[go: up one dir, main page]

MX2012003373A - Metodos para preparar particulas tipo virus derivadas de plantas. - Google Patents

Metodos para preparar particulas tipo virus derivadas de plantas.

Info

Publication number
MX2012003373A
MX2012003373A MX2012003373A MX2012003373A MX2012003373A MX 2012003373 A MX2012003373 A MX 2012003373A MX 2012003373 A MX2012003373 A MX 2012003373A MX 2012003373 A MX2012003373 A MX 2012003373A MX 2012003373 A MX2012003373 A MX 2012003373A
Authority
MX
Mexico
Prior art keywords
plant
fraction
vlps
derived
apoplast
Prior art date
Application number
MX2012003373A
Other languages
English (en)
Inventor
Louis-Philippe Vezina
Manon Couture
Michele Dargis
Marc-Andre D Aoust
Dany Paquet
Original Assignee
Medicago Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc filed Critical Medicago Inc
Publication of MX2012003373A publication Critical patent/MX2012003373A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/517—Plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Water Supply & Treatment (AREA)
  • Analytical Chemistry (AREA)

Abstract

Se proporcionan métodos para preparar partículas tipo virus derivadas de plantas (VLPs). El método puede comprender obtener una planta, o materia de planta que comprende VLPs localizadas en el apoplasto, producir una fracción de protoplasto/esferoplasto y la fracción deñ apoplasto de la planta o materia de la planta, y recuperar la fracción del apoplasto. La fracción del apoplasto comprende VLPs derivadas de plantas. Alternativamente, las VLPs pueden obtenerse de planta o materia de planta que comprende VLPs derivadas de plantas, al digerir la materia de planta usando una composición de enzimas de degradación de pared celular para producir una fracción digerida. La fracción digerida se filtra para producir una fracción filtrada, y las VLPs derivadas de plantas se recuperan de la fracción filtrada.
MX2012003373A 2009-09-22 2010-09-21 Metodos para preparar particulas tipo virus derivadas de plantas. MX2012003373A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24478609P 2009-09-22 2009-09-22
PCT/CA2010/001488 WO2011035422A1 (en) 2009-09-22 2010-09-21 Method of preparing plant-derived vlps

Publications (1)

Publication Number Publication Date
MX2012003373A true MX2012003373A (es) 2012-05-29

Family

ID=43795254

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2012003373A MX2012003373A (es) 2009-09-22 2010-09-21 Metodos para preparar particulas tipo virus derivadas de plantas.
MX2012003372A MX2012003372A (es) 2009-09-22 2010-09-21 Metodo para preparar proteina derivada de vegetales.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2012003372A MX2012003372A (es) 2009-09-22 2010-09-21 Metodo para preparar proteina derivada de vegetales.

Country Status (28)

