AR116615A2 - Operaciones de cosecha mejoradas para proteínas recombinadas - Google Patents
Operaciones de cosecha mejoradas para proteínas recombinadasInfo
- Publication number
- AR116615A2 AR116615A2 ARP190102873A ARP190102873A AR116615A2 AR 116615 A2 AR116615 A2 AR 116615A2 AR P190102873 A ARP190102873 A AR P190102873A AR P190102873 A ARP190102873 A AR P190102873A AR 116615 A2 AR116615 A2 AR 116615A2
- Authority
- AR
- Argentina
- Prior art keywords
- recombinant protein
- host cell
- prokaryotic host
- filtered volume
- dhna
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 238000013340 harvest operation Methods 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 9
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 9
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 238000003306 harvesting Methods 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 101150087021 menE gene Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente contempla métodos para la producción de una proteína recombinante que comprende (a) la fermentación de una célula huésped procariota, donde dicha célula huésped procariota ha sido transformada con un ácido nucleico que codifica dicha proteína recombinante, la cosecha de dicha proteína recombinante en condiciones donde los niveles de dO₂ son superiores a 0%, la purificación de dicha proteína recombinante hasta un volumen filtrado, donde dicho volumen filtrado no contiene una cantidad detectable de aducto do DHNA-proteína recombinante, medida por un ensayo de IEC a 310 nm. La presente además contempla un método para la producción de una proteína recombinante que comprende la fermentación de una célula huésped procariota con deleción del gen menE, donde dicha célula huésped procariota ha sido transformada con un ácido nucleico que codifica dicha proteína recombinante, la cosecha de dicha proteína recombinante; y la purificación de dicha proteína recombinante hasta un volumen filtrado, donde dicho volumen filtrado no contiene una cantidad detectable de aducto de DHNA-proteína recombinante, medida por un ensayo de IEC a 310 nm, y donde el rendimiento de proteína recombinante se incrementa alrededor de 20% o más.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261616297P | 2012-03-27 | 2012-03-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR116615A2 true AR116615A2 (es) | 2021-05-26 |
Family
ID=48040433
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130100828A AR090340A1 (es) | 2012-03-27 | 2013-03-14 | Operaciones de cosecha mejoradas para proteinas recombinadas |
| ARP190102873A AR116615A2 (es) | 2012-03-27 | 2019-10-09 | Operaciones de cosecha mejoradas para proteínas recombinadas |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130100828A AR090340A1 (es) | 2012-03-27 | 2013-03-14 | Operaciones de cosecha mejoradas para proteinas recombinadas |
Country Status (20)
| Country | Link |
|---|---|
| US (9) | US20140080180A1 (es) |
| EP (1) | EP2831261B1 (es) |
| JP (4) | JP6225162B2 (es) |
| KR (2) | KR20200037425A (es) |
| CN (2) | CN104350156B (es) |
| AR (2) | AR090340A1 (es) |
| AU (3) | AU2013240280A1 (es) |
| BR (1) | BR112014023176B1 (es) |
| CA (2) | CA3086730A1 (es) |
| ES (1) | ES2654424T3 (es) |
| HR (1) | HRP20171966T1 (es) |
| IL (1) | IL234377B (es) |
| MX (3) | MX365947B (es) |
| MY (1) | MY171729A (es) |
| PL (1) | PL2831261T3 (es) |
| RU (2) | RU2017139267A (es) |
| SG (4) | SG11201406112TA (es) |
| SI (1) | SI2831261T1 (es) |
| WO (1) | WO2013148249A1 (es) |
| ZA (1) | ZA201406434B (es) |
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| JP2019517272A (ja) * | 2016-06-10 | 2019-06-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | タンパク質の安定化方法 |
| CA3041533A1 (en) * | 2016-11-07 | 2018-05-11 | Seattle Genetics, Inc. | Distribution of engineered-cysteine caps |
| WO2019191133A1 (en) * | 2018-03-27 | 2019-10-03 | Bristol-Myers Squibb Company | Real-time monitoring of protein concentration using ultraviolet signal |
