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AR078433A1 - Metodo de preparacion de proteinas derivadas de plantas - Google Patents

Metodo de preparacion de proteinas derivadas de plantas

Info

Publication number
AR078433A1
AR078433A1 ARP100103460A ARP100103460A AR078433A1 AR 078433 A1 AR078433 A1 AR 078433A1 AR P100103460 A ARP100103460 A AR P100103460A AR P100103460 A ARP100103460 A AR P100103460A AR 078433 A1 AR078433 A1 AR 078433A1
Authority
AR
Argentina
Prior art keywords
protein
plant
fraction
plants
derived
Prior art date
Application number
ARP100103460A
Other languages
English (en)
Inventor
Aoust Marc-Andre D
Michele Dargis
Louis-Philippe Vezina
Manon Couture
Dany Paquet
Original Assignee
Medicago Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc filed Critical Medicago Inc
Publication of AR078433A1 publication Critical patent/AR078433A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/517—Plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Water Supply & Treatment (AREA)
  • Analytical Chemistry (AREA)

Abstract

Métodos de preparacion de proteínas o supraestructuras proteicas derivadas de plantas. El método puede comprender obtener una planta, o materia vegetal, que comprende proteínas localizadas en los apoplastos, o supraestructuras proteicas, producir una fraccion de protoplastos/esferoplastos y de apoplastos a partir de la planta o materia vegetal, y recuperar la fraccion de apoplastos. La fraccion de apoplastos comprende proteínas o supraestructuras proteicas derivadas de plantas. Como alternativa, las proteínas, o supraestructuras proteicas, se pueden obtener de plantas o de una materia vegetal que comprende dichas proteínas o supraestructuras proteicas derivadas de plantas, por digestion de la materia vegetal usando una composicion de enzimas degradadoras de la pared celular para producir una fraccion digerida. La fraccion digerida se filtra para producir una fraccion filtrada, y las proteínas o supraestructuras proteicas derivadas de plantas, se recuperan de la fraccion filtrada.
ARP100103460A 2009-09-22 2010-09-22 Metodo de preparacion de proteinas derivadas de plantas AR078433A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24478609P 2009-09-22 2009-09-22

Publications (1)

Publication Number Publication Date
AR078433A1 true AR078433A1 (es) 2011-11-09

Family

ID=43795254

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP100103458A AR078432A1 (es) 2009-09-22 2010-09-22 Metodo para preparar particulas similares a virus (psv) derivadas de plantas
ARP100103460A AR078433A1 (es) 2009-09-22 2010-09-22 Metodo de preparacion de proteinas derivadas de plantas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP100103458A AR078432A1 (es) 2009-09-22 2010-09-22 Metodo para preparar particulas similares a virus (psv) derivadas de plantas

Country Status (28)