Country Link
US (2) US11826419B2 (es)
EP (3) EP3354657B1 (es)
JP (2) JP5551780B2 (es)
KR (4) KR20180026562A (es)
CN (4) CN107090021A (es)
AR (2) AR078432A1 (es)
AU (2) AU2010300033B2 (es)
BR (2) BR112012006414B1 (es)
CA (2) CA2772964C (es)
DK (3) DK2480560T3 (es)
ES (3) ES2911037T3 (es)
HR (3) HRP20171564T1 (es)
HU (3) HUE038557T2 (es)
IL (4) IL218393A0 (es)
IN (2) IN2012DN02637A (es)
MX (2) MX2012003373A (es)
MY (2) MY164704A (es)
NO (2) NO2480560T3 (es)
NZ (2) NZ598481A (es)
PH (2) PH12012500565B1 (es)
PL (3) PL3354657T3 (es)
PT (3) PT2480560T (es)
RU (2) RU2579903C2 (es)
SG (2) SG178918A1 (es)
SI (3) SI2480560T1 (es)
TR (1) TR201803560T4 (es)
WO (2) WO2011035423A1 (es)
ZA (2) ZA201202836B (es)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
CN101978066A (zh) 2007-11-27 2011-02-16 麦迪卡格公司 表达血凝素之转基因植物中生产的重组流感病毒样颗粒(vlp)
BRPI1015053A2 (pt) 2009-06-24 2019-07-09 Medicago Inc partículas similares a vírus da influenza quiméricas compreendendo hemaglutinina
TR201803560T4 (tr) 2009-09-22 2019-01-21 Medicago Inc Bitkiden türetilen proteinlerin hazırlanma metotları.
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
US20140227322A1 (en) * 2011-06-13 2014-08-14 Medicago Inc. Rabies virus like particle production in plants
CA2849822C (en) * 2011-09-23 2021-05-11 Gary J. Nabel, M.D. Novel influenza hemagglutinin protein-based vaccines
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
CA2850407C (en) 2011-09-30 2021-11-23 Medicago Inc. Increasing virus-like particle yield in plants
CN107164376B (zh) * 2011-12-21 2021-03-30 阿普斯有限责任公司 使用具有对水解酶抗性的衣壳的vlp的方法
JP6406655B2 (ja) * 2012-02-22 2018-10-17 三菱商事フードテック株式会社 植物性食品素材の軟化方法、軟化製剤、軟化した植物性食品素材およびそれを用いた食品
EP2827893B1 (en) * 2012-03-22 2017-11-29 Fraunhofer USA, Inc. Virus-like particles comprising a matrix protein from a plant enveloped virus and uses thereof
AU2013312971B2 (en) 2012-09-05 2019-04-18 Medicago Inc. Picornavirus-like particle production in plants
US9617297B2 (en) * 2012-10-11 2017-04-11 The Regents Of The University Of California Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves
WO2014153674A1 (en) * 2013-03-28 2014-10-02 Medicago Inc. Influenza virus-like particle production in plants
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
WO2015034042A1 (ja) 2013-09-06 2015-03-12 三菱化学株式会社 植物を用いたタンパク質の製造方法
WO2015054639A1 (en) 2013-10-11 2015-04-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Epstein-barr virus vaccines
US11441150B2 (en) 2014-01-10 2022-09-13 Medicago Inc. CPMV enhancer elements
JP6788582B2 (ja) 2014-07-11 2020-11-25 メディカゴ インコーポレイテッド 植物におけるタンパク質生産の改変
US11191727B2 (en) 2014-12-31 2021-12-07 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Multivalent nanoparticle-based vaccines
JP2019129705A (ja) * 2016-03-31 2019-08-08 日本たばこ産業株式会社 植物に物質を導入する方法
CA3035443A1 (en) 2016-09-02 2018-03-08 The Usa, As Represented By The Secretary, Dept. Of Health And Human Services Stabilized group 2 influenza hemagglutinin stem region trimers and uses thereof
BR112020010799A2 (pt) * 2017-11-30 2020-11-10 Medicago Inc. proteínas vp1 modificadas de norovírus e vlps que compreendem proteínas vp1 modificadas de norovírus
CA3103849A1 (en) 2018-06-27 2020-01-02 Medicago Inc. Influenza virus hemagglutinin mutants
US11759512B2 (en) 2018-07-13 2023-09-19 Medicago Inc. Modified norovirus VP1 proteins and VLPs comprising modified norovirus VP1 proteins
CN109112093B (zh) * 2018-09-05 2021-11-09 上海交通大学 一种青蒿原生质体高效分离及瞬时转化的方法
EP3959223A4 (en) * 2019-04-25 2023-07-19 Flagship Pioneering Innovations VI, LLC COMPOSITIONS AND METHODS FOR PLANT KNIFE PACKS
CN110483616B (zh) * 2019-07-31 2021-06-04 中国农业科学院农产品加工研究所 从感染病原菌的植物组织中分离病原菌分泌的质外体效应蛋白的方法
CN111060696B (zh) * 2019-12-27 2023-09-08 湖南农业大学 一种降低植物小分子信号肽假阳性率的方法
CN111718394A (zh) * 2020-06-28 2020-09-29 广西壮族自治区农业科学院 一种基于bpp法的甘蔗组织变性蛋白提取方法
CN111826394A (zh) * 2020-08-07 2020-10-27 浙江华缔药业集团医药开发有限公司 植物瞬时表达载体的构建及应用
EP4351320A1 (en) 2021-06-09 2024-04-17 ImmunityBio, Inc. Methods and systems for producing a protein of interest in a plant
CN114934007B (zh) * 2022-07-01 2023-11-24 广东省农业科学院作物研究所 一种甘薯原生质体的制备方法
JP7745040B1 (ja) * 2024-06-21 2025-09-26 デンカ株式会社 目的タンパク質の回収方法及び製造方法
CN120082594B (zh) * 2025-04-07 2025-10-28 贵州大学 NbClpT2在调控植物抗PVY侵染中的应用

