[go: up one dir, main page]

AR004940A1 - Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero. - Google Patents

Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero.

Info

Publication number
AR004940A1
AR004940A1 ARP960103001A AR10300196A AR004940A1 AR 004940 A1 AR004940 A1 AR 004940A1 AR P960103001 A ARP960103001 A AR P960103001A AR 10300196 A AR10300196 A AR 10300196A AR 004940 A1 AR004940 A1 AR 004940A1
Authority
AR
Argentina
Prior art keywords
sialic acid
cell culture
cell
culture
hospedant
Prior art date
Application number
ARP960103001A
Other languages
English (en)
Original Assignee
Hoffmann La Roche
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23863440&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR004940(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche, Genentech Inc filed Critical Hoffmann La Roche
Publication of AR004940A1 publication Critical patent/AR004940A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Rheumatology (AREA)
  • Pain & Pain Management (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La presente invencion se refiere a nuevos procedimientos para la preparacion de glicoproteinas mediante cultivos en celulas de mamiferos donde el contenido de acido sialico de la glicoproteina producida se controla en una amplia gama de va loresmanipulando el ambiente de cultivo de celulas. La invencion provee procedimientos en los cuales el contenido de acido sialico de la glicoproteina se modifica mediante cambios en los parametros de los cultivosde celulas que afectan la productivi dadespecifica de las celulas. Las realizaciones preferidas de la invencion incluyen procedimientos para elcultivo de celulas en las cuales se controla la osmolalidad del cultivo de celulas, asi como tambien la concentracion de un realzador detranscripcion durante la fase de produccion del cultivo de celulas.
ARP960103001A 1995-06-06 1996-06-06 Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero. AR004940A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/469,348 US5705364A (en) 1995-06-06 1995-06-06 Mammalian cell culture process

Publications (1)

Publication Number Publication Date
AR004940A1 true AR004940A1 (es) 1999-04-07

Family

ID=23863440

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP960103001A AR004940A1 (es) 1995-06-06 1996-06-06 Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero.
ARP980100640A AR011439A2 (es) 1995-06-06 1998-02-12 Procedimiento para producir quimeras de inmunoglobulina (ig) del receptor del factor de necrosis tumoral (tnfr), nuevas preparaciones igg1 del tnfr1 yuna composicion terapeutica que los contiene

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP980100640A AR011439A2 (es) 1995-06-06 1998-02-12 Procedimiento para producir quimeras de inmunoglobulina (ig) del receptor del factor de necrosis tumoral (tnfr), nuevas preparaciones igg1 del tnfr1 yuna composicion terapeutica que los contiene

Country Status (35)

