[go: up one dir, main page]

BR9814404A - " clonagem de expressão em fungos filamentosos " - Google Patents

" clonagem de expressão em fungos filamentosos "

Info

Publication number
BR9814404A
BR9814404A BR9814404-9A BR9814404A BR9814404A BR 9814404 A BR9814404 A BR 9814404A BR 9814404 A BR9814404 A BR 9814404A BR 9814404 A BR9814404 A BR 9814404A
Authority
BR
Brazil
Prior art keywords
filamentous fungi
expression cloning
cloning
expression
interest
Prior art date
Application number
BR9814404-9A
Other languages
English (en)
Inventor
Johannes Maarten Van Den Brink
Gerard Cornelis Maria Selten
Johannes Petrus Theod Hombergh
Original Assignee
Dsm Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8229117&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9814404(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Nv filed Critical Dsm Nv
Publication of BR9814404A publication Critical patent/BR9814404A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Mushroom Cultivation (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

"CLONAGEM DE EXPRESSãO EM FUNGOS FILAMENTOSOS" São providos processos para isolar as seq³ências de DNA codificando proteínas com propriedades de interesse, por meio de clonagem de expressão em células hospedeiras de fungos filamentosos. As seq³ências de DNA isoladas são úteis nos processos para produção de proteínas de interesse.
BR9814404-9A 1997-12-22 1998-12-22 " clonagem de expressão em fungos filamentosos " BR9814404A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97204079 1997-12-22
PCT/EP1998/008577 WO1999032617A2 (en) 1997-12-22 1998-12-22 Expression cloning in filamentous fungi

Publications (1)

Publication Number Publication Date
BR9814404A true BR9814404A (pt) 2000-10-10

Family

ID=8229117

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9814404-9A BR9814404A (pt) 1997-12-22 1998-12-22 " clonagem de expressão em fungos filamentosos "

Country Status (13)

