AR125393A2 - Métodos y composiciones para la expresión génica en plantas - Google Patents
Métodos y composiciones para la expresión génica en plantasInfo
- Publication number
- AR125393A2 AR125393A2 ARP220101021A ARP220101021A AR125393A2 AR 125393 A2 AR125393 A2 AR 125393A2 AR P220101021 A ARP220101021 A AR P220101021A AR P220101021 A ARP220101021 A AR P220101021A AR 125393 A2 AR125393 A2 AR 125393A2
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- herbicide
- tolerant
- dna molecule
- recombinant dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 230000014509 gene expression Effects 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 20
- 239000004009 herbicide Substances 0.000 abstract 10
- 102000053602 DNA Human genes 0.000 abstract 9
- 230000002363 herbicidal effect Effects 0.000 abstract 9
- 108020004511 Recombinant DNA Proteins 0.000 abstract 8
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 108020004414 DNA Proteins 0.000 abstract 5
- 230000009261 transgenic effect Effects 0.000 abstract 5
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 4
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8221—Transit peptides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
- C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/07—Fusion polypeptide containing a localisation/targetting motif containing a mitochondrial localisation signal
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La invención proporciona moléculas de ADN recombinante útiles para proporcionar la expresión eficaz de proteínas en plantas transgénicas, así como composiciones y métodos para utilizar las moléculas de ADN recombinante. En modalidades particulares, la invención proporciona moléculas de ADN recombinante y construcciones que comprenden secuencias que codifican péptidos de tránsito y secuencias unidas operativamente que confieren tolerancia a herbicidas. Reivindicación 1: Una molécula de ADN recombinante que comprende una secuencia de ADN que codifica un péptido de tránsito unida operativamente a una secuencia de ADN que codifica una proteína con tolerancia a herbicidas heteróloga, caracterizada porque el péptido de tránsito comprende una secuencia de aminoácidos que comprende al menos 97 por ciento de identidad con la secuencia de SEQ ID Nº 265. Reivindicación 7: Una construcción de ADN, caracterizada porque comprende la molécula de ADN de la reivindicación 1 unida operativamente a un promotor heterólogo. Reivindicación 11: Una proteína recombinante, caracterizada porque comprende lo siguiente en unión operativa: a) un péptido de tránsito que comprende una secuencia de aminoácidos que comprende al menos 95 por ciento de identidad con la secuencia de SEQ ID Nº 265; y b) una proteína con tolerancia a herbicidas heteróloga. Reivindicación 13: Un método para producir una planta tolerante a herbicidas, caracterizado porque comprende las siguientes etapas: a) transformar una célula de planta con la molécula de ADN recombinante de la reivindicación 1; y b) regenerar a partir de esta una planta tolerante a herbicidas que comprende la molécula de ADN. Reivindicación 18: Un método para producir una planta o una semilla transgénica tolerante a herbicidas, caracterizado porque comprende cruzar una planta que comprende la molécula de ADN recombinante de la reivindicación 1 consigo misma o una segunda planta para producir una planta o una semilla transgénica tolerante a herbicidas. Reivindicación 19: Un método para expresar una proteína con tolerancia a herbicidas heteróloga en una planta o una célula, caracterizado porque el método comprende el cultivo de una planta o una célula que comprende la molécula de ADN recombinante de la reivindicación 1, en donde dicho cultivo da lugar a la expresión de la proteína con tolerancia a herbicidas heteróloga. Reivindicación 21: Un método para controlar o impedir el crecimiento de