Country Link
US (1) US5705364A (es)
EP (2) EP0832189B2 (es)
JP (2) JPH11507523A (es)
KR (1) KR100496356B1 (es)
CN (1) CN1166772C (es)
AR (2) AR004940A1 (es)
AT (2) ATE302266T1 (es)
AU (1) AU717847B2 (es)
BG (1) BG63213B1 (es)
BR (1) BR9609150A (es)
CA (1) CA2220684C (es)
CZ (1) CZ293719B6 (es)
DE (2) DE69635076T3 (es)
DK (2) DK1609853T4 (es)
EA (1) EA001215B1 (es)
ES (2) ES2248812T5 (es)
GE (1) GEP20012519B (es)
HU (1) HU226420B1 (es)
IL (1) IL122398A (es)
IS (1) IS2614B (es)
MY (1) MY113496A (es)
NO (1) NO322276B1 (es)
NZ (1) NZ310202A (es)
OA (1) OA10753A (es)
PL (1) PL185484B1 (es)
PT (1) PT1609853E (es)
RO (1) RO120267B1 (es)
SA (1) SA96170351B1 (es)
SI (2) SI1609853T2 (es)
SK (1) SK282658B6 (es)
TR (1) TR199701543T1 (es)
TW (2) TW426734B (es)
UA (1) UA47428C2 (es)
WO (1) WO1996039488A1 (es)
ZA (1) ZA964776B (es)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59010908D1 (de) 1989-09-12 2000-08-10 Hoffmann La Roche TNF-bindende Proteine
US5705364A (en) † 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US6656466B1 (en) * 1995-06-06 2003-12-02 Genetech, Inc. Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition
JP4306813B2 (ja) 1995-09-19 2009-08-05 アスビオファーマ株式会社 動物細胞の新規培養方法
CN1233189A (zh) * 1996-05-08 1999-10-27 弗·哈夫曼-拉罗切有限公司 用TNFR-Ig治疗哮喘
ATE309350T1 (de) * 1997-12-03 2005-11-15 Roche Diagnostics Gmbh Verfahren zur herstellung von polypeptiden mit geeigneter glykosilierung
US6140445A (en) * 1998-04-17 2000-10-31 Crompton Corporation Silane functional oligomer
US6528286B1 (en) * 1998-05-29 2003-03-04 Genentech, Inc. Mammalian cell culture process for producing glycoproteins
AU4314299A (en) * 1998-05-29 1999-12-13 Genentech Inc. Cell culture process for producing glycoproteins
TR200504220T2 (tr) 1998-12-17 2007-04-24 Biogen Idec Ma Inc. Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem.
GB9828624D0 (en) * 1998-12-23 1999-02-17 Glaxo Group Ltd Production of proteins
KR100475417B1 (ko) * 1998-12-30 2005-07-18 씨제이 주식회사 시알산 함량이 높은 재조합 당단백질의 제조방법
US7294481B1 (en) 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
AU778046B2 (en) 1999-04-26 2004-11-11 Genentech Inc. Cell culture process for glycoproteins
US6261805B1 (en) * 1999-07-15 2001-07-17 Boyce Thompson Institute For Plant Research, Inc. Sialyiation of N-linked glycoproteins in the baculovirus expression vector system
KR100394474B1 (ko) * 1999-12-18 2003-08-09 동아제약 주식회사 동물세포에서 재조합 당단백질의 연속식 대량생산 방법
ATE313554T1 (de) 2000-05-16 2006-01-15 Lipoxen Technologies Ltd Derivatisierung von proteinen in wässrigem lösungsmittel
WO2002002793A1 (fr) * 2000-07-05 2002-01-10 Japan As Represented By Secretary Of Osaka University Processus de production de glycoproteine
US20030040095A1 (en) * 2001-03-16 2003-02-27 Achille Arini Method for the production of pharmaceutically active recombinant proteins
US20030087372A1 (en) * 2001-06-13 2003-05-08 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
US6872549B2 (en) * 2002-03-27 2005-03-29 Immunex Corporation Methods for increasing polypeptide production
US20030190710A1 (en) * 2002-03-28 2003-10-09 Devries Ruth L. Control of glycoforms in IgG
US7067279B1 (en) * 2002-08-23 2006-06-27 Immunex Corporation Cell culture performance with betaine
US6974681B1 (en) 2002-08-23 2005-12-13 Immunex Corporation Cell culture performance with vanadate
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
AU2003298816C1 (en) * 2002-12-02 2010-12-16 Amgen Fremont, Inc. Antibodies directed to Tumor Necrosis Factor and uses thereof
AU2003300276B2 (en) * 2002-12-23 2010-07-01 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
DK1576182T4 (da) * 2002-12-23 2020-04-20 Bristol Myers Squibb Co Produktkvalitetsforbedring ved fremgangsmåde med dyrkning af pattedyreceller til proteinfremstilling
EP1620457A2 (en) * 2003-04-25 2006-02-01 Immunex Corporation Inducers of recombinant protein expression
KR101229989B1 (ko) 2003-10-24 2013-02-06 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
WO2005063813A2 (en) * 2003-12-23 2005-07-14 Applied Research Systems Ars Holding N.V. Process for the production of tumor necrosis factor-binding proteins
WO2005064002A1 (en) * 2003-12-31 2005-07-14 Samyang Genex Corporation Method for mass production of secondary metabolites in plant cell culture by treatment of an alkanoic acid or salt thereof
CN1926242B (zh) * 2004-02-13 2013-01-16 格莱克托普有限公司 高活性糖蛋白加工条件及其有效生产方法
US7294484B2 (en) * 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
TWI364458B (en) * 2004-08-27 2012-05-21 Wyeth Res Ireland Ltd Production of tnfr-lg
TWI374935B (en) * 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
MX2007014148A (es) * 2005-05-19 2008-01-11 Amgen Inc Composiciones y metodos para incrementar la estabilidad de anticuerpos.
CA2610315A1 (en) * 2005-06-03 2006-12-07 Biovitrum Ab (Publ) Process for cultivating animal cells comprising the feeding of plant-derived peptones
KR100670105B1 (ko) 2005-06-29 2007-01-17 주식회사 셀트리온 콩가수분해물의 저분자량 분획을 이용하여 시알산 함량이증가된 에리스로포이에틴을 생산하는 방법 및 그에 의하여생산된 시알산 함량이 증가된 에리스로포이에틴
US8470318B2 (en) 2005-11-07 2013-06-25 The Rockefeller University Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
CN106589132B (zh) 2005-12-20 2022-03-29 布里斯托尔-迈尔斯斯奎布公司 组合物和用于生产组合物的方法
US20070190057A1 (en) 2006-01-23 2007-08-16 Jian Wu Methods for modulating mannose content of recombinant proteins
US20070184065A1 (en) * 2006-01-26 2007-08-09 Jan Holgersson Compositions and Methods for Inhibiting Viral Adhesion
AU2007235413B2 (en) * 2006-04-05 2012-08-02 The Rockefeller University Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods
RU2463345C2 (ru) * 2006-07-13 2012-10-10 Вайет Получение гликопротеинов
RU2518289C2 (ru) 2006-09-13 2014-06-10 Эббви Инк, Способ получения антитела или его фрагмента с подпиткой (варианты)