Country Link
US (1) US20020155536A1 (pt)
EP (2) EP1042461B1 (pt)
JP (3) JP4410413B2 (pt)
CN (1) CN1198924C (pt)
AT (1) ATE367439T1 (pt)
AU (1) AU736111C (pt)
BR (1) BR9814404A (pt)
CA (1) CA2313445C (pt)
DE (1) DE69838106T2 (pt)
DK (1) DK1042461T3 (pt)
ES (1) ES2287989T3 (pt)
PL (1) PL341760A1 (pt)
WO (1) WO1999032617A2 (pt)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP1224273B1 (en) 1999-10-01 2007-10-17 Novozymes A/S Enzyme granulate
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
DK1272669T3 (da) * 2000-04-13 2009-07-13 Dyadic Internat Usa Inc High-throughput screening af udtrykte DNA-biblioteker i trådsvampe
NZ524814A (en) 2000-09-21 2004-08-27 Basf Ag Talaromyces xylanases
DK1339837T3 (da) 2000-12-07 2008-06-09 Dsm Ip Assets Bv Prolyl-endoprotease fra Aspergillus Niger
WO2003054186A1 (en) 2001-12-21 2003-07-03 Dsm Ip Assets B.V. New rennets
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
AU2003219956A1 (en) * 2003-02-27 2004-09-28 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP1776461A2 (en) 2004-04-16 2007-04-25 DSMIP Assets B.V. Fungal promoters for expressing a gene in a fungal cell
CA2565888C (en) * 2004-05-27 2014-10-14 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a dna library
EA014853B1 (ru) * 2004-10-15 2011-02-28 ДСМ АйПи АССЕТС Б.В. Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида
JP2008527985A (ja) 2005-01-24 2008-07-31 ディーエスエム アイピー アセッツ ビー．ブイ． 糸状菌細胞において目的の化合物を産生させるための方法
JP2008532535A (ja) * 2005-03-18 2008-08-21 ディーエスエム アイピー アセッツ ビー．ブイ． 宿主細胞における改善された発現クローニング法
CA2630455A1 (en) 2005-11-29 2007-06-07 Dsm Ip Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
US8637280B2 (en) 2005-12-01 2014-01-28 Adcuram Nutrition Holding Gmbh Genes useful for the industrial production of citric acid
EA015925B1 (ru) 2006-06-29 2011-12-30 ДСМ АйПи АССЕТС Б.В. Способ получения полипептидов
EP2511372A1 (en) 2006-11-02 2012-10-17 DSM IP Assets B.V. Improved production of secreted proteins by filamentous fungi
WO2008073914A2 (en) 2006-12-10 2008-06-19 Dyadic International Inc. Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
AU2008214663B2 (en) 2007-02-15 2013-10-03 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
AU2008219305B2 (en) 2007-02-20 2013-08-22 Dsm Ip Assets B.V. Novel sialidase
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
EP2406372B1 (en) 2009-03-10 2017-08-23 DSM IP Assets B.V. Pregastric esterase and derivatives thereof
CN102741421B (zh) 2009-03-10 2015-03-25 帝斯曼知识产权资产管理有限公司 用于提高多肽产率的方法
WO2010121933A1 (en) 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
US8802415B2 (en) 2009-11-04 2014-08-12 Dsm Ip Assets B.V. Talaromyces transformants
CN103347997A (zh) 2010-02-04 2013-10-09 帝斯曼知识产权资产管理有限公司 制备具有性周期的丝状真菌菌株的方法和制备有性杂交丝状真菌菌株的方法
WO2011098577A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
DK2588616T3 (en) 2010-07-01 2019-03-11 Dsm Ip Assets Bv PROCEDURE FOR MAKING A RELATIONSHIP OF INTEREST
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
CN104508114A (zh) 2012-06-19 2015-04-08 帝斯曼知识产权资产管理有限公司 用于在细胞中表达基因的启动子
WO2014013074A1 (en) 2012-07-19 2014-01-23 Dsm Ip Assets B.V. Agse-deficient strain
EP3202900B1 (en) 2013-02-04 2018-12-12 DSM IP Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
US10487125B2 (en) 2013-12-02 2019-11-26 Dsm Ip Assets B.V. Ice structuring protein
CN105940103B (zh) * 2014-01-31 2021-06-15 株式会社Api 哌可酸4位羟化酶和利用该酶的4-羟基氨基酸的制造法
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
MX375798B (es) 2014-05-19 2025-03-07 Dsm Ip Assets Bv Endoproteasa específica para prolina.
WO2015177153A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
DK3242950T3 (da) 2015-01-06 2021-12-20 Dsm Ip Assets Bv Crispr-cas-system til en trådformet svampeværtscelle
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
MX2017015126A (es) 2015-06-02 2018-03-28 Dsm Ip Assets Bv Uso de proteina estructurante del hielo.
EP4361240A3 (en) 2015-11-17 2024-07-31 DSM IP Assets B.V. Preparation of a stable beer
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
SI3296401T1 (sl) * 2016-09-20 2020-07-31 Clariant International Ltd Metoda za selektivno vir ogljika-neodvisno ekspresijo protein-kodirajočih sekvenc v celici filamentozne glive
WO2017216392A1 (en) 2016-09-23 2017-12-21 Dsm Ip Assets B.V. A guide-rna expression system for a host cell
US10918113B2 (en) 2016-12-21 2021-02-16 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
CA3046153A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
CN110431224A (zh) 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 锌双核簇转录调控因子缺陷型菌株
WO2018127611A1 (en) 2017-04-06 2018-07-12 Dsm Ip Assets B.V. Self-guiding integration construct (sgic)
CN112424326A (zh) 2018-05-07 2021-02-26 帝斯曼知识产权资产管理有限公司 用于酶促油脱胶的方法
US12195769B2 (en) 2018-06-19 2025-01-14 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2021089452A1 (en) 2019-11-04 2021-05-14 Dsm Ip Assets B.V. Low volume transfection
JP2021093966A (ja) * 2019-12-18 2021-06-24 本田技研工業株式会社 糸状菌への遺伝子導入方法及びその利用
EP4359426A2 (en) 2021-06-24 2024-05-01 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2025012325A1 (en) 2023-07-11 2025-01-16 Dsm Ip Assets B.V. Protein arginine deiminase