malezas en un área de crecimiento de una planta, caracterizado porque comprende aplicar una cantidad eficaz de al menos un herbicida PPO a un área de crecimiento de una planta que comprende una planta o una semilla transgénica que comprende la molécula de ADN de la reivindicación 1, en donde la planta o la semilla transgénica es tolerante al herbicida PPO. Reivindicación 23: Un método para controlar el crecimiento de malezas tolerantes a herbicidas, caracterizado porque comprende: a) cultivar en un área de crecimiento de una planta una planta o una semilla que comprende la molécula de ADN de la reivindicación 1; y b) aplicar un herbicida PPO y al menos otro herbicida al área de crecimiento de la planta, en donde la planta o la semilla es tolerante al herbicida PPO y el al menos un herbicida adicional. Reivindicación 27: Una molécula de ADN recombinante que comprende una secuencia de ADN que codifica un péptido de tránsito unida operativamente a una secuencia de ADN que codifica una proteína con tolerancia a herbicidas heteróloga, caracterizada porque el péptido de tránsito comprende una secuencia de aminoácidos que comprende al menos 95 por ciento de identidad con la secuencia de SEQ ID Nº 265.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662368840P | 2016-07-29 | 2016-07-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR125393A2 true AR125393A2 (es) | 2023-07-12 |
Family
ID=61016595
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP170102148A AR109196A1 (es) | 2016-07-29 | 2017-07-28 | Métodos y composiciones para la expresión génica en plantas |
| ARP220101020A AR125392A2 (es) | 2016-07-29 | 2022-04-20 | Métodos y composiciones para la expresión génica en plantas |
| ARP220101021A AR125393A2 (es) | 2016-07-29 | 2022-04-20 | Métodos y composiciones para la expresión génica en plantas |
| ARP220101022A AR125394A2 (es) | 2016-07-29 | 2022-04-20 | Métodos y composiciones para la expresión génica en plantas |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP170102148A AR109196A1 (es) | 2016-07-29 | 2017-07-28 | Métodos y composiciones para la expresión génica en plantas |
| ARP220101020A AR125392A2 (es) | 2016-07-29 | 2022-04-20 | Métodos y composiciones para la expresión génica en plantas |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220101022A AR125394A2 (es) | 2016-07-29 | 2022-04-20 | Métodos y composiciones para la expresión génica en plantas |
Country Status (19)
| Country | Link |
|---|---|
| US (3) | US10745712B2 (es) |
| EP (2) | EP3797583A3 (es) |
| JP (1) | JP7118047B2 (es) |
| KR (1) | KR102587506B1 (es) |
| CN (1) | CN109475099A (es) |
| AR (4) | AR109196A1 (es) |
| AU (2) | AU2017301813B2 (es) |
| CA (1) | CA2974824A1 (es) |
| CL (2) | CL2019000220A1 (es) |
| CO (1) | CO2019001678A2 (es) |
| EC (1) | ECSP19013893A (es) |
| MX (3) | MX386599B (es) |
| PE (2) | PE20240136A1 (es) |
| PH (1) | PH12019500209A1 (es) |
| RU (1) | RU2763534C2 (es) |
| UA (1) | UA127343C2 (es) |
| UY (2) | UY37347A (es) |
| WO (1) | WO2018022777A1 (es) |
| ZA (1) | ZA201900225B (es) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10370677B2 (en) | 2015-08-03 | 2019-08-06 | Monsanto Technology Llc | Methods and compositions for herbicide tolerance in plants |
| US10378023B2 (en) | 2015-09-01 | 2019-08-13 | Monsanto Technology Llc | Methods and compositions for herbicide tolerance in plants |
| US10563220B2 (en) | 2015-12-21 | 2020-02-18 | Monsanto Technology Llc | Compositions and methods for efficient targeting of transgenes |
| JP7118047B2 (ja) | 2016-07-29 | 2022-08-15 | モンサント テクノロジー エルエルシー | 植物における遺伝子発現のための方法及び組成物 |
| CA3026528A1 (en) | 2017-12-15 | 2019-06-15 | Monsanto Technology Llc | Methods and compositions for ppo herbicide tolerance |
| US11895994B2 (en) | 2019-10-27 | 2024-02-13 | Shanghai Raas Blood Products Co., Ltd. | Humanized knock-in mouse expressing human Protein C |