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
WO1986003224A1 (en) 1984-11-29 1986-06-05 Scripps Clinic And Research Foundation Polypeptides and antibodies related to deglycosylated viral glycoproteins
JPS61265086A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養法
US5232833A (en) 1988-09-14 1993-08-03 Stressgen Biotechnologies Corporation Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
EP0509656B1 (en) 1991-03-28 1996-09-18 Rooperol (Na) Nv Compositions of phytosterols and phytosterolins as immunmodulators
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
US6326470B1 (en) * 1997-04-15 2001-12-04 The Penn State Research Foundation Enhancement of accessibility of cellulose by expansins
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
GB9414118D0 (en) 1994-07-13 1994-08-31 Axis Genetics Ltd Modified plant viruses as vectors of heterologous peptides
US6020169A (en) 1995-07-20 2000-02-01 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6042832A (en) 1996-08-28 2000-03-28 Thomas Jefferson University Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins
US20010006950A1 (en) 1998-02-11 2001-07-05 Juha Punnonen Genetic vaccine vector engineering
US6489537B1 (en) 1998-08-07 2002-12-03 The Trustees Of The University Of Pennsylvania Phytochelatin synthases and uses therefor
WO2000009725A2 (en) * 1998-08-11 2000-02-24 Biosource Technologies, Inc. Method for recovering proteins from the interstitial fluid of plant tissues
US6287570B1 (en) 1998-11-23 2001-09-11 Patricia L. Foley Vaccine against swine influenza virus
AU2594300A (en) 1998-12-23 2000-07-12 Samuel Roberts Noble Foundation, Inc., The Plant transformation process
FR2791358B1 (fr) 1999-03-22 2003-05-16 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, plasmides, vecteurs, plantes et semences transgeniques les contenant et leurs methodes d'obtention
NZ513993A (en) 1999-04-21 2001-09-28 Samuel Roberts Noble Found Inc Plant transformation process
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
AU2001276752A1 (en) * 2000-08-08 2002-02-18 The Kitasato Institute Virus-like micrograins and process for producing the same
US7449188B2 (en) 2001-01-18 2008-11-11 Vlaams Interuniversitair Instituut Voor Biotechnologie Recombinant oligometric protein complexes with enhanced immunogenic potential
AU2002324973A1 (en) 2001-09-14 2003-04-01 Fraunhofer-Gesellschaft Zur Forderung Der Angewandandten Forschung E.V. Immunoglobulin having particular framework scaffold and methods of making and using
CA2492097C (en) 2002-02-13 2013-06-25 Wisconsin Alumni Research Foundation Signal for packaging of influenza virus vectors
WO2003068163A2 (en) 2002-02-14 2003-08-21 Novavax, Inc. Optimization of gene sequences of chimeric virus-like particles for expression in insect cells
EP2319935B1 (en) 2002-03-19 2016-04-27 Stichting Dienst Landbouwkundig Onderzoek GnTIII (UDP-N-acetylglucosamine:Beta -D mannoside Beta (1,4)-N-acetylglucosaminyltransferase III) expression in plants.
AU2004235800B2 (en) 2003-05-05 2010-02-18 Dow Agrosciences Llc Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production
AU2004235813B2 (en) 2003-05-05 2010-07-29 Boyce Thompson Institute For Plant Research Vectors and cells for preparing immunoprotective compositions derived from transgenic plants
US7566458B2 (en) 2003-06-16 2009-07-28 Medimmune, Llc Influenza hemagglutinin and neuraminidase variants
US8592197B2 (en) 2003-07-11 2013-11-26 Novavax, Inc. Functional influenza virus-like particles (VLPs)
CN1560227A (zh) * 2004-02-25 2005-01-05 福建师范大学 雨生红球藻原生质体制备与再生技术
CN101065145B (zh) 2004-08-13 2010-12-15 印度科学工业研究所 嵌合g蛋白基狂犬疫苗
US9155483B2 (en) 2004-12-03 2015-10-13 The Invention Science Fund I, Llc Vision modification with reflected image
JP5343212B2 (ja) * 2005-03-15 2013-11-13 ヴェレニウム コーポレイション セルラーゼ、それらをコードする核酸、並びにそれらを作製及び使用する方法
PT2374892T (pt) 2005-04-29 2018-03-29 Univ Cape Town Expressão de proteínas virais em plantas
CN100410378C (zh) 2005-05-09 2008-08-13 中国农业科学院生物技术研究所 编码禽流感血凝素的基因及其植物表达载体和应用