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US20080145893A1 (en) * 2006-09-17 2008-06-19 Excellegene Sa Method for producing a recombinant protein at high specific productivity, high batch yield and high volumetric yield by means of transient transfection
EP2091969A4 (en) * 2006-10-27 2010-05-12 Univ Rockefeller POLYPEPTIDES WITH IMPROVED INFLAMMATORY INHIBITION AND REDUCED CYTOTOXIC PROPERTIES AND RELATED METHODS THEREFOR
WO2008069244A1 (ja) * 2006-12-05 2008-06-12 Kyowa Hakko Kogyo Co., Ltd. 糖蛋白質組成物の製造方法
RU2475751C2 (ru) * 2007-04-16 2013-02-20 Момента Фармасьютикалз, Инк. Способы, связанные с гликозилированием клеточной поверхности
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US20090023186A1 (en) * 2007-07-22 2009-01-22 Excellgene Sa Use of valproic acid for enhancing production of recombinant proteins in mammalian cells
WO2009023562A2 (en) * 2007-08-09 2009-02-19 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
TWI395593B (zh) * 2008-03-06 2013-05-11 Halozyme Inc 可活化的基質降解酵素之活體內暫時性控制
HUE067606T2 (hu) 2008-03-06 2024-10-28 Halozyme Inc Oldható hialuronidáz készítmény
ES2517603T3 (es) * 2008-04-07 2014-11-03 Bayer Healthcare, Llc Procedimientos de producción recombinante de glucoproteínas
US7846744B2 (en) 2008-04-22 2010-12-07 Ravetch Jeffrey V Methods of identifying anti-inflammatory compounds
MX2011002638A (es) 2008-09-15 2011-04-05 Genentech Inc Composiciones y metodos para regular osmolaridad celular.
NZ592097A (en) 2008-10-20 2013-01-25 Abbott Lab Viral inactivation during purification of il-12 and il-18 antibodies
TW201024318A (en) * 2008-10-20 2010-07-01 Abbott Lab Isolation and purification of antibodies using protein A affinity chromatography
DK3037529T3 (da) 2008-12-09 2019-05-20 Halozyme Inc Udvidede opløselige ph20-polypeptider og anvendelse deraf
EP2403523A1 (en) * 2009-03-06 2012-01-11 Halozyme, Inc. Temperature sensitive mutants of matrix metalloprotease 1 und uses thereof
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
US20110117087A1 (en) 2009-10-26 2011-05-19 Reinhard Franze Method for the production of a glycosylated immunoglobulin
WO2011062926A2 (en) 2009-11-17 2011-05-26 Medarex, Inc. Methods for enhanced protein production
WO2011068993A1 (en) * 2009-12-02 2011-06-09 Acceleron Pharma Inc. Compositions and methods for increasing serum half-life of fc fusion proteins.
AU2011220163B2 (en) * 2010-02-24 2015-05-28 Chiesi Farmaceutici S.P.A. Process for production and purification of recombinant lysosomal alpha-mannosidase
JPWO2012017925A1 (ja) 2010-08-02 2013-10-03 協和発酵キリン株式会社 物質の製造方法
US9879229B2 (en) 2011-03-14 2018-01-30 National Research Council Of Canada Method of viral production in cells
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
RU2580020C2 (ru) 2011-04-29 2016-04-10 Биокон Рисерч Лимитед Способ снижения гетерогенности антител и способ получения соответствующих антител
EP2718328A4 (en) 2011-06-08 2014-12-24 Acceleron Pharma Inc COMPOSITIONS AND METHODS FOR INCREASING THE HALF TIME OF SERUM
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
CN102839157A (zh) * 2012-07-12 2012-12-26 扬州大学 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
US9206390B2 (en) 2012-09-02 2015-12-08 Abbvie, Inc. Methods to control protein heterogeneity
NO2760138T3 (es) 2012-10-01 2018-08-04
US20140106405A1 (en) * 2012-10-15 2014-04-17 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
EP2906683B1 (en) 2012-10-15 2017-05-31 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
WO2015000865A1 (en) 2013-07-04 2015-01-08 F. Hoffmann-La Roche Ag Interference-suppressed immunoassay to detect anti-drug antibodies in serum samples
JP2016526385A (ja) 2013-07-06 2016-09-05 カディラ ヘルスケア リミティド モノクローナル抗体の改良された産生方法
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
SI3110961T1 (sl) * 2014-02-27 2020-03-31 F. Hoffmann-La Roche Ag Modulacija rasti celic in glikozilacije pri rekombinantni proizvodnji glikoproteina
JP6652334B2 (ja) 2014-05-31 2020-02-19 Ｊｃｒファーマ株式会社 ウリジンとｎ−アセチル−ｄ−マンノサミンとを含有する培地
EP3152318A1 (en) * 2014-06-03 2017-04-12 Lupin Limited Cell culture process for producing a protein
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
KR102007930B1 (ko) 2014-12-31 2019-08-06 주식회사 엘지화학 재조합 당단백질의 글리코실화 조절 방법
TWI797060B (zh) 2015-08-04 2023-04-01 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
JP7189767B2 (ja) 2015-11-19 2022-12-14 武田薬品工業株式会社 組換えヒトｃ１エステラーゼインヒビター及びその使用
RU2724536C2 (ru) * 2015-12-29 2020-06-23 Н.В. Нютрисиа Ферментированная молочная смесь с неперевариваемыми олигосахаридами
JP7471220B2 (ja) * 2017-11-30 2024-04-19 エフ・ホフマン－ラ・ロシュ・アクチェンゲゼルシャフト 哺乳動物細胞の培養方法
AU2019379858B2 (en) 2018-11-13 2024-01-04 Janssen Biotech, Inc. Control of trace metals during production of anti-CD38 antibodies
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
EP3906249A1 (en) 2019-12-06 2021-11-10 Regeneron Pharmacetucals, Inc. Anti-vegf protein compositions and methods for producing the same
KR20230008830A (ko) 2020-05-08 2023-01-16 리제너론 파마슈티칼스 인코포레이티드 Vegf 트랩 및 미니-트랩 및 안구 장애 및 암의 치료 방법
US12163122B2 (en) 2020-08-31 2024-12-10 Regeneron Pharmaceuticals, Inc. Asparagine feed strategies to improve cell culture performance and mitigate asparagine sequence variants
CN116761880A (zh) 2021-01-20 2023-09-15 瑞泽恩制药公司 改进细胞培养物中的蛋白质滴度的方法
US20230110811A1 (en) 2021-10-07 2023-04-13 Regeneron Pharmaceuticals, Inc. pH METER CALIBRATION AND CORRECTION
CN118076894A (zh) 2021-10-07 2024-05-24 里珍纳龙药品有限公司 pH建模和控制的系统及方法
US20230331776A1 (en) 2022-03-02 2023-10-19 Regeneron Pharmaceuticals, Inc. Manufacturing process for high titer antibody
WO2025219232A1 (en) 2024-04-15 2025-10-23 Evonik Operations Gmbh Application of small peptides supplemented cell culture media for improved cell performance

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926723A (en) 1974-08-08 1975-12-16 Massachusetts Inst Technology Method of controllably releasing glucose to a cell culture medium
JPS5938488B2 (ja) 1979-05-07 1984-09-17 株式会社東芝 空気調和機
CS251766B2 (en) * 1982-06-21 1987-08-13 Wellcome Found Method of interferon production
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4724206A (en) * 1984-02-13 1988-02-09 Damon Biotech, Inc. Protein production using hypertonic media
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
JPH0639919B2 (ja) 1985-08-29 1994-05-25 トヨタ自動車株式会社 過給機付き内燃機関の異常判別装置
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
WO1988001643A1 (en) 1986-08-29 1988-03-10 Endotronics, Inc. Method of culturing cells
JPH01257492A (ja) * 1987-03-05 1989-10-13 Green Cross Corp:The 異種蛋白質の生産増強方法
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
JPH066054B2 (ja) 1987-10-15 1994-01-26 帝人株式会社 動物細胞の培養方法
CA1312030C (en) * 1987-11-18 1992-12-29 Brian Maiorella Method to increase antibody titer
US5151359A (en) * 1988-05-19 1992-09-29 Mitsui Toatsu Chemicals Incorporated Method for producing of human tissue type plasminogen activator
WO1990003430A1 (en) 1988-09-23 1990-04-05 Cetus Corporation Cell culture medium for enhanced cell growth, culture longevity and product expression
KR0132666B1 (en) * 1989-03-14 1998-04-14 Hitachi Kk Method for controlling cultivation conditions for animal cells
JPH0396383A (ja) 1989-09-08 1991-04-22 Riso Kagaku Corp 画像形成装置
DE59010908D1 (de) 1989-09-12 2000-08-10 Hoffmann La Roche TNF-bindende Proteine
US5096816A (en) * 1990-06-05 1992-03-17 Cetus Corporation In vitro management of ammonia's effect on glycosylation of cell products through pH control
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
GB2251249B (en) * 1990-12-28 1995-06-21 Mogam Biotech Res Inst High-density medium for animal cell culture
AU3144193A (en) * 1991-11-21 1993-06-15 Board Of Trustees Of The Leland Stanford Junior University Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases
US5447851B1 (en) * 1992-04-02 1999-07-06 Univ Texas System Board Of Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells
CA2123593C (en) * 1992-09-15 2000-03-14 Craig A. Smith Method of treating tnf-dependent inflammation using tumor necrosis factor antagonists
JP3523293B2 (ja) † 1993-07-19 2004-04-26 住友製薬株式会社 インターフェロンの産生増強法
US5705364A (en) † 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
JP5515221B2 (ja) † 2008-01-28 2014-06-11 日油株式会社 ポリオキシエチレンソルビタン脂肪酸エステルの製造方法

