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MX2013002249A - Acidos nucleicos, composiciones y metodos para la escision de acidos nucleicos objetivos. - Google Patents

Acidos nucleicos, composiciones y metodos para la escision de acidos nucleicos objetivos.

Info

Publication number
MX2013002249A
MX2013002249A MX2013002249A MX2013002249A MX2013002249A MX 2013002249 A MX2013002249 A MX 2013002249A MX 2013002249 A MX2013002249 A MX 2013002249A MX 2013002249 A MX2013002249 A MX 2013002249A MX 2013002249 A MX2013002249 A MX 2013002249A
Authority
MX
Mexico
Prior art keywords
nucleic acids
nucleic acid
excision
compositions
methods
Prior art date
Application number
MX2013002249A
Other languages
English (en)
Inventor
Kirsten R Benjamin
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of MX2013002249A publication Critical patent/MX2013002249A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Aquí se proveen ácidos nucleicos, composiciones y métodos que permiten la escisión de uno o más loci del genoma de una célula hospedera. En particular, aquí se provee un constructo de ácido nucleico excindible que comprende, en una orientación 5' a 3': un primer ácido nucleico de repetición en tándem, un primer sitio de reconocimiento de endonucleasa de inserción alélica, un ácido nucleico objetivo, un segundo sitio de reconocimiento de endonucleasa de inserción alélica, y un segundo ácido nucleico de repetición en tándem. En algunas modalidades, el constructo de ácido nucleico excindible es integrado en el genoma de la célula hospedera, y el ácido nucleico objetivo puede ser escindido del genoma de la célula hospedera al poner en contacto los sitios de reconocimiento de endonucleasa de inserción alélica con una o más endonucleasas de inserción alélica apropiadas.
MX2013002249A 2010-08-30 2011-08-29 Acidos nucleicos, composiciones y metodos para la escision de acidos nucleicos objetivos. MX2013002249A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37835010P 2010-08-30 2010-08-30
PCT/US2011/049615 WO2012030747A1 (en) 2010-08-30 2011-08-29 Nucleic acids, compositions and methods for the excision of target nucleic acids

Publications (1)

Publication Number Publication Date
MX2013002249A true MX2013002249A (es) 2013-05-30

Family

ID=43805849

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013002249A MX2013002249A (es) 2010-08-30 2011-08-29 Acidos nucleicos, composiciones y metodos para la escision de acidos nucleicos objetivos.

Country Status (12)

Country Link
US (2) US7919605B1 (es)
EP (2) EP2611923B1 (es)
JP (1) JP5883449B2 (es)
KR (1) KR20130100127A (es)
CN (1) CN103228789B (es)
AU (1) AU2011296245B2 (es)
CA (1) CA2809645A1 (es)
DK (1) DK2611923T3 (es)
MX (1) MX2013002249A (es)
SG (1) SG188251A1 (es)
WO (1) WO2012030747A1 (es)
ZA (1) ZA201301177B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7919605B1 (en) 2010-08-30 2011-04-05 Amyris, Inc. Nucleic acids, compositions and methods for the excision of target nucleic acids
CN103492578B (zh) * 2011-04-27 2016-10-19 阿迈瑞斯公司 用于基因组修饰的方法
NL2011912C2 (en) 2013-12-06 2015-06-09 Univ Delft Tech Novel genome alteration system for microorganisms.
CN106029886B (zh) * 2013-12-19 2021-02-05 阿迈瑞斯公司 基因组整合的方法
NL2012608B1 (en) 2014-04-11 2016-05-09 Heineken Supply Chain Bv Ureohydrolases as dominant selectable markers in yeast.
WO2017201311A2 (en) 2016-05-18 2017-11-23 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
WO2018025206A1 (en) * 2016-08-02 2018-02-08 Kyoto University Method for genome editing
GB201805676D0 (en) * 2018-04-05 2018-05-23 Imperial Innovations Ltd Compositions
JP7517002B2 (ja) * 2020-09-10 2024-07-17 トヨタ自動車株式会社 形質転換体の製造方法及び形質転換方法
CN112239765A (zh) * 2020-10-15 2021-01-19 天津科技大学 酵母定点双dsb同步诱导模型的构建方法
JP2022087881A (ja) * 2020-12-02 2022-06-14 トヨタ自動車株式会社 形質転換用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法
JP2022099419A (ja) * 2020-12-23 2022-07-05 トヨタ自動車株式会社 形質転換用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
DE10131786A1 (de) 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
MX2011005195A (es) 2008-11-19 2011-09-01 Amyris Inc Composiciones y metodos para el ensamblaje de polinucleotidos.
EP2403944B1 (en) * 2009-03-06 2017-06-28 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US7919605B1 (en) 2010-08-30 2011-04-05 Amyris, Inc. Nucleic acids, compositions and methods for the excision of target nucleic acids

Also Published As

Publication number Publication date
ZA201301177B (en) 2014-04-30
AU2011296245A1 (en) 2013-04-11
HK1181070A1 (en) 2013-11-01
US7919605B1 (en) 2011-04-05
JP5883449B2 (ja) 2016-03-15
CN103228789B (zh) 2015-11-25
WO2012030747A1 (en) 2012-03-08
DK2611923T3 (da) 2014-09-08
CN103228789A (zh) 2013-07-31
US20120052582A1 (en) 2012-03-01
EP2611923A1 (en) 2013-07-10
AU2011296245B2 (en) 2015-01-15
US9018364B2 (en) 2015-04-28
JP2013537799A (ja) 2013-10-07
EP2611923B1 (en) 2014-07-02
EP2765199A1 (en) 2014-08-13
SG188251A1 (en) 2013-04-30
CA2809645A1 (en) 2012-03-08
KR20130100127A (ko) 2013-09-09

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