| CN111296364B (zh) * | 2019-10-27 | 2022-06-24 | 上海莱士血液制品股份有限公司 | 一种基因改造的小鼠动物模型基因改造方法及其应用 |
| CN121109454A (zh) * | 2019-11-06 | 2025-12-12 | 青岛清原化合物有限公司 | 在生物体内创制新基因的方法及应用 |
| CN111218469B (zh) * | 2020-02-05 | 2022-03-22 | 中国农业科学院油料作物研究所 | 一种双T-DNA载体pBID-RT Enhanced及其构建方法和应用 |
| CN115336534A (zh) * | 2021-05-12 | 2022-11-15 | 北京大北农生物技术有限公司 | 原卟啉原氧化酶的用途 |
| WO2023044364A1 (en) | 2021-09-15 | 2023-03-23 | Enko Chem, Inc. | Protoporphyrinogen oxidase inhibitors |
| PE20241633A1 (es) | 2022-01-14 | 2024-08-09 | Enko Chem Inc | Inhibidores de protoporfirinogeno oxidasa |
| KR20250034947A (ko) | 2022-07-13 | 2025-03-11 | 바스프 에스이 | 아진 화합물을 포함하는 제초제 조성물 |
| WO2024015949A2 (en) * | 2022-07-14 | 2024-01-18 | Monsanto Technology Llc | Methods and compositions for ppo herbicide tolerance |
| WO2024218220A1 (en) | 2023-04-19 | 2024-10-24 | Syngenta Crop Protection Ag | Herbicide resistant plants |
| GB202314578D0 (en) | 2023-09-22 | 2023-11-08 | Univ Manchester | Methods of producing homoplasmic modified plants or parts thereof |
| WO2025114052A1 (en) | 2023-11-29 | 2025-06-05 | Basf Se | Ternary herbicidal composition comprising azine compounds |
| WO2025118900A1 (zh) * | 2023-12-07 | 2025-06-12 | 青岛清原种子科学有限公司 | HemG PPO酶在产生或提高对PPO抑制性除草剂耐受性上的应用 |
| WO2025153595A1 (en) | 2024-01-17 | 2025-07-24 | Basf Se | Plants having increased tolerance to herbicides |
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| US5939602A (en) | 1995-06-06 | 1999-08-17 | Novartis Finance Corporation | DNA molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof |
| US6023012A (en) * | 1996-02-28 | 2000-02-08 | Novartis Finance Corporation | DNA molecules encoding plant protoporphyrinogen oxidase |
| US6084155A (en) | 1995-06-06 | 2000-07-04 | Novartis Ag | Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes |
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| ZA98371B (en) | 1997-01-31 | 1999-07-16 | Du Pont | Genetically transformed plants demonstrating resistance to porphyrinogen biosynthesis-inhibiting herbicides. |
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| US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
| WO2001026458A2 (en) | 1999-10-11 | 2001-04-19 | Back Kyoung Whan | Process for increasing crop yield or biomass using protoporphyrinogen oxidase gene |
| AU2001260114A1 (en) * | 2000-03-14 | 2001-09-24 | Syngenta Participations Ag | Protoporphyrinogen oxidase ("protox") genes |
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| US10308953B2 (en) | 2013-12-18 | 2019-06-04 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
| CN103725653B (zh) * | 2013-12-26 | 2015-12-30 | 北京大北农科技集团股份有限公司 | 除草剂抗性蛋白质、其编码基因及用途 |
| CN107466321B (zh) | 2014-12-16 | 2021-07-20 | 福阿母韩农株式会社 | 通过原卟啉原氧化酶变体赋予或增强植物和/或藻类抵抗除草剂的方法 |
| US20190062777A1 (en) | 2015-06-17 | 2019-02-28 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
| US10370677B2 (en) | 2015-08-03 | 2019-08-06 | Monsanto Technology Llc | Methods and compositions for herbicide tolerance in plants |
| US10378023B2 (en) | 2015-09-01 | 2019-08-13 | Monsanto Technology Llc | Methods and compositions for herbicide tolerance in plants |
| US10563220B2 (en) | 2015-12-21 | 2020-02-18 | Monsanto Technology Llc | Compositions and methods for efficient targeting of transgenes |
| BR112018072473A2 (pt) | 2016-05-20 | 2019-02-19 | Basf Agro Bv | molécula de ácido nucleico, cassete de expressão, célula vegetal, planta, semente, métodos para expressar um ácido nucleico, produzir uma planta, controlar vegetação e cultivar a planta, combinação útil para o controle, processo para preparar uma combinação e uso de uma combinação |
| JP7118047B2 (ja) | 2016-07-29 | 2022-08-15 | モンサント テクノロジー エルエルシー | 植物における遺伝子発現のための方法及び組成物 |
| CA3026528A1 (en) | 2017-12-15 | 2019-06-15 | Monsanto Technology Llc | Methods and compositions for ppo herbicide tolerance |
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