AR086406A1 - Plantas transgenicas resistentes a nematodos - Google Patents
Plantas transgenicas resistentes a nematodosInfo
- Publication number
- AR086406A1 AR086406A1 ARP120101716A ARP120101716A AR086406A1 AR 086406 A1 AR086406 A1 AR 086406A1 AR P120101716 A ARP120101716 A AR P120101716A AR P120101716 A ARP120101716 A AR P120101716A AR 086406 A1 AR086406 A1 AR 086406A1
- Authority
- AR
- Argentina
- Prior art keywords
- polynucleotide
- chain
- plant
- expression
- seq
- Prior art date
Links
- 241000244206 Nematoda Species 0.000 title abstract 2
- 230000009261 transgenic effect Effects 0.000 title abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 6
- 102000040430 polynucleotide Human genes 0.000 abstract 6
- 239000002157 polynucleotide Substances 0.000 abstract 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 3
- 102100025535 Delta(14)-sterol reductase TM7SF2 Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 108700001094 Plant Genes Proteins 0.000 abstract 1
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 abstract 1
- 229930182558 Sterol Natural products 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 108010042764 delta(14)-sterol reductase Proteins 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000009368 gene silencing by RNA Effects 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000003071 parasitic effect Effects 0.000 abstract 1
- 235000002378 plant sterols Nutrition 0.000 abstract 1
- 235000003702 sterols Nutrition 0.000 abstract 1
- 150000003432 sterols Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
- C12Y103/0107—DELTA14-sterol reductase (1.3.1.70)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Composiciones de ARN bicatenarios y plantas transgénicas capaces de inhibir la expresión de genes de plantas y métodos asociados con ello. Específicamente, uso de interferencia de ARN para inhibir la expresión de un polinucleótido de esterol C-14 reductasa de planta, y generación de plantas que tienen una mayor resistencia a nematodos parásitos.Reivindicación 1: Un vector de expresión aislado caracterizado porque codifica un ARN bicatenario que comprende una primera cadena y una segunda cadena complementaria a la primera cadena, en donde la primera cadena es sustancialmente idéntica a al menos 19, 20 ó 21 nucleótidos consecutivos de un polinucleótido blanco de planta que codifica una esterol C-14 reductasa de planta seleccionada del grupo que consiste en (a) un polinucleótido que tiene una secuencia establecida en la SEQ ID Nº 1, (b) un polinucleótido que tiene una secuencia establecida en la SEQ ID Nº 4, y (c) un polinucleótido que tiene una secuencia establecida en la SEQ ID Nº 6, en donde el ARN bicatenario inhibe la expresión del polinucleótido objeto.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161486367P | 2011-05-16 | 2011-05-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR086406A1 true AR086406A1 (es) | 2013-12-11 |
Family
ID=47176365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120101716A AR086406A1 (es) | 2011-05-16 | 2012-05-15 | Plantas transgenicas resistentes a nematodos |
Country Status (2)
| Country | Link |
|---|---|
| AR (1) | AR086406A1 (es) |
| WO (1) | WO2012156902A1 (es) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3493997A (en) * | 1996-06-21 | 1998-01-07 | General Hospital Corporation, The | Plant sterol reductases and uses thereof |
| CN1247569A (zh) * | 1996-12-26 | 2000-03-15 | 孟山都公司 | 改变了甾醇生物合成途径的转基因植物 |
| CA2603151A1 (en) * | 2005-03-30 | 2006-10-05 | Jitao Zou | Identification of a sterol acyltransferase gene |
-
2012
- 2012-05-15 AR ARP120101716A patent/AR086406A1/es not_active Application Discontinuation
- 2012-05-15 WO PCT/IB2012/052410 patent/WO2012156902A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012156902A1 (en) | 2012-11-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |