AR102300A1 - Proteínas tóxicas o inhibidoras de insectos lepidópteros - Google Patents
Proteínas tóxicas o inhibidoras de insectos lepidópterosInfo
- Publication number
- AR102300A1 AR102300A1 ARP150103360A ARP150103360A AR102300A1 AR 102300 A1 AR102300 A1 AR 102300A1 AR P150103360 A ARP150103360 A AR P150103360A AR P150103360 A ARP150103360 A AR P150103360A AR 102300 A1 AR102300 A1 AR 102300A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- amino acid
- acid sequence
- proteins
- pest
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 9
- 102000004169 proteins and genes Human genes 0.000 title abstract 9
- 241000238631 Hexapoda Species 0.000 title 1
- 239000003112 inhibitor Substances 0.000 title 1
- 231100000331 toxic Toxicity 0.000 title 1
- 230000002588 toxic effect Effects 0.000 title 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 5
- 230000000361 pesticidal effect Effects 0.000 abstract 4
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 241000607479 Yersinia pestis Species 0.000 abstract 3
- 241000255777 Lepidoptera Species 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- 239000002917 insecticide Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108700012359 toxins Proteins 0.000 abstract 2
- 206010061217 Infestation Diseases 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000000749 insecticidal effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000000575 pesticide Substances 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 231100000654 protein toxin Toxicity 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Insects & Arthropods (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Agronomy & Crop Science (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Se divulgan secuencias de nucleótidos que codifican proteínas insecticidas TIC2160 y TIC3244 y variantes de estas, así como proteínas relacionadas que exhiben actividad inhibidora contra lepidópteros y fragmentos de estas. Las modalidades particulares proporcionan composiciones y plantas, partes de plantas y semillas transformadas que contienen un constructo polinucleótido que codifica una o más de las proteínas toxinas dentro de la clase de toxinas proteicas relacionadas con TIC2160. Reivindicación 1: Una molécula de ácido nucleico recombinante que comprende un promotor heterólogo unido que forma operativa a un segmento de polinucleótido que codifica una proteína pesticida o fragmento de esta, donde: a) dicha proteína pesticida comprende la secuencia de aminoácidos de la SEQ ID Nº 2, SEQ ID Nº 35, SEQ ID Nº 37, SEQ ID Nº 39, SEQ ID Nº 10, SEQ ID Nº 41, SEQ ID Nº 23, SEQ ID Nº 25, SEQ ID Nº 27, SEQ ID Nº 29, SEQ ID Nº 31, SEQ ID Nº 33 o SEQ ID No: 4; o b) dicha proteína pesticida comprende una secuencia de aminoácidos que tiene al menos desde aproximadamente 81% hasta aproximadamente 100% de identidad de secuencia de aminoácidos, o cualquier fracción de porcentaje entre 81% y 100%, con las proteínas seleccionadas del grupo que consiste en la SEQ ID Nº 2, SEQ ID Nº 35, SEQ ID Nº 37, SEQ ID Nº 39, SEQ ID Nº 10, SEQ ID Nº 41, SEQ ID Nº 23, SEQ ID Nº 25, SEQ ID Nº 27, SEQ ID Nº 29, SEQ ID Nº 31, SEQ ID Nº 33 y SEQ ID Nº 4; o c) dicho segmento de polinucleótido se híbrida en condiciones de hibridización rigurosas a un polinucleótido que tiene una secuencia de nucleótidos de la SEQ ID Nº 1, SEQ ID Nº 6, SEQ ID Nº 34, SEQ, ID Nº 36, SEQ ID Nº 38, SEQ ID Nº 11, SEQ ID Nº 40, SEQ ID Nº 22, SEQ ID Nº 24, SEQ ID Nº 26, SEQ ID Nº 28, SEQ ID Nº 30, SEQ ID Nº 32, SEQ ID Nº 3 o SEQ ID Nº 5 en condiciones de hibridización de 68ºC, seguida por un lavado a 68ºC, en 2xSSC que contiene SDS al 0,1%; o d) dicha molécula de ácido nucleico recombinante esta unida de forma operativa a un vector, y dicho vector se selecciona del grupo que consiste en un