[go: up one dir, main page]

AR114914A1 - P-hidroxifenilpiruvato dioxigenasa mutante, ácido nucleico que la codifica y su uso - Google Patents

P-hidroxifenilpiruvato dioxigenasa mutante, ácido nucleico que la codifica y su uso

Info

Publication number
AR114914A1
AR114914A1 ARP190101509A ARP190101509A AR114914A1 AR 114914 A1 AR114914 A1 AR 114914A1 AR P190101509 A ARP190101509 A AR P190101509A AR P190101509 A ARP190101509 A AR P190101509A AR 114914 A1 AR114914 A1 AR 114914A1
Authority
AR
Argentina
Prior art keywords
hppd
hydroxyphenylpyruvate
nucleic acid
protein
mutant
Prior art date
Application number
ARP190101509A
Other languages
English (en)
Inventor
Chao Song
Guizhi Liu
Bo Chen
Dehui Ding
Junjie Zhang
Zhenguo Li
Guangdi Yuan
Huarong Li
Sudong Mo
Lei Lian
Lei Wang
Original Assignee
Qingdao Kingagroot Chemical Compounds Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Kingagroot Chemical Compounds Co Ltd filed Critical Qingdao Kingagroot Chemical Compounds Co Ltd
Publication of AR114914A1 publication Critical patent/AR114914A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11027—4-Hydroxyphenylpyruvate dioxygenase (1.13.11.27)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente se refiere a una proteína p-hidroxifenilpiruvato dioxigenasa (HPPD) mutante o un fragmento bioactivo de la misma y un polinucleótido aislado que comprende una secuencia de ácido nucleico que codifica la proteína o fragmento de la misma, en donde la proteína p-hidroxifenilpiruvato dioxigenasa (HPPD) o un fragmento bioactivo de la misma conserva o mejora la propiedad de catalizar la conversión de p-hidroxifenilpiruvato (HPP) en homogentizado y es significativamente menos sensible a los herbicidas inhibidores de HPPD que una HPPD de tipo salvaje. La presente también se refiere a un constructo de ácido nucleico, un vector de expresión y una célula huésped que comprende el polinucleótido, así como a un método para producir una planta que tiene la propiedad de catalizar la conversión de p-hidroxifenilpiruvato (HPP) a homogentizado y sensibilidad significativamente reducida a los herbicidas inhibidores de HPPD.
ARP190101509A 2018-06-04 2019-06-04 P-hidroxifenilpiruvato dioxigenasa mutante, ácido nucleico que la codifica y su uso AR114914A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810565916 2018-06-04

Publications (1)

Publication Number Publication Date
AR114914A1 true AR114914A1 (es) 2020-10-28

Family

ID=68921172

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190101509A AR114914A1 (es) 2018-06-04 2019-06-04 P-hidroxifenilpiruvato dioxigenasa mutante, ácido nucleico que la codifica y su uso

Country Status (13)