Also Published As

Publication number Publication date
PL185484B1 (pl) 2003-05-30
EP0832189A1 (en) 1998-04-01
OA10753A (en) 2002-12-11
TW426734B (en) 2001-03-21
EP1609853B2 (en) 2020-01-22
BG102101A (en) 1998-07-31
HU226420B1 (en) 2008-12-29
CN1166772C (zh) 2004-09-15
JP4348376B2 (ja) 2009-10-21
IS4626A (is) 1997-12-03
AR011439A2 (es) 2000-08-16
RO120267B1 (ro) 2005-11-30
ZA964776B (en) 1997-12-08
CA2220684A1 (en) 1996-12-12
KR19990022473A (ko) 1999-03-25
HK1087151A1 (en) 2006-10-06
ES2248812T3 (es) 2006-03-16
ES2324046T5 (es) 2020-09-14
ATE302266T1 (de) 2005-09-15
DE69637867D1 (de) 2009-04-23
NZ310202A (en) 1999-10-28
MX9709452A (es) 1998-06-30
GEP20012519B (en) 2001-08-27
SI1609853T1 (sl) 2009-08-31
JP2007190024A (ja) 2007-08-02
ES2324046T3 (es) 2009-07-29
SK282658B6 (sk) 2002-11-06
CZ390997A3 (cs) 1998-06-17
CN1186514A (zh) 1998-07-01
PT1609853E (pt) 2009-06-09
JPH11507523A (ja) 1999-07-06
DE69635076T2 (de) 2006-06-01
AU6095296A (en) 1996-12-24
ES2248812T5 (es) 2008-12-16
US5705364A (en) 1998-01-06
BR9609150A (pt) 1999-02-23
EP0832189B1 (en) 2005-08-17
DK0832189T4 (da) 2008-10-27
UA47428C2 (uk) 2002-07-15
IS2614B (is) 2010-04-15
CZ293719B6 (cs) 2004-07-14
EP1609853A1 (en) 2005-12-28
NO975674D0 (no) 1997-12-05
DK0832189T3 (da) 2005-11-21
EA199700364A1 (ru) 1998-04-30
ATE425245T2 (de) 2009-03-15
MY113496A (en) 2002-03-30
TW516962B (en) 2003-01-11
HUP9900920A3 (en) 2001-06-28
DK1609853T4 (da) 2020-04-06
IL122398A0 (en) 1998-06-15
EP0832189B2 (en) 2008-07-09
DK1609853T3 (da) 2009-06-02
NO975674L (no) 1998-02-06
SI0832189T2 (sl) 2008-12-31
SI0832189T1 (sl) 2006-02-28
PL323737A1 (en) 1998-04-14
EA001215B1 (ru) 2000-12-25
AU717847B2 (en) 2000-04-06
DE69635076D1 (de) 2005-09-22
KR100496356B1 (ko) 2005-11-25
SI1609853T2 (sl) 2020-07-31
TR199701543T1 (xx) 1998-05-21
CA2220684C (en) 2002-11-12
IL122398A (en) 2001-12-23
HUP9900920A2 (hu) 1999-07-28
SA96170351B1 (ar) 2006-07-04
DE69635076T3 (de) 2009-01-15
SK167097A3 (en) 1998-07-08
EP1609853B1 (en) 2009-03-11
WO1996039488A1 (en) 1996-12-12
BG63213B1 (bg) 2001-06-29
HK1017903A1 (en) 1999-12-03
NO322276B1 (no) 2006-09-04