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA2034487C (en) * 1989-06-16 2000-08-29 Virgil B. Lawlis Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
CA2063592C (en) * 1989-07-07 2003-01-21 Marco L. F. Giuseppin Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
JPH0595787A (ja) * 1991-10-04 1993-04-20 Jozo Shigen Kenkyusho:Kk ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または／及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びｃＤＮＡ遺伝子
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
JPH05268964A (ja) * 1992-03-30 1993-10-19 Meiji Milk Prod Co Ltd 糸状菌プロモーター
ATE238425T1 (de) * 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
JP3638657B2 (ja) * 1995-03-29 2005-04-13 株式会社資生堂 Ｄｎａ修復酵素
US5824485A (en) * 1995-04-24 1998-10-20 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
JP3343567B2 (ja) * 1995-06-29 2002-11-11 大関株式会社 糸状菌のエンハンサーｄｎａ塩基配列およびその用途
EP0873398B1 (en) * 1996-01-10 2005-04-20 Novozymes A/S A method for in vivo production of a mutant library in cells

Also Published As

Publication number Publication date
JP2002509698A (ja) 2002-04-02
DK1042461T3 (da) 2007-09-24
DE69838106T2 (de) 2008-04-03
EP1854890A1 (en) 2007-11-14
AU2515199A (en) 1999-07-12
EP1042461A2 (en) 2000-10-11
WO1999032617A2 (en) 1999-07-01
CA2313445C (en) 2012-03-20
US20020155536A1 (en) 2002-10-24
JP2005261438A (ja) 2005-09-29
CA2313445A1 (en) 1999-07-01
CN1198924C (zh) 2005-04-27
PL341760A1 (en) 2001-05-07
ES2287989T3 (es) 2007-12-16
JP4410413B2 (ja) 2010-02-03
ATE367439T1 (de) 2007-08-15
EP1042461B1 (en) 2007-07-18
DE69838106D1 (de) 2007-08-30
WO1999032617A3 (en) 1999-09-10
AU736111C (en) 2004-02-19
AU736111B2 (en) 2001-07-26
JP2010046080A (ja) 2010-03-04
CN1283227A (zh) 2001-02-07

Similar Documents

Publication Publication Date Title
BR9814404A (pt) " clonagem de expressão em fungos filamentosos "
ATE352626T1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
DE69930955D1 (de) Polypeptide mit lysophospholipaseaktivität und nukleinesäuren die sie kodieren
ATE484587T1 (de) Ldcam genannte moleküle
ATE184052T1 (de) Gdf-1 und uog1 proteine
ZA967173B (en) Purified coprinus laccases and nucleic acids and encoding same
BR9813608B1 (pt) célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase.
WO2001029222A3 (en) Polypeptides having phospholipase b activity and nucleic acids encoding same
PL1675956T3 (pl) Minimalna sekwencja DNA działająca jako insulator chromatyny i jej zastosowanie do wyrażania białek
DK1117798T3 (da) Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
DK0804558T3 (da) Tripeptidylaminopeptidase
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
DK1157100T3 (da) Oxaloacetathydrolase-defekte svampeværtsceller
ATE171212T1 (de) Corticotropin freisetzende rezeptoren des faktor 2
DE69619808D1 (de) Chimäre msp und daf proteine mit einem telloberflächenlokalisierendem domän
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
DE69828902D1 (de) Polypeptide mit 5-aminolevulinsäure synthaseaktivität und deren nukleinsäuren
BR0213773A (pt) Molécula de ácido nucleico isolada, vetor, célula hospedeira, e, método para a preparação de um composto de quìmica fina
ATE317896T1 (de) Polypeptide mit glucanotransferase aktivität und dafür kodierende nukleinsäuren
FI980782A0 (fi) En till epitel celler bindande protein region samt proteinet kodande DNA sekvens
BR0213772A (pt) Molécula de ácido nucleico isolada, vetor, célula hospedeira, e, método para a preparação de um composto de quìmica fina
DE69939587D1 (en) Semaphorin polypeptide
ATE547427T1 (de) Verfahren zur rekombinanten herstellung von ribonukleoproteinen
CA2360530A1 (en) A human nuclear protein having a ww domain and a polynucleotide encoding the protein

Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]