Country Link
US (1) US12428627B2 (es)
EP (1) EP3805377A4 (es)
JP (1) JP7785536B2 (es)
CN (2) CN110616203B (es)
AR (1) AR114914A1 (es)
AU (2) AU2019280480B2 (es)
BR (1) BR112020024811A2 (es)
CA (1) CA3103851A1 (es)
CL (1) CL2020003180A1 (es)
MX (3) MX2020013127A (es)
MY (1) MY200994A (es)
UA (1) UA129619C2 (es)
ZA (1) ZA202007810B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113423826B (zh) * 2020-01-21 2022-09-23 山东舜丰生物科技有限公司 抗除草剂多肽、核酸及其应用
CN113249344B (zh) * 2020-02-07 2023-05-30 山东舜丰生物科技有限公司 抗除草剂突变蛋白、核酸及其应用
CN113249343B (zh) * 2020-02-07 2024-04-26 山东舜丰生物科技有限公司 具有抗除草剂特性的多肽、核酸及其应用
CN113249345B (zh) * 2020-02-07 2024-05-03 山东舜丰生物科技有限公司 抗除草剂多肽、核酸及其应用
WO2022127894A1 (zh) * 2020-12-17 2022-06-23 上海蓝十字医学科学研究所 除草剂抗性植物
CN112674303A (zh) * 2021-01-08 2021-04-20 贵州山珍宝绿色科技开发有限公司 一种石斛刺梨复合果粉的生产方法
CN118879649A (zh) * 2021-01-15 2024-11-01 江苏省农业科学院 具有除草剂抗性的hppd突变型蛋白及其应用
CN114774377B (zh) * 2021-03-24 2024-01-12 华中师范大学 Hppd蛋白、基因、载体、细胞、组合物及其应用、提高作物除草剂抗性的方法
CN113265401B (zh) * 2021-04-15 2022-06-03 江苏里下河地区农业科学研究所 一种通过基因编辑提高水稻对HPPD抑制剂类除草剂抗性的方法及其专用sgRNA
PE20242301A1 (es) * 2021-05-02 2024-12-11 Qingdao Kingagroot Chemical Compound Co Ltd Metodo para generar un nuevo gen en un organismo y su uso campo tecnico
CN116064430B (zh) * 2021-09-14 2023-08-11 山东舜丰生物科技有限公司 突变的hppd多肽及其应用
CA3236161A1 (en) * 2021-12-15 2023-06-22 Beijing Dabeinong Biotechnology Co., Ltd. Mutated hydroxyphenylpyruvate dioxygenase polypeptide, and coding gene and use thereof
CN116376854A (zh) * 2022-05-07 2023-07-04 山东舜丰生物科技有限公司 一种突变的hppd多肽及其应用
CN116162643B (zh) * 2022-11-04 2025-12-09 南京师范大学 一种用于合成高龙胆酸的重组菌株及其构建方法和应用
CN118562875B (zh) * 2024-07-31 2024-10-29 中国农业大学 RhTIP1;1蛋白及其编码基因在月季花瓣脱落/活性氧积累调控中的应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020144A2 (en) 1996-11-07 1998-05-14 Zeneca Limited Herbicide resistant plants
FR2770854B1 (fr) * 1997-11-07 2001-11-30 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un tel gene, tolerantes aux herbicides
FR2815969B1 (fr) * 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
AU1415802A (en) 2000-12-07 2002-06-18 Syngenta Ltd Herbicide resistant plants
US8216975B2 (en) 2004-04-29 2012-07-10 Syngenta Crop Protection Inc Method of labelling soya varieties
EP2099785B1 (en) * 2006-12-01 2010-05-19 Syngeta Participations AG N-cyanoalkylanthranilamides as insecticides
EP2268815B1 (en) * 2008-04-14 2016-11-23 Bayer CropScience NV Mutated hydroxyphenylpyruvate dioxygenase, dna sequence and isolation of plants which are tolerant to hppd inhibitor herbicides
EP2566968A4 (en) * 2010-05-04 2014-02-12 Basf Se PLANTS WITH INCREASED TOLERANCE TO HERBICIDES
CN103237894A (zh) 2010-08-13 2013-08-07 先锋国际良种公司 包含具有羟基苯丙酮酸双加氧酶(hppd)活性的序列的组合物和方法
EP2669373B1 (en) 2010-11-10 2016-06-01 Bayer CropScience AG HPPD variants and methods of use
US9370149B2 (en) * 2011-07-22 2016-06-21 Ricetec Aktiengesellschaft Methods and compositions to produce rice resistant to accase inhibitors
US20140059721A1 (en) * 2012-08-24 2014-02-27 Ricetec Aktiengesellschaft Rice resistant/tolerant to hppd inhibiting herbicides
EP2773764A4 (en) 2011-11-02 2015-11-11 Basf Se PLANTS WITH INCREASED TOLERANCE TO HERBICIDES
RU2636037C2 (ru) 2012-02-01 2017-11-17 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Устойчивые к глифосату растения и способы, связанные с ними
EP2992090A4 (en) * 2013-04-30 2017-01-11 Basf Se Plants having increased tolerance to herbicides
BR112016020709A2 (pt) * 2014-03-11 2017-10-10 Bayer Cropscience Ag variantes de hppd e métodos de uso
WO2015168667A2 (en) 2014-05-02 2015-11-05 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity
KR102127418B1 (ko) 2015-08-14 2020-06-26 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 부위-특이적인 뉴클레오티드 치환을 통해 글리포세이트-내성 벼를 수득하는 방법
CN105595163A (zh) * 2015-12-17 2016-05-25 吴志大 一种养生组合物及其应用
US11339404B2 (en) * 2016-06-16 2022-05-24 Farmhannong Co., Ltd. Protoporphyrinogen oxidase variants and methods and compositions for conferring and/or enhancing herbicide tolerance using the same
CN106070244B (zh) * 2016-06-22 2019-08-20 泰安市农业科学研究院 一种含有环吡氟草酮和砜吡草唑的除草组合物
US11345924B2 (en) 2017-05-11 2022-05-31 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Creation of herbicide resistant gene and use thereof