Similar Documents

Publication Publication Date Title
AR004940A1 (es) Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero.
Scoma et al. Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant
US11639494B2 (en) Culture medium
EA201270469A1 (ru) Способы культивирования клеток млекопитающих для получения белка
MX172674B (es) Procedimiento mejorado para la produccion de la hormona de crecimiento humano, mediante transformantes bacterianos
DE60044310D1 (de) Verfahren zur Herstellung von Oligosacchariden
BR0316882A (pt) Melhora na qualidade de produtos em processos de cultura de células de mamìferos para a produção de proteìnas
EP0573107A3 (en) Process for the production of virus vaccine from attenuated Varicella zoster viruses.
SI1428878T1 (sl) Postopek za proizvodnjo in čiščenje eritropoietina
CY1107771T1 (el) Μεθοδος για την παραγωγη χονδρινων εμφυτευματων απο in vitro καλλιεργημενα χονδροκυτταρα
FI93310B (fi) Menetelmä rokotteen valmistamiseksi piluksia kantavien nisäkkään kudoksiin tarttuvien bakteerien aiheuttamia tauteja vastaan
AR061976A1 (es) Produccion de glicoproteinas
ES2133163T3 (es) Esteres (o tioesteres) del acido n-acilsulfamico y esteres (o tioesteres) del acido n-sulfonilcarbamico como agentes hipercolesterolemicos.
Renvoisé et al. Emerging themes of ER organization in the development and maintenance of axons
Roos et al. Biochemical studies on cell fusion. II. Control of fusion response by lipid alteration.
ES2556454T3 (es) Proceso para producción de proteínas
Borré et al. Production of lytic plaques of viral origin in Penicillium
GB1198071A (en) Improvements in or relating to Prostaglandin Analogues and the Manufacture thereof
Voccoli et al. Mitochondrial remodeling in differentiating neuroblasts
Anderson et al. Acrosome morphogenesis in Lumbricus terrestris
Kimelberg et al. Cell growth and ouabain-sensitive 86Rb+ uptake and (Na++ K+)-ATPase activity in 3T3 and SV40 transformed 3T3 fibroblasts
BR112023003273A2 (pt) Estratégias de alimentação de asparagina para melhorar desempenho da cultura celular e mitigar variantes da sequência de asparagina
Nakamura et al. Ultrastructure of Chlamydomonas eugametos palmelloids induced by chloroplatinic acid treatment
CN106635972B (zh) 成纤维细胞的培养基及其制备方法
CN102517347A (zh) 一种采用半连续发酵工艺制备葡萄糖酸钠的方法

Legal Events

Date Code Title Description
FG Grant, registration