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AR105867A1 - VARIANTES DE QUIMOSINA CON PROPIEDADES MEJORADAS DE a-S1-CASEÍNA - Google Patents

VARIANTES DE QUIMOSINA CON PROPIEDADES MEJORADAS DE a-S1-CASEÍNA

Info

Publication number
AR105867A1
AR105867A1 ARP160102650A ARP160102650A AR105867A1 AR 105867 A1 AR105867 A1 AR 105867A1 AR P160102650 A ARP160102650 A AR P160102650A AR P160102650 A ARP160102650 A AR P160102650A AR 105867 A1 AR105867 A1 AR 105867A1
Authority
AR
Argentina
Prior art keywords
chymosin
polypeptide
isolated
variant
casein
Prior art date
Application number
ARP160102650A
Other languages
English (en)
Inventor
Poulsen Lone
Rasmussen Pia
Hornbaek Tina
Lykke Marvig Nielsen Cecilie
Original Assignee
Chr Hansen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chr Hansen As filed Critical Chr Hansen As
Publication of AR105867A1 publication Critical patent/AR105867A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/045—Coagulation of milk without rennet or rennet substitutes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Dairy Products (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Reivindicación 1: Una variante de polipéptido de quimosina aislada caracterizada porque: (a) la variante de polipéptido de quimosina aislada tiene un valor C/P que es al menos el 200% del valor C/P de quimosina de camello aislada caracterizada por el polipéptido maduro de SEQ ID Nº 2; y (b) la variante de polipéptido de quimosina aislada escinde la aS1-caseína con una frecuencia inferior al 80% de la frecuencia de escisión de aS1-caseína de quimosina de camello aislada caracterizada por el polipéptido maduro de SEQ ID Nº 2, en donde la escisión de aS1-caseína se determina por cuantificación de péptidos de aS1-caseína obtenidos por incubación de leche descremada con la variante de quimosina o quimosina de camello, en donde la cuantificación se lleva a cabo por RP-HPLC acoplada con un espectrómetro de masa ESI-Q-TOF. Reivindicación 9: Un método para preparar una variante de polipéptido de quimosina aislada de acuerdo con cualquiera de las reivindicaciones 1 a 4 que comprende las siguientes etapas: (a): preparación de una alteración en una o varias posiciones en la secuencia de ADN que codifica el polipéptido maduro de SEQ ID Nº 2, en donde la alteración comprende una sustitución, una deleción o una inserción en al menos una posición de aminoácido; (b): producción y aislamiento del polipéptido alterado de la etapa (a). Reivindicación 16: Uso de una variante de polipéptido de quimosina de acuerdo con la reivindicación 15 en un proceso para preparar queso de pasta hilada, Cheddar, de tipo continental, queso blando o queso blanco salado.
ARP160102650A 2015-08-31 2016-08-31 VARIANTES DE QUIMOSINA CON PROPIEDADES MEJORADAS DE a-S1-CASEÍNA AR105867A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15183205 2015-08-31

Publications (1)

Publication Number Publication Date
AR105867A1 true AR105867A1 (es) 2017-11-15

Family

ID=54014672

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160102650A AR105867A1 (es) 2015-08-31 2016-08-31 VARIANTES DE QUIMOSINA CON PROPIEDADES MEJORADAS DE a-S1-CASEÍNA

Country Status (12)