Also Published As

Publication number Publication date
CA3103851A1 (en) 2019-12-12
US12428627B2 (en) 2025-09-30
US20210230563A1 (en) 2021-07-29
BR112020024811A2 (pt) 2021-03-02
CN113403289B (zh) 2023-05-23
JP7785536B2 (ja) 2025-12-15
MX2025000701A (es) 2025-03-07
CN113403289A (zh) 2021-09-17
CL2020003180A1 (es) 2021-04-30
JP2021526849A (ja) 2021-10-11
EP3805377A1 (en) 2021-04-14
EP3805377A4 (en) 2022-08-24
AU2019280480B2 (en) 2024-07-18
AU2024205607B2 (en) 2025-11-13
MX2025000702A (es) 2025-03-07
CN110616203B (zh) 2022-04-01
MY200994A (en) 2024-01-29
AU2024205607A1 (en) 2024-09-05
ZA202007810B (en) 2024-07-31
UA129619C2 (uk) 2025-06-18
CN110616203A (zh) 2019-12-27
AU2019280480A1 (en) 2021-01-14
MX2020013127A (es) 2021-07-21

Similar Documents

Publication Publication Date Title
AR114914A1 (es) P-hidroxifenilpiruvato dioxigenasa mutante, ácido nucleico que la codifica y su uso
AR121761A2 (es) Polipéptidos que presentan actividad de fosfolipasa y polinucleótidos que codifican los mismos
CL2017002820A1 (es) Variantes de alfa-amilasa y polinucleótidos que codifican las mismas
MX2017010721A (es) Interruptores transcripcionales activados mediante unión y métodos para su uso.
CL2009000082A1 (es) Acido nucleico que codifica anticuerpos anti cd79a o anti cd79b para el tratamiento de tumores hematopoyeticos; vector y células huésped que los comprenden; polipeptido codificado por dicho acido nucleico, inmunoconjugado con el cuerpo.
CL2011001763A1 (es) Polipeptidos con actividad hidroxifenilpiruvato dioxigenasa (hppd); polinucleotido que lo codifica; casete de expresion y vector que comprende dicho acido nucleico, celula vegetal transformada con dichas construcciones moleculares, y metodo para conferir resistencia o tolerancia a un inhibidor de hppd en una planta.
CO2018006301A2 (es) Mutantes de proteína f de rsv
AR113177A2 (es) Polipéptidos que tienen actividad de lisozima y polinucleótidos que los codifican
MX2020004145A (es) Variantes de desoxirribonucleasa (dnasa).
CL2013001717A1 (es) Molecula de acido nucleico que codifica un polipeptido con actividad enzimatica de una enzima hidroxifenilpiruvato dioxigenasa (hppd); metodo de produccion de una planta de soja resistente a un inhibidor de hppd y/o glufosinato; metodo de control de malezas; metodo para identificar el evento syht0h2 en una muestra; y kit de deteccion.
BR112017008108A2 (pt) incorporação de aminoácidos não naturais em proteínas
CO2017005842A2 (es) Anticuerpos heterodiméricos que se unen a cd3 y cd38
CO2017011238A2 (es) Anticuerpos anti-c1s humanizados
PE20151613A1 (es) Construcciones de anticuerpos para cdh19 y cd3
UY32144A (es) Mutantes de ahas resistentes a herbicidas y metodos de uso
AR105984A1 (es) Variantes de hppd (4-hidroxifenilpiruvato dioxigenasas) y métodos de uso
MX2019000743A (es) Variantes de serinproteasa y polinucleotidos que las codifican.
MX391773B (es) Variantes de lipasa y polinucleótidos que las codifican.
MX2017007105A (es) Variantes de lipasa y polinucleotidos que las codifican.
MX2017008587A (es) Mirna natural para controlar la expresion de gen y uso del mismo.
EA201990163A1 (ru) Консервация микроорганизмов
CO2018007442A2 (es) Vacunas contra el cáncer
AR108656A1 (es) Proteína de resistencia a herbicidas, gen que la codifica y uso de los mismos
MX2017010392A (es) Proteina resistente a herbicidas, gen que codifica y usa la misma.
BR112018009245A2 (pt) drimenol sintases iii