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AR079338A1 - Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes - Google Patents

Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes

Info

Publication number
AR079338A1
AR079338A1 ARP100104547A ARP100104547A AR079338A1 AR 079338 A1 AR079338 A1 AR 079338A1 AR P100104547 A ARP100104547 A AR P100104547A AR P100104547 A ARP100104547 A AR P100104547A AR 079338 A1 AR079338 A1 AR 079338A1
Authority
AR
Argentina
Prior art keywords
amino acid
variants
protease
occupies
seq
Prior art date
Application number
ARP100104547A
Other languages
English (en)
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of AR079338A1 publication Critical patent/AR079338A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Accessory Of Washing/Drying Machine, Commercial Washing/Drying Machine, Other Washing/Drying Machine (AREA)
  • Eyeglasses (AREA)

Abstract

La presente también provee composiciones que comprenden variantes de proteasa y métodos de uso de tales variantes en composiciones de limpieza. Reivindicacion 20: Una variante de proteasa aislada de una proteasa principal, en donde la variante de proteasa tiene actividad proteolítica y comprende una secuencia de aminoácidos que comprende una alteracion en una o varias de las posiciones de aminoácido correspondientes a las posiciones de aminoácido de SEQ ID Ns:2 seleccionadas del grupo que consiste en posiciones 24, 53, 78, 97, 101, 128 y 217, en donde la al menos una alteracion es, de modo independiente, (i) una insercion de uno o varios residuos de aminoácidos corriente arriba o corriente abajo del residuo de aminoácido que ocupa la posicion, (ii) una delecion del residuo de aminoácido que ocupa la posicion o (iii) una sustitucion del residuo de aminoácido que ocupa la posicion con un residuo de aminoácido diferente, en donde cada posicion de aminoácido se numera por correspondencia con una posicion de aminoácido en la secuencia de aminoácidos de subtilisina proteasa BPN' de Bacillus amyloliquefaciens establecida en SEQ ID Ns:2 tal como se determina por alineacion de la secuencia de aminoácidos de la variante con SEQ ID Ns:2.
ARP100104547A 2009-12-09 2010-12-09 Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes AR079338A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28512709P 2009-12-09 2009-12-09
US39237310P 2010-10-12 2010-10-12

Publications (1)

Publication Number Publication Date
AR079338A1 true AR079338A1 (es) 2012-01-18

Family

ID=44146173

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP100104547A AR079338A1 (es) 2009-12-09 2010-12-09 Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes

Country Status (12)

Country Link
US (6) US8728790B2 (es)
EP (3) EP3599279A1 (es)
JP (4) JP5863666B2 (es)
CN (2) CN105368809A (es)
AR (1) AR079338A1 (es)
AU (1) AU2010328121A1 (es)
BR (1) BR112012014082B1 (es)
CA (1) CA2782891C (es)
DK (2) DK2510094T3 (es)
ES (1) ES2746931T3 (es)
MX (2) MX356282B (es)
WO (1) WO2011072099A2 (es)

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