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AR105620A1 - Variantes de quimosina - Google Patents

Variantes de quimosina

Info

Publication number
AR105620A1
AR105620A1 ARP160101858A ARP160101858A AR105620A1 AR 105620 A1 AR105620 A1 AR 105620A1 AR P160101858 A ARP160101858 A AR P160101858A AR P160101858 A ARP160101858 A AR P160101858A AR 105620 A1 AR105620 A1 AR 105620A1
Authority
AR
Argentina
Prior art keywords
isolated
casein
chymosin polypeptide
chymosin
polypeptide variant
Prior art date
Application number
ARP160101858A
Other languages
English (en)
Inventor
Fodor Hansen Enikoe
Lund Martin
Jaeckel Christian
Maarten Van Den Brink Johannes
Jeppesen Iben
Riisberg Lone
Original Assignee
Chr Hansen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chr Hansen As filed Critical Chr Hansen As
Publication of AR105620A1 publication Critical patent/AR105620A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/061—Addition of, or treatment with, microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Dairy Products (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Cosmetics (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Asimismo se provee un método para producir una variante de polipéptido quimosina y un método para producir un producto alimenticio a base de leche. Reivindicación 1: Una variante de polipéptido de quimosina aislada, caracterizada porque: (a) la variante de polipéptido de quimosina aislada tiene una actividad coagulante específica (IMCU/mg de proteína total) que es al menos un 70% de la actividad coagulante específica del polipéptido de quimosina de camello aislada caracterizado por la SEQ ID Nº 4; y (b) la variante de polipéptido de quimosina aislada escinde la b-caseína con una frecuencia inferior al 50% de la frecuencia de la escisión de b-caseína del polipéptido de quimosina aislada caracterizado por la SEQ ID Nº 4, donde la escisión de b-caseína se determina cuantificando los péptidos de b-caseína obtenidos por incubación de leche descremada con la variante de quimosina o la quimosina de camello, donde la cuantificación se lleva a cabo mediante RP-HPLC acoplada a un espectrómetro de masa ESI-Q-TOF.
ARP160101858A 2015-06-22 2016-06-22 Variantes de quimosina AR105620A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15173099 2015-06-22

Publications (1)

Publication Number Publication Date
AR105620A1 true AR105620A1 (es) 2017-10-25

Family

ID=53496438

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160101858A AR105620A1 (es) 2015-06-22 2016-06-22 Variantes de quimosina

Country Status (12)

Country Link
US (1) US11174473B2 (es)
EP (2) EP3310914B1 (es)
JP (1) JP6856551B2 (es)
KR (1) KR102612929B1 (es)
CN (1) CN107849550B (es)
AR (1) AR105620A1 (es)
AU (1) AU2016282825B2 (es)
CA (1) CA2989488A1 (es)
DK (1) DK3310914T3 (es)
MX (1) MX2017016623A (es)
RU (1) RU2740317C2 (es)
WO (1) WO2016207214A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3929288A1 (en) 2012-05-25 2021-12-29 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
EP3110948B1 (en) 2014-02-26 2025-04-09 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
DK3310914T3 (da) 2015-06-22 2022-06-20 Chr Hansen As Chymosinvarianter med forbedrede egenskaber
CA2995755A1 (en) * 2015-08-31 2017-03-09 Chr. Hansen A/S Variants of chymosin with improved properties
BR112018073261B1 (pt) * 2016-05-19 2023-04-25 Chr. Hansen A/S Variante de polipeptídeo quimosina isolada, seu método de produção e seu uso, método para produção de um alimento ou produto alimentício
WO2017198829A1 (en) * 2016-05-19 2017-11-23 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
EP4373285A1 (en) 2021-07-20 2024-05-29 Chr. Hansen A/S Composition for clotting milk, methods and uses thereof
CA3245506A1 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S ASPARTIC PROTEASE, PROCESSES AND ASSOCIATED USES

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549390A (en) * 1968-09-09 1970-12-22 Miles Lab Milk-clotting enzyme product and process therefor
EP0123928A3 (en) 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
AR031620A1 (es) 2000-11-06 2003-09-24 Eidgenoess Tech Hochschule Un metodo para producir quimosina no-bovina y su uso
RU2296160C2 (ru) * 2001-02-23 2007-03-27 ДСМ Ай Пи ЭССЕТС Б.В. Новые гены, кодирующие новые протеолитические ферменты
CN104630192A (zh) 2002-10-02 2015-05-20 催化剂生物科学有限公司 制备与筛选改变特异性蛋白酶的方法
BRPI0412007A (pt) 2003-06-27 2006-08-15 Bioren Inc mutagênese look-through
WO2005094185A2 (en) 2004-03-30 2005-10-13 Sudershan Biotech Limited Recombinant calf-chymosin and a process for producing the same
EP3763220A1 (en) 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
ES2445710T3 (es) 2009-03-24 2014-03-04 Meito Sangyo Co., Ltd. Proteasa coagulante de la leche de tipo mejorado derivada de un microorganismo
HK1207881A1 (en) * 2012-05-03 2016-02-12 帝斯曼知识产权资产管理有限公司 Improved enzyme variants
EP3929288A1 (en) * 2012-05-25 2021-12-29 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
KR20150062717A (ko) * 2013-11-29 2015-06-08 롯데푸드 주식회사 발효 크림치즈 제조방법
EP3110948B1 (en) * 2014-02-26 2025-04-09 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
UA125925C2 (uk) 2015-02-10 2022-07-13 Кр. Гансен А/С Композиція для згортання молока, що містить суміш хімозинів, харчова та кормова композиції, що містять суміш хімозинів, застосування композиції для згортання молока, спосіб одержання продукту на основі молока та спосіб одержання ферментованого молочного продукту з використанням композиції для згортання молока
DK3310914T3 (da) 2015-06-22 2022-06-20 Chr Hansen As Chymosinvarianter med forbedrede egenskaber
CA2995755A1 (en) 2015-08-31 2017-03-09 Chr. Hansen A/S Variants of chymosin with improved properties
BR112018073261B1 (pt) * 2016-05-19 2023-04-25 Chr. Hansen A/S Variante de polipeptídeo quimosina isolada, seu método de produção e seu uso, método para produção de um alimento ou produto alimentício
WO2017198829A1 (en) * 2016-05-19 2017-11-23 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Also Published As

Publication number Publication date
US20180187179A1 (en) 2018-07-05
JP2018525974A (ja) 2018-09-13
MX2017016623A (es) 2018-05-15
EP4079849A1 (en) 2022-10-26
KR20180021098A (ko) 2018-02-28
BR112017027536A2 (pt) 2018-09-11
AU2016282825B2 (en) 2022-11-17
CA2989488A1 (en) 2016-12-29
EP3310914A1 (en) 2018-04-25
AU2016282825A1 (en) 2018-01-04
NZ738238A (en) 2024-07-05
US11174473B2 (en) 2021-11-16
CN107849550B (zh) 2021-09-03
CN107849550A (zh) 2018-03-27
WO2016207214A1 (en) 2016-12-29
RU2017144572A (ru) 2019-07-22
DK3310914T3 (da) 2022-06-20
JP6856551B2 (ja) 2021-04-07
KR102612929B1 (ko) 2023-12-12
RU2017144572A3 (es) 2020-01-27
RU2740317C2 (ru) 2021-01-13
EP3310914B1 (en) 2022-04-06

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