Country Link
US (1) US10941389B2 (es)
EP (1) EP3344049A1 (es)
JP (1) JP2018525996A (es)
KR (1) KR102699499B1 (es)
CN (1) CN108135193A (es)
AR (1) AR105867A1 (es)
AU (1) AU2016315269B2 (es)
BR (1) BR112018003493A2 (es)
CA (1) CA2995755A1 (es)
MX (1) MX2018002366A (es)
WO (1) WO2017037092A1 (es)
ZA (1) ZA201801030B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014029012B1 (pt) 2012-05-25 2022-05-03 Chr. Hansen A/S Métodos para a produção de uma variante de polipeptídeo de quimosina isolada, e para fabricar um alimento ou produto alimentício à base de leite
BR112016017502B1 (pt) 2014-02-26 2023-10-24 Chr. Hansen A/S Variantes de polipeptídeo de quimosina isoladas método para a produção das mesmas e método para a fabricação de um produto de alimentação humana ou animal
RU2740317C2 (ru) 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
WO2017037092A1 (en) 2015-08-31 2017-03-09 Chr. Hansen A/S Variants of chymosin with improved properties
CN109688825A (zh) * 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体
MX389656B (es) * 2016-05-19 2025-03-20 Chr Hansen As Variantes de quimosina con propiedades mejoradas de coagulación de leche.
EP4504929A2 (en) 2022-04-06 2025-02-12 Chr. Hansen A/S Aspartic protease, methods and uses thereof
WO2025099224A1 (en) * 2023-11-08 2025-05-15 Dsm Ip Assets B.V. Improved enzyme variants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO841276L (no) 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant dna-koding for et polypeptid som utviser melkesammenloepende aktivitet
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
WO2002036752A2 (en) 2000-11-06 2002-05-10 Chr. Hansen A/S Method of producing non-bovine chymosin and use thereof
SG188669A1 (en) 2002-10-02 2013-04-30 Catalyst Biosciences Inc Method of generating and screening for proteases with altered specificity
BRPI0412007A (pt) 2003-06-27 2006-08-15 Bioren Inc mutagênese look-through
US7482148B2 (en) 2004-03-30 2009-01-27 Sudershan Biotech Ltd. Recombinant calf-chymosin and a process for producing the same
EP3763220A1 (en) 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
RU2495931C2 (ru) 2009-03-24 2013-10-20 Меито Санге Ко., Лтд. Полученная из микроорганизма протеаза усовершенствованного типа, обеспечивающая свертывание молока
EP3450552A3 (en) * 2012-05-03 2019-05-08 DSM IP Assets B.V. Improved enzyme variants
BR112014029012B1 (pt) * 2012-05-25 2022-05-03 Chr. Hansen A/S Métodos para a produção de uma variante de polipeptídeo de quimosina isolada, e para fabricar um alimento ou produto alimentício à base de leite
CN103484488B (zh) * 2013-09-16 2015-07-15 中国农业科学院生物技术研究所 优化的牛凝乳酶原基因及其分泌表达方法和应用
BR112016017502B1 (pt) 2014-02-26 2023-10-24 Chr. Hansen A/S Variantes de polipeptídeo de quimosina isoladas método para a produção das mesmas e método para a fabricação de um produto de alimentação humana ou animal
CA2976358A1 (en) 2015-02-10 2016-08-18 Chr. Hansen A/S Blends of chymosins with improved milk-clotting properties
RU2740317C2 (ru) * 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
WO2017037092A1 (en) 2015-08-31 2017-03-09 Chr. Hansen A/S Variants of chymosin with improved properties

Also Published As

Publication number Publication date
KR102699499B1 (ko) 2024-08-30
RU2018109375A3 (es) 2020-03-02
JP2018525996A (ja) 2018-09-13
WO2017037092A1 (en) 2017-03-09
US20180251747A1 (en) 2018-09-06
CN108135193A (zh) 2018-06-08
ZA201801030B (en) 2018-12-19
BR112018003493A2 (pt) 2018-09-18
CA2995755A1 (en) 2017-03-09
KR20180038569A (ko) 2018-04-16
RU2018109375A (ru) 2019-10-03
US10941389B2 (en) 2021-03-09
RU2764544C2 (ru) 2022-01-18
MX2018002366A (es) 2018-04-11
AU2016315269A1 (en) 2018-03-08
AU2016315269B2 (en) 2021-09-23
EP3344049A1 (en) 2018-07-11

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