan Hu
Yuanyuan Yang
Pengcheng Tan
Tianhua Ma
Original Assignee
Univ Tsinghua
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Filing date
Publication date
Application filed by Univ Tsinghua filed Critical Univ Tsinghua
Publication of AR126901A1 publication Critical patent/AR126901A1/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract

La invención se refiere a una combinación de agentes de reprogramación de moléculas pequeñas para inducir la generación de células madre totipotentes, un método para preparar las células madre totipotentes inducidas, y las células madre totipotentes inducidas generadas a partir de los mismos. Reivindicación 1: Una composición que comprende: (a) un activador de la vía de señalización de RA; y (b) uno o más de un inhibidor de GSK-3, un inhibidor de la vía de señalización de IKK, un inhibidor de HDAC, un inhibidor de histona metiltransferasa, un inhibidor de quinasa Src, un activador de AMPc, y un inhibidor del metabolismo celular. Reivindicación 2: Un equipo que comprende: (a) un activador de la vía de señalización de RA; y (b) uno o más de un inhibidor de GSK-3, un inhibidor de la vía de señalización de IKK, un inhibidor de HDAC, un inhibidor de histona metiltransferasa, un inhibidor de quinasa Src, un activador de AMPc, y un inhibidor del metabolismo celular. Reivindicación 12: Un medio de cultivo que comprende la composición de acuerdo con cualquiera de las reivindicaciones anteriores. Reivindicación 15: Un método para fabricar células madre totipotentes inducidas, que comprende cultivar células madre pluripotentes en el medio de cultivo de acuerdo con cualquiera de las reivindicaciones anteriores, para fabricar así las células madre totipotentes inducidas. Reivindicación 21: Un cultivo que comprende el medio de cultivo de acuerdo con cualquiera de las reivindicaciones anteriores y células madre pluripotentes. Reivindicación 23: Un cultivo que comprende el medio de cultivo de acuerdo con cualquiera de las reivindicaciones anteriores y células madre totipotentes. Reivindicación 25: Una célula madre totipotente inducida, caracterizada por uno o más de los siguientes: (a) aumento de la transcripción de uno o más marcadores de transcripción totipotentes seleccionados del grupo que consiste en MERVL, Zscan4c, Zscan4d, Zscan4f, Zfp352, Tcstv1, Tcstv3, Teme92, Gm6763; (b) transcripción reducida de uno o más marcadores de transcripción pluripotentes seleccionados del grupo que consiste en POU5f1, ZFP42, NANOG, KLF4, ESRRB; y (c) la capacidad de diferenciarse en tipos celulares extraembrionarios; preferentemente, las células madre totipotentes inducidas pueden fabricarse mediante el método de acuerdo con cualquiera de las reivindicaciones anteriores. Reivindicación 27: Un organismo derivado de la célula madre totipotente inducida de acuerdo con cualquiera de las reivindicaciones anteriores, preferentemente donde el organismo es un animal roedor o un animal mamífero. Reivindicación 28: Un organoide producido a partir de la célula madre totipotente inducida de acuerdo con cualquiera de las reivindicaciones anteriores. Reivindicación 29: Un tejido producido a partir de la célula madre totipotente inducida de acuerdo con cualquiera de las reivindicaciones anteriores, donde preferentemente el tejido es sangre. Reivindicación 30: Una célula diferenciada, diferenciada de la célula madre totipotente inducida de acuerdo con cualquiera de las reivindicaciones anteriores, preferentemente donde la célula diferenciada es una célula sanguínea o una célula inmunitaria, tal como una célula T o una célula NK.
ARP220102319A 2021-08-26 2022-08-26 Células madre totipotentes inducidas y su método de preparación AR126901A1 (es)

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CN202110989429 2021-08-26
CN202210654031 2022-06-09

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US (1) US20250263669A1 (es)
EP (1) EP4394030A4 (es)
JP (1) JP2024537607A (es)
CN (1) CN115725495A (es)
AR (1) AR126901A1 (es)
TW (1) TW202328432A (es)
WO (1) WO2023025302A1 (es)

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CN116676256A (zh) * 2023-06-07 2023-09-01 浙江大学 细胞快速化学重编程技术
WO2025170008A1 (ja) * 2024-02-07 2025-08-14 国立大学法人京都大学 成熟心筋細胞の製造方法
WO2025207779A1 (en) * 2024-03-28 2025-10-02 Pioneer Hi-Bred International, Inc. Kinase inhibitors for plant cell reprogramming

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JP5711149B2 (ja) * 2009-01-13 2015-04-30 ステムバイオス テクノロジーズ，インコーポレイテッド 非胚性幹細胞およびその使用
EP2986711A1 (en) * 2013-04-16 2016-02-24 Universiteit Maastricht Blastoid, cell line based artificial blastocyst
CN104278008B (zh) * 2013-07-12 2020-08-21 北京宏冠再生医学科技有限公司 一种通过小分子化合物处理来制备多潜能干细胞的方法、试剂盒和用途
US20170114323A1 (en) * 2014-06-19 2017-04-27 Whitehead Institute For Biomedical Research Uses of kinase inhibitors for inducing and maintaining pluripotency
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US20190282624A1 (en) * 2015-11-30 2019-09-19 Beihao Stem Cell And Regenerative Medicine Research Institute Co., Ltd. Improved methods for reprograming non-pluripotent cells into pluripotent stem cells
CN110832069B (zh) * 2017-05-31 2023-06-20 北昊干细胞与再生医学研究院有限公司 用于化学诱导的谱系重编程的方法
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CN115725495A (zh) 2023-03-03
TW202328432A (zh) 2023-07-16
WO2023025302A1 (zh) 2023-03-02
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