EP2314705B1 (en) * 2005-06-24 2014-03-19 Bayer CropScience NV Methods for altering the reactivity of plant cell walls
JP2009502789A (ja) 2005-07-19 2009-01-29 ダウ グローバル テクノロジーズ インコーポレイティド 組み換えインフルエンザワクチン
US7871626B2 (en) 2005-08-04 2011-01-18 St. Jude Children's Research Hospital Modified influenza virus for monitoring and improving vaccine efficiency
RU2483751C2 (ru) 2005-10-18 2013-06-10 Новавакс, Инк. ФУНКЦИОНАЛЬНЫЕ ВИРУСОПОДОБНЫЕ ЧАСТИЦЫ ГРИППА (VLPs)
CA2630220C (en) * 2005-11-22 2020-10-13 Doris Coit Norovirus and sapovirus antigens
EP1984405A4 (en) 2006-02-13 2010-06-30 Fraunhofer Usa Inc INFLUENZA ANTIGENES, VACCINE COMPOSITIONS AND RELATED METHODS
EP2010210A4 (en) 2006-04-21 2010-07-21 Dow Agrosciences Llc VACCINE AGAINST BIRD FLUID AND METHOD OF APPLICATION THEREFOR
US8778353B2 (en) 2006-05-01 2014-07-15 Technovax, Inc. Influenza virus-like particle (VLP) compositions
DK2023952T3 (en) 2006-05-18 2015-10-19 Epimmune Inc Induction of immune responses to influenza virus using polypeptide and nucleic acid compositions
EP2029755A1 (en) 2006-05-22 2009-03-04 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
US20070286873A1 (en) 2006-05-23 2007-12-13 Williams John V Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor
WO2008088813A1 (en) 2007-01-17 2008-07-24 Lerner Medical Devices, Inc. Fiber optic phototherapy device
US7730950B2 (en) 2007-01-19 2010-06-08 Halliburton Energy Services, Inc. Methods for treating intervals of a subterranean formation having variable permeability
RU2499053C2 (ru) 2007-06-15 2013-11-20 Медикаго Инк. Способ синтеза белка с модифицированным профилем n-гликозилирования в растениях
KR100964462B1 (ko) 2007-07-10 2010-06-16 성균관대학교산학협력단 형질전환 식물 유래의 조류독감 바이러스 백신 및 그 제조방법
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
RU2358981C2 (ru) * 2007-08-07 2009-06-20 Центр "Биоинженерия" Российской Академии Наук Универсальная вакцина против вируса гриппа птиц
US20110059130A1 (en) 2007-08-20 2011-03-10 Fraunhofer Usa, Inc. Prophylactic and therapeutic influenza vaccines, antigens, compositions and methods
CN101422128B (zh) * 2007-10-29 2011-06-08 中国水产科学研究院黄海水产研究所 马尾藻原生质体的分离和再生
CN101978066A (zh) 2007-11-27 2011-02-16 麦迪卡格公司 表达血凝素之转基因植物中生产的重组流感病毒样颗粒(vlp)
EA201001064A1 (ru) 2007-12-28 2011-02-28 Юнилевер Н.В. Способ извлечения аромата из чая
US8799801B2 (en) 2008-01-16 2014-08-05 Qualcomm Incorporated Interactive ticker
KR101956910B1 (ko) 2008-01-21 2019-03-12 메디카고 인코포레이티드 헤마글루티닌을 발현하는 트랜스제닉 식물에서 생산된 재조합 인플루엔자 바이러스-유사 입자(ＶＬＰｓ)
CN102089432A (zh) 2008-07-08 2011-06-08 麦迪卡格公司 可溶性重组流感抗原
CN101626276A (zh) 2008-07-10 2010-01-13 华为技术有限公司 广告替换方法、装置及系统
ES2593277T3 (es) 2008-07-18 2016-12-07 Medicago Inc. Nuevo epítopo de inmunización contra el virus de la influenza
EP2328401B8 (en) 2008-08-27 2017-04-19 Arizona Board of Regents for and on behalf of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
US20100167376A1 (en) * 2008-10-06 2010-07-01 Genvault Corporation Methods for providing cellular lysates from cell wall-containing samples
WO2010077712A1 (en) 2008-12-09 2010-07-08 Novavax, Inc. Bovine respiratory syncytial virus virus-like particle (vlps)
CA2750409C (en) 2009-01-30 2019-03-05 Adlyfe, Inc. Peptide probes for amyloid beta protein
PT3431076T (pt) 2009-06-10 2021-10-26 Arbutus Biopharma Corp Formulação lipídica melhorada
LT2440231T (lt) 2009-06-10 2020-01-10 Nono Inc. Agento, prijungto prie tat internalizacijos peptido, įvedimas kartu su putliųjų ląstelių degranuliacijos inhibitoriumi
BRPI1015053A2 (pt) 2009-06-24 2019-07-09 Medicago Inc partículas similares a vírus da influenza quiméricas compreendendo hemaglutinina
TR201803560T4 (tr) 2009-09-22 2019-01-21 Medicago Inc Bitkiden türetilen proteinlerin hazırlanma metotları.
ES2614527T3 (es) 2010-11-04 2017-05-31 Medicago Inc. Sistema de expresión en plantas
JP6158088B2 (ja) 2010-11-05 2017-07-05 ノババックス，インコーポレイテッド 狂犬病糖タンパク質ウイルス様粒子（ｖｌｐ）
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法

Also Published As

Publication number Publication date
AU2010300033A1 (en) 2012-03-22
RU2567012C2 (ru) 2015-10-27
EP2480560B1 (en) 2018-02-21
PH12012500565B1 (en) 2018-01-12
AR078433A1 (es) 2011-11-09
KR101773431B1 (ko) 2017-09-12
PH12012500565A1 (en) 2012-10-22
US20130067807A1 (en) 2013-03-21
HRP20171564T1 (hr) 2017-11-17
KR20180026562A (ko) 2018-03-12
CA2772962A1 (en) 2011-03-31
ES2911037T3 (es) 2022-05-17
DK3354657T3 (da) 2022-04-19
EP3354657B1 (en) 2022-01-26
IL270661B (en) 2021-12-01
ES2642631T3 (es) 2017-11-17
JP2013505025A (ja) 2013-02-14
BR112012006415A2 (pt) 2016-11-16
JP2013505272A (ja) 2013-02-14
PT3354657T (pt) 2022-05-06
HRP20220478T1 (hr) 2022-05-27
PT2480658T (pt) 2017-11-13
SI2480560T1 (en) 2018-04-30
IN2012DN02637A (es) 2015-09-04
ES2662778T3 (es) 2018-04-09
NO2480560T3 (es) 2018-07-21
BR112012006414A8 (pt) 2017-10-10
AR078432A1 (es) 2011-11-09
HUE038557T2 (hu) 2018-10-29
PL2480560T3 (pl) 2018-06-29
KR20120072371A (ko) 2012-07-03
BR112012006414B1 (pt) 2020-09-15
JP5551780B2 (ja) 2014-07-16
NZ598481A (en) 2014-02-28
AU2010300033B2 (en) 2015-01-22
RU2012115661A (ru) 2013-10-27
IN2012DN02591A (es) 2015-08-28
SG178947A1 (en) 2012-04-27
SI2480658T1 (sl) 2017-11-30
PL2480658T3 (pl) 2017-12-29
NZ598508A (en) 2014-02-28
EP2480658A1 (en) 2012-08-01
ZA201202836B (en) 2012-12-27
IL218422B (en) 2018-03-29
CA2772962C (en) 2013-04-02
HRP20180509T1 (hr) 2018-05-04
HUE058165T2 (hu) 2022-07-28
CN102549008A (zh) 2012-07-04
EP2480560A1 (en) 2012-08-01
CN102549148A (zh) 2012-07-04
AU2010300034B2 (en) 2015-07-30
KR20190050862A (ko) 2019-05-13
KR102115226B1 (ko) 2020-05-27
EP2480658A4 (en) 2013-02-27
TR201803560T4 (tr) 2019-01-21
AU2010300034A1 (en) 2012-03-22
DK2480560T3 (en) 2018-04-16
CN107090021A (zh) 2017-08-25
BR112012006415B1 (pt) 2020-04-28
IL218422A0 (en) 2012-04-30
WO2011035423A1 (en) 2011-03-31
IL218393A0 (en) 2012-04-30
KR20120093223A (ko) 2012-08-22
CA2772964C (en) 2020-10-27
EP2480658B1 (en) 2017-09-06
DK2480658T3 (da) 2017-11-06
SI3354657T1 (sl) 2022-05-31
MX2012003372A (es) 2012-05-29
EP3354657A1 (en) 2018-08-01
MY164704A (en) 2018-01-30
WO2011035422A1 (en) 2011-03-31
IL257261A (en) 2018-03-29
ZA201202835B (en) 2015-08-26
US11826419B2 (en) 2023-11-28
HUE036776T2 (hu) 2018-07-30
SG178918A1 (en) 2012-04-27
RU2012115996A (ru) 2013-10-27
JP5554414B2 (ja) 2014-07-23
PH12012500566A1 (en) 2012-10-22
MY158475A (en) 2016-10-14
RU2579903C2 (ru) 2016-04-10
NO2480658T3 (es) 2018-02-03
CN107129973A (zh) 2017-09-05
US20120178149A1 (en) 2012-07-12
IL257261B (en) 2020-03-31
EP2480560A4 (en) 2013-01-23
CA2772964A1 (en) 2011-03-31
BR112012006414A2 (pt) 2016-11-16
US11833200B2 (en) 2023-12-05
PL3354657T3 (pl) 2022-05-09
PT2480560T (pt) 2018-04-03