| AU2020208395A1 (en) * | 2019-01-16 | 2021-05-20 | Regeneron Pharmaceuticals, Inc. | Methods for characterizing disulfide bonds |
| CN109725088B (zh) * | 2019-03-16 | 2021-05-18 | 丁立平 | 一种测定豆芽中2-萘甲酸及其衍生物的气相色谱-质谱联用法 |
| CN110305903B (zh) * | 2019-07-31 | 2021-10-01 | 江苏璟泽生物医药有限公司 | 重组人促卵泡激素及其制备方法 |
| CN113155776A (zh) * | 2021-04-29 | 2021-07-23 | 华东交通大学 | 一种柑桔最佳采收期预测方法 |
| FR3146146A1 (fr) | 2023-02-24 | 2024-08-30 | Bgene Genetics | Production d’acide 1-4-dihydroxy-2-naphtoïque par Escherichia coli |
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2013
- 2013-03-14 RU RU2017139267A patent/RU2017139267A/ru not_active Application Discontinuation
- 2013-03-14 SG SG11201406112TA patent/SG11201406112TA/en unknown
- 2013-03-14 JP JP2015503300A patent/JP6225162B2/ja active Active
- 2013-03-14 RU RU2014137554A patent/RU2636353C2/ru active
- 2013-03-14 CN CN201380027623.1A patent/CN104350156B/zh active Active
- 2013-03-14 MX MX2014011558A patent/MX365947B/es active IP Right Grant
- 2013-03-14 SI SI201330894T patent/SI2831261T1/en unknown
- 2013-03-14 WO PCT/US2013/031383 patent/WO2013148249A1/en not_active Ceased
- 2013-03-14 CN CN201910197577.9A patent/CN109880867A/zh active Pending
- 2013-03-14 ES ES13713295.7T patent/ES2654424T3/es active Active
- 2013-03-14 CA CA3086730A patent/CA3086730A1/en not_active Abandoned
- 2013-03-14 EP EP13713295.7A patent/EP2831261B1/en active Active
- 2013-03-14 CA CA2866753A patent/CA2866753C/en active Active
- 2013-03-14 SG SG10201508420VA patent/SG10201508420VA/en unknown
- 2013-03-14 KR KR1020207008828A patent/KR20200037425A/ko not_active Ceased
- 2013-03-14 BR BR112014023176-1A patent/BR112014023176B1/pt active IP Right Grant
- 2013-03-14 HR HRP20171966TT patent/HRP20171966T1/hr unknown
- 2013-03-14 US US13/826,166 patent/US20140080180A1/en not_active Abandoned
- 2013-03-14 SG SG10202007206WA patent/SG10202007206WA/en unknown
- 2013-03-14 MY MYPI2014002775A patent/MY171729A/en unknown
- 2013-03-14 SG SG10201912877SA patent/SG10201912877SA/en unknown
- 2013-03-14 AU AU2013240280A patent/AU2013240280A1/en not_active Abandoned
- 2013-03-14 AR ARP130100828A patent/AR090340A1/es active IP Right Grant
- 2013-03-14 PL PL13713295T patent/PL2831261T3/pl unknown
- 2013-03-14 KR KR1020147026772A patent/KR102096678B1/ko active Active
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2014
- 2014-08-31 IL IL234377A patent/IL234377B/en active IP Right Grant
- 2014-09-02 ZA ZA2014/06434A patent/ZA201406434B/en unknown
- 2014-09-25 MX MX2019007032A patent/MX2019007032A/es unknown
- 2014-09-25 MX MX2018007677A patent/MX2018007677A/es unknown
- 2014-09-26 US US14/497,964 patent/US9765379B2/en active Active
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2015
- 2015-06-09 US US14/734,848 patent/US20160130624A1/en not_active Abandoned
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2017
- 2017-04-13 AU AU2017202484A patent/AU2017202484B2/en active Active
- 2017-10-05 JP JP2017195016A patent/JP2018046825A/ja active Pending
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2018
- 2018-02-28 US US15/908,526 patent/US20180291411A1/en not_active Abandoned
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2019
- 2019-02-07 US US16/270,494 patent/US20200199639A1/en not_active Abandoned
- 2019-03-18 JP JP2019049717A patent/JP2019141049A/ja not_active Withdrawn
- 2019-09-05 AU AU2019226192A patent/AU2019226192A1/en not_active Abandoned
- 2019-10-09 AR ARP190102873A patent/AR116615A2/es unknown
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2020
- 2020-08-14 JP JP2020137053A patent/JP2020202849A/ja not_active Withdrawn
- 2020-10-09 US US17/067,059 patent/US20210171997A1/en not_active Abandoned
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2022
- 2022-07-13 US US17/864,255 patent/US20230069966A1/en not_active Abandoned
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2024
- 2024-01-22 US US18/419,368 patent/US20240158825A1/en not_active Abandoned
- 2024-12-20 US US18/990,600 patent/US20250137027A1/en active Pending
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