Country Link
US (2) US11826419B2 (es)
EP (3) EP2480560B1 (es)
JP (2) JP5551780B2 (es)
KR (4) KR20180026562A (es)
CN (4) CN107129973A (es)
AR (2) AR078432A1 (es)
AU (2) AU2010300033B2 (es)
BR (2) BR112012006414B1 (es)
CA (2) CA2772964C (es)
DK (3) DK3354657T3 (es)
ES (3) ES2642631T3 (es)
HR (3) HRP20171564T1 (es)
HU (3) HUE038557T2 (es)
IL (4) IL218393A0 (es)
IN (2) IN2012DN02591A (es)
MX (2) MX2012003372A (es)
MY (2) MY164704A (es)
NO (2) NO2480658T3 (es)
NZ (2) NZ598481A (es)
PH (2) PH12012500565B1 (es)
PL (3) PL2480560T3 (es)
PT (3) PT3354657T (es)
RU (2) RU2579903C2 (es)
SG (2) SG178918A1 (es)
SI (3) SI2480560T1 (es)
TR (1) TR201803560T4 (es)
WO (2) WO2011035423A1 (es)
ZA (2) ZA201202836B (es)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
AU2009202819B2 (en) 2007-07-13 2014-08-28 Aramis Biotechnologies Inc. Recombinant influenza virus-like particles (VLPs) produced in transgenic plants expressing hemagglutinin
MX392585B (es) 2009-06-24 2025-03-19 Medicago Inc Particulas similares al virus de la influenza quimericas que comprenden hemaglutinina.
JP5551780B2 (ja) 2009-09-22 2014-07-16 メディカゴ インコーポレイテッド 植物由来のタンパク質を調製する方法
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) * 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
RU2655433C2 (ru) 2011-06-13 2018-05-28 Медикаго Инк. Получение вирусоподобной частицы вируса бешенства в растениях
CN103957891B (zh) * 2011-09-23 2017-01-11 美利坚合众国由健康及人类服务部部长代表 基于流感血凝素蛋白的新颖疫苗
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
AR089440A1 (es) * 2011-12-21 2014-08-20 Apse Llc Procesos que emplean particulas similares a virus (psv) con capsides resistentes a hidrolasas
JP6406655B2 (ja) * 2012-02-22 2018-10-17 三菱商事フードテック株式会社 植物性食品素材の軟化方法、軟化製剤、軟化した植物性食品素材およびそれを用いた食品
CA2864733C (en) * 2012-03-22 2018-09-18 Fraunhofer Usa, Inc. Virus-like particles comprising a matrix protein from a plant enveloped virus and uses thereof
US10202423B2 (en) 2012-09-05 2019-02-12 Medicago Inc. of Quebec, Canada Picornavirus-like particle production in plants
US9617297B2 (en) * 2012-10-11 2017-04-11 The Regents Of The University Of California Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves
MX376138B (es) * 2013-03-28 2025-03-07 Medicago Inc Produccion de particulas similares a virus de influenza en plantas.
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
WO2015034042A1 (ja) * 2013-09-06 2015-03-12 三菱化学株式会社 植物を用いたタンパク質の製造方法
US10744199B2 (en) 2013-10-11 2020-08-18 The Usa, As Represented By The Secretary, Dept. Of Health And Human Services Epstein-Barr virus vaccines
TWI714522B (zh) 2014-01-10 2021-01-01 加拿大商苜蓿股份有限公司 Cpmv強化子元件
AU2015286165B2 (en) 2014-07-11 2021-03-11 Medicago Inc. Modifying protein production in plants
IL253187B (en) 2014-12-31 2022-07-01 Us Health Novel multivalent nanoparticle-based vaccines
JP2019129705A (ja) * 2016-03-31 2019-08-08 日本たばこ産業株式会社 植物に物質を導入する方法
IL303650B2 (en) 2016-09-02 2025-02-01 Us Health Stable group 2 trimers from the stem region of influenza hemagglutinin and their uses
MX2020005530A (es) 2017-11-30 2021-01-15 Medicago Inc Proteinas vp1 de norovirus modificadas y psv que comprenden proteinas vp1 de norovirus modificadas.
US12139511B2 (en) 2018-06-27 2024-11-12 Aramis Biotechnologies Inc. Influenza virus hemagglutinin mutants
JP7429684B6 (ja) * 2018-07-13 2024-03-15 メディカゴ インコーポレイテッド 改変ノロウイルスｖｐ１タンパク質および改変ノロウイルスｖｐ１タンパク質を含むｖｌｐ
CN109112093B (zh) * 2018-09-05 2021-11-09 上海交通大学 一种青蒿原生质体高效分离及瞬时转化的方法
AU2020262433A1 (en) * 2019-04-25 2021-12-23 Flagship Pioneering Innovations Vi, Llc Compositions and methods relating to plant messenger packs
CN110483616B (zh) * 2019-07-31 2021-06-04 中国农业科学院农产品加工研究所 从感染病原菌的植物组织中分离病原菌分泌的质外体效应蛋白的方法
CN111060696B (zh) * 2019-12-27 2023-09-08 湖南农业大学 一种降低植物小分子信号肽假阳性率的方法
CN111718394A (zh) * 2020-06-28 2020-09-29 广西壮族自治区农业科学院 一种基于bpp法的甘蔗组织变性蛋白提取方法
CN111826394A (zh) * 2020-08-07 2020-10-27 浙江华缔药业集团医药开发有限公司 植物瞬时表达载体的构建及应用
EP4351320A1 (en) 2021-06-09 2024-04-17 ImmunityBio, Inc. Methods and systems for producing a protein of interest in a plant
CN114934007B (zh) * 2022-07-01 2023-11-24 广东省农业科学院作物研究所 一种甘薯原生质体的制备方法
JP7745040B1 (ja) 2024-06-21 2025-09-26 デンカ株式会社 目的タンパク質の回収方法及び製造方法
CN120082594B (zh) * 2025-04-07 2025-10-28 贵州大学 NbClpT2在调控植物抗PVY侵染中的应用