plásmido, fagémido, bácmido, cósmido y un cromosoma artificial bacteriano o de levadura. Reivindicación 22: Un método para controlar una plaga de especies de lepidópteros y controlar una infestación de especies de lepidópteros en una planta, donde dicho método comprende: a) poner en contacto la plaga con una cantidad eficaz como insecticida. de una proteína, pesticida como se establece en la SEQ ID Nº 2, SEQ ID Nº 35, SEQ ID Nº 37, SEQ ID Nº 39, SEQ ID Nº 10, SEQ ID Nº 41, SEQ ID Nº 23, SEQ ID Nº 25, SEQ ID Nº 27, SEQ ID Nº 29, SEQ ID Nº 31, SEQ ID Nº 33 o SEQ ID Nº 4; o b) poner en contacto la plaga con una cantidad eficaz como insecticida de una o más proteínas pesticidas que comprende una secuencia de aminoácidos que tiene al menos 81%, 85%, 90%, 95% o aproximadamente 100% de identidad de secuencia de aminoácidos con respecto a la SEQ ID Nº 2, SEQ ID Nº 35, SEQ ID Nº 37, SEQ ID Nº 39, SEQ ID Nº 10, SEQ ID Nº 41, SEQ ID Nº 23, SEQ ID Nº 25, SEQ ID Nº 27, SEQ ID Nº; 29, SEQ ID Nº 31, SEQ ID Nº 33 o SEQ ID Nº 4.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462064998P | 2014-10-16 | 2014-10-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR102300A1 true AR102300A1 (es) | 2017-02-15 |
Family
ID=54601994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150103360A AR102300A1 (es) | 2014-10-16 | 2015-10-16 | Proteínas tóxicas o inhibidoras de insectos lepidópteros |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US10316329B2 (es) |
| AR (1) | AR102300A1 (es) |
| UY (1) | UY36361A (es) |
| WO (1) | WO2016061392A1 (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017030808A1 (en) | 2015-08-18 | 2017-02-23 | Monsanto Technology Llc | Novel insect inhibitory proteins |
| CU24542B1 (es) | 2015-08-27 | 2021-08-06 | Monsanto Technology Llc | Proteínas inhibidoras de insectos provenientes de paenibacillus popilliae |
| WO2017079266A1 (en) * | 2015-11-02 | 2017-05-11 | Monsanto Technology Llc | Cotton transgenic event mon 88702 and methods for detection and uses thereof |
| CN106497966B (zh) * | 2016-10-12 | 2020-01-10 | 北京大北农科技集团股份有限公司 | 杀虫蛋白的用途 |
| CR20240356A (es) * | 2016-12-20 | 2024-10-04 | Monsanto Technology Llc | Proteínas inhibitorias de insectos noveles |
| CR20190367A (es) * | 2017-01-12 | 2019-09-25 | Monsanto Technology Llc | Proteínas toxinas pesticidas activas contra insectos lepidópteros |
| US10743535B2 (en) | 2017-08-18 | 2020-08-18 | H&K Solutions Llc | Insecticide for flight-capable pests |
| WO2022125639A1 (en) | 2020-12-08 | 2022-06-16 | Monsanto Technology Llc | Modified plant-associated bacteria and methods of their use |
| EP4262400A4 (en) | 2020-12-21 | 2025-01-08 | Monsanto Technology LLC | NEW INSECT INHIBITORY PROTEINS |
| UY39585A (es) | 2020-12-23 | 2022-07-29 | Monsanto Technology Llc | Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas |
| WO2022146874A1 (en) | 2020-12-31 | 2022-07-07 | Monsanto Technology Llc | Novel insect inhibitory proteins |
| CA3226147A1 (en) | 2021-07-08 | 2023-01-12 | Monsanto Technology Llc | Novel insect inhibitory proteins |
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| FR2673643B1 (fr) | 1991-03-05 | 1993-05-21 | Rhone Poulenc Agrochimie | Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide. |
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-
2015
- 2015-10-15 WO PCT/US2015/055802 patent/WO2016061392A1/en not_active Ceased
- 2015-10-15 US US14/884,456 patent/US10316329B2/en active Active
- 2015-10-16 AR ARP150103360A patent/AR102300A1/es not_active Application Discontinuation
- 2015-10-16 UY UY0001036361A patent/UY36361A/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| UY36361A (es) | 2016-06-01 |
| WO2016061392A1 (en) | 2016-04-21 |
| US10316329B2 (en) | 2019-06-11 |
| US20160108427A1 (en) | 2016-04-21 |
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