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MX9703012A - Proceso para la produccion bacteriana de polipeptidos heterologos con enlaces disulfuro. - Google Patents

Proceso para la produccion bacteriana de polipeptidos heterologos con enlaces disulfuro.

Info

Publication number
MX9703012A
MX9703012A MX9703012A MX9703012A MX9703012A MX 9703012 A MX9703012 A MX 9703012A MX 9703012 A MX9703012 A MX 9703012A MX 9703012 A MX9703012 A MX 9703012A MX 9703012 A MX9703012 A MX 9703012A
Authority
MX
Mexico
Prior art keywords
heterologous polypeptide
nucleic acid
acid encoding
dsba
dsbc protein
Prior art date
Application number
MX9703012A
Other languages
English (en)
Inventor
John C Joly
James R Swartz
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX9703012A publication Critical patent/MX9703012A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporciona un proceso para la produccion de un polipéptido heterologo en bacterias, cuyo proceso comprende: (a) el cultivo de células bacterianas, cuyas células comprenden al ácido nucleico que codifica para una proteína DsbA o DsbC, el ácido nucleico que codifica el polipéptido heterologo, una secuencia de señal para la secrecion de la proteína DsbA o DsbC y el polipéptido heterologo, y un promotor inducible para el ácido nucleico que codifica para la proteína DsbA y DsbC y ácido nucleico que codifica para el polipéptido heterologo, bajo condiciones mediante las cuales se induce la expresion del ácido nucleico que codifica para la proteína DsbA o DsbC antes de la induccion de la expresion del ácido nucleico que codifica para el polipéptido heterologo, y bajo condiciones mediante las cuales el polipéptido heterologo y la proteína DsbA o DsbC son secretados hacia el periplasma de la bacteria, o el polipéptido heterologo es secretado hacia el medio en el cual se cultivan las células bacterianas, y (b) la recuperacion del polipéptido heterologo a partir del periplasma o del medio de cultivo.
MX9703012A 1994-11-03 1995-10-11 Proceso para la produccion bacteriana de polipeptidos heterologos con enlaces disulfuro. MX9703012A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/333,912 US5639635A (en) 1994-11-03 1994-11-03 Process for bacterial production of polypeptides
PCT/US1995/014623 WO1996014422A1 (en) 1994-11-03 1995-10-11 Process for bacterial production of heterologous polypeptides with disulphide bonds

Publications (1)

Publication Number Publication Date
MX9703012A true MX9703012A (es) 1997-07-31

Family

ID=23304776

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9703012A MX9703012A (es) 1994-11-03 1995-10-11 Proceso para la produccion bacteriana de polipeptidos heterologos con enlaces disulfuro.

Country Status (11)

Country Link
US (1) US5639635A (es)
EP (1) EP0786009B1 (es)
JP (1) JP3749257B2 (es)
AT (1) ATE318917T1 (es)
CA (1) CA2203373C (es)
DE (1) DE69534822T2 (es)
DK (1) DK0786009T3 (es)
ES (1) ES2259440T3 (es)
IL (1) IL115834A (es)
MX (1) MX9703012A (es)
WO (1) WO1996014422A1 (es)

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