Similar Documents

Publication Publication Date Title
IN2012DN02637A (es)
SG194724A1 (en) Lignin production from lignocellulosic biomass
PL2167631T3 (pl) Instalacja biogazu i sposób wytwarzania biogazu z odnawialnych surowców zawierających ligninę
PH12013500382A1 (en) Method for lignin recovery
WO2012126123A8 (en) Method of recovering plant-derived proteins
MY162541A (en) Method of manufacturing filter aid
WO2010080407A3 (en) Methods for increasing hydrolysis of cellulosic material
WO2012151509A3 (en) Lignin production from lignocellulosic biomass
PH12012502357A1 (en) Process for fractionation of biomass
MX2012001537A (es) Metodo para capturar bioxido de carbono producido por plantas de cemento al usar el ciclo de calcio.
ZA201107726B (en) Micro-alga belonging to genus navicula,process for production of oil by culture of the micro-alga,and oil collection from the micro-alga
WO2011051977A3 (en) Process for biodiesel production from a yeast strain
WO2010079069A3 (en) Process, plant, and biofuel from lignocellulosic feedstock
GB201012113D0 (en) Method for recovering molybdenum and cobalt
WO2010058427A3 (en) Process for production and purification of polymyxin b sulfate
NZ598995A (en) Producing edible residues from ethanol production
WO2011111050A3 (en) Methods of generating hydrogen
MY184409A (en) Production of bio-butanol and related products
WO2012057438A3 (ko) 1,3,5-트리옥산의 제조방법
WO2011057947A3 (en) Method for producing silicon
WO2011124815A3 (fr) Procede d'obtention d'un produit fermentescible a partir d'une biomasse lignocellulosique polluee
WO2012089843A3 (en) Extraction of scenedesmus cell components
TH124382A (th) วิธีการของการเตรียมอนุภาคคล้ายไวรัสซึ่งได้มาจากพืช
WO2009020101A1 (ja) 花の形態が改変された植物体の生産方法
WO2008143204A1 (ja) アブラヤシの樹幹を原料とするエタノールおよびディーゼル燃料の製造方法

Legal Events

Date Code Title Description
FG Grant or registration