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
WO1986003224A1 (en) 1984-11-29 1986-06-05 Scripps Clinic And Research Foundation Polypeptides and antibodies related to deglycosylated viral glycoproteins
JPS61265086A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養法
US5232833A (en) 1988-09-14 1993-08-03 Stressgen Biotechnologies Corporation Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
EP0509656B1 (en) 1991-03-28 1996-09-18 Rooperol (Na) Nv Compositions of phytosterols and phytosterolins as immunmodulators
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
US6326470B1 (en) * 1997-04-15 2001-12-04 The Penn State Research Foundation Enhancement of accessibility of cellulose by expansins
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
GB9414118D0 (en) 1994-07-13 1994-08-31 Axis Genetics Ltd Modified plant viruses as vectors of heterologous peptides
US6020169A (en) * 1995-07-20 2000-02-01 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6042832A (en) 1996-08-28 2000-03-28 Thomas Jefferson University Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins
US20010006950A1 (en) 1998-02-11 2001-07-05 Juha Punnonen Genetic vaccine vector engineering
US6489537B1 (en) 1998-08-07 2002-12-03 The Trustees Of The University Of Pennsylvania Phytochelatin synthases and uses therefor
AU759813B2 (en) 1998-08-11 2003-05-01 Kentucky Bioprocessing, Llc Method for recovering proteins from the interstitial fluid of plant tissues
US6287570B1 (en) 1998-11-23 2001-09-11 Patricia L. Foley Vaccine against swine influenza virus
JP2002533090A (ja) 1998-12-23 2002-10-08 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク 植物形質転換法
FR2791358B1 (fr) 1999-03-22 2003-05-16 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, plasmides, vecteurs, plantes et semences transgeniques les contenant et leurs methodes d'obtention
CA2370638A1 (en) 1999-04-21 2000-10-26 Igor Kardailsky Plant transformation process
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
EP1310551A4 (en) * 2000-08-08 2004-09-22 Kitasato Inst VIRUS-LIKE MICROBALLS AND METHOD FOR THEIR PRODUCTION (02/15/02)
ATE433491T1 (de) 2001-01-18 2009-06-15 Vlaams Interuniv Inst Biotech Oligomerische komplexe von chimären proteinen mit verbessertem immunogenen potential
DE60238497D1 (de) 2001-09-14 2011-01-13 Fraunhofer Ges Forschung Immunglobulin mit einem bestimmten rahmengerüst und verfahren zu dessen herstellung und verwendung
EP3009507B1 (en) 2002-02-13 2020-06-24 Wisconsin Alumini Research Foundation Signal for packaging of influenza virus vectors
AU2003219760A1 (en) 2002-02-14 2003-09-04 Novavax, Inc. Optimization of gene sequences of virus-like particles for expression in insect cells
AU2003219402B2 (en) 2002-03-19 2008-05-08 Stichting Dienst Landbouwkundig Onderzoek GnTIII (UDP-n-acethylglucosamine:beta-D mannoside beta (1,4)-N-acethylglucosaminy ltransferase III) expression in plants
BRPI0410340A (pt) 2003-05-05 2006-05-30 Dow Agrosciences Llc vetores e células para preparar composições imunoprotetoras derivadas de plantas transgênicas
BRPI0410342A (pt) 2003-05-05 2006-06-20 Dow Agrosciences Llc composições imunoprofiláticas e terapêuticas estáveis derivadas de células vegetais transgênicas e processos para produção
CA2529647C (en) 2003-06-16 2013-08-13 Medimmune Vaccines, Inc. Influenza hemagglutinin and neuraminidase variants
US8592197B2 (en) 2003-07-11 2013-11-26 Novavax, Inc. Functional influenza virus-like particles (VLPs)
CN1560227A (zh) * 2004-02-25 2005-01-05 福建师范大学 雨生红球藻原生质体制备与再生技术
WO2006016380A2 (en) 2004-08-13 2006-02-16 Council Of Scientific And Industrial Research A chimeric g protein based rabies vaccine
US9155483B2 (en) 2004-12-03 2015-10-13 The Invention Science Fund I, Llc Vision modification with reflected image
NZ583532A (en) * 2005-03-15 2011-09-30 Verenium Corp Cellulases, nucleic acids encoding them and methods for making and using them
DK2374892T3 (en) 2005-04-29 2018-03-12 Univ Cape Town Expression of viral proteins in plants
CN100410378C (zh) 2005-05-09 2008-08-13 中国农业科学院生物技术研究所 编码禽流感血凝素的基因及其植物表达载体和应用
