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AR084162A1 - Evento 8291.45.36.2 apilado de tolerancia a herbicidas, lineas de soja transgenica relacionadas y deteccion del mismo - Google Patents

Evento 8291.45.36.2 apilado de tolerancia a herbicidas, lineas de soja transgenica relacionadas y deteccion del mismo

Info

Publication number
AR084162A1
AR084162A1 ARP110104546A ARP110104546A AR084162A1 AR 084162 A1 AR084162 A1 AR 084162A1 AR P110104546 A ARP110104546 A AR P110104546A AR P110104546 A ARP110104546 A AR P110104546A AR 084162 A1 AR084162 A1 AR 084162A1
Authority
AR
Argentina
Prior art keywords
event
detection
plant
seed
cygosity
Prior art date
Application number
ARP110104546A
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English (en)
Original Assignee
Dow Agrosciences Llc
Ms Technologies Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Ms Technologies Llc filed Critical Dow Agrosciences Llc
Publication of AR084162A1 publication Critical patent/AR084162A1/es

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
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    • C12N9/10Transferases (2.)
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    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/10923-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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    • C12Y114/11Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11017Taurine dioxygenase (1.14.11.17)
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    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01183Phosphinothricin acetyltransferase (2.3.1.183)
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    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/010193-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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  • Developmental Biology & Embryology (AREA)
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Abstract

La planta modificada por el cultivo de la semilla divulgado. Evento pDAB8291.45.36.2 de soja, que incluye un casete de expresión que comprende características múltiples que confieren resistencia a herbicidas de glifosato, ariloxialcanoato, y glufosinato. También se divulgan métodos de control de las malezas resistentes, mejora de plantas, y plantas tolerantes a herbicidas. En algunas realizaciones, dicha secuencia de evento puede estar “apilada” con otras características, con inclusión, por ejemplo, de otro(s) gen(es) de tolerancia a herbicida y/o proteínas inhibidoras de insectos. Métodos de detección, con inclusión e los ensayos de punto final de PCR TaqMan, para la detección del Evento pDAB8291.45.36.2 en sojas y material vegetal relacionado. Algunas realizaciones pueden realizar el análisis de cigosidad de alto rendimiento de material vegetal y otras realizaciones pueden utilizarse para identificar de manera distintiva la cigosidad de líneas de soja de mejora que comprenden el evento. Los kits y condiciones útiles para conducir estos ensayos también se proporcionan.Reivindicación 1: Una célula vegetal de soja transgénica que comprende un genoma que comprende un primer segmento de polinucleótido que posee al menos 95% de identidad con SEQ ID Nº 14 y un segundo segmento de polinucleótido que posee al menos 95% de identidad con SEQ ID Nº 15. Reivindicación 2: Una semilla de soja que comprende un genoma que comprende el Evento pDAB8291.45.36.2 como está presente en una semilla representativa depositada en la Colección de Cultivos Tipo Americana (ATCC) con el Acceso Nº PTA-11335.
ARP110104546A 2010-12-03 2011-12-05 Evento 8291.45.36.2 apilado de tolerancia a herbicidas, lineas de soja transgenica relacionadas y deteccion del mismo AR084162A1 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41970910P 2010-12-03 2010-12-03
US201161471845P 2011-04-05 2011-04-05
US201161511664P 2011-07-26 2011-07-26
US201161521798P 2011-08-10 2011-08-10

Publications (1)

Publication Number Publication Date
AR084162A1 true AR084162A1 (es) 2013-04-24

Family

ID=46172296

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110104546A AR084162A1 (es) 2010-12-03 2011-12-05 Evento 8291.45.36.2 apilado de tolerancia a herbicidas, lineas de soja transgenica relacionadas y deteccion del mismo

Country Status (4)

Country Link
US (1) US9540656B2 (es)
AR (1) AR084162A1 (es)
BR (1) BR112013015745B1 (es)
WO (1) WO2012075429A1 (es)

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