MX2008000097A (es) * 2005-06-24 2008-03-19 Bayer Bioscience Nv Metodos para alterar la reactividad de las paredes de las celulas vegetales.
JP2009502789A (ja) 2005-07-19 2009-01-29 ダウ グローバル テクノロジーズ インコーポレイティド 組み換えインフルエンザワクチン
US7871626B2 (en) 2005-08-04 2011-01-18 St. Jude Children's Research Hospital Modified influenza virus for monitoring and improving vaccine efficiency
BRPI0617486A2 (pt) 2005-10-18 2011-07-26 Novavax Inc vacina que compreende partÍculas similares a vÍrus da gripe funcionais (vlp) e uso de uma vlp de gripe
EP2360175B1 (en) * 2005-11-22 2014-07-16 Novartis Vaccines and Diagnostics, Inc. Norovirus and Sapovirus virus-like particles (VLPs)
US8124103B2 (en) 2006-02-13 2012-02-28 Fraunhofer Usa, Inc Influenza antigens, vaccine compositions, and related methods
CN101578111A (zh) 2006-04-21 2009-11-11 美国陶氏益农公司 禽流感疫苗和使用方法
US8778353B2 (en) 2006-05-01 2014-07-15 Technovax, Inc. Influenza virus-like particle (VLP) compositions
DK2023952T3 (en) 2006-05-18 2015-10-19 Epimmune Inc Induction of immune responses to influenza virus using polypeptide and nucleic acid compositions
EP2029755A1 (en) 2006-05-22 2009-03-04 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
US20070286873A1 (en) 2006-05-23 2007-12-13 Williams John V Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor
JP5188510B2 (ja) 2007-01-17 2013-04-24 ラーナー・メディカル・デバイセズ・インコーポレイテッド 光ファイバー式の光線療法装置
US7730950B2 (en) 2007-01-19 2010-06-08 Halliburton Energy Services, Inc. Methods for treating intervals of a subterranean formation having variable permeability
PT2155880T (pt) 2007-06-15 2016-11-18 Medicago Inc Modificação da produção de glicoproteína em plantas
KR100964462B1 (ko) 2007-07-10 2010-06-16 성균관대학교산학협력단 형질전환 식물 유래의 조류독감 바이러스 백신 및 그 제조방법
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
AU2009202819B2 (en) 2007-07-13 2014-08-28 Aramis Biotechnologies Inc. Recombinant influenza virus-like particles (VLPs) produced in transgenic plants expressing hemagglutinin
RU2358981C2 (ru) * 2007-08-07 2009-06-20 Центр "Биоинженерия" Российской Академии Наук Универсальная вакцина против вируса гриппа птиц
EP2190472A2 (en) 2007-08-20 2010-06-02 Fraunhofer USA, Inc. Prophylactic and therapeutic influenza vaccines, antigens, compositions, and methods
CN101422128B (zh) * 2007-10-29 2011-06-08 中国水产科学研究院黄海水产研究所 马尾藻原生质体的分离和再生
JP2011508034A (ja) 2007-12-28 2011-03-10 ユニリーバー・ナームローゼ・ベンノートシヤープ 茶から香気成分を回収する方法
US8799801B2 (en) 2008-01-16 2014-08-05 Qualcomm Incorporated Interactive ticker
SG187500A1 (en) 2008-01-21 2013-02-28 Medicago Inc Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
JP5809054B2 (ja) 2008-07-08 2015-11-10 メディカゴ インコーポレイテッド 可溶性組換えインフルエンザ抗原
CN101626276A (zh) 2008-07-10 2010-01-13 华为技术有限公司 广告替换方法、装置及系统
CN102165062B (zh) 2008-07-18 2017-09-08 麦迪卡格公司 新的流感病毒免疫表位
JP5785080B2 (ja) 2008-08-27 2015-09-24 アリゾナ・ボード・オブ・リージェンツ・フォー・アンド・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティＡｒｉｚｏｎａ Ｂｏａｒｄ Ｏｆ Ｒｅｇｅｎｔｓ Ｆｏｒ Ａｎｄ Ｏｎ Ｂｅｈａｌｆ Ｏｆ Ａｒｉｚｏｎａ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ 植物におけるワクチン及びモノクローナル抗体治療薬の高レベル迅速生産のためのｄｎａレプリコン系
WO2010042551A2 (en) * 2008-10-06 2010-04-15 Genvault Corporation Methods for providing cellular lysates from cell wall-containing samples
WO2010077712A1 (en) 2008-12-09 2010-07-08 Novavax, Inc. Bovine respiratory syncytial virus virus-like particle (vlps)
EP2391644B1 (en) 2009-01-30 2016-04-13 System of Systems Analytics, Inc. Conformationally dynamic peptides
DK2440231T3 (da) 2009-06-10 2020-01-13 Nono Inc Co-administration af et middel bundet til et tat-internaliseringspeptid med en mastcelledegranuleringshæmmer
CA2764609C (en) 2009-06-10 2018-10-02 Alnylam Pharmaceuticals, Inc. Improved cationic lipid of formula i
MX392585B (es) 2009-06-24 2025-03-19 Medicago Inc Particulas similares al virus de la influenza quimericas que comprenden hemaglutinina.
JP5551780B2 (ja) 2009-09-22 2014-07-16 メディカゴ インコーポレイテッド 植物由来のタンパク質を調製する方法
ES2614527T3 (es) 2010-11-04 2017-05-31 Medicago Inc. Sistema de expresión en plantas
KR101847908B1 (ko) 2010-11-05 2018-04-11 노바백스, 인코포레이티드 광견병 당단백질 바이러스-유사 입자(VLPs)
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法

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